Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,548 A→G 9.1% F687L (TTT→CTT)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,5480A→G 9.1% 59.0 0.0 ‑0.0336F687L (TTT→CTT) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (17/14):  ref base (164/141):  total (181/155)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.61e-01

Only 100 of 373 total aligned reads displayed.

TCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCG  >  NC_014034/681448‑681648
                                                                                                    |                                                                                                    
tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCCCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                                                                                      >  2:3681176/1‑101 (MQ=255)
  gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                                                                                                    <  1:4425093/101‑1 (MQ=255)
   aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                                                                                   >  2:8913765/1‑101 (MQ=9)
   aGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                                                                                   >  2:8113152/1‑101 (MQ=2)
    gcgcCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccc                                                                                                  >  2:3094648/1‑100 (MQ=2)
      gccgcGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCcgcg                                                                                                <  1:6338701/101‑1 (MQ=255)
       ccgcGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCgg                                                                                               <  2:43347/101‑1 (MQ=255)
       ccgcGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCgg                                                                                               <  2:6429929/101‑1 (MQ=255)
       ccgcGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCgg                                                                                               <  1:8355118/101‑1 (MQ=255)
       ccgcAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                                                                                               <  2:4843469/101‑5 (MQ=2)
       ccgcAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                                                                                               <  2:8317579/101‑5 (MQ=2)
       ccgcAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                                                                                               >  2:5708540/1‑97 (MQ=2)
         gcgATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                                                                                             <  1:2544415/101‑1 (MQ=255)
         gcgATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                                                                                             >  2:7974949/1‑101 (MQ=255)
          cgATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATc                                                                                            <  2:2037065/101‑1 (MQ=255)
           gATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                                                                           <  1:5461293/101‑1 (MQ=255)
           gATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                                                                           <  2:2345395/101‑1 (MQ=255)
            aTCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgc                                                                                          >  2:1696301/1‑101 (MQ=255)
            aTCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGGGCCACAGCCGCGGCTccc                                                                                          >  1:4285097/1‑99 (MQ=255)
             tCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc                                                                                         <  2:3768138/101‑1 (MQ=255)
                gCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCgccgcc                                                                                      <  1:7195830/101‑1 (MQ=255)
                  gTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                                                                                    >  1:2328972/1‑101 (MQ=255)
                  gTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                                                                                    <  1:6867195/101‑1 (MQ=255)
                   tGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTg                                                                                   >  2:1215133/1‑101 (MQ=255)
                    gCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGc                                                                                  >  1:7840939/1‑101 (MQ=255)
                    gCTGGTCACCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGc                                                                                  <  2:6115125/101‑1 (MQ=255)
                     cTGGTCGGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc                                                                                 <  2:7450661/101‑1 (MQ=255)
                      tGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc                                                                                <  2:5960864/101‑1 (MQ=255)
                      tGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc                                                                                >  2:5038536/1‑101 (MQ=255)
                      tGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc                                                                                <  1:5205440/101‑1 (MQ=255)
                        gTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAg                                                                              >  1:3874839/1‑101 (MQ=255)
                          cAGCGAGCCGATCTGGGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcg                                                                            >  1:8846220/1‑101 (MQ=255)
                           aGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcgc                                                                           >  1:4182010/1‑101 (MQ=255)
                            gcgcgcCGATCTGCGCCGAGCCCAGACGCGCCACCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgcc                                                                          <  2:5144656/101‑1 (MQ=255)
                               cgcCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCgccgcc                                                                       >  2:7074022/1‑101 (MQ=255)
                                 cgaaTCTGCGCCGGGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAc                                                                     <  2:2684192/98‑1 (MQ=255)
                                  cGATCGGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAcc                                                                    <  1:5317330/101‑1 (MQ=255)
                                   gATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCg                                                                   <  1:7785506/101‑1 (MQ=255)
                                    aTCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGt                                                                  >  2:3595212/1‑101 (MQ=255)
                                      cTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCg                                                                >  1:5734551/1‑101 (MQ=255)
                                      cTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCg                                                                <  1:6718068/101‑1 (MQ=255)
                                         cgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCg                                                             >  1:1180572/1‑101 (MQ=255)
                                          gcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGa                                                            >  1:3151372/1‑101 (MQ=255)
                                            cGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAg                                                          >  2:7833335/1‑101 (MQ=255)
                                            cGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAg                                                          <  1:5971680/101‑1 (MQ=255)
                                              aGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGcc                                                        >  1:2754991/1‑101 (MQ=255)
                                               gCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCa                                                       <  2:2521089/101‑1 (MQ=255)
                                                 ccAGACGGGCCGCCTGCGCCGTCGTCAGCGCCCCGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgc                                                     <  1:2513586/101‑1 (MQ=255)
                                                  cAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcg                                                    >  2:5865375/1‑101 (MQ=255)
                                                  cAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcg                                                    >  1:2418736/1‑101 (MQ=255)
                                                  cAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcg                                                    <  2:3240083/101‑1 (MQ=255)
                                                    gACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgcc                                                  >  1:342502/1‑101 (MQ=255)
                                                    gACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgcc                                                  <  1:4837606/101‑1 (MQ=255)
                                                     aCGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgccg                                                 >  2:4543121/1‑101 (MQ=255)
                                                      cgcgCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgccgc                                                <  2:2967102/101‑1 (MQ=255)
                                                         gccgccTGCTCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGAt                                             >  1:745272/1‑101 (MQ=255)
                                                         gccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCCGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGAt                                             >  1:6965086/1‑101 (MQ=255)
                                                         gccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGAt                                             >  2:3833750/1‑101 (MQ=255)
                                                         gccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGAt                                             >  1:7161502/1‑101 (MQ=255)
                                                         gccgccTGCGCCGTCGTCAGCGCCACAATGTCATCGGTGCCAAGGCCCTTGATCGCATTGCCACTCAGCGCCGCCACGCTGGCCGTATTCAGcgccgcaac                                             >  2:524886/1‑98 (MQ=18)
                                                          ccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGcgccgcaagc                                            >  1:1926776/1‑97 (MQ=25)
                                                          ccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATc                                            <  1:2982971/101‑1 (MQ=255)
                                                           cgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCt                                           >  1:4739194/1‑101 (MQ=255)
                                                           cgccTGCGCCGTCCTCGGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCt                                           <  1:3802731/101‑1 (MQ=255)
                                                            gccTGCGCCGTCGTCAGCGCCACGATGTCCTCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGTCCGcgcccggaccgc                                          >  2:7362025/1‑93 (MQ=25)
                                                            gccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTg                                          >  2:5411432/1‑101 (MQ=37)
                                                            gccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTg                                          <  1:6221706/101‑1 (MQ=255)
                                                            gccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTg                                          <  2:8767810/101‑1 (MQ=255)
                                                             ccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTgc                                         <  2:2789418/101‑1 (MQ=37)
                                                               tGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTgcgc                                       >  2:8479219/1‑101 (MQ=255)
                                                                    cgtcgtCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGc                                  >  2:8587670/1‑101 (MQ=255)
                                                                     gtcgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCt                                 >  1:7622101/1‑101 (MQ=255)
                                                                       cgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCa                               <  2:1468944/101‑1 (MQ=255)
                                                                       cgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCa                               >  1:1380914/1‑101 (MQ=255)
                                                                       cgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCa                               >  1:7713901/1‑101 (MQ=255)
                                                                        gtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAg                              <  2:7555766/101‑1 (MQ=255)
                                                                        gtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAg                              <  2:9019940/101‑1 (MQ=255)
                                                                        gtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAg                              <  1:1026726/101‑1 (MQ=255)
                                                                          cAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcg                            >  1:1653253/1‑101 (MQ=255)
                                                                          cAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcg                            >  1:6086420/1‑101 (MQ=255)
                                                                            gcgcCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgcc                          <  1:4466940/101‑1 (MQ=255)
                                                                             cgcCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccg                         <  1:8769863/101‑1 (MQ=255)
                                                                              gcCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgc                        >  2:1238650/1‑101 (MQ=255)
                                                                                cACGATGTCATCGGTGCCAAAGCCGCGGCTCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgcca                      >  1:3540459/1‑100 (MQ=255)
                                                                                   gATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCt                   >  2:1985806/1‑101 (MQ=255)
                                                                                    aTGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgcctgcca                  >  1:5636718/1‑96 (MQ=255)
                                                                                    aTGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTg                  >  2:7154260/1‑101 (MQ=255)
                                                                                    aTGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTg                  >  2:2094633/1‑101 (MQ=255)
                                                                                     tGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGc                 >  1:3630665/1‑101 (MQ=255)
                                                                                     tGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGc                 >  1:2884478/1‑101 (MQ=255)
                                                                                      gTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCtaga                >  1:2214056/1‑98 (MQ=255)
                                                                                      gTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCa                <  1:2124870/101‑1 (MQ=255)
                                                                                      gTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCa                >  1:1764776/1‑101 (MQ=255)
                                                                                        cATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCAcc              >  2:8857762/1‑101 (MQ=255)
                                                                                              tGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCa        >  1:3764640/1‑101 (MQ=255)
                                                                                                ccAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAg      >  1:8256303/1‑101 (MQ=255)
                                                                                                ccAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAg      <  2:1059939/101‑1 (MQ=255)
                                                                                                 cAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGTGCCGCCGCCTGCACCGAGCCAAGc     <  1:9027809/101‑1 (MQ=255)
                                                                                                  aaaGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGcc    >  1:8062035/1‑101 (MQ=255)
                                                                                                  aaaGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGcc    >  1:8501688/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCG  >  NC_014034/681448‑681648

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG