Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,398,002 Δ1 bp 1.6% intergenic (+306/+1592) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,398,0020C→. 1.6% 4.2 0.0 ‑0.4254intergenic (+306/+1592)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (133/116):  total (136/118)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.74e-01

Only 100 of 287 total aligned reads displayed.

AGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGC  >  NC_014034/1397902‑1398102
                                                                                                    |                                                                                                       
aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAc                                                                                                         >  2:3840550/1‑101 (MQ=255)
 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                                                        <  1:1507698/101‑1 (MQ=255)
  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccg                                                                                                       <  2:3376302/101‑1 (MQ=255)
    gCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccgcc                                                                                                     <  1:4425845/101‑1 (MQ=255)
    gCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccgcc                                                                                                     <  1:2863934/101‑1 (MQ=255)
     cTCCCCCCGCGGGGGCGTGGTTCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccgccg                                                                                                    >  2:1583561/1‑101 (MQ=255)
     cTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccgccg                                                                                                    <  1:341574/101‑1 (MQ=255)
         ccccGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCa                                                                                                >  2:1815596/1‑101 (MQ=255)
            cgcgGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCaaaa                                                                                             <  1:4365864/101‑1 (MQ=255)
             gcgGGGGCGTGGATCGAATCTCTATGGGAGGCTAGGGAGTCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGGGGATCTAAACCGCCGCGCaaaaa                                                                                            <  2:977202/101‑1 (MQ=38)
             gcgGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCaaaaa                                                                                            >  2:2720047/1‑101 (MQ=255)
             gcgGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCaaaaa                                                                                            >  2:4833621/1‑101 (MQ=255)
               gggggCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGc                                                                                          >  2:2088068/1‑101 (MQ=255)
                ggggCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCCGCGCAAAAAGcc                                                                                         >  2:4482926/1‑101 (MQ=255)
                ggggCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGcc                                                                                         >  2:1129772/1‑101 (MQ=255)
                    cattgATCGAAACTCTATGGGAGGCTAGGGAGCCTACGCGCCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACCGCCGAGCAAAAAGCCCGcc                                                                                     <  2:5711432/97‑1 (MQ=37)
                    cGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGcc                                                                                     >  2:2397043/1‑101 (MQ=255)
                     gTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCa                                                                                    >  1:5667467/1‑101 (MQ=255)
                     gTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCa                                                                                    >  2:4807936/1‑101 (MQ=255)
                     gTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCa                                                                                    <  1:3072198/101‑1 (MQ=255)
                      tGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCaa                                                                                   >  2:2784871/1‑101 (MQ=255)
                      tGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCaa                                                                                   >  1:4070669/1‑101 (MQ=255)
                         aTCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCaagaa                                                                                >  2:5446782/1‑101 (MQ=255)
                         aTCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCaagaa                                                                                >  1:120063/1‑101 (MQ=255)
                          tCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCaagaag                                                                               <  1:4256939/101‑1 (MQ=255)
                            gAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCg                                                                             >  2:5597232/1‑101 (MQ=255)
                            gAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCg                                                                             >  2:4770001/1‑101 (MQ=255)
                             gatctctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCgg                                                                            <  1:1969606/98‑1 (MQ=255)
                             aaaCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCgg                                                                            <  1:4031122/101‑1 (MQ=255)
                              aaCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..a                                                                         >  2:4015382/1‑101 (MQ=255)
                               aCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..Ag                                                                        <  1:5101804/101‑1 (MQ=255)
                                ctctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGc                                                                       <  2:5360576/101‑1 (MQ=255)
                                 tctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCa                                                                      >  2:2542963/1‑101 (MQ=255)
                                 tctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCa                                                                      >  2:4156431/1‑101 (MQ=255)
                                 tctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCa                                                                      >  2:4422851/1‑101 (MQ=255)
                                  ctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCa.a                                                                    <  1:2801143/101‑1 (MQ=255)
                                   tATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.Ag                                                                   <  2:1197715/101‑1 (MQ=255)
                                   tATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAACCCGCCAAGAAGCGG..AGCA.Ag                                                                   >  1:1122748/1‑101 (MQ=255)
                                    aTGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGt                                                                  >  1:854999/1‑101 (MQ=255)
                                    aTGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGt                                                                  <  1:1799828/101‑1 (MQ=255)
                                    aTGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGt                                                                  <  2:5669236/101‑1 (MQ=255)
                                     tGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTc                                                                 <  1:4722982/101‑1 (MQ=255)
                                      gggaggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCg                                                                >  1:3647061/1‑101 (MQ=255)
                                      gggaggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCg                                                                <  2:5559986/101‑1 (MQ=255)
                                          ggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcc                                                            <  1:3890589/101‑1 (MQ=255)
                                             tagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTccccc                                                         <  2:2011962/101‑1 (MQ=255)
                                              agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcccccc                                                        >  1:2310153/1‑101 (MQ=255)
                                              agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcccccc                                                        <  1:88953/101‑1 (MQ=255)
                                              agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcccccc                                                        <  2:584119/101‑1 (MQ=255)
                                              agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcccccc                                                        >  1:766962/1‑101 (MQ=255)
                                               gggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCcg                                                       >  2:1942128/1‑101 (MQ=255)
                                                ggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCcgc                                                      >  2:4655791/1‑101 (MQ=255)
                                                 gaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCcgcg                                                     <  2:343126/101‑1 (MQ=255)
                                                  gggctaCGCCCCTTCAAAAGTCGCTCCCCCCGCGGGGCCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCgg                                                    <  2:1367250/100‑1 (MQ=255)
                                                  aggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCgg                                                    >  2:4390677/1‑101 (MQ=255)
                                                  aggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCgg                                                    <  1:2172995/101‑1 (MQ=255)
                                                      taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGc                                                >  2:5519230/1‑101 (MQ=255)
                                                      taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGc                                                <  1:1583655/101‑1 (MQ=255)
                                                      taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGc                                                >  1:751215/1‑101 (MQ=255)
                                                            ccTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGAt                                          <  1:4828444/101‑1 (MQ=255)
                                                            ccTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGAt                                          >  2:312/1‑101 (MQ=255)
                                                             cTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATc                                         >  2:3743021/1‑101 (MQ=255)
                                                             cTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATc                                         <  2:5650296/101‑1 (MQ=255)
                                                               tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.CGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                         >  1:1775134/7‑101 (MQ=2)
                                                               tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.CGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                         <  1:2512478/95‑1 (MQ=2)
                                                               tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.CGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                         <  2:3545145/95‑1 (MQ=2)
                                                                cAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTa                                      <  2:5221852/101‑1 (MQ=255)
                                                                cAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTa                                      >  2:5140408/1‑101 (MQ=255)
                                                                 aaaaGCCGCTCCCCCCGCGGGGGCGTGGATCAAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTaa                                     <  1:1062868/101‑1 (MQ=255)
                                                                   aaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGc                                   <  1:4656067/101‑1 (MQ=255)
                                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCggg                                >  2:1026179/1‑100 (MQ=255)
                                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..ATCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTg                               >  1:2579662/1‑101 (MQ=255)
                                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTg                               >  2:796321/1‑101 (MQ=255)
                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGc                              >  2:2145595/1‑101 (MQ=255)
                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGGGc                              >  1:2446368/1‑101 (MQ=255)
                                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCa                             >  1:3250909/1‑101 (MQ=255)
                                                                          tCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCAt                            >  1:3967480/1‑101 (MQ=255)
                                                                           ccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATc                           <  2:3504729/101‑1 (MQ=255)
                                                                             ccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCgg                         >  1:2646138/1‑101 (MQ=255)
                                                                              cccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGc                        >  2:5083289/1‑101 (MQ=255)
                                                                                cgcgGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGccc                      <  1:5136228/101‑1 (MQ=255)
                                                                                 gcgGGGGCGTGGATCGAACCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCg                     <  1:1024253/101‑1 (MQ=255)
                                                                                   gggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGAt                   <  1:5403303/101‑1 (MQ=255)
                                                                                    ggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATa                  <  1:5519522/101‑1 (MQ=255)
                                                                                     gggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATaa                 <  2:3025197/101‑1 (MQ=255)
                                                                                         gTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAccc             <  2:1859343/101‑1 (MQ=255)
                                                                                          tGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAcccc            <  1:5597524/101‑1 (MQ=255)
                                                                                           ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg           <  1:1815706/101‑1 (MQ=255)
                                                                                           ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg           >  1:67425/1‑101 (MQ=255)
                                                                                           ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg           <  1:3512693/101‑1 (MQ=255)
                                                                                            gATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGa          >  1:3171510/1‑101 (MQ=255)
                                                                                                gAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAtttt      <  1:2088185/101‑1 (MQ=255)
                                                                                                gAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAtttt      <  2:221605/101‑1 (MQ=255)
                                                                                                gAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACACCGAtttt      <  1:4218316/101‑1 (MQ=255)
                                                                                                gAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGGAAGCGGTGCATCGGCCCGATAAAACCCGAtttt      >  1:4931815/1‑101 (MQ=255)
                                                                                                 aaaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGCTTTTc     >  2:3320016/1‑101 (MQ=255)
                                                                                                  aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.ATTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAttttag    <  2:2962563/101‑3 (MQ=255)
                                                                                                  aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg    >  1:5224609/1‑101 (MQ=255)
                                                                                                  aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg    <  2:4691261/101‑1 (MQ=255)
                                                                                                    ccgccgCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGc  >  1:2115626/1‑101 (MQ=255)
                                                                                                    |                                                                                                       
AGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGC  >  NC_014034/1397902‑1398102

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG