Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,157,926 2 bp→AC 100% intergenic (‑298/‑483) RCAP_rcc02004 ← / → RCAP_rcc02005 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,157,9260C→A100.0% 149.6 491intergenic (‑298/‑484)RCAP_rcc02004/RCAP_rcc02005hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (208/232):  ref base (45/6):  total (253/238)
Fisher's exact test strand distribution p-value = 9.82e-09
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00
*NC_0140342,157,9270G→C100.0% 126.6 491intergenic (‑299/‑483)RCAP_rcc02004/RCAP_rcc02005hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (208/232):  ref base (45/6):  total (253/238)
Fisher's exact test strand distribution p-value = 9.82e-09
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 503 total aligned reads displayed.

GAGGCTCTCCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.ACGGCCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCCGTCGTCTT  >  NC_014034/2157828‑2158023
                                                                                                   ||                                                                                                
gAGGCTCTCCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGAGGCACAGGAGAGGCCGAGTAAAATC.ACgg                                                                                                 >  2:4209315/1‑101 (MQ=37)
      ctcCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCg                                                                                           >  2:5836129/1‑101 (MQ=255)
         ccAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGCGACCGATGAAAATC.AACGGCTTCGGtt                                                                                        >  1:8981125/1‑101 (MQ=38)
          cAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTc                                                                                       <  2:7693723/101‑1 (MQ=255)
          cAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTc                                                                                       <  1:8846187/101‑1 (MQ=255)
           aGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCa                                                                                      >  2:4764211/1‑101 (MQ=255)
             cATACCAAACCGATGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGt                                                                                    >  1:6505249/1‑101 (MQ=38)
              aTACCAAACCGCTGGCGACCATCAGGTTCAGTCCCCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTc                                                                                   >  1:4380678/1‑101 (MQ=38)
               tACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGAAGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTcc                                                                                  >  1:7058580/1‑101 (MQ=38)
                aCCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTccg                                                                                 >  1:7940353/1‑101 (MQ=255)
                  cAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTccgcc                                                                               <  1:3512977/101‑1 (MQ=255)
                        gCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCcgtcggc                                                                         >  1:2734945/1‑99 (MQ=255)
                          tGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCtt                                                                       <  1:8755952/101‑1 (MQ=255)
                          tGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCtt                                                                       >  2:7525583/1‑101 (MQ=255)
                           ggCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTg                                                                      >  2:5040082/1‑101 (MQ=255)
                           ggCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTg                                                                      <  1:7347628/101‑1 (MQ=255)
                           ggCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTg                                                                      >  1:1491031/1‑101 (MQ=255)
                           ggCGACCATCAGGTTCAGTCCGCCGTCGTCTTCGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTg                                                                      <  2:6764646/101‑1 (MQ=38)
                            gCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTgg                                                                     <  2:2081087/101‑1 (MQ=255)
                             cGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGc                                                                    >  1:8351320/1‑101 (MQ=255)
                              gACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGAGGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCg                                                                   >  2:4451481/1‑101 (MQ=37)
                               aCCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCgg                                                                  >  1:1012041/1‑101 (MQ=255)
                               aCCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCgg                                                                  >  2:3597734/1‑101 (MQ=255)
                                ccATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGt                                                                 <  2:2196519/101‑1 (MQ=255)
                                 cATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTg                                                                >  1:3316527/1‑101 (MQ=255)
                                   tCAGGTTCAGTCCCCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGAc                                                              <  2:1331934/101‑1 (MQ=38)
                                     agcgtcggTCCGACGTGGTCCTGGAGGTTATGTGATGGACAGGAAAGCCAGACGAAGATA.ATAGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGt                                                            <  2:2778363/94‑1 (MQ=12)
                                     aGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACgg                                                            >  1:3200198/1‑100 (MQ=255)
                                      ggTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTg                                                           <  1:7572823/101‑1 (MQ=255)
                                          cAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGc                                                       >  1:8496623/1‑101 (MQ=255)
                                           aGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCa                                                      >  1:6189380/1‑101 (MQ=255)
                                           aGTCCGCCGTCGTCTGGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCa                                                      <  2:4994197/101‑1 (MQ=38)
                                             tCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCAcc                                                    >  1:6771378/1‑101 (MQ=255)
                                              ccgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCt                                                   >  2:4896985/1‑101 (MQ=255)
                                               cgccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTa                                                  >  2:4338589/1‑101 (MQ=255)
                                                gccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTaa                                                 <  1:197879/101‑1 (MQ=255)
                                                 ccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTaaa                                                <  1:4533780/101‑1 (MQ=255)
                                                 ccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTaaa                                                <  2:1704647/101‑1 (MQ=255)
                                                 ccgTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTaaa                                                <  2:3239778/101‑1 (MQ=255)
                                                   gtcgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACa                                              <  1:2631695/101‑1 (MQ=255)
                                                     cgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAag                                            >  1:931759/1‑101 (MQ=255)
                                                     cgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAag                                            >  2:5434292/1‑101 (MQ=255)
                                                     cgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAag                                            <  1:8650020/101‑1 (MQ=255)
                                                     cgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAag                                            >  2:8168785/1‑101 (MQ=255)
                                                     cgtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAag                                            >  1:170226/1‑101 (MQ=255)
                                                      gtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAaga                                           <  1:908744/101‑1 (MQ=255)
                                                      gtcTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAaga                                           >  1:234581/1‑101 (MQ=255)
                                                       tcTTGGCTGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAagag                                          <  2:4225616/101‑1 (MQ=38)
                                                        cTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAgg                                         >  1:6331983/1‑101 (MQ=255)
                                                        cTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAgg                                         >  1:5560471/1‑101 (MQ=255)
                                                         ttGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGt                                        <  2:718140/101‑1 (MQ=255)
                                                         ttGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTAGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGt                                        >  1:8117689/1‑101 (MQ=38)
                                                           ggCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTc                                      >  1:7736205/1‑101 (MQ=255)
                                                           ggCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTc                                      >  2:3712780/1‑101 (MQ=255)
                                                           ggCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTc                                      <  1:129072/101‑1 (MQ=255)
                                                           ggCGGTGATGTGAGGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTc                                      <  2:2447277/101‑1 (MQ=38)
                                                            gCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCt                                     <  1:53369/101‑1 (MQ=255)
                                                             cGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTa                                    <  1:5836364/101‑1 (MQ=255)
                                                             cGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTa                                    <  1:1866971/101‑1 (MQ=255)
                                                               gtgatgtgatGCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACg                                  >  2:7802373/1‑101 (MQ=255)
                                                               gtgatgtgatGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGGGGTGACGTGAGGCACCTAAACAAAAGGTTCTCCg                                  >  2:2907308/1‑101 (MQ=37)
                                                               gtgatgtgatGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACg                                  >  1:4281716/1‑101 (MQ=255)
                                                               gtgatgtgatGCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACg                                  <  2:8876189/101‑1 (MQ=255)
                                                                 gatgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGAt                                >  2:8490964/1‑101 (MQ=255)
                                                                 gatgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGAt                                >  1:5777905/1‑101 (MQ=255)
                                                                  atgtgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATg                               >  2:6372149/1‑101 (MQ=255)
                                                                  atgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATg                               <  1:2497449/101‑1 (MQ=255)
                                                                   tgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGc                              <  2:3333427/101‑1 (MQ=255)
                                                                   tgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGc                              <  2:8350957/101‑1 (MQ=255)
                                                                   tgtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGc                              <  1:4176651/101‑1 (MQ=255)
                                                                   tgtgatgCACAGGAGAGACCGAGGAAAATG.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGc                              <  2:5127759/101‑1 (MQ=37)
                                                                    gtgatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGcc                             <  1:3154865/101‑1 (MQ=255)
                                                                     tgatgCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCg                            >  2:4012834/1‑101 (MQ=255)
                                                                      gatgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACACGAGGTTCAAGGATGCCGa                           >  2:8951836/1‑101 (MQ=37)
                                                                       atgCACAGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGaa                          >  1:6962096/1‑101 (MQ=255)
                                                                         gCACAGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAg                        >  1:4792089/1‑101 (MQ=255)
                                                                           acaGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAggg                      >  2:231370/1‑101 (MQ=255)
                                                                           acaGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAggg                      >  2:1607185/1‑101 (MQ=255)
                                                                           acaGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAggg                      >  2:2577663/1‑101 (MQ=255)
                                                                            caGGAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAgggg                     <  1:3986433/101‑1 (MQ=255)
                                                                             aGGAGAGACCGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGt                    >  2:6559733/1‑101 (MQ=255)
                                                                              ggAGAGACGGATGAAAATC.AACGGCTGCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCCCCTAAACAAGAGGTTCTACGATGCCGAAAGGGGtt                   <  1:3742777/101‑1 (MQ=37)
                                                                              ggAGAGACCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGtt                   <  2:1282244/101‑1 (MQ=255)
                                                                               gagagaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTc                  <  2:4572565/101‑1 (MQ=255)
                                                                               gagagaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTc                  <  2:7274614/101‑1 (MQ=255)
                                                                                 gagaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAg                >  1:7486123/1‑101 (MQ=255)
                                                                                 gagaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAg                <  2:8064769/101‑1 (MQ=255)
                                                                                  agaCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGt               <  2:5777670/101‑1 (MQ=255)
                                                                                    aCCGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTcc             <  1:2287996/101‑1 (MQ=255)
                                                                                     ccGATGAAAATCAACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccg            >  1:5970426/1‑101 (MQ=255)
                                                                                      cGATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccgc           >  2:5622314/1‑101 (MQ=255)
                                                                                       gATGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccgcc          >  2:5354771/1‑101 (MQ=255)
                                                                                        aTGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTccgccg         >  2:8645426/1‑101 (MQ=255)
                                                                                         tGAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgt        >  1:5207521/1‑101 (MQ=255)
                                                                                          gAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtc       <  1:4999427/101‑1 (MQ=255)
                                                                                          gAAAATC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtc       <  1:287027/101‑1 (MQ=255)
                                                                                           aaaaTC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtcg      <  2:6126244/101‑1 (MQ=255)
                                                                                           aaaaTC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtcg      >  1:4963671/1‑101 (MQ=255)
                                                                                            aaaTC.AACGGCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCcgtcgt     >  2:1151767/1‑101 (MQ=255)
                                                                                              aTC.ACGG.CTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCCGTCGTCtt  >  2:7031323/1‑101 (MQ=255)
                                                                                                   ||                                                                                                
GAGGCTCTCCCAGCATACCAAACCGCTGGCGACCATCAGGTTCAGTCCGCCGTCGTCTTGGCGGTGATGTGATGCACAGGAGAGACCGATGAAAATC.ACGGCCTTCGGTTCAGTCCGCCGTCGTCTTGGCGGTGACGTGAGGCACCTAAACAAGAGGTTCTACGATGCCGAAAGGGGTTCAGTCCGCCGTCGTCTT  >  NC_014034/2157828‑2158023

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG