Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,491 C→T 18.8% A706T (GCG→ACG)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4910C→T18.8% 187.0 ‑2.7 0.0396A706T (GCG→ACG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (24/51):  ref base (141/180):  total (165/231)
Fisher's exact test strand distribution p-value = 6.87e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 427 total aligned reads displayed.

GTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAA  >  NC_014034/681391‑681591
                                                                                                     |                                                                                                    
 tGGCCGAGCCCAGCCCGGAGCGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGcc                                                                                                     <  1:8820805/101‑1 (MQ=255)
  ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCg                                                                                                    >  1:810629/1‑101 (MQ=255)
   gCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGa                                                                                                   >  1:5540303/1‑101 (MQ=255)
        gcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCa                                                                                              <  2:3225463/101‑1 (MQ=255)
         cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAg                                                                                             <  2:5118593/101‑1 (MQ=255)
         cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAg                                                                                             <  1:6293354/101‑1 (MQ=255)
          ccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGa                                                                                            <  2:4325195/101‑1 (MQ=255)
              cccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGAcgcg                                                                                        <  2:7673715/101‑1 (MQ=255)
                cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGcgcc                                                                                      >  2:8354755/1‑101 (MQ=255)
                 ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGAGGcgccc                                                                                     >  1:4202006/1‑100 (MQ=255)
                 ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGcgccg                                                                                     >  2:5774976/1‑101 (MQ=255)
                 ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGcgccg                                                                                     >  2:5968542/1‑101 (MQ=255)
                  gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGcgccgc                                                                                    >  2:6339652/1‑101 (MQ=255)
                  gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGcgccgc                                                                                    >  1:433832/1‑101 (MQ=255)
                   agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCgccgcc                                                                                   >  1:1468991/1‑101 (MQ=255)
                   agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCgccgcc                                                                                   >  1:1986315/1‑101 (MQ=255)
                   agagCGCCGCCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCgccgcc                                                                                   >  2:7502971/1‑101 (MQ=255)
                    gagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCt                                                                                  <  1:7152044/101‑1 (MQ=255)
                         ccGTCGCGCTTATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCGGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGcc                                                                             <  2:2078072/101‑1 (MQ=255)
                          cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcg                                                                            <  2:6103681/101‑1 (MQ=255)
                          cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcg                                                                            <  2:1879837/101‑1 (MQ=255)
                            tCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtc                                                                          <  1:3834428/101‑1 (MQ=255)
                            tCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtc                                                                          >  1:2967200/1‑101 (MQ=255)
                            tCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtc                                                                          <  2:8106648/101‑1 (MQ=255)
                              gcgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcgt                                                                        <  2:8547386/101‑1 (MQ=255)
                              gcgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcgt                                                                        >  1:5211495/1‑101 (MQ=255)
                              gcgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcgc                                                                        >  2:9027387/1‑100 (MQ=255)
                               cgcTGATCTGCGCCAGCCCCGCCGCCTCGAGCGCCGCGATCTGCGGGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                                                       <  2:4124846/101‑1 (MQ=255)
                                gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTAGTAAGCGCGCCGAT.CTTCACCGAGCCCAGACGCGCCGCCTGCGCCgtcgtcg                                                                      >  1:7355287/1‑100 (MQ=25)
                                  tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgc                                                                    <  2:4575151/101‑1 (MQ=255)
                                  tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgc                                                                    >  1:2340595/1‑101 (MQ=255)
                                   gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                                   >  1:5826934/1‑101 (MQ=255)
                                   gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                                   >  2:6877291/1‑101 (MQ=255)
                                   gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                                   >  2:1026708/1‑101 (MQ=255)
                                   gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                                                   >  1:2923956/1‑101 (MQ=255)
                                    agccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGAGCCGCCGCCTGCGCCGTCGTCAgcgc                                                                  >  1:8956321/8‑101 (MQ=9)
                                     gccgccccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGcc                                                                 <  2:5579992/95‑1 (MQ=2)
                                       cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                               <  2:7170955/97‑1 (MQ=2)
                                       cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                               <  1:6682423/97‑1 (MQ=2)
                                       ccggcCAGATCCGCCGCCTCGAGCGCCGCGCTCTGCGTGCTGGGCAGCGCGCCGAT.CTGCGCCGAGCCCAGCCGCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                               <  2:8853564/98‑1 (MQ=21)
                                        gcgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCATACGCGCCGCCTGCGCCGTCGTCAGCGCCACg                                                              <  2:9175464/101‑1 (MQ=255)
                                        gcgcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACg                                                              <  1:7919851/101‑1 (MQ=255)
                                        gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACg                                                              <  1:5068900/98‑1 (MQ=2)
                                           ccAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATg                                                           >  1:7486580/1‑101 (MQ=255)
                                           ccAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATg                                                           <  2:7280903/101‑1 (MQ=255)
                                           ccAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATg                                                           <  1:1830435/101‑1 (MQ=255)
                                            cAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGt                                                          >  1:7688225/1‑101 (MQ=255)
                                                tCCCCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                                                      <  1:1011637/101‑1 (MQ=255)
                                                  ggcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                                    <  2:292821/91‑1 (MQ=9)
                                                  ggcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                                    >  2:1737511/11‑101 (MQ=9)
                                                  cgccgcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                                    >  2:3791049/1‑101 (MQ=255)
                                                   gcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGt                                                   <  1:637135/92‑1 (MQ=9)
                                                   gccgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGt                                                   <  1:8590391/101‑1 (MQ=255)
                                                   gccgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGt                                                   <  2:8232806/101‑1 (MQ=255)
                                                    cggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTg                                                  >  1:7098479/9‑101 (MQ=9)
                                                    ccgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTg                                                  >  2:2207637/1‑101 (MQ=255)
                                                      gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                                >  1:2490793/7‑101 (MQ=9)
                                                      gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                                <  1:8483516/95‑1 (MQ=9)
                                                      gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                                <  2:139398/95‑1 (MQ=9)
                                                       tttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccg                                               >  2:705634/6‑100 (MQ=9)
                                                       ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCa                                               <  1:5411617/101‑1 (MQ=255)
                                                        ttccggcgcCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                                              <  1:4663490/96‑3 (MQ=9)
                                                        cTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaa                                              >  1:3240182/1‑101 (MQ=255)
                                                         tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                             >  2:7817180/1‑101 (MQ=255)
                                                         tCGAGCGCCGCGAGCTGCGTGCTGGGCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCGGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                             <  2:5276642/101‑1 (MQ=255)
                                                           gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                                           >  1:8281636/1‑101 (MQ=255)
                                                            aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                          >  1:4450472/1‑101 (MQ=9)
                                                            aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                          <  1:6956006/101‑1 (MQ=9)
                                                            aGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                          >  1:1259597/1‑101 (MQ=9)
                                                               gccgcAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt                                       >  1:6052557/1‑98 (MQ=2)
                                                                ccgcGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCgg                                      <  2:6429929/101‑1 (MQ=255)
                                                                ccgcAACCTGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                                      <  2:4843469/101‑5 (MQ=2)
                                                                ccgcAACCTGCGGGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                                      <  2:2626988/101‑5 (MQ=2)
                                                                   cgATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATc                                   >  2:4492151/1‑101 (MQ=255)
                                                                    gATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                  >  2:1229520/1‑101 (MQ=255)
                                                                    gATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                  <  2:2345395/101‑1 (MQ=255)
                                                                    gATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                  <  2:2851972/101‑1 (MQ=255)
                                                                      tCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc                                <  2:3768138/101‑1 (MQ=255)
                                                                        tGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccgc                              >  1:2557766/1‑101 (MQ=255)
                                                                        tGCGTGCTCGACAGCGCCAGAAG.CTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATcgcatt                              <  2:7437705/101‑4 (MQ=2)
                                                                           gTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCAGCCTGAGCCGTCGTCAGCGCAACGATGTAATCGGTGCCAAAGACGCAGATAGCCGCCGt                           >  1:9098958/1‑101 (MQ=25)
                                                                           gTGCGGGTCAGCCCCCCGAT.CGGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                           <  2:2989397/101‑1 (MQ=37)
                                                                               tGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc                       <  1:3609888/101‑1 (MQ=255)
                                                                               tGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc                       >  2:5038536/1‑101 (MQ=255)
                                                                                ggTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa                      >  1:3935235/1‑101 (MQ=255)
                                                                                ggTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa                      <  2:1986255/101‑1 (MQ=255)
                                                                                   cAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcg                   >  1:8788796/1‑101 (MQ=255)
                                                                                   cAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcg                   <  1:3777312/101‑1 (MQ=255)
                                                                                    aGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcgc                  >  2:8434274/1‑101 (MQ=255)
                                                                                        cgcCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCgccgcc              <  1:214712/101‑1 (MQ=255)
                                                                                           cGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAcc           <  2:2169105/101‑1 (MQ=255)
                                                                                           cGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAcc           >  2:8045282/1‑101 (MQ=255)
                                                                                            gAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCg          <  1:7785506/101‑1 (MQ=255)
                                                                                             aT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGt         >  2:3595212/1‑101 (MQ=255)
                                                                                              t.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTc        >  2:7897857/1‑101 (MQ=255)
                                                                                                cTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCg       >  2:5682143/1‑101 (MQ=255)
                                                                                                cTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCg       >  1:5734551/1‑101 (MQ=255)
                                                                                                  gcgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGcc     >  1:489967/1‑101 (MQ=255)
                                                                                                   cgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCg    >  1:1180572/1‑101 (MQ=255)
                                                                                                    gcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGa   >  1:3151372/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAT.CTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAA  >  NC_014034/681391‑681591

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG