Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,795 Δ1 bp 4.2% intergenic (+607/‑324) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,7950C→. 4.2% 30.7 ‑0.9 ‑0.0308intergenic (+607/‑324)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/8):  ref base (153/141):  total (159/149)
Fisher's exact test strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.86e-01

Only 100 of 362 total aligned reads displayed.

GCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGC  >  NC_014034/1395696‑1395895
                                                                                                          |                                                                                                       
 tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                                         <  2:4029717/99‑1 (MQ=2)
  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                                                        >  2:5300578/2‑101 (MQ=2)
  aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                                         >  1:2374453/1‑101 (MQ=2)
  aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAACTg                                                                                                      >  2:4093635/1‑101 (MQ=255)
   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                                                                        <  2:1047468/101‑2 (MQ=2)
   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                                                                        <  1:4525489/101‑2 (MQ=2)
   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                                                                                       <  2:3035377/101‑1 (MQ=2)
   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                                                                     <  1:5223186/101‑1 (MQ=255)
   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                                                                     <  1:295341/101‑1 (MQ=255)
   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                                                                     >  1:2485232/1‑101 (MQ=255)
    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCta                                                                                                      <  1:4774615/101‑2 (MQ=2)
     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaag                                                                                                      >  1:499175/1‑98 (MQ=2)
     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcacc                                                                                                      >  1:972183/1‑98 (MQ=2)
     cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                                                                                     >  1:4591808/1‑99 (MQ=2)
     cGCTCCCCCCGCGGGGGCGGGGATCGAAACC.GGTACGGATCTG..GGGTAAACGG..AAGGGCT.C.GAGTGGCTCCCCACGCGGGGGGGGGGGGCGAAAcaagcag                                                                                                   >  2:1203382/1‑95 (MQ=12)
     cGCGCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGACCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                                                                                     <  2:4955516/101‑3 (MQ=2)
      gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                                                                    >  1:1576866/1‑98 (MQ=2)
      gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                                                                    <  1:3168596/101‑4 (MQ=2)
      gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCCGGGGCGTGGATCGAAACCtatg                                                                                                    >  2:724596/1‑98 (MQ=2)
       cTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatga                                                                                                   <  2:2804978/101‑5 (MQ=2)
        tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcacccgg                                                                                                   >  2:4887347/1‑95 (MQ=2)
        tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg.gcgg                                                                                               >  2:3778981/1‑100 (MQ=255)
        tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGt                                                                                               <  2:2913854/101‑1 (MQ=255)
        tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGt                                                                                               >  1:3206654/1‑101 (MQ=255)
        cccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccgg                                                                                                   <  1:3059185/100‑7 (MQ=0)
         ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                                                                                  <  2:1826228/101‑8 (MQ=0)
         ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTg                                                                                              >  1:4384581/1‑101 (MQ=255)
          cccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGa                                                                                             <  1:2760755/101‑1 (MQ=255)
          cccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGa                                                                                             <  2:5397368/101‑1 (MQ=255)
            cccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgcc                                                                                               <  2:1499589/101‑11 (MQ=2)
            cccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgcc                                                                                               <  1:3957830/101‑11 (MQ=2)
            cccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGt                                                                                           >  2:2233704/1‑101 (MQ=255)
             ccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGtt                                                                                          >  2:882449/1‑101 (MQ=255)
                cgGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGcc                                                                                       <  1:5083518/101‑1 (MQ=255)
                   gggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGt                                                                                    <  2:690836/101‑1 (MQ=255)
                   gggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGt                                                                                    <  2:4623966/101‑1 (MQ=255)
                    ggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTg                                                                                   >  2:1086876/1‑101 (MQ=255)
                    ggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTg                                                                                   <  2:5721227/101‑1 (MQ=255)
                     gCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGa                                                                                  >  2:5548195/1‑101 (MQ=255)
                      cGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGAt                                                                                 >  1:3686818/1‑101 (MQ=255)
                      cGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGAt                                                                                 <  1:1524499/101‑1 (MQ=255)
                        tGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAg                                                                               >  1:1157957/1‑101 (MQ=255)
                          gATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.c                                                                            <  1:5361798/101‑1 (MQ=255)
                          gATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.c                                                                            >  2:1972324/1‑101 (MQ=255)
                              gAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCg                                                                        >  2:3267762/1‑101 (MQ=255)
                                aaCT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTg                                                                      >  1:3271256/1‑101 (MQ=255)
                                 aCT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTgg                                                                     >  1:5663746/1‑101 (MQ=255)
                                  cT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGa                                                                    <  2:2302331/101‑1 (MQ=255)
                                   t.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.c                                                                  <  1:136234/101‑1 (MQ=255)
                                     ggTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.Cg                                                                 <  1:5763400/101‑1 (MQ=255)
                                       tATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTc                                                               >  1:3405087/1‑101 (MQ=255)
                                       tATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTc                                                               <  2:79502/101‑1 (MQ=255)
                                        aTGGATCTG..GCTCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCg                                                              <  2:4717644/101‑1 (MQ=255)
                                        aTGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCg                                                              <  2:3637207/101‑1 (MQ=255)
                                          ggATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCt                                                            <  2:3295916/101‑1 (MQ=255)
                                              cTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                                                        >  2:5761877/1‑101 (MQ=255)
                                              cTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                                                        <  1:4833207/101‑1 (MQ=255)
                                              cTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                                                        <  1:1736450/101‑1 (MQ=255)
                                              cTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                                                        <  2:3701831/101‑1 (MQ=255)
                                               tG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTccccc                                                       >  1:5510495/1‑101 (MQ=255)
                                                     gcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcccg                                                   >  1:3373228/1‑101 (MQ=255)
                                                      cAAAGGG..AAAGTCT.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                                                  <  2:5017139/101‑1 (MQ=255)
                                                      cAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                                                  >  2:3450623/1‑101 (MQ=255)
                                                      cAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                                                  >  2:421550/1‑101 (MQ=255)
                                                       aaaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggg                                                 >  2:1386939/1‑101 (MQ=255)
                                                        aaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgggg                                                <  2:3139127/101‑1 (MQ=255)
                                                           gg..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCg                                             <  1:1716095/101‑1 (MQ=255)
                                                            g..AAAGTCT.G.GCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGt                                            <  1:4017808/101‑1 (MQ=255)
                                                               aaaGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTg                                           <  2:3368930/101‑1 (MQ=255)
                                                               aaaGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTg                                           >  1:1023535/1‑101 (MQ=255)
                                                                aaGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.GGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg                                          >  1:4926827/1‑101 (MQ=255)
                                                                aaGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg                                          >  1:3121786/1‑101 (MQ=255)
                                                                 ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                            <  2:4506569/92‑1 (MQ=0)
                                                                 ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                            >  1:5592627/10‑101 (MQ=0)
                                                                  gTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                        <  1:2176042/101‑1 (MQ=255)
                                                                  gTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                        <  1:664766/101‑1 (MQ=255)
                                                                   tCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                       <  2:5663451/101‑1 (MQ=255)
                                                                   acccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGCgg                                          >  2:2528788/8‑101 (MQ=0)
                                                                    cT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                      <  1:1243645/101‑1 (MQ=255)
                                                                             cgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgc                               <  1:4695201/101‑1 (MQ=255)
                                                                                tCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgcc                            >  1:4303772/1‑101 (MQ=255)
                                                                                tCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgcc                            <  2:1564768/101‑1 (MQ=255)
                                                                                tCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgcc                            >  2:3192613/1‑101 (MQ=255)
                                                                                  cccccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgccgc                          >  2:3071201/1‑101 (MQ=255)
                                                                                     ccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                       >  2:2376971/1‑101 (MQ=255)
                                                                                     ccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                       >  1:1645119/1‑101 (MQ=255)
                                                                                       gcgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATc                     <  1:1972435/101‑1 (MQ=255)
                                                                                        cgGGGCCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGCGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAtgg                    >  1:3892749/1‑99 (MQ=255)
                                                                                         gggggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGc                   >  1:733456/1‑101 (MQ=255)
                                                                                          ggggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCa                  >  2:5189379/1‑101 (MQ=255)
                                                                                             gCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc               <  1:2686957/101‑1 (MQ=255)
                                                                                             gCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc               >  1:1075071/1‑101 (MQ=255)
                                                                                              cGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg              >  1:2281266/1‑101 (MQ=255)
                                                                                              cGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg              <  1:3816402/101‑1 (MQ=255)
                                                                                              cGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg              <  2:4351790/101‑1 (MQ=255)
                                                                                               gTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg             >  2:3844319/1‑101 (MQ=255)
                                                                                                tGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGt            >  1:4145379/1‑101 (MQ=255)
                                                                                                   aTCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGa         <  2:1710515/101‑1 (MQ=255)
                                                                                                       aaaCCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAgcgc     >  2:2620527/1‑101 (MQ=255)
                                                                                                          ccTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGcgccgc  <  2:2111163/101‑1 (MQ=255)
                                                                                                          |                                                                                                       
GCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGC  >  NC_014034/1395696‑1395895

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG