Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	854,914	T→G	17.7%	intergenic (+103/‑45)	hypD → / → hypE	hydrogenase expression/formation protein HypD/hydrogenase expression/formation protein HypE

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	854,914	0	T→G	17.7%	3.4 ‑2.4 0.0	72	intergenic (+103/‑45)	hypD/hypE	hydrogenase expression/formation protein HypD/hydrogenase expression/formation protein HypE
Reads supporting (aligned to +/- strand):  new base (16/1):  ref base (40/15):  total (56/16)
Fisher's exact test strand distribution p-value = 9.50e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

CCCAAGCACGGCATCGCCTCCCCCCGGGCCCCCCACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGCGAT  >  NC_014034/854865‑855014                                                  |                                                                                                     ccggggggaggCATCGCCCCCCCCCGGGCCCCCCACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGt                                                   <  1:5256792/92‑1 (MQ=255)     tctgaggCATCGCCTCCCCCCGGGCCCCCCACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTgag                                               <  1:2486929/96‑1 (MQ=255)     ggggaggCATCGCCTCCCCCCGGGCCCCCCACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTgag                                               >  2:5967425/6‑101 (MQ=255)      gggaggCATCGCCTCCCCCCGGGCCCCCCACCGACCCGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTgaga                                              >  2:3558431/5‑101 (MQ=255)      ggcagccaccGCCCCCCCCCGGGCCCCCCACCGACCGGGGGAGGTGCGACCTCCCCCGGTCCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTgaga                                              <  1:7476422/92‑1 (MQ=255)       ggaggCATCGCCTCCCCCCGGGCCCCCCACCGACCGGGGGAGGTGGGAACTCCCCCCGCCACCCGCGACGCATTTCGAGCAAGAGGAGATGGCGTTgcgag                                             >  2:8256476/4‑101 (MQ=255)       ggaggCATCGCCTCCCCCCGGGCCCCCCACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTgagag                                             <  2:2802524/98‑1 (MQ=255)        gaggCGTCGCCTCCCGCCGGGCCCCCCACCGACCGGGGGAGGGGCGACCTCCCCCGGACCCCCGCGACGGATTTGGAGCCAGATGAGATGGCGTTgggaga                                            >  1:8849860/3‑101 (MQ=255)        gaggCATCGCCTCCCCCCGGGCCCCCCACCGACCGGGGGGGGGGCGACCTCCCCCGGACCCCCGCGACGGATTTGGAGCAAGATGAGATGGCGTTgggaga                                            >  2:2486856/3‑101 (MQ=255)          ggCATCGCCTCCCCCCGGGCCCCCCACCGACCGGGGGGGGGGAGACCTCCCCCGGACCCCCACCTCCTAGTTGGAGCAAGATTGGATGGGGGTgagggacg                                          >  2:4740741/1‑99 (MQ=255)          ggCATCGCCTCCCCCCGGGCCCCCCACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATg                                          <  1:446611/101‑1 (MQ=255)           gCATCGCCTCCCCCCGGGCCCCCCACCGACCGGGGGAGGGGCGACCTCCCCCGGACCCCCGCGACGGATTTGGAGCAAGATGAGATGGCGTTGAGAGATGa                                         >  2:5330446/1‑101 (MQ=255)             aTCGCCTCCCCCCGGGCCCCCCACCGACCGGGGGGGGGGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGGGTTGAGAGATGAGc                                       >  2:6479541/1‑101 (MQ=255)              tCGCCTCCCCCCGGGCCCCCCACCGACCGGGGGGGGGGCGGGCCCCCCCCGACCCCCCCGCCCCATTTTGGGGAAGAAGAGAGGGGGGTGGGGGAGGAGCg                                      >  1:781024/1‑101 (MQ=255)               cGCCTCCCCCCGGGCCCCCCACCGACCGGGGGGGGGGCGACCTCCCCCGGACCCCCCCGACGTATTTGGAGCAAGATGGGATGGCGTTGAGAGATGAGCgg                                     >  2:2489047/1‑101 (MQ=255)               cGCCTCCCCCCGGGCCCCCCACCGACCCGGGGGGGGGGGACCTCCCCCCGACCCCCCCCCCGCATTTTGAGCAAGATGAGATGGCGTGGAGAGGAGAGCgg                                     >  1:1314738/1‑101 (MQ=255)               cGCCTCCCCCCGGGCCCCCCACCGACCCGGGGAGGGGCGACCTCCCCCGGACCCCCGCGACGGATTTGGAGCAAGATGAGATGGCGGTGCGAGATGAGCgg                                     >  2:7107681/1‑101 (MQ=255)                gCCTCCCCCCGGGCCCCCCACCGACCCGGGGGGGTGGCCCCCCCCCCCGGCCCCCCCGCCGCCTTTGGAGCCAGACAAGAAGGCGGTGAGAGAAGAGCggg                                    >  2:8985101/1‑101 (MQ=255)                 ccTCCCCCCGGGCCCCCCACCGACCGGGGGAGGGGCGACCTCCCCCGGACCCCCGCGACGCATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCgggg                                   >  1:4266671/1‑100 (MQ=255)                  cTCCCCCCGGGCCCCCCACCGACCGGGGGGGGGGGGGCCCCCCCCCGCCCCCCCCGCCGCATTTGGAGCAAGATGAGATGGCGTTGGGAGATGGGGggggg                                  >  1:2209312/1‑99 (MQ=255)                  cTCCCCCCGGGCCCCCCACCGACCGGGGGGGGGGCGAACTCCCCCCGGCCCCCGCGACGCATTTGGGGCAAGATGGGGTGGGGGTGGGGGATGAGCgggcg                                  >  2:5302371/1‑99 (MQ=255)                  cTCCCCCCGGGCCCCCCACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTg                                  >  1:2985359/1‑101 (MQ=255)                   tCCCCCCGGGCCCCCCACCGACCGGGGGGGGTGCGACCTCCCCCGGACCCCCCCGACGTATTTGGAGCAAGATGAGAAGGGGGTGAGGGATGGGCgggggg                                 >  2:5896011/1‑98 (MQ=255)                    ccccccGGGCCCCCCACCGACCGGGGGAGGTGCGACCTCCCCCGGTCCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGAc                                <  1:5433183/101‑1 (MQ=255)                      ccccGGGCCCCCCACCGACCCGGGGGGGGGCGACCTCCCCCCGACCCCCCCGCCGGATTTTGGGGAAGATGAGATGGGGGTGGGGGGATAGCGGGGGACGc                              >  2:5004193/1‑101 (MQ=255)                        ccGGGCCCCCCACCGACCGGGGGAGGGGCGAGCTCCCCCCGGCCCCCACGGCCCACTTGGAGCAAGAAGAAATGGCGTGTAGAGATAGACGGGTGGCGCTg                            >  2:484905/1‑101 (MQ=255)                          gggCCCCCCACCGACCGGGGGAGGGGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGc                          <  2:1071285/101‑1 (MQ=255)                          gggCCCCCCACCGACCGGGGGAGGGGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGc                          >  2:4765552/1‑101 (MQ=255)                          gggCCCCCCACCGACCGGGGGAGGGGCGACCTCCCCCGGACCCCCCCGACGTATTTGGGGCAAGATGAGANNNNNTTGAGAGATGAGCGGGGGGCGCCGGc                          >  1:5376191/1‑101 (MQ=255)                          gggCCCCCCACCGACCCGGGGGGGGGGGGCCCCCCCCCGGCCCCCCCGGCGCATTTTGGGGAAGAAGAGATGGGGGTGAGAGATGGGCGGGGGGAGCTGGc                          >  2:2160372/1‑101 (MQ=255)                           ggCCCCCCACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGca                         >  2:5643779/1‑101 (MQ=255)                           ggCCCCCCACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGAGGGGGTTGAGAGGTGGGCGGGGGAGGCTGGca                         >  2:4273297/1‑101 (MQ=255)                           ggCCCCCCACCGACCGGGGGAGGGGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGca                         >  2:7460780/1‑101 (MQ=255)                           ggCCCCCCACCGACCGGGGGAGGGGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGGTGAGAGATGAGCGGGGGACGCTGGca                         >  2:6504577/1‑101 (MQ=255)                            gCCCCCCACCGACC.GGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGcaca                       >  1:8736520/1‑101 (MQ=255)                             ccccccACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGcaca                       <  1:5896246/101‑1 (MQ=255)                                cccACCGACCGGGGGGGGGGGGGCCCCCCCCCGGCCCCCCCGCCGGATTTGGGGGCAGATGAGAAGGGGGTGAGAGAGGAGCGGGGGGCGCTggggcaagg                    >  2:4431453/1‑94 (MQ=255)                                cccACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATgg                    >  2:6459748/1‑101 (MQ=255)                                cccACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATgg                    <  1:4037519/101‑1 (MQ=255)                                 ccACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATggc                   >  2:3799100/1‑101 (MQ=255)                                 ccACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATggc                   >  2:2905178/1‑101 (MQ=255)                                 ccACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATggc                   >  2:2299768/1‑101 (MQ=255)                                  cgccgaccgGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCGCATggcg                  <  2:55025/99‑1 (MQ=255)                                  cACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATggcg                  >  2:5068465/1‑101 (MQ=255)                                    ccgaccgGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATggcggc                >  2:2299337/1‑101 (MQ=255)                                    ccgaccgGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATggcggc                >  2:2829540/1‑101 (MQ=255)                                    ccgaccgGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATggcggc                <  1:5330630/101‑1 (MQ=255)                                      gaccgGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGcggcgg              >  2:1275482/1‑101 (MQ=255)                                      gaccgGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGGGACGCTGGCACATGGcggcgg              >  1:931571/1‑101 (MQ=255)                                       ctcgGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGCGATGAGCGGGTGACGCTGGCACATGGCGGCGGt             <  1:6447425/99‑1 (MQ=255)                                       accgGGGGGGGGGCGACCCCCCCCCGGCCCCCCCGGCGTATTTGGAGCAAGATGAGATGGCGGTGAGAGGTGAGCGGGGGACGCCGGCACATGGcgggggg             >  2:6177431/1‑100 (MQ=255)                                       accgGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGcggcggg             >  2:1895949/1‑100 (MQ=255)                                       accgGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGcggcggg             >  1:4991630/1‑100 (MQ=255)                                         cgGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGGGACGCTGGCACATGGCGGCGGGgg           >  2:5798161/1‑101 (MQ=255)                                         cgGGGGAGGGGCGACCTCCCCCGGACCCCCGCGCCGTATTTGGAGCAAAATGAGATGGCGTTGAGAGATGAGCGGGAGGCGCTGGCACATGGCGGCGGTgg           >  2:7433415/1‑101 (MQ=255)                                          gggggAGGTGCGACCTCCCCCGGACCCCCGCGAGGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGc          >  1:6443475/1‑101 (MQ=255)                                          gggggAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGc          >  1:6342440/1‑101 (MQ=255)                                          gggggAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGc          >  1:5736486/1‑101 (MQ=255)                                          gggggAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGc          >  2:6928411/1‑101 (MQ=255)                                           ggggAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCa         >  1:2499930/1‑101 (MQ=255)                                           ggggAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCa         >  1:6432922/1‑101 (MQ=255)                                           ggggAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCa         >  1:3784305/1‑101 (MQ=255)                                            gggAGTTGCTCCCTCCCCCGCCCCCCCGCGACGTATTTGGAGCAAGATTAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCaa        <  1:8932332/101‑1 (MQ=255)                                            gggAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCaa        >  1:8688536/1‑101 (MQ=255)                                             ggAGGTGCGACCTCCCCCGGACCGCCGCGGCGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCaaa       <  1:7671533/101‑1 (MQ=255)                                             ggAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCaaa       >  2:5124500/1‑101 (MQ=255)                                              gAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGTGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAg      <  2:3062528/101‑1 (MQ=255)                                              gAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAg      <  1:2022460/101‑1 (MQ=255)                                              gAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAg      <  2:8849454/101‑1 (MQ=255)                                              gAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAg      >  1:2059218/1‑101 (MQ=255)                                              gAGGTGCGACCTCCCACGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAg      <  1:8564736/101‑1 (MQ=255)                                              gAGGTCCGACCTCCCCCGGGCCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAg      <  1:790338/101‑1 (MQ=255)                                               aGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGTACATGGAGACGGAGGCaagac     >  1:5396348/1‑98 (MQ=255)                                               aGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGc     >  1:2827748/1‑101 (MQ=255)                                               aGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGc     >  2:4277828/1‑101 (MQ=255)                                               aGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGc     >  1:407389/1‑101 (MQ=255)                                               aGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGc     >  2:7726742/1‑101 (MQ=255)                                                ggTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCGAAGCg    >  2:6218032/1‑101 (MQ=255)                                                ggTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGCg    >  2:3258235/1‑101 (MQ=255)                                                ggTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGCg    >  2:239193/1‑101 (MQ=255)                                                ggTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGCg    >  2:7725512/1‑101 (MQ=255)                                                 gTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGCGa   >  1:3489801/1‑101 (MQ=255)                                                 gTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGCGa   >  2:3243830/1‑101 (MQ=255)                                                 gTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGCGa   >  1:7059547/1‑101 (MQ=255)                                                  tGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGCGAt  >  2:5955751/1‑101 (MQ=255)                                                  tGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGCGAt  >  2:8656707/1‑101 (MQ=255)                                                  |                                                                                                     CCCAAGCACGGCATCGCCTCCCCCCGGGCCCCCCACCGACCGGGGGAGGTGCGACCTCCCCCGGACCCCCGCGACGTATTTGGAGCAAGATGAGATGGCGTTGAGAGATGAGCGGGTGACGCTGGCACATGGCGGCGGTGGCAAAGCGAT  >  NC_014034/854865‑855014 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG