Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	774,619	2 bp→GG	100%	intergenic (‑73/+46)	oppB1 ← / ← oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	774,619	0	C→G	100.0%	45.4	12	intergenic (‑73/+47)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (12/0):  ref base (0/0):  total (12/0)
*	NC_014034	774,620	0	T→G	100.0%	45.3	12	intergenic (‑74/+46)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (12/0):  ref base (0/0):  total (12/0)

CCGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATT  >  NC_014034/774595‑774703                         ||                                                                                      cgcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACg            >  1:1711243/11‑101 (MQ=255) cgcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACg            >  1:5666101/11‑101 (MQ=255) cgcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACg            >  2:3479180/11‑101 (MQ=255)   caggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGtt          >  2:2409717/9‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCCCGTTc         >  1:436300/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc         >  1:3574470/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc         >  1:7872427/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc         >  2:8919022/8‑101 (MQ=255)         cggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTa    >  2:3086055/3‑101 (MQ=255)         cggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGATCCAGTa    >  2:6513074/3‑101 (MQ=255)          ggcgcCCCCCCGCCCGGGTCGGACCGGGCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCCCTTCGCCCCCGACCAGTCGGCCACGTTCCAGCAt   >  2:3287223/2‑101 (MQ=255)          ggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTAt   >  2:1642341/2‑101 (MQ=255)           gcgcCCCCCCGCCCGGGCCGGCCCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTAtt  <  2:3369043/101‑1 (MQ=255)                         ||                                                                                      CCGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATT  >  NC_014034/774595‑774703 Base quality scores:  ATCG  < 3 ≤  ATCG  < 11 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG