Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,289 2 bp→AT 100% coding (87‑88/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2890T→A100.0% 1395.7 364T30S (ACA→TCA) rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (209/155):  ref base (0/0):  total (209/155)
*NC_0140342,177,2900A→T100.0% 1392.3 360C29* (TGT→TGArbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (205/154):  ref base (0/0):  total (205/155)

Only 100 of 369 total aligned reads displayed.

CATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCA  >  NC_014034/2177202‑2177378
                                                                                             ||                                                                                             
 aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAgg                                                                                       >  1:4588309/1‑101 (MQ=255)
 aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAgg                                                                                       >  1:8122877/1‑101 (MQ=255)
   aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGcc                                                                                     >  2:8346255/1‑101 (MQ=255)
   aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGcc                                                                                     >  2:726787/1‑101 (MQ=255)
   aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGcc                                                                                     <  2:5274550/101‑1 (MQ=255)
   aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGcc                                                                                     <  2:320861/101‑1 (MQ=255)
    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc                                                                                    >  1:2466862/1‑101 (MQ=255)
    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc                                                                                    <  2:4961274/101‑1 (MQ=255)
    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc                                                                                    >  2:4162952/1‑101 (MQ=255)
    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGccc                                                                                    >  1:3105730/1‑101 (MQ=255)
     gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGcccc                                                                                   <  1:836497/101‑1 (MQ=255)
       cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCgg                                                                                 >  2:8005793/1‑101 (MQ=255)
       cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCgg                                                                                 >  2:3019607/1‑101 (MQ=255)
         agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGtt                                                                               >  2:6353964/4‑101 (MQ=255)
          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGttt                                                                              <  2:589562/99‑1 (MQ=255)
          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGttt                                                                              >  1:4536529/3‑101 (MQ=255)
            gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCg                                                                            <  2:1785599/101‑1 (MQ=255)
             cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCgg                                                                           >  2:6788361/1‑101 (MQ=255)
              aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGc                                                                          >  1:5813738/1‑101 (MQ=255)
               ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGcc                                                                         >  2:2491590/1‑101 (MQ=255)
                    cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.G.aagc                                                                  >  2:2356032/1‑98 (MQ=255)
                       aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGATGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAgccgc                                                                >  2:8206430/1‑101 (MQ=255)
                             gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGc                                                          <  1:8845624/101‑1 (MQ=255)
                             gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGc                                                          >  2:2907909/1‑101 (MQ=255)
                             gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGc                                                          <  1:5910209/101‑1 (MQ=255)
                              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGcc                                                         <  2:9131493/101‑1 (MQ=255)
                              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGcc                                                         <  1:1021478/101‑1 (MQ=255)
                               cGCCTTTGCCGAAGCCCCTTCCTTCATCTGGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCa                                                        <  1:1072412/101‑1 (MQ=255)
                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCa                                                        <  2:5714299/101‑1 (MQ=255)
                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCa                                                        >  2:5348223/1‑101 (MQ=255)
                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGCCCa                                                        >  1:2858727/1‑101 (MQ=255)
                                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAg                                                       <  1:2687224/101‑1 (MQ=255)
                                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAg                                                       >  1:5085175/1‑101 (MQ=255)
                                  cTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCTgcg                                                     <  1:7198496/101‑1 (MQ=255)
                                  cTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAgcg                                                     >  1:4210195/1‑101 (MQ=255)
                                   tttGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAgcgc                                                    <  2:3105631/101‑1 (MQ=255)
                                   tttGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAgcgc                                                    >  1:437215/1‑101 (MQ=255)
                                     tGCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCgg                                                  <  1:2414346/101‑1 (MQ=255)
                                      gCCGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGa                                                 >  1:1675020/1‑101 (MQ=255)
                                       ccGAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGaa                                                <  1:2059697/101‑1 (MQ=255)
                                         gAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGaaac                                              >  2:448216/1‑100 (MQ=255)
                                         gAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGaaaa                                              >  1:6738627/1‑101 (MQ=255)
                                         gAAGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGaaaa                                              >  1:5147831/1‑101 (MQ=255)
                                          aaGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAAc                                             >  1:4955115/1‑101 (MQ=255)
                                          aaGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAAc                                             >  2:6484777/1‑101 (MQ=255)
                                           aGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACg                                            <  1:3019706/101‑1 (MQ=255)
                                           aGCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACg                                            >  2:768948/1‑101 (MQ=255)
                                            gCACCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGc                                           >  1:649730/1‑101 (MQ=255)
                                             cACCTTCCTTCATCTGGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGcc                                          <  2:2086098/101‑1 (MQ=255)
                                              cccttccttCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                         <  2:7104649/100‑1 (MQ=255)
                                              aCCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                         >  2:6994524/1‑101 (MQ=255)
                                              aCCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                         <  2:4020747/101‑1 (MQ=255)
                                              aCCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                         <  1:3362050/101‑1 (MQ=255)
                                              aCCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                         <  1:3970177/101‑1 (MQ=255)
                                              aCCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                         >  1:8527364/1‑101 (MQ=255)
                                              aCCTTCCTTCATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCAGCCCGGGCCGCCGCGGAAAACGcag                                         >  2:1349908/1‑99 (MQ=255)
                                                cttccttcATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                      >  2:6615660/1‑101 (MQ=255)
                                                cttccttcATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                      >  2:5431504/1‑101 (MQ=255)
                                                 ttccttcATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                     >  2:4677321/1‑100 (MQ=255)
                                                  tccttcATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                    >  1:6106931/1‑99 (MQ=255)
                                                  tccttcATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                    >  2:6155784/1‑99 (MQ=255)
                                                  tccttcATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                    >  1:1755987/1‑99 (MQ=255)
                                                    cttcATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                  >  1:6270003/1‑97 (MQ=255)
                                                     ttcATCTTGACGAA.AAGGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                 >  2:3623132/1‑96 (MQ=255)
                                                     ttcATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                 >  1:1461332/1‑96 (MQ=255)
                                                      tcATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                 >  1:5344642/1‑101 (MQ=255)
                                                      tcATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                 >  1:7086308/1‑101 (MQ=255)
                                                      tcATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                 <  1:7704723/101‑1 (MQ=255)
                                                       cATCTTGACGAACAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGG.AGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                >  1:6562830/1‑101 (MQ=255)
                                                       cATCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                >  2:6712324/1‑101 (MQ=255)
                                                        aTCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                              >  2:458375/1‑101 (MQ=255)
                                                         tCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                             <  1:7450325/101‑1 (MQ=255)
                                                         tCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                             <  2:8775108/101‑1 (MQ=255)
                                                         tCTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                             >  2:6317095/1‑101 (MQ=255)
                                                          cTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                            >  2:746267/1‑101 (MQ=255)
                                                          cTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                            >  2:3314162/1‑101 (MQ=255)
                                                          cTTGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                            >  2:1538852/1‑101 (MQ=255)
                                                           ttGACGAA.AAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cccc                           >  2:7745062/1‑101 (MQ=255)
                                                                    acgggttggg..gtcggtcTTGGTGAGCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                  <  2:4259834/92‑1 (MQ=255)
                                                                        gggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAg                >  1:3202591/9‑101 (MQ=255)
                                                                          gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGCAAACGCCAGGGCAGAGGCCCCCAACCGGGCCCG.tt             >  1:2529788/7‑101 (MQ=255)
                                                                          gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt             >  1:2776335/7‑101 (MQ=255)
                                                                          gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt             >  1:7809871/7‑101 (MQ=255)
                                                                          gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt             <  1:9140096/95‑1 (MQ=255)
                                                                           ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt            >  2:3769166/6‑100 (MQ=255)
                                                                            tggtgtcggtcTTGGTGATCTGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg           <  1:5625151/97‑3 (MQ=255)
                                                                            tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg           >  2:3880808/5‑99 (MQ=255)
                                                                            tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg           >  1:950038/5‑99 (MQ=255)
                                                                            tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg           <  2:8532371/97‑3 (MQ=255)
                                                                            tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg           <  1:6156021/97‑3 (MQ=255)
                                                                             ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc          <  2:5680110/98‑4 (MQ=255)
                                                                             ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc          >  2:5575066/4‑98 (MQ=255)
                                                                             ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc          >  2:1237372/4‑98 (MQ=255)
                                                                             ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc          <  1:8972545/98‑4 (MQ=255)
                                                                                gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca        >  2:2737188/1‑101 (MQ=255)
                                                                                 tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcat       >  1:3518739/1‑101 (MQ=255)
                                                                                  cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc      >  2:5159698/1‑101 (MQ=255)
                                                                                  cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc      <  2:7395850/101‑1 (MQ=255)
                                                                                  cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc      >  2:3395883/1‑101 (MQ=255)
                                                                                  cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc      >  2:9015895/1‑101 (MQ=255)
                                                                                             ||                                                                                             
CATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAA.AA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCA  >  NC_014034/2177202‑2177378

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG