Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,185 2 bp→GC 100% coding (874‑875/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,1850C→G100.0% 1399.9 377R292G (CGC→GGC) RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (192/184):  ref base (0/0):  total (192/185)
*NC_0140342,180,1860G→C100.0% 1416.6 379R292P (CGC→CCC) RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (192/187):  ref base (0/0):  total (192/187)

Only 100 of 386 total aligned reads displayed.

GATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGT.GTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACC  >  NC_014034/2180092‑2180283
                                                                                                 ||                                                                                                 
gATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGACCAAGG.CCGG.GGCCGccaa                                                                                               >  1:8707848/1‑98 (MQ=255)
 aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAg                                                                                               >  2:161033/1‑101 (MQ=255)
  tGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGc                                                                                              >  2:2217760/1‑101 (MQ=255)
   gCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCa                                                                                             >  1:834087/1‑101 (MQ=255)
    ccaCCGGGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCAt                                                                                            <  2:1024372/99‑1 (MQ=255)
      aCCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATgg                                                                                          <  2:2053381/101‑1 (MQ=255)
       ccGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGc                                                                                         <  1:4894990/101‑1 (MQ=255)
        cGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCa                                                                                        <  1:3513178/101‑1 (MQ=255)
        cGTGGCCACCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCa                                                                                        <  1:2619486/101‑1 (MQ=255)
         gTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCaa                                                                                       >  2:4961694/1‑101 (MQ=255)
          tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAAc                                                                                      <  2:3075684/101‑1 (MQ=255)
            gccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTg                                                                                    <  2:4583268/101‑1 (MQ=255)
                 ctctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGctct                                                                               <  2:3923183/101‑1 (MQ=255)
                 ctctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGctct                                                                               >  1:8044129/1‑101 (MQ=255)
                 ctctTTCGTGACCAACCCCAAGGACTCGGCCATGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGctct                                                                               <  1:8852590/101‑1 (MQ=255)
                   ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTgg                                                                             >  2:2697014/1‑101 (MQ=255)
                    tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTggg                                                                            <  2:8343876/101‑1 (MQ=255)
                    tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTggg                                                                            <  1:3139954/101‑1 (MQ=255)
                    tttCGTGACCAACCCCAAGGACTCGGCCGTGGCAGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTggg                                                                            >  1:6445304/1‑101 (MQ=255)
                     ttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGc                                                                           <  1:1744285/101‑1 (MQ=255)
                      tCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGcc                                                                          <  1:9135259/101‑1 (MQ=255)
                       cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                         >  1:2741998/1‑101 (MQ=255)
                       cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                         >  1:4644838/1‑101 (MQ=255)
                        gTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTg                                                                        <  2:1160621/101‑1 (MQ=255)
                        gTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTg                                                                        >  2:5369266/1‑101 (MQ=255)
                         tGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTgg                                                                       <  2:4344742/101‑1 (MQ=255)
                          gACCAACCCAAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGa                                                                      >  1:8797963/1‑101 (MQ=255)
                           aCCGACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                                                     >  1:7196000/1‑101 (MQ=255)
                           aCCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                                                     >  1:3900868/1‑101 (MQ=255)
                           aCCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                                                     >  2:706722/1‑101 (MQ=255)
                           aCCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                                                     <  2:8832724/101‑1 (MQ=255)
                            ccAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAc                                                                    >  2:6944964/1‑101 (MQ=255)
                              aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                                  <  1:4467710/101‑2 (MQ=255)
                               aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                                 <  2:8234021/101‑3 (MQ=255)
                               aCCCCAAGGACTCAGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.CCGTCGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                                 <  2:8866581/101‑3 (MQ=255)
                                 cccAACGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcgc                                                               <  1:2953391/101‑5 (MQ=255)
                                  ccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGcc                                                             <  2:1941989/101‑1 (MQ=255)
                                   cAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCa                                                            >  1:1837367/1‑101 (MQ=255)
                                    aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                           <  2:8741199/101‑2 (MQ=255)
                                    aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                           <  2:6577369/101‑2 (MQ=255)
                                     aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                          <  2:8194507/101‑3 (MQ=255)
                                     aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                          >  1:6625488/1‑99 (MQ=255)
                                      ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                         <  2:8049234/101‑4 (MQ=255)
                                       gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGTCTCTGGGCCTGGAA.CCTCGCCattcc                                                        <  2:3676404/101‑5 (MQ=255)
                                       gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctagccattcc                                                         >  2:4498951/1‑91 (MQ=255)
                                       gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                        >  2:3567729/1‑97 (MQ=255)
                                       gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                        <  2:6343378/101‑5 (MQ=255)
                                       gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                        <  1:5369450/101‑5 (MQ=255)
                                        aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                                      >  2:4697687/1‑101 (MQ=255)
                                         cTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                                                     <  1:6851008/101‑1 (MQ=255)
                                          tCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                                                    <  1:7013905/101‑1 (MQ=255)
                                                gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGTCAc                                              >  1:7318894/1‑101 (MQ=255)
                                                gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                              <  2:5713909/101‑1 (MQ=255)
                                                gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                              <  1:7890298/101‑1 (MQ=255)
                                                gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                              <  2:5836003/101‑1 (MQ=255)
                                                  ggCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGc                                            >  1:5763891/1‑101 (MQ=255)
                                                   gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                           >  1:7571742/1‑101 (MQ=255)
                                                   gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                           <  2:3364210/101‑1 (MQ=255)
                                                   gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                           >  1:6778208/1‑101 (MQ=255)
                                                     cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa                                         <  1:3701565/101‑1 (MQ=255)
                                                       aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                       <  1:3383340/101‑2 (MQ=255)
                                                       aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                       <  1:4961854/101‑2 (MQ=255)
                                                       aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                       >  1:1511960/1‑100 (MQ=255)
                                                       aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                       >  1:5127354/1‑100 (MQ=255)
                                                        tCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg                                      >  1:2288038/1‑99 (MQ=255)
                                                        tCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg                                      >  2:7564243/1‑99 (MQ=255)
                                                           tGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                  >  1:1945169/1‑101 (MQ=255)
                                                             gcggcttcgcgcTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg                                <  1:769739/100‑1 (MQ=255)
                                                              cggcttcgcgcTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                               <  1:3605056/101‑1 (MQ=255)
                                                              cggcttcgcgcTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                               <  2:5193521/101‑1 (MQ=255)
                                                               ggcttcgcgcTGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                              >  2:5114613/1‑101 (MQ=255)
                                                                 cttcgcgctGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcgg                            >  1:1066837/7‑101 (MQ=255)
                                                                 cttcgcgctGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAggcgg                            >  2:2911131/7‑101 (MQ=255)
                                                                  ttcgcgctGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcggc                           <  2:2562935/96‑1 (MQ=255)
                                                                       gctGGCGCCGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa                      <  2:854811/101‑1 (MQ=255)
                                                                        ctggcgccGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                     <  1:2217827/95‑1 (MQ=255)
                                                                         tggcgccGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                    >  2:8960799/6‑101 (MQ=255)
                                                                         tggcgccGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                    >  1:7359957/6‑101 (MQ=255)
                                                                         tggcgccGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                    >  1:2857504/6‑101 (MQ=255)
                                                                          ggcgccGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AACAGGACGACGCGGCGCTGAAAt                   >  2:1573877/5‑101 (MQ=255)
                                                                          ggcgccGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                   <  2:6206724/97‑1 (MQ=255)
                                                                           gcgccGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAtt                  <  2:234838/98‑1 (MQ=255)
                                                                            cgccGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc                 <  2:3261393/99‑1 (MQ=255)
                                                                             gccGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcg                <  1:5266325/100‑1 (MQ=255)
                                                                              ccGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgt               >  1:2077459/1‑101 (MQ=255)
                                                                               cGAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc              >  2:5946067/1‑101 (MQ=255)
                                                                                gAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg             >  2:130004/1‑101 (MQ=255)
                                                                                gAACAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg             >  1:4535508/1‑101 (MQ=255)
                                                                                 aaCAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa            <  1:1232836/101‑1 (MQ=255)
                                                                                 aaCAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa            >  2:7885464/1‑101 (MQ=255)
                                                                                   cAAGGCCGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt          <  1:5946302/101‑1 (MQ=255)
                                                                                    aaggccGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATg         <  1:6195973/96‑1 (MQ=255)
                                                                                    aaggccGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAGGCGGCGCTGAAATTCGTCGAATg         >  2:7291952/6‑101 (MQ=255)
                                                                                     aggccGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg        >  1:428556/5‑101 (MQ=255)
                                                                                     aggccGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg        <  2:29807/97‑1 (MQ=255)
                                                                                      ggccGGT.GTCGCCAAGCATGGCAACTGGCTCTCGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATggg       <  2:6753703/98‑1 (MQ=255)
                                                                                        ccGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg     >  1:4152401/2‑101 (MQ=255)
                                                                                        ccGGT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg     <  1:5463631/100‑1 (MQ=255)
                                                                                          ggT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc   >  2:8297588/1‑101 (MQ=255)
                                                                                          ggT.GTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc   >  1:7656689/1‑101 (MQ=255)
                                                                                                 ||                                                                                                 
GATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGT.GTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACC  >  NC_014034/2180092‑2180283

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG