Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,836,366 2 bp→GC 100% coding (502‑503/1257 nt) pdhC ← pyruvate dehydrogenase complex E2 component dihydrolipoyllysine‑residue acetyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,836,3660A→G100.0% 1121.8 317L168P (CTT→CCT) pdhCpyruvate dehydrogenase complex E2 component dihydrolipoyllysine‑residue acetyltransferase
Reads supporting (aligned to +/- strand):  new base (180/137):  ref base (0/0):  total (180/137)
*NC_0140341,836,3670G→C100.0% 1093.1 317L168V (CTT→GTT) pdhCpyruvate dehydrogenase complex E2 component dihydrolipoyllysine‑residue acetyltransferase
Reads supporting (aligned to +/- strand):  new base (180/137):  ref base (0/0):  total (180/137)

Only 100 of 372 total aligned reads displayed.

GCATGCCATCGAGCGGGATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAAGGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGACCCCGAAATCGCCGCCAGATCC  >  NC_014034/1836271‑1836467
                                                                                               ||                                                                                                    
gCATGCCATCGAGCGGGATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGAcc                                                                                                  <  2:1152203/101‑1 (MQ=255)
 cATGCCATCGAGCGGGATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCg                                                                                                 >  2:2347159/1‑101 (MQ=255)
  aTGCCATCGAGCGGGATCTCGGTATAGGCCCGGGCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGGGCCGGAGCAGGGGCCGCCACAGCGACCgg                                                                                                >  1:6444568/1‑101 (MQ=255)
  aTGCCATCGAGCGGGATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCgg                                                                                                >  1:6316364/1‑101 (MQ=255)
  aTGCCATCGAGCGGGATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCgg                                                                                                <  2:6059663/101‑1 (MQ=255)
   tGCCATCGAGCGGGATCTCGGTATAGGCCCGGCCCTTATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGa                                                                                               >  1:2690663/1‑101 (MQ=255)
    gCCATCGAGCGGGATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAg                                                                                              <  1:7157063/101‑1 (MQ=255)
     ccATCGAGCGGGATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAgc                                                                                             >  2:5202658/1‑101 (MQ=255)
        tCGAGCGGGATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAgccgc                                                                                          <  1:3764853/101‑1 (MQ=255)
          gAGCGGGATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGccgccg                                                                                        <  2:4062613/101‑1 (MQ=255)
           aGCGGGATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCcgccgc                                                                                       <  2:2052368/101‑1 (MQ=255)
             cGGGATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAg                                                                                     >  2:4217922/1‑101 (MQ=255)
              gggATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAgg                                                                                    <  1:5550615/101‑1 (MQ=255)
              gggATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAgg                                                                                    >  2:5471163/1‑101 (MQ=255)
               ggATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGgc                                                                                   >  1:8050729/1‑101 (MQ=255)
               ggACCTCGGTATGGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGgc                                                                                   <  2:5784594/101‑1 (MQ=255)
                   ctcGGTATAGGCGCGGCCCTCATAAAGCGTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAAGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCgg                                                                               >  1:6512039/1‑101 (MQ=255)
                   ctcGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCgg                                                                               >  2:3889642/1‑101 (MQ=255)
                    tcGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCggg                                                                              >  1:6007032/1‑101 (MQ=255)
                     cGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGt                                                                             >  2:1275119/1‑101 (MQ=255)
                      ggaataGGCCCGGCCCTCATAAGGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGtt                                                                            <  2:3226060/98‑1 (MQ=255)
                       gTATAGGCCCGGCCCTCATAAAGCTTCGCGCCCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGttt                                                                           <  2:6468454/101‑1 (MQ=255)
                       gTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGttt                                                                           <  1:3129158/101‑1 (MQ=255)
                       gTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGtct                                                                           <  2:7873685/101‑3 (MQ=255)
                        tataGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTc                                                                          >  1:2203438/1‑101 (MQ=255)
                         atgggcccggcccTAATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCg                                                                         <  1:3969487/98‑1 (MQ=255)
                         atcggcccggcccTCATAAAGCTTCGCGCCCGCCGCAGCGGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCg                                                                         <  2:9123261/98‑1 (MQ=255)
                         ataGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGGGCGGGGTTcc                                                                         >  2:314054/1‑100 (MQ=255)
                          taGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGc                                                                        >  1:1985013/1‑101 (MQ=255)
                          taGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGc                                                                        >  2:7020566/1‑101 (MQ=255)
                          taGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGc                                                                        <  2:4798402/101‑1 (MQ=255)
                          atggcccgccccCCATAAAGCTTCGCGCCCGCCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGc                                                                        <  2:1047916/99‑1 (MQ=255)
                            ggcccggcccTCATAAAGCTTCGCGGCCGTCGCAGCCGAGGCGGGTGCCGGAGCGGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCa                                                                      <  1:5919903/101‑1 (MQ=255)
                            ggcccggcccTCATAAAGCTTCGCGCCCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCa                                                                      <  2:2154178/101‑1 (MQ=255)
                              cccggcccTCATAAAGCTTCGCGACCGTCGCACCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCAcc                                                                    >  1:6049005/1‑101 (MQ=255)
                              cccggcccCCAAAAAGCTTCGCGCCCGTCGCAGCCGAGCGGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCAcc                                                                    <  1:8796754/101‑1 (MQ=255)
                               ccggcccTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACcg                                                                   >  1:9169565/1‑101 (MQ=255)
                                    ccTCATAAGGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCt                                                              <  2:5950823/101‑1 (MQ=255)
                                      tCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTc                                                            >  2:7560821/1‑101 (MQ=255)
                                      tCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTc                                                            >  1:1825681/1‑101 (MQ=255)
                                      tCATAAAGCTCCGCGACCGTCGCAGCCGAGACCGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTc                                                            <  1:4382707/101‑1 (MQ=255)
                                      ccATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCGGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTc                                                            <  2:3580488/100‑1 (MQ=255)
                                       caaaaaGCTGCGCGCCGCACGAAGACGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGCCCGGAGCCGCCGCAGGCGCGGGTTTCGCAACCGCGCCTTCg                                                           <  1:6540719/98‑1 (MQ=255)
                                       cATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGGGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCg                                                           >  1:2068599/1‑101 (MQ=255)
                                       cATAAAGCTTCGCGACCGTCGCAGCAGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCg                                                           <  2:63163/101‑1 (MQ=255)
                                       cATAAAGCTCCGCGCCCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCg                                                           <  1:1217469/101‑1 (MQ=255)
                                        aTAAAGCTTCGCGACCGTCGCAGCCGAGATGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGa                                                          <  2:5021973/101‑1 (MQ=255)
                                          aaaGCTTCGCGCCCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACa                                                        <  1:4758229/101‑1 (MQ=255)
                                          aaaCCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACa                                                        <  2:781677/101‑1 (MQ=255)
                                             gCTCCGCGCCCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCg                                                     <  1:1757652/101‑1 (MQ=255)
                                              ctccgcgCCCGTCGCAGCCGAGACGGGTGCCGGAGCGGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCgg                                                    <  1:5469747/98‑1 (MQ=255)
                                                 cgcgACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCt                                                 <  1:5163510/101‑1 (MQ=255)
                                                 cgcgACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCt                                                 >  1:6151120/1‑101 (MQ=255)
                                                  gcgCCCTTCGCGGCGGAGCCGGGTGCGGGCGCGGGGGCCGCCACAGCGCCCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCtt                                                <  2:8702614/101‑1 (MQ=255)
                                                  gccccccgcGCGGCCGAGACGGGTGCCGGAGCAGGGGCCGCCCCAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCtt                                                <  1:325128/93‑1 (MQ=255)
                                                  gcccccGCCGCCGCCGAGCCGGGTGCCGGAGCGGGGGCCCCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCtt                                                <  1:428158/97‑1 (MQ=255)
                                                  ccccccGTCCCAGCAGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGCCCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCtt                                                <  1:1114913/97‑1 (MQ=255)
                                                   cgcccGTCGCAGCCGAGGCGGGTGCGGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTg                                               <  1:4500810/98‑1 (MQ=255)
                                                   cgcccGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTg                                               <  1:6999996/98‑1 (MQ=255)
                                                   cgcccGGCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTg                                               <  2:2993074/98‑1 (MQ=255)
                                                   cgcccGCCGCAGCCGAGACGGGTGCCGGAGCGGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTg                                               <  1:2541084/98‑1 (MQ=255)
                                                   cgACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTg                                               <  2:4557995/101‑1 (MQ=255)
                                                    gcccGCCGCACCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGa                                              <  2:8009587/99‑1 (MQ=255)
                                                     aCCGTGCCAGCGGAGACGGGGCCCGGGGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGAc                                             <  2:4153831/101‑1 (MQ=255)
                                                      tgtgcGCAGCAGCGGCGGGGTCCGGACCGGGGGCCGCCCCAGGGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACg                                            <  1:2804044/97‑1 (MQ=255)
                                                      ccGTCCCAGCCGAGACGGGTGCGGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACg                                            <  2:722223/101‑1 (MQ=255)
                                                      ccGCCGCGGCCGAGACGGGTGCCGGAGCAGGGCCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACg                                            <  2:6746019/101‑1 (MQ=255)
                                                        gTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGac                                          >  2:1750719/1‑100 (MQ=255)
                                                          cGCGGCAGAGAGGGGTGGGGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATcc                                        <  2:4397786/101‑1 (MQ=255)
                                                          cGCAGCCGAGACGGGTGCCGGAGCGGGGCCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATcc                                        <  1:5360598/101‑1 (MQ=255)
                                                             ggCCGAGGCGGGTGCCGGAGCGGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGcc                                     <  1:275560/100‑1 (MQ=255)
                                                             aGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGcc                                     >  2:818466/1‑101 (MQ=255)
                                                                cgggccGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCAt                                  <  1:7859448/96‑1 (MQ=255)
                                                                cGAGACGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCAt                                  >  2:4748440/1‑101 (MQ=255)
                                                                 gagaCGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATg                                 >  2:2511342/1‑101 (MQ=255)
                                                                 gagaCGGGTGCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATg                                 >  2:7089636/1‑101 (MQ=255)
                                                                  ggccGGGGGCGGGAGCGGGGGCCGCCACAGCGCCCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGc                                <  2:2644015/98‑1 (MQ=255)
                                                                    aCGGGTGCGGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCgg                              <  1:5050739/101‑1 (MQ=255)
                                                                      gggTGCGGGGGGGGGGCCCGCCACAGCGCCCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGc                            <  1:8317607/101‑1 (MQ=255)
                                                                      gggTGCCGGAGCGGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGc                            <  1:8465710/101‑1 (MQ=255)
                                                                         tGCCGGAGCAGGGGCCGCCACAGCGACCGGAGTCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCgg                         >  1:7759918/1‑101 (MQ=255)
                                                                          gCCGGAGCGGGGGCCGCCACAGCGACCGGAGCTGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGa                        <  1:4549620/101‑1 (MQ=255)
                                                                          gCCGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGa                        >  2:1643117/1‑101 (MQ=255)
                                                                           cgggAGCGGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGAc                       <  1:4143389/99‑1 (MQ=255)
                                                                           ccGGAGCGGGGCCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGAc                       <  1:7317804/101‑1 (MQ=255)
                                                                           ccGGAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGAc                       >  1:7069928/1‑101 (MQ=255)
                                                                             ggggCAGGGCCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGAccc                     <  2:1406859/98‑1 (MQ=255)
                                                                             ggAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGAccc                     >  1:8614320/1‑101 (MQ=255)
                                                                             ggAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGAccc                     >  2:8865349/1‑101 (MQ=255)
                                                                             ggAGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGGTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCCTGCTGGCCTGAccc                     >  2:8439579/1‑101 (MQ=255)
                                                                               aGCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCCAACCCCg                   >  1:1651664/1‑101 (MQ=255)
                                                                                gCAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGACCCCGa                  >  2:3329253/1‑101 (MQ=255)
                                                                                 cAGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGACCCCGaa                 >  1:8910476/1‑101 (MQ=255)
                                                                                  aGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGACCCCGaaa                >  1:7635854/1‑101 (MQ=255)
                                                                                  aGGGGCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGACCCCGaaa                >  1:3541365/1‑101 (MQ=255)
                                                                                   ggggCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGACCCCGaaac               >  1:3327391/1‑100 (MQ=255)
                                                                                     ggCCGCCACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGACCCCGAAATcg             >  1:2326770/1‑101 (MQ=255)
                                                                                     agccgccACAGCGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGAGGGCCGGACCCCGAAATcg             >  2:2873880/2‑101 (MQ=255)
                                                                                        cgccACAGCGCCCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGACCCCGAAATcgccg          <  1:3716462/101‑1 (MQ=255)
                                                                                                ggACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGACCCCGAAATCGCCGCCAGATcc  <  2:306307/101‑1 (MQ=255)
                                                                                               ||                                                                                                    
GCATGCCATCGAGCGGGATCTCGGTATAGGCCCGGCCCTCATAAAGCTTCGCGACCGTCGCAGCCGAGACGGGTGCCGGAGCAGGGGCCGCCACAAGGACCGGAGCCGCCGCAGGCGCGGGTTTCGCCACCGCGCCTTCGACATCGGCCTTGACGATCCGCCCATGCGGGCCGGACCCCGAAATCGCCGCCAGATCC  >  NC_014034/1836271‑1836467

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG