Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,786,540 Δ1 bp 97.3% coding (674/819 nt) RCAP_rcc01648 → ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,786,5400C→.97.3% 13.5 ‑0.3 ‑1.1259coding (674/819 nt)RCAP_rcc01648ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (120/132):  ref base (4/3):  total (124/135)
Fisher's exact test strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.19e-02

Only 100 of 273 total aligned reads displayed.

AGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CT.GGCCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCT  >  NC_014034/1786441‑1786636
                                                                                                     |                                                                                                
aGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGGc                                                                                                  >  1:1605218/1‑101 (MQ=255)
 gCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGGct                                                                                                 >  2:3006742/1‑100 (MQ=255)
 gCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGGct                                                                                                 <  2:3866591/101‑2 (MQ=255)
  cTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGGctt                                                                                                >  1:5773608/1‑99 (MQ=255)
  cTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGGctt                                                                                                <  1:2413808/101‑3 (MQ=255)
   tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGGCTtt                                                                                               >  1:1342169/1‑101 (MQ=255)
    gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGGCTttc                                                                                              >  1:3743110/1‑100 (MQ=255)
       gCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTa                                                                                          >  1:3435504/1‑101 (MQ=255)
          gCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATcc                                                                                       <  2:1209911/101‑1 (MQ=255)
           cATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCa                                                                                      <  2:1829061/101‑1 (MQ=255)
             tCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATc                                                                                    >  1:902521/1‑101 (MQ=255)
             tCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATc                                                                                    >  2:3169705/1‑101 (MQ=255)
             gcTGAGCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATc                                                                                    <  2:2821604/100‑1 (MQ=255)
              cTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCt                                                                                   >  2:5202527/1‑101 (MQ=255)
              cTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCt                                                                                   >  2:1333962/1‑101 (MQ=255)
              cTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCt                                                                                   <  1:2648876/101‑1 (MQ=255)
                   cgccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAtt                                                                              <  1:5543188/101‑1 (MQ=255)
                   cgccgcGGCGCGGCCGGGGCTTTCGCTGATCTGAAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAtt                                                                              <  1:4975686/101‑1 (MQ=255)
                    gccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAttt                                                                             <  2:852142/101‑1 (MQ=255)
                    gccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAttt                                                                             <  2:3667305/101‑1 (MQ=255)
                         ggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGa                                                                        >  2:2020835/1‑101 (MQ=255)
                         ggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGa                                                                        <  2:3645402/101‑1 (MQ=255)
                         ggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGGGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGa                                                                        <  1:4150953/101‑1 (MQ=255)
                           cgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATg                                                                      <  2:4115968/101‑1 (MQ=255)
                             cgggcgggGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGtt                                                                    >  1:5272449/1‑101 (MQ=255)
                             cgggcgggGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGtt                                                                    <  2:4328037/101‑1 (MQ=255)
                              gggcgtggctttCGCTGATCTGGAAGGGCGTGCTGGTGGTCGAATTT.CTCGGCGGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                   >  2:5448175/10‑101 (MQ=255)
                              gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                   >  2:155236/1‑101 (MQ=255)
                               ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                  <  2:257249/101‑1 (MQ=255)
                               ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                  <  1:2997866/101‑1 (MQ=255)
                               ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                  <  2:3812447/101‑1 (MQ=255)
                                gcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGa                                                                 <  2:5534711/101‑1 (MQ=255)
                                gcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGa                                                                 >  1:2316965/1‑101 (MQ=255)
                                 gggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                <  2:1498136/95‑1 (MQ=255)
                                 cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                <  2:3414763/95‑1 (MQ=255)
                                  ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                               <  1:5469780/96‑1 (MQ=255)
                                   gggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCNTATCCATCTGAATTTCCAGATGTTCGACAt                                                              >  1:784168/5‑101 (MQ=255)
                                     ggTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTg                                                            <  2:1585978/100‑1 (MQ=255)
                                     gctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTg                                                            >  2:1005051/3‑101 (MQ=255)
                                       tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                          >  2:1050916/1‑101 (MQ=255)
                                       tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                          <  2:5244987/101‑1 (MQ=255)
                                       tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                          <  1:4742023/101‑1 (MQ=255)
                                        ttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCg                                                         <  1:4209968/101‑1 (MQ=255)
                                        ttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCg                                                         >  2:619682/1‑101 (MQ=255)
                                        ttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCg                                                         <  1:4267583/101‑1 (MQ=255)
                                          cGCTGATCTGGAAGGTCGTGCTGGTGGTGGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                       <  2:5487461/101‑1 (MQ=255)
                                          cGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                       <  1:4525372/101‑1 (MQ=255)
                                           gCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAgg                                                      >  1:5566394/1‑101 (MQ=255)
                                           gCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAgg                                                      >  2:291341/1‑101 (MQ=255)
                                           gCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAgg                                                      >  2:3199349/1‑101 (MQ=255)
                                            cTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGt                                                     <  2:1115300/101‑1 (MQ=255)
                                            cTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGt                                                     <  1:2954823/101‑1 (MQ=255)
                                             tGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTg                                                    >  1:1879966/1‑101 (MQ=255)
                                                tCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                 <  2:210125/101‑1 (MQ=255)
                                                tCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                 <  1:624804/101‑1 (MQ=255)
                                                tCTGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                 <  1:1005126/101‑1 (MQ=255)
                                                  tGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGc                                               >  1:5054455/1‑101 (MQ=255)
                                                  tGGAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGc                                               >  1:689196/1‑101 (MQ=255)
                                                    gAAGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCt                                             >  1:5387460/1‑101 (MQ=255)
                                                     aaGGTCGTGCTGTTAGTCGGATTTCCG.GGCCGCTCGAGCGGGATAGG.CTTTCGTATCCATCTGAATTGCCAGATGTTCGACATTGCCGAGGTGCTCGCCTg                                            <  2:5041263/101‑1 (MQ=255)
                                                      aGGTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTgg                                           <  1:4682951/101‑1 (MQ=255)
                                                        gTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTAGACATTGCCGAGGTGCTCGCCTGggc                                         >  1:3477811/1‑101 (MQ=255)
                                                        gTCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATC.GTCTTTCGTATCCATCTGAATTTCCAGATTTTCGACATTGCCGATGTGCTCGCCTGggc                                         <  2:5622064/101‑1 (MQ=255)
                                                         tCGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcg                                        <  2:5415933/101‑1 (MQ=255)
                                                          cGTGCTGGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcgg                                       <  1:1991946/101‑1 (MQ=255)
                                                          cGTGCTAGTGGTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcgg                                       <  1:1110385/101‑1 (MQ=255)
                                                               tggtggTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCt                                  >  2:3535098/1‑101 (MQ=255)
                                                               tggtggTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCt                                  >  2:1966435/1‑101 (MQ=255)
                                                               tggtggTCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCt                                  <  1:4637710/101‑1 (MQ=255)
                                                                ggtggtCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCtt                                 >  2:303725/1‑101 (MQ=255)
                                                                 gtggtCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCTGATGTTCGACATTGCCGAGGGGCTCGCCTGGGCGGCGGCttt                                >  2:2260698/1‑101 (MQ=255)
                                                                 gtggtCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCttt                                >  2:1702167/1‑101 (MQ=255)
                                                                  tggtCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTc                               <  1:5200930/101‑1 (MQ=255)
                                                                  tggtCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTc                               <  1:1666651/101‑1 (MQ=255)
                                                                  tggtCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTc                               >  2:4387241/1‑101 (MQ=255)
                                                                  tggtCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTc                               <  1:291374/101‑1 (MQ=255)
                                                                   ggtCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCg                              >  2:5088915/1‑101 (MQ=255)
                                                                    gtCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGt                             <  1:4094044/101‑1 (MQ=255)
                                                                    gtCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGt                             >  2:668631/1‑101 (MQ=255)
                                                                     tCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTc                            <  1:4852118/101‑1 (MQ=255)
                                                                     tCGAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTc                            >  2:4861275/1‑101 (MQ=255)
                                                                       gAATTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTg                          >  1:5368248/1‑101 (MQ=255)
                                                                         aTTT.CTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCg                        <  2:5150660/101‑1 (MQ=255)
                                                                            ttctgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTg                      >  1:2159863/6‑101 (MQ=255)
                                                                            ttctgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTg                      <  1:668688/96‑1 (MQ=255)
                                                                             tctgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGa                     >  2:262614/5‑101 (MQ=255)
                                                                              cggggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGAt                    <  2:3934105/98‑1 (MQ=255)
                                                                                  gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTc                <  1:3963146/101‑1 (MQ=255)
                                                                                   ccGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCg               <  2:1036242/101‑1 (MQ=255)
                                                                                    cGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTctc              >  2:383231/1‑99 (MQ=255)
                                                                                    cGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGc              >  2:5045871/1‑101 (MQ=255)
                                                                                    cGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGc              >  2:4711863/1‑101 (MQ=255)
                                                                                     gCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGcc             <  1:3032046/101‑1 (MQ=255)
                                                                                      cTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCg            <  2:2349068/101‑1 (MQ=255)
                                                                                        cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc          >  1:1529762/1‑101 (MQ=255)
                                                                                         gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         >  1:3210556/1‑101 (MQ=255)
                                                                                            cGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAAt      <  1:5644346/101‑1 (MQ=255)
                                                                                              ggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATgg    <  1:2271998/101‑1 (MQ=255)
                                                                                              ggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATgg    >  2:5461525/1‑101 (MQ=255)
                                                                                                aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCt  >  1:3604721/1‑101 (MQ=255)
                                                                                                     |                                                                                                
AGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTT.CT.GGCCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCT  >  NC_014034/1786441‑1786636

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG