Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 833,231 C→A 100% G35V (GGC→GTC)  mcpA1 ← methyl‑accepting chemotaxis protein McpA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034833,2310C→A100.0% 2130.1 555G35V (GGC→GTC) mcpA1methyl‑accepting chemotaxis protein McpA
Reads supporting (aligned to +/- strand):  new base (282/268):  ref base (1/3):  total (284/271)

Only 100 of 569 total aligned reads displayed.

TGCCCGCTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGCCCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGTT  >  NC_014034/833133‑833328
                                                                                                  |                                                                                                 
 gCCCGCTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCg                                                                                                >  2:4283715/1‑101 (MQ=255)
 gCCCGCTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCg                                                                                                >  2:4769833/1‑101 (MQ=255)
    cGCTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCaa                                                                                             <  2:3259824/101‑1 (MQ=255)
     gCTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCaaa                                                                                            <  1:7137830/101‑1 (MQ=255)
      cTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCaaaa                                                                                           <  1:4400203/101‑1 (MQ=255)
      cTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCaaaa                                                                                           >  1:3876524/1‑101 (MQ=255)
      cTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCaaaa                                                                                           >  2:5676728/1‑101 (MQ=255)
       ttGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAAc                                                                                          >  2:8467052/1‑101 (MQ=255)
        tGCACCTGTGCTTCCAGGTCGAGCAGCATTCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGGATCTCATAGGCGTTCGAGCCCGGAAAACg                                                                                         <  2:7545793/101‑1 (MQ=255)
         gCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGc                                                                                        <  2:8815290/101‑1 (MQ=255)
         gCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGc                                                                                        <  1:4984475/101‑1 (MQ=255)
            ccTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCAcc                                                                                     >  2:1163427/1‑101 (MQ=255)
            ccTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCAcc                                                                                     <  2:7407173/101‑1 (MQ=255)
             cTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCa                                                                                    <  2:7741860/101‑1 (MQ=255)
             cTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCa                                                                                    >  2:410936/1‑101 (MQ=255)
             cTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCa                                                                                    <  2:2426349/101‑1 (MQ=255)
             cTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCa                                                                                    >  1:7742193/1‑101 (MQ=255)
              tgtgCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGNANCan                                                                                   >  1:2348446/1‑100 (MQ=255)
              tgtgCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAg                                                                                   >  1:9198886/1‑101 (MQ=255)
               gtgCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGc                                                                                  <  2:5538754/101‑1 (MQ=255)
                 gCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGt                                                                                >  1:5019360/1‑101 (MQ=255)
                  cTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGtt                                                                               <  1:4104073/101‑1 (MQ=255)
                   ttCCAGATCGATCAGCATGTCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGttt                                                                              <  1:3500447/101‑1 (MQ=255)
                   ttCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGttt                                                                              <  1:8171859/101‑1 (MQ=255)
                     ccAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCg                                                                            <  1:5141642/101‑1 (MQ=255)
                     ccAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCg                                                                            <  1:2600732/101‑1 (MQ=255)
                       aGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGa                                                                          >  2:5242471/1‑101 (MQ=255)
                       aGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGa                                                                          >  1:5782691/1‑101 (MQ=255)
                           cgatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAg                                                                      <  2:2350401/101‑1 (MQ=255)
                           cgatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAg                                                                      >  2:3073879/1‑101 (MQ=255)
                            gatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAga                                                                     >  2:4508609/1‑101 (MQ=255)
                            gatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAga                                                                     >  2:6578559/1‑101 (MQ=255)
                              tcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAgatg                                                                   <  1:3194863/101‑1 (MQ=255)
                               cAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAgatga                                                                  <  1:3078727/101‑1 (MQ=255)
                               cAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAgatga                                                                  >  2:6425492/1‑101 (MQ=255)
                                aGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGGGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCACCGTTCCGGACAAgatgat                                                                 >  1:2982663/1‑101 (MQ=255)
                                  cATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGAtgatga                                                               <  2:6182643/101‑1 (MQ=255)
                                     gCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGTGCg                                                            <  2:384774/101‑1 (MQ=255)
                                      ccGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGa                                                           <  1:654957/101‑1 (MQ=255)
                                       cGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGaa                                                          <  2:7229672/101‑1 (MQ=255)
                                        gATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGaaa                                                         >  1:8437096/1‑101 (MQ=255)
                                         aTGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAc                                                        <  2:6977347/101‑1 (MQ=255)
                                         aTGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAc                                                        >  1:4498961/1‑101 (MQ=255)
                                          tGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAcg                                                       <  1:5937308/101‑1 (MQ=255)
                                           ggCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGTGTTTCGGACAAGATGATGAGCGAAAcgg                                                      >  1:6397814/1‑101 (MQ=255)
                                             cGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAcggcg                                                    >  1:7087335/1‑101 (MQ=255)
                                              ggTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAcggcgg                                                   >  1:6862183/1‑101 (MQ=255)
                                              ggTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAcggcgg                                                   >  1:157539/1‑101 (MQ=255)
                                                tgtCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCa                                                 >  1:1295455/1‑101 (MQ=255)
                                                 gtCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTGGGACAAGATGATGAGCGAAACGGCGGCaa                                                >  1:4808496/1‑101 (MQ=255)
                                                      tGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGcc                                           <  2:6736589/101‑1 (MQ=255)
                                                      tGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGcc                                           <  1:1099993/101‑1 (MQ=255)
                                                       gACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCa                                          <  2:6012716/101‑1 (MQ=255)
                                                       gACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCa                                          <  1:1688912/101‑1 (MQ=255)
                                                        aCGTTGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCAc                                         <  1:7353729/101‑1 (MQ=255)
                                                         cGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACg                                        <  2:8617578/101‑1 (MQ=255)
                                                         cGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACg                                        <  2:3122984/101‑1 (MQ=255)
                                                         cGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACg                                        >  1:7679731/1‑101 (MQ=255)
                                                            tgCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATc                                     <  2:145643/100‑1 (MQ=255)
                                                            cGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATc                                     >  2:1663218/1‑101 (MQ=255)
                                                             gCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCg                                    >  2:826155/1‑101 (MQ=255)
                                                              cTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGc                                   <  1:431104/101‑1 (MQ=255)
                                                                gAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCAt                                 <  1:5180487/101‑1 (MQ=255)
                                                                  ggttttttgcgcgCATCTCATAGGCGTTCGAGCCCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAg                               <  2:2942510/92‑1 (MQ=255)
                                                                  ggTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAg                               >  1:8267131/1‑101 (MQ=255)
                                                                   gtgtTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGa                              <  1:6078074/101‑1 (MQ=255)
                                                                     gttgttCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATg                            >  1:4572340/1‑101 (MQ=255)
                                                                     gttgttCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATg                            >  2:8025324/1‑101 (MQ=255)
                                                                     gttgttCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATg                            >  1:728034/1‑101 (MQ=255)
                                                                      ttgttCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATgc                           <  1:7496744/101‑1 (MQ=255)
                                                                         ttCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATgcggg                        >  2:4662483/1‑101 (MQ=255)
                                                                          tCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATgcgggc                       <  2:1295423/101‑1 (MQ=255)
                                                                             cgcATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGa                    >  2:6913219/1‑101 (MQ=255)
                                                                              gcATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGaa                   <  1:1894112/101‑1 (MQ=255)
                                                                               cATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGaaa                  >  1:554696/1‑101 (MQ=255)
                                                                               cATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGaaa                  >  1:8663604/1‑101 (MQ=255)
                                                                                aTCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAAt                 <  1:2764573/101‑1 (MQ=255)
                                                                                aTCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAAt                 <  2:2169253/101‑1 (MQ=255)
                                                                                aTCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAAt                 <  1:8749338/101‑1 (MQ=255)
                                                                                aTCTCATAGGCGCTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAAt                 <  1:6273235/101‑1 (MQ=255)
                                                                                 tctcATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATa                <  1:8924966/101‑1 (MQ=255)
                                                                                  ctcATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATAc               >  2:5828303/1‑101 (MQ=255)
                                                                                  ctcATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATAc               <  1:5358543/101‑1 (MQ=255)
                                                                                   tcATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATAcc              <  1:6710445/101‑1 (MQ=255)
                                                                                   tcATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATAcc              <  1:691957/101‑1 (MQ=255)
                                                                                    cATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCg             >  1:9071516/1‑101 (MQ=255)
                                                                                     aTAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCga            <  1:1627982/101‑1 (MQ=255)
                                                                                     aTAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCga            >  1:2139572/1‑101 (MQ=255)
                                                                                     aTAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCga            >  2:3898030/1‑101 (MQ=255)
                                                                                      tAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaa           <  2:3639010/101‑1 (MQ=255)
                                                                                      tAGGCGTGCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaa           <  1:2824126/101‑1 (MQ=255)
                                                                                          cGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAAt       >  1:6015483/1‑101 (MQ=255)
                                                                                           gTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGACAAACCGAAGAAtt      >  2:4033172/1‑101 (MQ=255)
                                                                                           gTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAAtt      <  2:5149798/101‑1 (MQ=255)
                                                                                            ttCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAAttt     >  2:2646193/1‑101 (MQ=255)
                                                                                            ttCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAAttt     <  2:2891943/101‑1 (MQ=255)
                                                                                             tCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTg    <  2:8188550/101‑1 (MQ=255)
                                                                                              cGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGt   <  2:4839390/101‑1 (MQ=255)
                                                                                               gAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGtt  <  2:9057541/101‑1 (MQ=255)
                                                                                               gAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGtt  >  2:5432452/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
TGCCCGCTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGCCCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGTT  >  NC_014034/833133‑833328

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG