Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,053,078 2 bp→GC 100% coding (4156‑4157/6198 nt) RCAP_rcc01900 ← hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,053,0780C→G100.0% 1345.2 366R1386P (CGG→CCG) RCAP_rcc01900hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (186/180):  ref base (0/0):  total (186/180)
*NC_0140342,053,0790G→C100.0% 1347.5 366R1386G (CGG→GGG) RCAP_rcc01900hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (186/180):  ref base (0/0):  total (186/180)

Only 100 of 388 total aligned reads displayed.

TCCAGGATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCCGTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCACG  >  NC_014034/2052979‑2053173
                                                                                                   ||                                                                                              
    ggttGTCGTTGCCCGCGCCGCCAAGGATGCGGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCg                                                                                            <  2:3739983/98‑1 (MQ=37)
    ggATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCg                                                                                            <  1:7649194/101‑1 (MQ=255)
     gATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGc                                                                                           >  2:3089874/1‑101 (MQ=255)
      aTGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGcc                                                                                          >  1:4855026/1‑101 (MQ=255)
       tGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCg                                                                                         <  1:4007718/101‑1 (MQ=255)
       tGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCg                                                                                         >  1:9014391/1‑101 (MQ=255)
        gTCGTGGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGa                                                                                        <  2:6061520/101‑1 (MQ=255)
         tCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAg                                                                                       <  2:1652775/101‑1 (MQ=255)
           gTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGa                                                                                     >  1:2169885/1‑101 (MQ=255)
            ttGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGCGTCATCGCCCGCTTCGCCGAGGAt                                                                                    >  1:7099557/1‑101 (MQ=255)
             tGCCCGCGCCGCCAAGGATGCTGTCGTCATCCGGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATg                                                                                   <  1:5982453/101‑1 (MQ=255)
             tGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATg                                                                                   <  2:6315083/101‑1 (MQ=255)
             tGCCCGCCCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATg                                                                                   <  2:7003224/101‑1 (MQ=255)
              gCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATgg                                                                                  <  2:8513817/101‑1 (MQ=255)
               cccGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCCGCGTGTCATGGCCGCCTTCGCCGAGGATggg                                                                                 >  2:4286868/1‑100 (MQ=38)
                ccGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGgtg                                                                                >  2:8828034/1‑101 (MQ=255)
                 cgcgCCGCCAAGGATACTGTCGTCATCCAGCCCGCCGGGGCTGCTGTCATTGCCCGCGCCACCATCCCGCGTGTCATCGCCCGCTTCGCCGAGGATGgtgc                                                                               >  2:4640245/1‑100 (MQ=37)
                   cgccgcCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCg                                                                             <  1:3486572/101‑1 (MQ=255)
                    cccgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGt                                                                            <  2:7549160/100‑1 (MQ=255)
                     ccgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGtt                                                                           <  1:7280916/101‑1 (MQ=255)
                     ccgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGtt                                                                           <  2:898442/101‑1 (MQ=255)
                     ccgccAAGGATGCTGCCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGtt                                                                           <  2:3622876/101‑1 (MQ=255)
                      cgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTg                                                                          <  2:826536/101‑1 (MQ=255)
                       gccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGTTGGTGTCGTTGc                                                                         <  1:8070457/101‑1 (MQ=255)
                        ccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGcc                                                                        >  1:8536192/1‑101 (MQ=255)
                        ccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGcc                                                                        >  1:1931930/1‑101 (MQ=255)
                         cAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCGTCGCCCGCTTCGCCGAGGATGGTGTCGTTGccc                                                                       <  1:6471153/101‑1 (MQ=255)
                         cAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGccc                                                                       >  2:720049/1‑101 (MQ=255)
                          aaGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCt                                                                      <  1:361427/101‑1 (MQ=255)
                           aGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTCTCGTTGCCCTc                                                                     <  1:5740384/101‑1 (MQ=255)
                           aGGATGCTGTCGTCATCCAGCCCGCCGGCGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTc                                                                     >  2:4788157/1‑101 (MQ=255)
                            ggctGCTCTCTTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTTTCGTTGCCCTcc                                                                    <  2:5087909/98‑1 (MQ=37)
                            ggATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcc                                                                    <  1:429700/101‑1 (MQ=255)
                            ggATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcc                                                                    >  1:2784524/1‑101 (MQ=255)
                            ggATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcc                                                                    >  2:4049628/1‑101 (MQ=255)
                             gATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCCCCACCGTCCAGCGCGTCATCGCCCGCTTCGCCGAGGGTGGTGTCGTTGCCCTcgc                                                                   >  2:5360742/1‑99 (MQ=37)
                             gATGCTGTCGTCACCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTccc                                                                   <  2:7311136/101‑1 (MQ=255)
                              aTGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcccc                                                                  >  1:6164896/1‑101 (MQ=255)
                               tGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCg                                                                 <  2:3412207/101‑1 (MQ=255)
                                  tGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGccc                                                              <  1:2010620/101‑1 (MQ=255)
                                  tGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGccc                                                              >  2:2439769/1‑101 (MQ=255)
                                  tGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGccc                                                              <  1:4700901/101‑1 (MQ=255)
                                  cgttcttaTCCACCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGccc                                                              <  2:1244736/94‑1 (MQ=37)
                                   gtcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCa                                                             <  2:5461243/101‑1 (MQ=255)
                                   gtcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCa                                                             >  2:5867006/1‑101 (MQ=255)
                                   gtcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCa                                                             >  2:7801394/1‑101 (MQ=255)
                                    tcgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAg                                                            >  1:9181791/1‑101 (MQ=255)
                                     cgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGc                                                           >  2:6645170/1‑101 (MQ=255)
                                     cgtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGc                                                           <  1:3020097/101‑1 (MQ=255)
                                      gtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCa                                                          >  1:5562189/1‑101 (MQ=255)
                                      gtcACCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCa                                                          <  1:241820/101‑1 (MQ=255)
                                       tcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCAc                                                         >  1:5434446/1‑101 (MQ=255)
                                        cATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCAcc                                                        <  2:4459289/101‑1 (MQ=255)
                                         ccccAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCg                                                       <  2:6831578/99‑1 (MQ=255)
                                           ccAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAg                                                     <  2:2136350/101‑1 (MQ=255)
                                             aGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTc                                                   >  1:8006895/1‑101 (MQ=255)
                                                ccgccgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCTAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATc                                                <  2:1115960/101‑1 (MQ=255)
                                                    cgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGccc                                            <  1:3155926/101‑1 (MQ=255)
                                                    cgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGccc                                            >  2:2585711/1‑101 (MQ=255)
                                                        gATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGc                                        >  1:229441/1‑101 (MQ=255)
                                                        gATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGc                                        >  1:258919/1‑101 (MQ=255)
                                                         aTGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGcc                                       >  1:1490182/1‑101 (MQ=255)
                                                         aTGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGcc                                       <  1:3254628/101‑1 (MQ=255)
                                                          tGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGccc                                      <  1:8777182/101‑1 (MQ=255)
                                                           gCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATAGCCCGAGCCCg                                     >  2:531484/1‑101 (MQ=255)
                                                             tGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGcc                                   <  1:137028/101‑1 (MQ=255)
                                                             tGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGcc                                   >  1:1616408/1‑101 (MQ=255)
                                                             tGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGcc                                   >  2:7151357/1‑101 (MQ=255)
                                                             tGTCATTGCCCGCGCCACCGGCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGcc                                   <  2:5504160/101‑1 (MQ=255)
                                                               tCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtc                                 <  2:4854866/101‑1 (MQ=255)
                                                                cATTGCCCGCGCCCCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcg                                <  1:1092936/101‑1 (MQ=255)
                                                                cATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcg                                >  1:6135119/1‑101 (MQ=255)
                                                                 ggtcagcgcgCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcga                               >  2:1429871/7‑101 (MQ=38)
                                                                 cttccccGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcga                               <  1:6772413/97‑1 (MQ=255)
                                                                 aTTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgc                               >  2:8723088/1‑100 (MQ=255)
                                                                 aTTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcga                               >  1:6680514/1‑101 (MQ=255)
                                                                    gCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcg                            <  1:5363024/101‑1 (MQ=255)
                                                                    gCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcg                            <  1:1208845/101‑1 (MQ=255)
                                                                    gCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcg                            >  2:8116560/1‑101 (MQ=255)
                                                                    gCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGGGTCGTTGCCCCCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcg                            <  1:5474958/101‑1 (MQ=38)
                                                                     cccGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcga                           <  2:6670622/101‑1 (MQ=255)
                                                                     cccGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcga                           <  1:6900550/101‑1 (MQ=255)
                                                                      ccGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGaa                          <  1:7850889/101‑1 (MQ=255)
                                                                       cgcgCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAt                         <  2:7954665/101‑1 (MQ=255)
                                                                       cgcgCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAt                         <  1:105409/101‑1 (MQ=255)
                                                                        gcgcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtc                        <  2:9181369/101‑1 (MQ=255)
                                                                            cACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgtcg                    >  1:83487/1‑101 (MQ=255)
                                                                                gTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCgg                >  2:2754004/1‑101 (MQ=255)
                                                                                gTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCgg                >  2:2304175/1‑101 (MQ=255)
                                                                                 tCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGt               >  2:3259102/1‑101 (MQ=255)
                                                                                 tCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGt               <  1:1889248/101‑1 (MQ=255)
                                                                                  ccAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGACTCGTCGCCGGTg              >  2:1893122/1‑101 (MQ=255)
                                                                                     gCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGccc           >  1:5240643/1‑101 (MQ=255)
                                                                                       gtgtCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCcgc         >  2:6507302/1‑101 (MQ=255)
                                                                                         gtCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGt       >  1:4975075/1‑101 (MQ=255)
                                                                                          tCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGtt      <  2:1490135/101‑1 (MQ=255)
                                                                                           cgtCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTc     <  1:7431117/99‑1 (MQ=255)
                                                                                            aTCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCa    >  1:7591519/1‑101 (MQ=255)
                                                                                             ccGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCCCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCAc   <  1:3831042/100‑1 (MQ=255)
                                                                                              cGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCCCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCACg  <  1:4516282/101‑1 (MQ=255)
                                                                                                   ||                                                                                              
TCCAGGATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCCGTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCACG  >  NC_014034/2052979‑2053173

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG