Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 861,642 G→A 100% A809T (GCC→ACC)  RCAP_rcc00783 → diguanylate cyclase/phosphodiesterase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034861,6420G→A100.0% 1325.2 363A809T (GCC→ACC) RCAP_rcc00783diguanylate cyclase/phosphodiesterase
Reads supporting (aligned to +/- strand):  new base (159/204):  ref base (0/0):  total (159/204)

Only 100 of 391 total aligned reads displayed.

TGACCGAGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCGCCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCGAGGCGGACCGCAACGC  >  NC_014034/861544‑861739
                                                                                                  |                                                                                                 
tGACCGAGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCAcc                                                                                                 >  1:6165769/1‑101 (MQ=255)
tGACCGAGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCAcc                                                                                                 <  1:769955/101‑1 (MQ=255)
tGACCGAGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCAcc                                                                                                 >  1:3657995/1‑101 (MQ=255)
tGACCGAGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCAcc                                                                                                 <  2:370298/101‑1 (MQ=255)
 gACCGAGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCg                                                                                                >  2:8785144/1‑101 (MQ=255)
 gACCGAGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCg                                                                                                <  2:3294663/101‑1 (MQ=255)
  aCCGAGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCgg                                                                                               >  2:684851/1‑101 (MQ=255)
  aCCGAGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCgg                                                                                               <  1:8916344/101‑1 (MQ=255)
    cGAGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCt                                                                                             >  2:5441771/1‑101 (MQ=255)
    cGAGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCt                                                                                             <  2:5052613/101‑1 (MQ=255)
     gcggATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTa                                                                                            <  2:4764558/99‑1 (MQ=255)
     gAGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTa                                                                                            <  1:3098792/101‑1 (MQ=255)
      aGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTAt                                                                                           <  1:4946752/101‑1 (MQ=255)
      aGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTAt                                                                                           <  2:2117888/101‑1 (MQ=255)
       ggATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTAtt                                                                                          >  1:752680/1‑101 (MQ=255)
         aTCAGCTGGCCGCCCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGCCTGCGGCTGGGGATCGACGATTTTGGCACCGGTCATTcc                                                                                        >  2:1306652/1‑101 (MQ=255)
            aGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGGTCGGGGATTTCGGCACCGGCTATTCCTGt                                                                                     >  1:7338132/1‑101 (MQ=255)
              cTGGCCGACCGGGTAGCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCt                                                                                   <  2:1947123/101‑1 (MQ=255)
               tGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTg                                                                                  <  2:4865201/101‑1 (MQ=255)
                ggCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGGCGATTTGGGCCCCGGCTTCTCCTCTcggc                                                                                 >  2:5694736/1‑98 (MQ=255)
                ggCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTgg                                                                                 >  2:7419773/1‑101 (MQ=255)
                 gCCGAGCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGc                                                                                <  2:726003/101‑1 (MQ=255)
                  ccGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGcc                                                                               <  1:7927207/101‑1 (MQ=255)
                   cgtccGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCt                                                                              <  2:9054999/98‑1 (MQ=255)
                   cggccGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCt                                                                              <  1:4456896/98‑1 (MQ=255)
                    gACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTa                                                                             >  1:9001322/1‑101 (MQ=255)
                     aCCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTAt                                                                            >  1:5815319/1‑101 (MQ=255)
                     aCCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTAt                                                                            <  2:6036936/101‑1 (MQ=255)
                      ccGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATc                                                                           <  2:1849468/101‑1 (MQ=255)
                       cGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCt                                                                          <  1:1916977/101‑1 (MQ=255)
                        gggTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTg                                                                         <  2:4433817/101‑1 (MQ=255)
                         ggTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTgg                                                                        <  1:4641781/101‑1 (MQ=255)
                          gTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGgc                                                                       >  2:6211978/1‑101 (MQ=255)
                          gTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGgc                                                                       <  1:2248402/101‑1 (MQ=255)
                          gTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGgc                                                                       <  2:3471610/101‑1 (MQ=255)
                          gTTCCCGGCGCGGTCGGGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGgc                                                                       <  1:2977001/101‑1 (MQ=255)
                            tCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGgcgc                                                                     <  1:6839567/101‑1 (MQ=255)
                               gggCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGc                                                                  >  1:571475/1‑101 (MQ=255)
                                ggCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCt                                                                 <  1:7420097/101‑1 (MQ=255)
                                   gcgGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGcc                                                              <  2:7934955/101‑1 (MQ=255)
                                   gcgGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGcc                                                              <  1:3861420/101‑1 (MQ=255)
                                    cgGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCg                                                             <  2:262983/101‑1 (MQ=255)
                                    cgGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCg                                                             <  1:6703177/101‑1 (MQ=255)
                                     ggTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCgg                                                            <  2:8918973/101‑1 (MQ=255)
                                      gTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGt                                                           >  2:8585411/1‑101 (MQ=255)
                                        cggggCGTTGGGCCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGTTTTGGGCACCGGCTATTCCTGTCTGGCCTTTCGGGCGCGGCTGCCGGtct                                                         <  2:3069576/98‑1 (MQ=255)
                                        cGAGGCGCTTGGGCGGGTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGtct                                                         <  1:1125398/101‑1 (MQ=255)
                                         gAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGtctc                                                        <  1:2794450/101‑1 (MQ=255)
                                         gAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGtctc                                                        >  1:2166826/1‑101 (MQ=255)
                                          aGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCg                                                       <  2:4170092/101‑1 (MQ=255)
                                          aGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCg                                                       >  1:6633312/1‑101 (MQ=255)
                                          aGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCCCg                                                       >  1:4711431/1‑101 (MQ=255)
                                            gcgcTGGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGAt                                                     <  1:324649/101‑1 (MQ=255)
                                               cTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCt                                                  >  1:6367040/1‑101 (MQ=255)
                                               cTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCt                                                  <  1:6088956/101‑1 (MQ=255)
                                               cTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCt                                                  >  2:8205766/1‑101 (MQ=255)
                                               cTTGGGCGGTCGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCt                                                  <  1:8098337/101‑1 (MQ=255)
                                                ttGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTg                                                 >  2:7369970/1‑101 (MQ=255)
                                                 tGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGa                                                <  1:1336770/101‑1 (MQ=255)
                                                 tGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGa                                                >  1:4844517/1‑101 (MQ=255)
                                                 tGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGCTCTCGATGCTGa                                                <  1:6541265/101‑1 (MQ=255)
                                                  gggCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGaa                                               >  2:6122751/1‑101 (MQ=255)
                                                    gCGGTTGCGCGCGCGCGGGGTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGa                                             <  2:2260812/101‑1 (MQ=255)
                                                     cGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGAt                                            <  1:3444892/101‑1 (MQ=255)
                                                      ggTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATc                                           >  1:3531487/1‑101 (MQ=255)
                                                       gTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCg                                          >  1:3174519/1‑101 (MQ=255)
                                                       gTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCg                                          >  2:5823032/1‑101 (MQ=255)
                                                        ttGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGa                                         <  1:255198/101‑1 (MQ=255)
                                                         tGCGCGCGCGCGGGCTGGGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGAc                                        <  1:7040210/101‑1 (MQ=255)
                                                          gcgcgcgcgcGGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGAcg                                       <  1:4254738/101‑1 (MQ=255)
                                                           cgcgcgcgcgGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGAcgc                                      <  2:7821300/101‑1 (MQ=255)
                                                             cgcgcgcgGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACgcgc                                    <  1:1477310/101‑1 (MQ=255)
                                                              gcgcgcgGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCa                                   >  2:6707493/1‑101 (MQ=255)
                                                               gtgtcgGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGGTGCTGGAGATGCACGCGCAt                                  >  2:3409513/5‑101 (MQ=25)
                                                                gcgcgGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCAtt                                 <  2:3985228/101‑1 (MQ=255)
                                                                gcgcgGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCAtt                                 >  1:8625339/1‑101 (MQ=255)
                                                                  gcgGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTc                               <  1:4576077/101‑1 (MQ=255)
                                                                   cgGGCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCg                              <  1:8759091/101‑1 (MQ=255)
                                                                    gggCTGCGGCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGt                             >  2:1125138/1‑101 (MQ=255)
                                                                        tGCGGCTGGCGATGGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTgcgc                         <  2:1604151/101‑1 (MQ=255)
                                                                          gggCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCgg                       <  2:4122680/100‑1 (MQ=255)
                                                                           ggCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGc                      <  2:2466250/101‑1 (MQ=255)
                                                                           ggCTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGc                      >  1:8358238/1‑101 (MQ=255)
                                                                             cTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCAt                    <  1:6279936/101‑1 (MQ=255)
                                                                             cTGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCAt                    <  1:4625494/101‑1 (MQ=255)
                                                                              tGGCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGGGCATTGCGTGCGCGGCATc                   >  1:1002044/1‑101 (MQ=255)
                                                                              gggCGAGCGACGCTTCCCGCCCCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATc                   <  2:5522308/100‑1 (MQ=25)
                                                                               ggCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCg                  <  1:1731777/101‑1 (MQ=255)
                                                                               ggCGATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCg                  <  1:4054486/101‑1 (MQ=255)
                                                                                  gATCGACGATTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCGAgg               >  1:5308927/1‑101 (MQ=255)
                                                                                     cgacgaTTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCGAGGCgg            <  2:7721469/101‑1 (MQ=255)
                                                                                     cgacgaTTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCGAGGCgg            <  2:662022/101‑1 (MQ=255)
                                                                                       acgaTTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCGAGGCGGAc          >  2:3539962/1‑101 (MQ=255)
                                                                                       acgaTTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCGAGGCGGAc          <  2:2028110/101‑1 (MQ=255)
                                                                                        cgaTTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCGAGGCGGAcc         >  2:1766877/1‑101 (MQ=255)
                                                                                        cgaTTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTCCGCGGCATCGAGGCGGAcc         >  2:6400889/1‑101 (MQ=255)
                                                                                          aTTTCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCGAGGCgagatcg       >  2:277469/1‑95 (MQ=255)
                                                                                           tttCGGCACGGGCTATTCCGGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCGAGGCGGACCGCa      <  2:8019478/101‑1 (MQ=255)
                                                                                             tCGGCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCGAGGCGGACCGCAAc    <  1:5441956/101‑1 (MQ=255)
                                                                                               ggCACCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCGAGGCGGACCGCAACGc  >  1:7763465/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
TGACCGAGGATCAGCTGGCCGACCGGGTTCCGGGCGCGGTCGAGGCGCTTGGGCGGTTGCGCGCGCGCGGGCTGCGGCTGGCGATCGACGATTTCGGCGCCGGCTATTCCTGTCTGGCCTATCTGGCGCGGCTGCCGGTCTCGATGCTGAAGATCGACGCGCATTTCGTGCGCGGCATCGAGGCGGACCGCAACGC  >  NC_014034/861544‑861739

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG