Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 74,095 A→C 100% F196L (TTT→TTG trmE ← tRNA modification GTPase TrmE

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_01403474,0950A→C100.0% 1756.6 465F196L (TTT→TTGtrmEtRNA modification GTPase TrmE
Reads supporting (aligned to +/- strand):  new base (227/238):  ref base (0/0):  total (227/238)

Only 100 of 482 total aligned reads displayed.

AGAAGCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGAAAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCAGCG  >  NC_014034/73997‑74192
                                                                                                  |                                                                                                 
aGAAGCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCCCCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGacaa                                                                                                 >  1:2175538/1‑101 (MQ=255)
  aaGCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACt                                                                                               <  1:2553668/101‑1 (MQ=255)
      gTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTgcgc                                                                                           >  1:1771975/1‑101 (MQ=255)
       tCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGcgcc                                                                                          <  2:2588338/101‑1 (MQ=255)
        cGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGcgccg                                                                                         >  1:4816682/1‑101 (MQ=255)
         gATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGcgccgc                                                                                        >  2:6909178/1‑101 (MQ=255)
         gATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTACCGCCGACAACTGcgccgc                                                                                        >  1:2728194/1‑101 (MQ=255)
         gATTTCCCGGCATACGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCTGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGcgccgc                                                                                        >  2:5522858/1‑101 (MQ=255)
          aTTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCa                                                                                       >  2:8489300/1‑101 (MQ=255)
             tCCGGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAAcc                                                                                    <  2:1650722/101‑1 (MQ=255)
             tCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAAcc                                                                                    >  2:359106/1‑101 (MQ=255)
               ccGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTc                                                                                  >  2:3760088/1‑101 (MQ=255)
                 ggCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCgg                                                                                <  1:4094889/101‑1 (MQ=255)
                 ggCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCgg                                                                                >  1:909341/1‑101 (MQ=255)
                   cATTCTGCCCCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAc                                                                              <  2:8266148/101‑1 (MQ=255)
                    aTTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAca                                                                             >  1:201192/1‑101 (MQ=255)
                     ttCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAcag                                                                            >  2:540051/1‑101 (MQ=255)
                      tCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAcagc                                                                           >  1:1599515/1‑101 (MQ=255)
                       cGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAcagca                                                                          >  2:5789193/1‑101 (MQ=255)
                       cGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAcagca                                                                          >  2:6243703/1‑101 (MQ=255)
                       cGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAcagca                                                                          <  1:2258791/101‑1 (MQ=255)
                        ggCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAcagcag                                                                         >  1:8415760/1‑101 (MQ=255)
                        ggCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAcagcag                                                                         <  1:7963756/101‑1 (MQ=255)
                         gccgccCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACagcagc                                                                        <  1:6151364/101‑1 (MQ=255)
                          ccgccCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAgcg                                                                       >  2:5117227/1‑101 (MQ=255)
                            gccCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCa                                                                     <  2:699136/101‑1 (MQ=255)
                              ccGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaa                                                                   <  1:5210640/101‑1 (MQ=255)
                              ccGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaa                                                                   >  1:1298435/1‑101 (MQ=255)
                               cgacgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaaa                                                                  <  1:4479218/101‑1 (MQ=255)
                                gacgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAg                                                                 >  1:2818510/1‑101 (MQ=255)
                                gacgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAg                                                                 <  2:2022230/101‑1 (MQ=255)
                                  cgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGcc                                                               >  1:3972031/1‑101 (MQ=255)
                                   gaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCt                                                              <  1:1003648/101‑1 (MQ=255)
                                      cGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCg                                                           <  2:6329077/101‑1 (MQ=255)
                                       gCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCgg                                                          <  2:8720235/101‑1 (MQ=255)
                                           cTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTc                                                      <  1:5432980/101‑1 (MQ=255)
                                            ttCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTca                                                     >  1:6973073/1‑101 (MQ=255)
                                             tCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTcac                                                    >  1:8913512/1‑101 (MQ=255)
                                             tCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTcac                                                    >  1:6044480/1‑101 (MQ=255)
                                              cAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTcaca                                                   >  1:3734523/1‑101 (MQ=255)
                                              cAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTcaca                                                   <  1:3854830/101‑1 (MQ=255)
                                              cAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTcaca                                                   >  1:7628458/1‑101 (MQ=255)
                                               aaaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACAt                                                  >  1:7144483/1‑101 (MQ=255)
                                               aaaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACAt                                                  >  2:7384265/1‑101 (MQ=255)
                                                aaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATc                                                 <  2:2171377/101‑1 (MQ=255)
                                                aaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATc                                                 >  1:5538452/1‑101 (MQ=255)
                                                aaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATc                                                 >  2:7453886/1‑101 (MQ=255)
                                                 aGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCa                                                <  1:8272253/101‑1 (MQ=255)
                                                   ccTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAAAGCAGCGCAAAAGCATCCGGCGTCACATCAAc                                              >  1:4404943/1‑101 (MQ=255)
                                                    cTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAAcc                                             <  1:6679660/101‑1 (MQ=255)
                                                       cccGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGc                                          >  2:4874140/1‑101 (MQ=255)
                                                         cGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAg                                        >  1:5104110/1‑101 (MQ=255)
                                                          gCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAgg                                       >  2:3273419/1‑101 (MQ=255)
                                                           cACTCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGt                                      >  1:7196101/1‑101 (MQ=255)
                                                           cACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAggc                                      >  2:8154012/1‑100 (MQ=255)
                                                           cACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGt                                      >  2:1012771/1‑101 (MQ=255)
                                                            ccccGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTc                                     <  2:3151636/100‑1 (MQ=255)
                                                            ccccGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTc                                     <  1:4911159/100‑1 (MQ=255)
                                                              ccGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCtt                                   >  2:6594644/1‑101 (MQ=255)
                                                              ccGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCtt                                   <  2:2933231/101‑1 (MQ=255)
                                                              ccGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCtt                                   >  1:8710865/1‑101 (MQ=255)
                                                               cGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTc                                  >  2:6833560/1‑101 (MQ=255)
                                                               cGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTc                                  <  1:3249321/101‑1 (MQ=255)
                                                               cGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTc                                  <  2:7945482/101‑1 (MQ=255)
                                                                gTTTGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCa                                 <  2:7137528/101‑1 (MQ=255)
                                                                gTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCa                                 >  2:9009636/1‑101 (MQ=255)
                                                                gTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCa                                 >  1:3310070/1‑101 (MQ=255)
                                                                 ttCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCAt                                <  2:2393587/101‑1 (MQ=255)
                                                                 ttCGGTCACCGAAATCCGGCCGATGTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCAt                                <  2:4958609/101‑1 (MQ=255)
                                                                  tCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATc                               <  2:2728112/101‑1 (MQ=255)
                                                                   cGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCg                              >  2:4018670/1‑101 (MQ=255)
                                                                   cGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCg                              >  1:3390860/1‑101 (MQ=255)
                                                                   cGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCg                              >  1:3131369/1‑101 (MQ=255)
                                                                    ggTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCgg                             >  2:4330503/1‑101 (MQ=255)
                                                                     gTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGc                            <  2:7811724/101‑1 (MQ=255)
                                                                       cACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGa                          <  2:5878739/101‑1 (MQ=255)
                                                                        aCCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGaa                         <  2:457352/101‑1 (MQ=255)
                                                                          cGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAAt                       >  1:5047825/1‑101 (MQ=255)
                                                                          cGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAAt                       >  2:7703801/1‑101 (MQ=255)
                                                                          cGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAAt                       <  2:7305517/101‑1 (MQ=255)
                                                                           gAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATc                      >  1:1536036/1‑101 (MQ=255)
                                                                           gAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGCCAGGCCTTCATCGGCGAAATc                      >  1:9156480/1‑101 (MQ=255)
                                                                            aaaTCCGGCCGATTTCCGCCGACAACTGCGCCGGAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCg                     >  1:4776138/1‑101 (MQ=255)
                                                                            aaaTCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCg                     >  2:5290630/1‑101 (MQ=255)
                                                                            aaaTCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCg                     >  1:93919/1‑101 (MQ=255)
                                                                              aTCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGAt                   <  1:585690/101‑1 (MQ=255)
                                                                                ccGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGc                 <  1:6286098/101‑1 (MQ=255)
                                                                                   gCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAgg              <  2:9183844/101‑1 (MQ=255)
                                                                                    ccGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGc             >  1:7287936/1‑101 (MQ=255)
                                                                                       aTTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTc          >  2:1365895/1‑101 (MQ=255)
                                                                                       aTTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTc          <  1:6905045/101‑1 (MQ=255)
                                                                                        tttCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTcc         <  2:1771186/101‑1 (MQ=255)
                                                                                         ttCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCa        <  2:6086420/101‑1 (MQ=255)
                                                                                         ttCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGCCGTCACATCAACCGCCAGGCCTTCATCGGGGAAATCGATGCCGGCCTccc        >  2:1309291/1‑100 (MQ=255)
                                                                                          tCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCAt       >  2:7597445/1‑101 (MQ=255)
                                                                                          tCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCAt       <  1:7427780/101‑1 (MQ=255)
                                                                                          tCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCAt       <  2:341547/101‑1 (MQ=255)
                                                                                           ccgccgACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATc      <  2:4140713/101‑1 (MQ=255)
                                                                                              ccgACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCAgc   >  1:1866768/1‑101 (MQ=255)
                                                                                               cgACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCAgcg  <  1:5329766/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AGAAGCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGAAAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCAGCG  >  NC_014034/73997‑74192

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG