Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,607,942 Δ1 bp 100% intergenic (+212/‑135) RCAP_rcc01484 → / → murD hypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,607,9420T→.100.0% 714.7 163intergenic (+212/‑135)RCAP_rcc01484/murDhypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  new base (58/104):  ref base (0/0):  total (58/105)

Only 100 of 187 total aligned reads displayed.

GCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGGCCGCGCGGC  >  NC_014034/1607839‑1608037
                                                                                                       |                                                                                               
gCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGGGGCGGCCCGGGG.ATGCGGGC.AGACCGGGGC.GACCCCCGCCCGTCt                                                                                                 >  1:4223004/1‑101 (MQ=255)
gCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCt                                                                                                 >  2:1158474/1‑101 (MQ=255)
gCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCt                                                                                                 <  2:3699340/101‑1 (MQ=255)
  ccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtcc                                                                                               <  1:4698036/101‑3 (MQ=255)
    gCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTCTCcca                                                                                             >  1:337134/1‑100 (MQ=255)
    gCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.CGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtccga                                                                                             >  2:1831871/1‑97 (MQ=255)
    cccttGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtccga                                                                                             <  2:4222744/98‑5 (MQ=255)
     cATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAc                                                                                           <  1:3861207/101‑1 (MQ=255)
     cATTGTGGCCGCCCCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAc                                                                                           <  1:3615135/101‑1 (MQ=255)
      aTTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTC.TCCGAcc                                                                                          >  2:3200041/1‑101 (MQ=255)
        tgtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtt                                                                                        <  2:5032424/101‑1 (MQ=255)
        tgtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtt                                                                                        <  2:443031/101‑1 (MQ=255)
         gtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCttt                                                                                       <  1:5314087/101‑1 (MQ=255)
          tgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTAAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtttt                                                                                      <  2:5518148/101‑1 (MQ=255)
            gccgccGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTcc                                                                                    >  1:5237831/1‑101 (MQ=255)
             ccgccgCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTccc                                                                                   <  1:2534568/101‑1 (MQ=255)
             ccgccgCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTccc                                                                                   <  2:4310560/101‑1 (MQ=255)
               gccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAg                                                                                 >  1:336171/1‑101 (MQ=255)
                ccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.CACCGCCGCCTGTC.TCCGACCTTTTCCCacc                                                                                >  1:899756/1‑99 (MQ=255)
                 cgccCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGGGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGACGCCTGTC.TCCGACCTTTTCCCAGCg                                                                               <  1:5375069/101‑1 (MQ=255)
                  gcgcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TNCGACCTTTTCCCCGCgg                                                                              >  2:2691937/1‑101 (MQ=255)
                   cgcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGa                                                                             <  1:1530436/101‑1 (MQ=255)
                    gcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGAt                                                                            >  2:5143534/1‑101 (MQ=255)
                        cAGACCCCGCGCCCCGGCCCGTCAAGGATGGCGGCGTCCC.GGGAATGCGGGCG.GACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                                        <  2:3311291/101‑1 (MQ=255)
                        cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                                        <  1:3200230/101‑1 (MQ=255)
                        cAGAACGCGCACCCCGGCCCCTCAAGGATGCCGGCGGCCC.GGGACTGCAGGC.AGACCTGGCC.GACCCCCGCCAGACTACCGCCCTTTCccctcgtgtcccc                                                                         >  2:4991434/1‑91 (MQ=255)
                         aGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                                                       <  1:2211518/101‑1 (MQ=255)
                         aGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                                                       >  1:3461683/1‑101 (MQ=255)
                          gAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgcg                                                                      >  1:2303559/1‑101 (MQ=255)
                          gAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgcg                                                                      >  2:2979270/1‑101 (MQ=255)
                          gAACGCGCGCCCCGGCCCCTAAAGGATGGCGGC.GCCCGGGAAAGGCGTGC.AGTCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgcg                                                                      <  2:1345177/101‑1 (MQ=255)
                          gAACGCCCGCCCCGGCCCCTCAAGGATGGCGGCGTCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgcg                                                                      <  1:2245451/101‑1 (MQ=255)
                             cgcgcgCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACg                                                                   <  1:610851/101‑1 (MQ=255)
                             cgcgcgCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACg                                                                   <  2:3515580/101‑1 (MQ=255)
                               cgcgcCCCGGCCCCTCAAGGATGGCGGCGGCGC.GGGAATGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACggg                                                                 <  1:1881856/101‑1 (MQ=255)
                               cgcgcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACggg                                                                 <  1:5658891/101‑1 (MQ=255)
                                gcgcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACgggg                                                                >  2:1230606/1‑101 (MQ=255)
                                 cgcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGGCggggg                                                               >  1:3473449/1‑100 (MQ=255)
                                 ccccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGt                                                               <  1:2801830/99‑1 (MQ=255)
                                  cccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTg                                                              <  1:1114082/100‑1 (MQ=255)
                                   ccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGc                                                             <  1:2328484/101‑1 (MQ=255)
                                     ccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGa                                                           >  1:4696945/1‑101 (MQ=255)
                                      cGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGAc                                                          >  2:2046264/1‑101 (MQ=255)
                                      cGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGAc                                                          <  2:4154972/101‑1 (MQ=255)
                                       ggCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACg                                                         >  1:5035868/1‑101 (MQ=255)
                                       ggCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACg                                                         >  2:4447731/1‑101 (MQ=255)
                                         ccccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGcc                                                       <  2:5307257/101‑1 (MQ=255)
                                          cccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCt                                                      >  1:4493886/1‑101 (MQ=255)
                                           ccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtg                                                     <  2:2511/101‑2 (MQ=255)
                                           ccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtg                                                     <  1:2078597/101‑2 (MQ=255)
                                           ccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtg                                                     <  1:2456869/101‑2 (MQ=255)
                                            cTCAGGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgc                                                    <  2:5344976/101‑3 (MQ=255)
                                             tCAAGGATGGCGGCGGCCC.GGGAATGCGGGCG.GACCGGGCC.GACCGCCGCCTG.CCTCCGGCCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcg                                                   <  2:942211/101‑4 (MQ=255)
                                             tCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcg                                                   <  1:5335445/101‑4 (MQ=255)
                                             tCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcg                                                   <  2:2460790/101‑4 (MQ=255)
                                              cAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcgg                                                  >  1:1007920/1‑97 (MQ=255)
                                              cAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGACGCCtgcgg                                                  >  1:1230892/1‑97 (MQ=255)
                                               aaGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.Tgcggc                                                >  1:2866214/1‑101 (MQ=255)
                                               aaGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGAAGCCtcgggc                                                 >  2:1103977/1‑96 (MQ=255)
                                               aaGGAGGGGGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.Tgcggc                                                <  2:1988621/101‑1 (MQ=255)
                                                aGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGGC.Tgcggcg                                               >  2:4968886/1‑101 (MQ=255)
                                                  gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc                                             <  2:652253/101‑1 (MQ=255)
                                                   aTGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgccc                                            >  2:1677447/1‑100 (MQ=255)
                                                    tGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGCGCGACGCC.TGAGGcgccgc                                           >  1:793200/1‑101 (MQ=255)
                                                      gcggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGccc                                         <  1:404067/101‑1 (MQ=255)
                                                       cggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg                                        <  1:310904/101‑1 (MQ=255)
                                                        ggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCgc                                       >  2:4641724/1‑100 (MQ=255)
                                                             gcccgggAATGCGGGC.ATACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAgg                                   <  2:3068151/97‑1 (MQ=255)
                                                              cccgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGCCGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAggg                                  >  1:1490507/4‑101 (MQ=255)
                                                                 gggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGaaaacgggc                               >  1:1568104/1‑95 (MQ=255)
                                                                  ggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGc                              <  2:3353610/101‑1 (MQ=255)
                                                                   gAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCt                             <  1:1722106/101‑1 (MQ=255)
                                                                    cttccgatctagaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCt                             <  2:5432623/91‑1 (MQ=255)
                                                                    aaTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGaacaacgggcgg                            >  2:313914/1‑91 (MQ=255)
                                                                    aaTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTg                            <  2:388193/101‑1 (MQ=255)
                                                                     aggCGGGC.AGACC.GGCCCGACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTgc                           <  2:3087211/99‑1 (MQ=255)
                                                                     aTGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTgc                           <  1:5534670/101‑1 (MQ=255)
                                                                      ggCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTgcg                          <  2:4507727/100‑1 (MQ=255)
                                                                           ggcagaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGcc                      <  1:2489093/98‑1 (MQ=255)
                                                                           ggcagaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.GGCGGCGCCGCCCGAAAGGGGGGCTGCGCGcc                      >  2:3798266/4‑101 (MQ=255)
                                                                              agaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCtagatcggaag                   >  1:5432913/1‑91 (MQ=255)
                                                                                 ccGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCctc                <  2:822445/101‑1 (MQ=255)
                                                                                  cGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCctct               <  1:2637146/101‑1 (MQ=255)
                                                                                   gggCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCtt              <  1:3172230/101‑1 (MQ=255)
                                                                                   cgggccgcccGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCctct               <  1:176906/93‑1 (MQ=255)
                                                                                    gggccgcccGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCtt              <  1:758370/94‑1 (MQ=255)
                                                                                     ggccgACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTg             <  1:1290835/97‑1 (MQ=255)
                                                                                         gcccGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTcc         <  2:4539843/99‑1 (MQ=38)
                                                                                         gACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTcc         <  1:1158555/101‑1 (MQ=38)
                                                                                         gACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCACGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTcc         >  1:4991682/1‑101 (MQ=38)
                                                                                          cccGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTCcg        <  1:1220822/100‑1 (MQ=38)
                                                                                           ccGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTCcgc       <  1:1978097/101‑1 (MQ=38)
                                                                                           ccGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGCGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTCcgc       >  2:837622/1‑101 (MQ=38)
                                                                                             gccgcCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAATTGGGGCTGCGCGCCCCCCTCTTGGTCcgctg     <  1:5143820/99‑3 (MQ=255)
                                                                                             gccgcCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTCcgctg     <  2:4050783/99‑3 (MQ=38)
                                                                                             gccgcCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTCcgctg     <  1:409675/99‑3 (MQ=38)
                                                                                             gccgcCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTCcgctg     <  2:2303433/99‑3 (MQ=38)
                                                                                              ccgcCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTCcgctgg    <  2:2115058/100‑4 (MQ=38)
                                                                                               cgcCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTCcgctggc   <  2:4572131/101‑5 (MQ=38)
                                                                                                gcCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTCcgctggcg  >  1:1676320/1‑96 (MQ=38)
                                                                                                       |                                                                                               
GCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGGCCGCGCGGC  >  NC_014034/1607839‑1608037

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG