Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,802,243 2 bp→GC 100% coding (626‑627/2124 nt) ligA → DNA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,802,2430C→G100.0% 1399.9 391T209R (ACG→AGG) ligADNA ligase
Reads supporting (aligned to +/- strand):  new base (182/209):  ref base (0/0):  total (182/209)
*NC_0140341,802,2440G→C100.0% 1424.9 391T209T (ACG→ACCligADNA ligase
Reads supporting (aligned to +/- strand):  new base (182/208):  ref base (0/0):  total (182/209)

Only 100 of 401 total aligned reads displayed.

GGCGCCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCT  >  NC_014034/1802148‑1802338
                                                                                               ||                                                                                              
  cgcCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGcc                                                                                          <  1:8735194/101‑1 (MQ=255)
  cgcCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGcc                                                                                          <  1:3166997/101‑1 (MQ=255)
   gcgtGCCGTGCGTGATGTCCGCGTCGAGGTCTACATGAGCCATGCCGATTTTGCTGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCa                                                                                         <  2:1593615/98‑1 (MQ=255)
    ccTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCaa                                                                                        >  1:6102770/1‑101 (MQ=255)
      tGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATc                                                                                      >  2:7761776/1‑101 (MQ=255)
      tGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATc                                                                                      >  1:1656385/1‑101 (MQ=255)
        ccGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCcg                                                                                    <  2:8466058/101‑1 (MQ=255)
          gTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCGATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCcgcg                                                                                  >  1:6453579/1‑101 (MQ=255)
             cTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCaa                                                                               <  1:4216983/101‑1 (MQ=255)
              tGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAAt                                                                              <  2:3512887/101‑1 (MQ=255)
              tGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAAt                                                                              <  2:4485461/101‑1 (MQ=255)
              tGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAAt                                                                              >  2:7592539/1‑101 (MQ=255)
               ggAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATg                                                                             >  2:1040031/1‑101 (MQ=255)
                gAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgc                                                                            <  1:3741988/101‑1 (MQ=255)
                 aaGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgcc                                                                           <  2:497124/101‑1 (MQ=255)
                  aGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgccg                                                                          >  2:1682044/1‑101 (MQ=255)
                  aGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgccg                                                                          <  2:545396/101‑1 (MQ=255)
                     ccGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGccgccg                                                                       >  2:6606743/1‑101 (MQ=255)
                      cgcgGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgccgcccc                                                                      >  2:5582436/1‑99 (MQ=255)
                        cggcgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGGGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCgg                                                                    >  2:1111090/1‑101 (MQ=255)
                        cggcgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCgg                                                                    >  2:3947960/1‑101 (MQ=255)
                         ggcgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCggg                                                                   >  1:51761/1‑101 (MQ=255)
                           cgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCt                                                                 >  1:5064172/1‑101 (MQ=255)
                            gAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTc                                                                >  1:2155201/1‑101 (MQ=255)
                             aGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcg                                                               >  1:4080338/1‑100 (MQ=255)
                              ggTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgc                                                              >  1:8405566/1‑99 (MQ=255)
                              ggTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCCACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgc                                                              <  2:4360328/101‑3 (MQ=255)
                                tCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgctg                                                            >  2:8356975/1‑97 (MQ=255)
                                tCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgctg                                                            >  1:8598950/1‑97 (MQ=255)
                                 cTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgctgc                                                           >  2:1814623/1‑96 (MQ=255)
                                 cTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgctgc                                                           <  1:1811319/101‑6 (MQ=255)
                                   aCATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcgc                                                         >  2:215031/1‑101 (MQ=255)
                                    cATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGcc                                                        <  2:9066130/101‑1 (MQ=255)
                                     aTGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCa                                                       <  2:2432235/101‑1 (MQ=255)
                                       gAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGc                                                     >  2:5875422/1‑101 (MQ=255)
                                       gAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGc                                                     >  1:1932405/1‑101 (MQ=255)
                                         gCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTc                                                   >  2:5061657/1‑101 (MQ=255)
                                          ccATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGTTGCGCCAGCTCg                                                  <  2:7289860/101‑1 (MQ=255)
                                           cATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCgc                                                 >  1:618481/1‑100 (MQ=255)
                                           cATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGa                                                 >  1:2317055/1‑101 (MQ=255)
                                            aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGAc                                                <  1:1068848/101‑1 (MQ=255)
                                            aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGAc                                                >  1:6949386/1‑101 (MQ=255)
                                              gCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGc                                              <  1:816495/101‑1 (MQ=255)
                                               ccGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGCCGcc                                             >  2:7369709/1‑101 (MQ=255)
                                                cGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGccc                                            <  2:7010726/101‑1 (MQ=255)
                                                 gATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCg                                           >  1:7664856/1‑101 (MQ=255)
                                                 gATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCg                                           >  1:7483810/1‑101 (MQ=255)
                                                 gATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCg                                           >  1:7191950/1‑101 (MQ=255)
                                                    tttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGAt                                        >  2:4735437/1‑101 (MQ=255)
                                                      tGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCa                                      <  2:2708755/101‑1 (MQ=255)
                                                       gcggcgCTGAATGCGCGGCAGGCGGAGCGTGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAc                                     <  2:1900528/101‑1 (MQ=255)
                                                       gcggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAc                                     <  2:7547474/101‑1 (MQ=255)
                                                        cggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAcc                                    <  2:51761/101‑1 (MQ=255)
                                                        cggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAcc                                    >  1:221714/1‑101 (MQ=255)
                                                        cggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAcc                                    >  1:4401464/1‑101 (MQ=255)
                                                          gcgcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGc                                  >  2:5251026/1‑101 (MQ=255)
                                                           cgcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGcc                                 >  2:9171466/1‑101 (MQ=255)
                                                           cgcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGcc                                 <  1:8324215/101‑1 (MQ=255)
                                                              tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                              <  2:8751879/101‑1 (MQ=255)
                                                              tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                              >  2:6487358/1‑101 (MQ=255)
                                                              tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                              <  1:3290710/101‑1 (MQ=255)
                                                              tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                              <  2:4131181/101‑1 (MQ=255)
                                                               gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                             <  1:1111108/101‑1 (MQ=255)
                                                                aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                            <  2:8233216/101‑1 (MQ=255)
                                                                aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                            <  1:2783128/101‑1 (MQ=255)
                                                                 aTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGa                           >  1:547482/1‑101 (MQ=255)
                                                                  tGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAc                          <  1:5789835/101‑1 (MQ=255)
                                                                   gcgcGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAcc                         <  1:7883672/101‑1 (MQ=255)
                                                                   gcgcGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAcc                         >  1:4155848/1‑101 (MQ=255)
                                                                   gcgcGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAcc                         >  1:5935048/1‑101 (MQ=255)
                                                                   gcgcGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAcc                         <  2:3768005/101‑1 (MQ=255)
                                                                    cgcgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAccc                        <  1:4701073/101‑1 (MQ=255)
                                                                      cgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCtt                      <  1:3078655/101‑1 (MQ=255)
                                                                      cgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCtt                      <  2:6199061/101‑1 (MQ=255)
                                                                      cgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCtt                      >  2:6295444/1‑101 (MQ=255)
                                                                      cgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCtt                      <  1:1439994/101‑1 (MQ=255)
                                                                       ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                     <  1:3643103/101‑1 (MQ=255)
                                                                       ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                     <  2:2812621/101‑1 (MQ=255)
                                                                       ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                     <  1:1581354/101‑1 (MQ=255)
                                                                       ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                     <  1:8250242/101‑1 (MQ=255)
                                                                         cAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaa                   <  2:3017494/101‑1 (MQ=255)
                                                                         cAGGCGGAGCGGAGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCTGACCCTTGaa                   >  1:6546815/1‑101 (MQ=255)
                                                                          aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                  <  2:3709267/101‑1 (MQ=255)
                                                                          aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                  >  2:7914710/1‑101 (MQ=255)
                                                                           ggCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAt                 >  1:2520973/1‑101 (MQ=255)
                                                                           ggCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAt                 <  1:8980259/101‑1 (MQ=255)
                                                                            gCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGACATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCTGGATCACCGCCTCCAGACCCTTGAAAtt                <  1:8085503/101‑1 (MQ=255)
                                                                            gCGGAGCGGGGCGACAAGAGCTTCGCCAATCCACGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAtt                <  2:6763828/101‑1 (MQ=255)
                                                                             cGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTc               <  1:7346479/101‑1 (MQ=255)
                                                                              ggAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCt              >  2:1338386/1‑101 (MQ=255)
                                                                              ggAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCt              >  2:150049/1‑101 (MQ=255)
                                                                                aGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCttt            <  2:6824080/101‑1 (MQ=255)
                                                                                aGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCttt            <  2:328479/101‑1 (MQ=255)
                                                                                aGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCttt            >  1:1261251/1‑101 (MQ=255)
                                                                                 gCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTg           >  1:6787810/1‑101 (MQ=255)
                                                                                  cGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGc          >  1:4868782/1‑101 (MQ=255)
                                                                                    gggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCt        >  1:9135761/1‑101 (MQ=255)
                                                                                       cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt     >  1:2621473/1‑101 (MQ=255)
                                                                                        gACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTc    <  2:6453330/101‑1 (MQ=255)
                                                                                         aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGACATTCTTTGCCTATGcc   >  2:3102312/1‑101 (MQ=255)
                                                                                               ||                                                                                              
GGCGCCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCT  >  NC_014034/1802148‑1802338

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG