Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,802,277 2 bp→GC 100% coding (660‑661/2124 nt) ligA → DNA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,802,2770C→G100.0% 1413.6 384S220S (TCC→TCGligADNA ligase
Reads supporting (aligned to +/- strand):  new base (158/226):  ref base (0/0):  total (158/226)
*NC_0140341,802,2780G→C100.0% 1418.4 384M221M (GTG→CTG) ligADNA ligase
Reads supporting (aligned to +/- strand):  new base (158/225):  ref base (0/0):  total (158/226)

Only 100 of 400 total aligned reads displayed.

ACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACGCAAT  >  NC_014034/1802183‑1802374
                                                                                              ||                                                                                                
aCATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcgc                                                                                             >  2:8237218/1‑101 (MQ=255)
 cATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGcc                                                                                            <  2:9066130/101‑1 (MQ=255)
  aTGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCa                                                                                           >  1:4980886/1‑101 (MQ=255)
   tGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAg                                                                                          >  2:6479384/1‑101 (MQ=255)
    gAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGc                                                                                         >  2:5875422/1‑101 (MQ=255)
     aGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTGCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCt                                                                                        >  2:5266815/1‑101 (MQ=255)
       ccATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCg                                                                                      <  2:4221407/101‑1 (MQ=255)
       ccATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCg                                                                                      <  2:1648836/101‑1 (MQ=255)
         aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGAc                                                                                    >  1:6949386/1‑101 (MQ=255)
           gCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGc                                                                                  <  1:816495/101‑1 (MQ=255)
           gCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGc                                                                                  >  2:1865386/1‑101 (MQ=255)
            ccGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGCCGcc                                                                                 >  2:7369709/1‑101 (MQ=255)
            ccGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGcc                                                                                 <  1:6122046/101‑1 (MQ=255)
             cGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGccc                                                                                <  2:3771367/101‑1 (MQ=255)
             cGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGccc                                                                                <  2:4980726/101‑1 (MQ=255)
             cGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGAGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGccc                                                                                <  1:5498748/101‑1 (MQ=255)
               aTTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCgg                                                                              <  1:3709011/101‑1 (MQ=255)
                ttttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGa                                                                             >  1:414208/1‑101 (MQ=255)
                 tttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGAt                                                                            >  2:758437/1‑101 (MQ=255)
                  ttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATc                                                                           <  1:2332806/101‑1 (MQ=255)
                  ttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATc                                                                           <  1:6017173/101‑1 (MQ=255)
                   tGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGGCAGGTCGGCGCCCGGAACa                                                                          >  2:5530658/1‑101 (MQ=255)
                   tGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCa                                                                          >  1:3374764/1‑101 (MQ=255)
                     cggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCGGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAcc                                                                        <  1:96132/101‑1 (MQ=255)
                     cggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAcc                                                                        <  2:51761/101‑1 (MQ=255)
                        cgcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGcc                                                                     >  2:9171466/1‑101 (MQ=255)
                        cgcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGcc                                                                     >  1:8249639/1‑101 (MQ=255)
                        cgcTGAATGCCCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGcc                                                                     <  1:2220047/101‑1 (MQ=255)
                           tGAATTCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCTTGTGCTCGCTGTGGCAGCTGGTTCTCCGGATTACGGCCTcc                                                                  >  2:653207/1‑101 (MQ=255)
                           tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                                                                  <  2:8751879/101‑1 (MQ=255)
                            gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                                                                 <  1:8368380/101‑1 (MQ=255)
                            gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                                                                 <  1:3239052/101‑1 (MQ=255)
                             aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                                                                <  2:6183322/101‑1 (MQ=255)
                             aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                                                                <  1:7570277/101‑1 (MQ=255)
                              aTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGa                                                               <  1:4704097/101‑1 (MQ=255)
                               tGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAc                                                              <  1:5789835/101‑1 (MQ=255)
                                gcgcGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAcc                                                             <  1:8087971/101‑1 (MQ=255)
                                   cgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCtt                                                          >  2:6295444/1‑101 (MQ=255)
                                   cgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCtt                                                          <  1:3078655/101‑1 (MQ=255)
                                   cgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCtt                                                          >  2:3099171/1‑101 (MQ=255)
                                   cgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCtt                                                          >  2:2057054/1‑101 (MQ=255)
                                    ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                                                         <  1:1581354/101‑1 (MQ=255)
                                    ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                                                         <  1:850157/101‑1 (MQ=255)
                                    ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                                                         >  1:453893/1‑101 (MQ=255)
                                      cAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaa                                                       <  1:4185709/101‑1 (MQ=255)
                                      cAGGCGGAGCGGAGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCTGACCCTTGaa                                                       >  1:6546815/1‑101 (MQ=255)
                                       aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                                                      <  2:3709267/101‑1 (MQ=255)
                                       aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                                                      <  2:6668411/101‑1 (MQ=255)
                                        ggCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAt                                                     <  2:1111778/101‑1 (MQ=255)
                                         gCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAtt                                                    <  1:1884556/101‑1 (MQ=255)
                                         gCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAtt                                                    <  1:258459/101‑1 (MQ=255)
                                          cGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTc                                                   <  2:6949101/101‑1 (MQ=255)
                                           ggAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCt                                                  >  2:150049/1‑101 (MQ=255)
                                            gAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCtt                                                 <  2:7743673/101‑1 (MQ=255)
                                             aGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCttt                                                <  2:6824080/101‑1 (MQ=255)
                                             aGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCttt                                                >  1:5307244/1‑101 (MQ=255)
                                              gCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTg                                               >  1:6787810/1‑101 (MQ=255)
                                               cGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGc                                              >  1:4868782/1‑101 (MQ=255)
                                                 gggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCt                                            <  1:7087191/101‑1 (MQ=255)
                                                  ggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTa                                           <  2:239055/101‑1 (MQ=255)
                                                   gCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAt                                          <  2:5718864/101‑1 (MQ=255)
                                                    cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt                                         <  1:8036941/101‑1 (MQ=255)
                                                     gACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTc                                        <  1:7370033/101‑1 (MQ=255)
                                                      aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTcc                                       <  2:2529842/101‑1 (MQ=255)
                                                      aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTcc                                       <  1:8314938/101‑1 (MQ=255)
                                                      aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTcc                                       <  1:7762109/101‑1 (MQ=255)
                                                       cAAGAGCTTCGCTAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCt                                      <  1:1640301/101‑1 (MQ=255)
                                                       cAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCt                                      <  1:215034/101‑1 (MQ=255)
                                                       cAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCt                                      <  2:7534529/101‑1 (MQ=255)
                                                        aagagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTg                                     <  2:3374660/95‑1 (MQ=255)
                                                         agagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTgg                                    <  2:2700654/96‑1 (MQ=255)
                                                         agagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTgg                                    >  2:4410819/6‑101 (MQ=255)
                                                         agagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTgg                                    <  2:2940463/96‑1 (MQ=255)
                                                          gagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTggg                                   >  2:4743858/5‑101 (MQ=255)
                                                              ttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGt                               <  2:400485/101‑1 (MQ=255)
                                                               tCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGtt                              <  2:2152913/101‑1 (MQ=255)
                                                               tCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCGGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGtt                              >  1:6436385/1‑101 (MQ=255)
                                                                 gCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCt                            >  1:2684008/1‑101 (MQ=255)
                                                                  ccAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTg                           <  2:4593414/101‑1 (MQ=255)
                                                                  ccAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTg                           <  1:1303148/101‑1 (MQ=255)
                                                                   cAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGt                          >  2:5462668/1‑101 (MQ=255)
                                                                   cAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGt                          >  2:5018792/1‑101 (MQ=255)
                                                                    cacccGCGCACTGCCGCCGCCGTCTCGCGGCGACAGCTCGGCGCCCGGATCCCCGCCTCCAGACCCTTTAAATTCTTTGCCTATTCCTGGGGCGTTCTGTc                         <  2:811106/98‑1 (MQ=255)
                                                                       ccGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGa                      >  1:3510750/1‑101 (MQ=255)
                                                                       ccGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGa                      <  2:2801211/101‑1 (MQ=255)
                                                                        cgcgCAAGGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGaa                     <  2:3291744/101‑1 (MQ=255)
                                                                          cgcAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAAcc                   >  2:1214680/1‑101 (MQ=255)
                                                                           gcAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGACCCg                  <  1:7206014/101‑1 (MQ=255)
                                                                           gcAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCg                  >  1:3923721/1‑101 (MQ=255)
                                                                            cAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGc                 <  1:1040049/101‑1 (MQ=255)
                                                                              aTGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGctc               >  1:2803053/1‑101 (MQ=255)
                                                                                gccgccGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCtctc             <  1:8693576/101‑1 (MQ=255)
                                                                                  cgccgcGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCgg           <  2:8405203/101‑1 (MQ=255)
                                                                                   gccgcGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGa          <  1:9088322/101‑1 (MQ=255)
                                                                                     cgcgGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGaca        <  2:1788424/101‑1 (MQ=255)
                                                                                      gcgGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGacac       <  1:1082853/101‑1 (MQ=255)
                                                                                       cgGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACg      >  2:7175647/1‑101 (MQ=255)
                                                                                       cgGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACg      <  2:7930062/101‑1 (MQ=255)
                                                                                       cgGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACg      >  1:1173678/1‑101 (MQ=255)
                                                                                        gggCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACGc     <  1:8856697/101‑1 (MQ=255)
                                                                                              ||                                                                                                
ACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACGCAAT  >  NC_014034/1802183‑1802374

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG