Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,585,929 Δ1 bp 1.4% coding (492/1095 nt) RCAP_rcc01465 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,585,9290G→. 1.4% 4.1 0.0 ‑0.2278coding (492/1095 nt)RCAP_rcc01465hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (137/137):  total (139/139)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.50e-01

Only 100 of 310 total aligned reads displayed.

CCTTGCCGGCAATCGAGAAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGCGCCAGCACCTTGCGGGTGA  >  NC_014034/1585829‑1586029
                                                                                                    |                                                                                                    
 cTTGCCGGCAATCGAGAAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATAACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTgg                                                                                                     >  1:4030790/1‑101 (MQ=255)
  ttGCCGGCAATCGAGAAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTggg                                                                                                    <  2:4529472/101‑1 (MQ=255)
   tGCCGGCAATCGAGAAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTgggg                                                                                                   >  2:4457347/1‑101 (MQ=255)
   tGCCGGCAATCGAGAAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTgggg                                                                                                   <  1:3788035/101‑1 (MQ=255)
    gCCGGCAATCGAGAAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTTCGGCGACATCACATAGCGCGTTTCCTGCAGGATCAGATCCTTGCTggggg                                                                                                  >  1:4705753/1‑101 (MQ=255)
    gCCGGCAATCGAGAAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTggggg                                                                                                  >  2:4493062/1‑101 (MQ=255)
       ggCAATCGAGAAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTgggggggg                                                                                               >  2:2526025/1‑101 (MQ=255)
         cAATCGAGAAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCa                                                                                             <  2:3184791/101‑1 (MQ=255)
             cGAGAAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCAGCAGGATCAGATCCTTGCTGGGGGGGGCATCGa                                                                                         >  1:4937983/1‑101 (MQ=255)
              gagaAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGAc                                                                                        <  1:1722873/101‑1 (MQ=255)
              gagaAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGAc                                                                                        <  2:4153988/101‑1 (MQ=255)
              gagaAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGAc                                                                                        <  2:5795804/101‑1 (MQ=255)
               agaagaTCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGCCATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACg                                                                                       >  1:2174827/1‑101 (MQ=255)
                gaagaTCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGCCGt                                                                                      >  2:1521083/1‑101 (MQ=255)
                   gaTCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCtt                                                                                   <  2:5125333/101‑1 (MQ=255)
                     tCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTc                                                                                 <  2:2783456/101‑1 (MQ=255)
                      cGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCg                                                                                >  1:4377458/1‑101 (MQ=255)
                      cGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCg                                                                                <  2:4977964/101‑1 (MQ=255)
                       gTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCgg                                                                               <  1:3869425/101‑1 (MQ=255)
                        tCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGc                                                                              <  1:3110486/101‑1 (MQ=255)
                         ccGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCa                                                                             <  2:4311084/101‑1 (MQ=255)
                          cGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAg                                                                            >  2:162950/1‑101 (MQ=255)
                            gTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGt                                                                          >  1:5443573/1‑101 (MQ=255)
                               cAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCa                                                                       <  2:224207/101‑1 (MQ=255)
                               cAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGGCCa                                                                       >  1:4396778/1‑101 (MQ=255)
                               cAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGGCCa                                                                       >  2:2383292/1‑101 (MQ=255)
                                agagACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCAt                                                                      >  2:4619929/1‑101 (MQ=255)
                                agagACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGGCCAt                                                                      >  1:5499782/1‑101 (MQ=255)
                                 gagaCGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCGGGGCCATg                                                                     >  1:4292770/1‑101 (MQ=255)
                                 gagaCGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATg                                                                     <  1:2777355/101‑1 (MQ=255)
                                  agaCGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGt                                                                    >  2:1130524/1‑101 (MQ=255)
                                  agaCGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGt                                                                    <  1:848437/101‑1 (MQ=255)
                                   gaCGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTa                                                                   <  1:1108172/101‑1 (MQ=255)
                                   gaCGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTa                                                                   <  1:4985678/101‑1 (MQ=255)
                                     cGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTaaa                                                                 <  2:688144/101‑1 (MQ=255)
                                     cGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTaaa                                                                 <  1:5796693/101‑1 (MQ=255)
                                      ggCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGGCCCTGTAAAg                                                                >  2:4923145/1‑101 (MQ=255)
                                       gCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGc                                                               <  2:4060690/101‑1 (MQ=255)
                                         ttGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGa                                                             >  1:4653490/1‑101 (MQ=255)
                                            cgcAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCT.GGGGGGGCATCGACGTCTTTCGGCAGGTCCACGTAAAACGAGGGa                                                         >  2:2197050/1‑101 (MQ=255)
                                              cAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAgggca                                                        >  2:1027223/1‑99 (MQ=255)
                                              cAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGAc                                                        <  1:5176592/101‑1 (MQ=255)
                                               aGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACa                                                       <  2:2343757/101‑1 (MQ=255)
                                                  tatTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGcc                                                    >  1:1983921/1‑101 (MQ=255)
                                                   atTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCg                                                   <  1:3263112/101‑1 (MQ=255)
                                                      gcggcgACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTc                                                <  2:1296855/101‑1 (MQ=255)
                                                       cggcgACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTca                                               <  2:261707/101‑1 (MQ=255)
                                                       cggcgACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCCACGTCGTTCGGCAGGTCCATGTAAAGCGAGGGACAGCAGGTca                                               >  2:1367716/1‑101 (MQ=255)
                                                        ggcgACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTcac                                              <  2:4388768/101‑1 (MQ=255)
                                                          cgACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTcacca                                            >  1:2599675/1‑101 (MQ=255)
                                                            aCATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGGCCATGTAAAGCGAGGGACAGCCGGGCACCACg                                          >  2:2885323/1‑101 (MQ=255)
                                                             cATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACgg                                         >  2:1247410/1‑101 (MQ=255)
                                                             cATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACgg                                         <  2:5560151/101‑1 (MQ=255)
                                                              aTCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAACCGAGGGACAGCCGGTCACCACGGt                                        >  2:3452516/1‑101 (MQ=255)
                                                               tCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTa                                       <  1:4620205/101‑1 (MQ=255)
                                                               tCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTa                                       >  1:2456384/1‑101 (MQ=255)
                                                               tCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTa                                       >  1:1179451/1‑101 (MQ=255)
                                                                cacaTAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTGTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTAt                                      >  1:4868800/1‑101 (MQ=255)
                                                                 acaTAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTAtt                                     >  1:176200/1‑101 (MQ=255)
                                                                 acaTAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTAtt                                     <  2:1452628/101‑1 (MQ=255)
                                                                  caTAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCCCGGTAttt                                    >  1:1520165/1‑100 (MQ=255)
                                                                  caTAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGGCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATtg                                    >  1:2516069/1‑101 (MQ=255)
                                                                   aTAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATtgt                                   >  1:2971869/1‑101 (MQ=255)
                                                                    tAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATtgtg                                  <  1:2531924/101‑1 (MQ=255)
                                                                    tAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATtgtg                                  >  2:3296377/1‑101 (MQ=255)
                                                                     aGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTgg                                 <  1:4240368/101‑1 (MQ=255)
                                                                      gcgcGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGGCCATGTAAAGAGAGGGACAGCCGGCCACCACGGGATTGTGGc                                >  1:3075500/1‑101 (MQ=255)
                                                                       cgcgTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCa                               <  2:4222829/101‑1 (MQ=255)
                                                                        gcgTTCCCTGCAGGATCAGATCCTTGCT.GGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGc                             <  1:2381621/101‑1 (MQ=255)
                                                                           ttCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGccc                           <  1:3583668/101‑1 (MQ=255)
                                                                             cccTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAg                         <  1:162970/101‑1 (MQ=255)
                                                                              ccTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGa                        <  1:4493334/101‑1 (MQ=255)
                                                                              ccTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGa                        <  1:338880/101‑1 (MQ=255)
                                                                              ccTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGa                        <  1:2798369/101‑1 (MQ=255)
                                                                               cTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGAt                       <  1:5672444/101‑1 (MQ=255)
                                                                               cTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGGCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGGCCAGAt                       >  1:892556/1‑101 (MQ=255)
                                                                                 gCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATgg                     >  2:3778555/1‑100 (MQ=255)
                                                                                 gCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATgc                     >  2:1568179/1‑101 (MQ=255)
                                                                                 gCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATgc                     <  1:1892197/101‑1 (MQ=255)
                                                                                 gCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGGCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATgc                     >  1:746982/1‑101 (MQ=255)
                                                                                    ggATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGGGcc                  >  1:4066582/1‑101 (MQ=255)
                                                                                     gATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGCGCCa                 <  1:1393973/101‑1 (MQ=255)
                                                                                     gATCACATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGCGCCa                 <  2:1756421/101‑1 (MQ=255)
                                                                                      aTCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGGGCCac                >  2:783808/1‑100 (MQ=255)
                                                                                      aTCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGCGCCAg                <  2:1175604/101‑1 (MQ=255)
                                                                                        cAGATCCTTGCTGGGGGGGGCATCGACGCCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGGATTCTGGAACCCAAGATACGCCAGCa              >  2:3626843/1‑101 (MQ=255)
                                                                                         aGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCCGATGCGCCAGCAc             >  1:1039503/1‑101 (MQ=255)
                                                                                           aTCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGGCCATGTAAAGCGAGGGACAGCCGGCAACCACGGTATTGCGGCAGCCCAGATGCGCCCGCACCt           >  2:2925891/1‑101 (MQ=255)
                                                                                            tCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGCGCCAGGACCtt          >  2:2698406/1‑101 (MQ=255)
                                                                                            tCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGCGCCAGCACCtt          <  2:281881/101‑1 (MQ=255)
                                                                                            tCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGCGCCAGCACCtt          >  2:4850237/1‑101 (MQ=255)
                                                                                            tCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGCGCCAGCACCtt          <  1:2678425/101‑1 (MQ=255)
                                                                                            tCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGGATTGTGGCAGGCCAGATGCGCCAGCACCtt          >  2:3996627/1‑101 (MQ=255)
                                                                                                tGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGCGCCAGCACCTTGCgg      >  1:2903135/1‑101 (MQ=255)
                                                                                                tGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGAATTGTGGCAGCCCCGATGCGCCAGCCCCTTGCgg      >  1:1482825/1‑101 (MQ=255)
                                                                                                tGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAACGCAGGACAACCCGGCACCAAGGTATTGTGGCAGCCCAAATGCGCCCGCACCCTGCgg      >  2:5257668/1‑101 (MQ=255)
                                                                                                  cTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGCGCCAGCACCTTGCGGGt    <  1:1304094/101‑1 (MQ=255)
                                                                                                   tGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGGCCAGATGAGCCAGCACCTTGCGGGTg   >  2:1903397/1‑101 (MQ=255)
                                                                                                   tGGGGGGGGCATCGACGTCTTTCGGCAGGGCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCTGCTACGCCCACCCCTTGCggggg   >  1:2291256/1‑99 (MQ=255)
                                                                                                   gggggggggCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGCGCCAGCACCTTGCGGGTg   <  2:5443283/100‑1 (MQ=255)
                                                                                                    |                                                                                                    
CCTTGCCGGCAATCGAGAAGATCGTCCGGTCCAGAGACGGCTTGCGCAGATATTGCGGCGACATCACATAGCGCGTTCCCTGCAGGATCAGATCCTTGCTGGGGGGGGCATCGACGTCTTTCGGCAGGTCCATGTAAAGCGAGGGACAGCCGGTCACCACGGTATTGTGGCAGCCCAGATGCGCCAGCACCTTGCGGGTGA  >  NC_014034/1585829‑1586029

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG