Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,471 G→C 18.3% T712T (ACC→ACG)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4710G→C18.3% 166.2 ‑3.6 ‑0.8408T712T (ACC→ACG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (24/51):  ref base (155/178):  total (179/229)
Fisher's exact test strand distribution p-value = 2.81e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.64e-01

Only 100 of 439 total aligned reads displayed.

CGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCA  >  NC_014034/681371‑681571
                                                                                                    |                                                                                                    
 gcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTgg                                                                                                     >  2:5608534/1‑101 (MQ=255)
   cTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTc                                                                                                   <  1:9178210/101‑1 (MQ=255)
    tGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCa                                                                                                  >  1:6250061/1‑101 (MQ=255)
     gAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAg                                                                                                 <  1:1632773/101‑1 (MQ=255)
     gAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAg                                                                                                 >  2:5061056/1‑101 (MQ=255)
      aaaGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgc                                                                                                <  1:8897671/101‑1 (MQ=255)
      aaaGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgc                                                                                                <  2:5162973/101‑1 (MQ=255)
       aaGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgcg                                                                                               >  1:3768247/1‑101 (MQ=255)
           ccGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGcc                                                                                           >  1:3441891/1‑101 (MQ=255)
             ggCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCAGCGATCTGCGTGCTGGTCAGCGCGCCGa                                                                                         >  2:7034659/1‑101 (MQ=255)
               ccATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATc                                                                                       <  1:791302/101‑1 (MQ=255)
                cATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCt                                                                                      <  1:266879/101‑1 (MQ=255)
                cATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCt                                                                                      <  2:8467935/101‑1 (MQ=255)
                  tCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTgc                                                                                    <  1:5825030/101‑1 (MQ=255)
                  tCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTgc                                                                                    <  2:1826129/101‑1 (MQ=255)
                  gcggggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTgc                                                                                    <  2:4612717/97‑1 (MQ=255)
                   cGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTgcg                                                                                   >  1:5484992/1‑101 (MQ=255)
                    gTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTgcgg                                                                                  >  1:4974726/1‑100 (MQ=255)
                    gTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTgcgc                                                                                  >  2:2745817/1‑101 (MQ=255)
                     tGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGcc                                                                                 >  2:3773238/1‑101 (MQ=255)
                      ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCg                                                                                <  1:5125290/101‑1 (MQ=255)
                          gAGCCCAGCCGGGAGAGCGCTGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGcc                                                                            <  1:3186230/101‑1 (MQ=255)
                           agcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGccc                                                                           <  2:8616827/101‑1 (MQ=255)
                            gcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCa                                                                          <  2:3225463/101‑1 (MQ=255)
                              ccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGa                                                                        <  1:7957038/101‑1 (MQ=255)
                                    cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGcgcc                                                                  >  2:8354755/1‑101 (MQ=255)
                                     ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGAGGcgccc                                                                 >  1:4202006/1‑100 (MQ=255)
                                     ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGcgccg                                                                 >  2:4184034/1‑101 (MQ=255)
                                     ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGcgccg                                                                 >  2:5774976/1‑101 (MQ=255)
                                     ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGcgccg                                                                 >  2:5968542/1‑101 (MQ=255)
                                      gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGcgccgc                                                                >  1:433832/1‑101 (MQ=255)
                                       agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCgccgcc                                                               >  1:4885152/1‑101 (MQ=255)
                                        gagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCt                                                              <  2:7796613/101‑1 (MQ=255)
                                        gagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCt                                                              >  2:8078913/1‑101 (MQ=255)
                                        gagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCt                                                              >  1:8500082/1‑101 (MQ=255)
                                         agCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTg                                                             <  1:8753038/101‑1 (MQ=255)
                                         agCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTg                                                             <  1:1810977/101‑1 (MQ=255)
                                         agCGCCGTCGCGCTGATCTGCGCCAGACCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATGTGCGCCGTGCCCAGACGCGCCGCCTg                                                             <  2:2518776/101‑1 (MQ=255)
                                          gcgcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTgc                                                            <  2:5484785/101‑1 (MQ=255)
                                              cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcg                                                        >  1:5373058/1‑101 (MQ=255)
                                              cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcg                                                        >  1:1254342/1‑101 (MQ=255)
                                                tCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtc                                                      <  1:387027/101‑1 (MQ=255)
                                                    gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                                  >  1:1413430/1‑101 (MQ=255)
                                                    gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTAGTAAGCGCGCCGATCTTCACCGAGCCCAGACGCGCCGCCTGCGCCgtcgtcg                                                  >  1:7355287/1‑100 (MQ=25)
                                                     cTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAg                                                 <  2:1901870/101‑1 (MQ=255)
                                                     cTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAg                                                 >  2:8540773/1‑101 (MQ=255)
                                                      tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgc                                                >  1:2064066/1‑101 (MQ=255)
                                                       gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                               >  2:6877291/1‑101 (MQ=255)
                                                       gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                               <  2:3935649/101‑1 (MQ=255)
                                                       gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                               >  1:2989495/1‑101 (MQ=255)
                                                       gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                               >  2:1026708/1‑101 (MQ=255)
                                                       gATCTGCCCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGAGCCGCCGTCAggg                                               >  2:2092657/1‑99 (MQ=255)
                                                           cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                           <  2:6207677/97‑1 (MQ=2)
                                                           cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                           <  1:7128055/97‑1 (MQ=2)
                                                           cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                           <  1:6682423/97‑1 (MQ=2)
                                                           cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                           <  2:7170955/97‑1 (MQ=2)
                                                           cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                           <  1:2384923/97‑1 (MQ=2)
                                                            gcgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACg                                          <  1:9040040/101‑1 (MQ=255)
                                                            gcgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACg                                          <  1:871275/101‑1 (MQ=255)
                                                              gcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                                        <  1:192119/101‑1 (MQ=255)
                                                               ccAGATCGGCGGTTTCCAGCCCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATg                                       <  2:7902759/101‑1 (MQ=9)
                                                               ccAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATg                                       >  1:7486580/1‑101 (MQ=255)
                                                               ccAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATg                                       <  2:7280903/101‑1 (MQ=255)
                                                                cAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGt                                      >  1:7688225/1‑101 (MQ=255)
                                                                cAGATCCGCCGCCTCACGCGCCGCGATCTGCTGACTGGATAGCGCGCCGATCTGCGCCGAGCCCTGCTGTGCCGTCTGCGGCGTCGTCTGTGCCACGaaca                                      >  2:972495/1‑98 (MQ=16)
                                                                 aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                     >  2:2181454/1‑101 (MQ=255)
                                                                  gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                    <  2:294039/101‑1 (MQ=255)
                                                                  gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                    >  1:294042/1‑101 (MQ=255)
                                                                   aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                   <  1:6381518/101‑1 (MQ=255)
                                                                    tCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                                  <  2:485663/101‑1 (MQ=255)
                                                                    tCCCCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                                  <  1:1011637/101‑1 (MQ=255)
                                                                      tgccgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                <  2:767235/100‑1 (MQ=255)
                                                                       gccgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGt                               <  2:8232806/101‑1 (MQ=255)
                                                                        cggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTg                              >  1:7098479/9‑101 (MQ=9)
                                                                         ggtttgcaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                             >  2:4529928/8‑101 (MQ=9)
                                                                         ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                             >  2:747594/8‑101 (MQ=9)
                                                                          gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                            >  1:2490793/7‑101 (MQ=9)
                                                                          gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                            <  2:139398/95‑1 (MQ=9)
                                                                          gccGCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                            <  1:1074174/101‑1 (MQ=255)
                                                                            ttccggcgcCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                          <  1:4663490/96‑3 (MQ=9)
                                                                             tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCCCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                         >  2:3681176/1‑101 (MQ=255)
                                                                              cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                        <  1:5707680/101‑1 (MQ=255)
                                                                               gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                       <  2:6468248/101‑1 (MQ=255)
                                                                               caGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgcggc                       <  1:8799047/100‑6 (MQ=9)
                                                                                aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                      <  1:6956006/101‑1 (MQ=9)
                                                                                aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                      >  1:4450472/1‑101 (MQ=9)
                                                                                   gccgcGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCcgcg                   >  2:2015913/1‑101 (MQ=255)
                                                                                   gccgcAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt                   >  1:6052557/1‑98 (MQ=2)
                                                                                    ccgcGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCgg                  <  1:8355118/101‑1 (MQ=255)
                                                                                    ccgcGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCgg                  >  2:637027/1‑101 (MQ=255)
                                                                                    ccgcGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCgg                  <  2:43347/101‑1 (MQ=255)
                                                                                     cgggATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCCCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGa                 <  2:6096918/98‑1 (MQ=255)
                                                                                        gATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg              <  2:2345395/101‑1 (MQ=255)
                                                                                        gATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg              >  2:1229520/1‑101 (MQ=255)
                                                                                         aTCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgc             >  2:1696301/1‑101 (MQ=255)
                                                                                           cTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGGGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg           <  1:8137418/101‑1 (MQ=255)
                                                                                               gTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt       >  2:7370179/1‑101 (MQ=255)
                                                                                               gTGCGGGTCAGCCCCCCGATCGGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt       <  2:2989397/101‑1 (MQ=37)
                                                                                                   tGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc   <  1:3609888/101‑1 (MQ=255)
                                                                                                    ggTCAGCTCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa  <  2:8995772/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCA  >  NC_014034/681371‑681571

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG