Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,044,270 Δ1 bp 100% intergenic (+143/+34) typA → / ← potG1 GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,044,2700A→.100.0% 73.8 183intergenic (+143/+34)typA/potG1GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG
Reads supporting (aligned to +/- strand):  new base (140/24):  ref base (8/11):  total (148/35)
Fisher's exact test strand distribution p-value = 7.29e-05
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.87e-01

Only 100 of 227 total aligned reads displayed.

CGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAAGGGGGGCGCGAAG.CCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCG  >  NC_014034/2044170‑2044371
                                                                                                    |                                                                                                      
 gAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaa                                                                                                       >  1:4694517/1‑101 (MQ=255)
 gAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaa                                                                                                       >  1:3166754/1‑101 (MQ=255)
  aaTGAAATTCCCCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaa                                                                                                      <  2:857039/101‑1 (MQ=255)
   aTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaa                                                                                                     <  1:4500378/101‑1 (MQ=255)
   aTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaa                                                                                                     <  2:1356888/101‑1 (MQ=255)
   aTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaa                                                                                                     <  1:1952611/101‑1 (MQ=255)
   aTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaa                                                                                                     <  1:2310401/101‑1 (MQ=255)
    tGAAATTCGCCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGCCCGCGGGGGGAATTTGCACAGCaaaaa                                                                                                    <  2:3017681/101‑1 (MQ=255)
    tGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaa                                                                                                    <  2:1302910/101‑1 (MQ=255)
    tGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaa                                                                                                    <  2:3271985/101‑1 (MQ=255)
     gAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaag                                                                                                   <  2:5332780/101‑2 (MQ=255)
     gAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaag                                                                                                   <  2:5416210/101‑2 (MQ=255)
       aaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgg                                                                                                 >  2:4299438/1‑101 (MQ=255)
         ttCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgggg                                                                                               <  1:3936822/101‑1 (MQ=255)
          tCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGggggg                                                                                              >  2:3171196/1‑101 (MQ=255)
           cACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgggggc                                                                                             <  2:4023268/101‑2 (MQ=255)
            aCCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGgcg                                                                                           <  2:4395027/101‑1 (MQ=255)
             ccTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGTAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGgcgc                                                                                          >  1:3627072/1‑101 (MQ=255)
             ccTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGgcgc                                                                                          <  1:5583504/101‑1 (MQ=255)
              cTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGcgcg                                                                                         >  1:4043229/1‑101 (MQ=255)
              cTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGcgcg                                                                                         >  1:1518162/1‑101 (MQ=255)
                ccccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGaa                                                                                       <  1:5299065/101‑1 (MQ=255)
                 cccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAg                                                                                      >  1:5654971/1‑101 (MQ=255)
                    ccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.ccc                                                                                  >  2:4568183/1‑101 (MQ=255)
                    ccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.ccc                                                                                  >  1:3336867/1‑101 (MQ=255)
                    ccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.ccc                                                                                  <  2:2092491/101‑1 (MQ=255)
                     cGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.cccc                                                                                 <  1:2059296/101‑1 (MQ=255)
                      gggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.ccccc                                                                                >  2:3076404/1‑101 (MQ=255)
                      gggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.ccccc                                                                                >  1:343809/1‑101 (MQ=255)
                      gggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.ccccc                                                                                <  1:2457466/101‑1 (MQ=255)
                       ggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.cccccc                                                                               >  2:4103814/1‑101 (MQ=255)
                         atatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCCtt                                                                             <  2:1412894/101‑1 (MQ=255)
                         atatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCCtt                                                                             >  2:4208875/1‑101 (MQ=255)
                           atTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCCtttt                                                                           >  1:1553114/1‑101 (MQ=255)
                           atTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCCtttt                                                                           >  1:2245101/1‑101 (MQ=255)
                             ttGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCCtttttc                                                                         >  2:3065301/1‑100 (MQ=255)
                              tGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCCtttttcc                                                                        >  1:283989/1‑99 (MQ=255)
                              tGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCCtctttcc                                                                        >  2:2315175/1‑99 (MQ=255)
                               gAGGCAATATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCCtttttccg                                                                       >  1:5108150/1‑98 (MQ=255)
                                aGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCCtttttccgt                                                                      >  2:1090919/1‑97 (MQ=255)
                                 ggCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTc                                                                    >  2:3696405/1‑101 (MQ=255)
                                   cAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAg                                                                  >  1:1778160/1‑101 (MQ=255)
                                   cAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAg                                                                  >  1:3056733/1‑101 (MQ=255)
                                   cAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAg                                                                  >  1:122634/1‑101 (MQ=255)
                                   cAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAg                                                                  >  1:1117240/1‑101 (MQ=255)
                                   cAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAg                                                                  >  1:974526/1‑101 (MQ=255)
                                     aGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCg                                                                >  1:786802/1‑101 (MQ=255)
                                        tAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGaaa                                                             >  2:2370530/1‑101 (MQ=255)
                                        tAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGaaa                                                             >  1:4612800/1‑101 (MQ=255)
                                        aaaaGCCTTGCGGGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGaaa                                                             <  2:2596356/100‑1 (MQ=255)
                                         aaaTACTGGCGAGGTTTTCTTCCTTTTCCTGAAAAGCCCGGGGGGGAAATTGGCAAA..AAAAAAGGGGGGCCCCAAC.CCCCCCCTTTTTCCGTCAGCGAAAc                                                            <  1:809184/101‑1 (MQ=255)
                                           aGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAg                                                          >  1:3435123/1‑101 (MQ=255)
                                           aGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAg                                                          >  1:4145911/1‑101 (MQ=255)
                                           aGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAg                                                          >  1:2628000/1‑101 (MQ=255)
                                           aGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAg                                                          >  1:4763026/1‑101 (MQ=255)
                                           aGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAg                                                          >  1:92014/1‑101 (MQ=255)
                                               ttGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAG.AAAAAAGGGGGGCGCGAA..CCCCCCCTTTTTCCGTCAGCGAAACAGCAcc                                                      <  1:2874862/101‑1 (MQ=255)
                                                tGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCa                                                     >  1:3693677/1‑101 (MQ=255)
                                                   ggggTTTTTTTTATCTTTCTGCAAAGACCGGGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCCAAG.CCCCCCTTTTTTCCGTCAGCGAAACAGCACCAgc                                                   <  2:4724461/99‑1 (MQ=255)
                                                   gAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcg                                                  >  1:2135569/1‑101 (MQ=255)
                                                   gAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcg                                                  >  1:3927421/1‑101 (MQ=255)
                                                   gAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcg                                                  >  2:240947/1‑101 (MQ=255)
                                                   gAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcg                                                  >  2:546787/1‑101 (MQ=255)
                                                    aGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcgc                                                 >  1:1425366/1‑101 (MQ=255)
                                                    aGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcgc                                                 >  1:626975/1‑101 (MQ=255)
                                                       tgtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.CAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACAAGCGCGcc                                              >  2:2728535/1‑101 (MQ=255)
                                                        gtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGCAACAGCACCCGCGCGCCg                                             >  1:517404/1‑101 (MQ=255)
                                                        gtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCg                                             >  2:5092083/1‑101 (MQ=255)
                                                          tcttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCggg                                           >  2:3983216/1‑101 (MQ=255)
                                                          tcttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCggg                                           >  1:5500753/1‑101 (MQ=255)
                                                           cttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGc                                          >  2:724864/1‑101 (MQ=255)
                                                             tcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGa                                        >  1:1935143/1‑101 (MQ=255)
                                                              cATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGAc                                       >  1:4288336/1‑101 (MQ=255)
                                                               aTCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGAcc                                      >  2:3821624/1‑101 (MQ=255)
                                                               aTCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGAcc                                      >  2:4890868/1‑101 (MQ=255)
                                                                 cTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAg                                    >  1:1000486/1‑101 (MQ=255)
                                                                 cTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAg                                    >  2:5563347/1‑101 (MQ=255)
                                                                  tttCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAgg                                   >  2:2570685/1‑101 (MQ=255)
                                                                    tCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGcc                                 >  2:499302/1‑101 (MQ=255)
                                                                     cTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCa                                >  2:662937/1‑101 (MQ=255)
                                                                     cTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCa                                >  2:1887795/1‑101 (MQ=255)
                                                                       gCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAcc                              >  1:4980431/1‑101 (MQ=255)
                                                                        cAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGTCGACCAGGCCAccc                             >  1:2352455/1‑101 (MQ=255)
                                                                          aaGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcgc                           >  1:3258858/1‑101 (MQ=255)
                                                                          aaGACCCGGGGGGGAATTTTCACA.AAAAAAAGGGGGGGGCGAAGCCCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAccc                             <  2:4358464/101‑1 (MQ=255)
                                                                            gACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGt                         >  2:2882462/1‑101 (MQ=255)
                                                                             aCCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTc                        >  1:4404481/1‑101 (MQ=255)
                                                                             aCCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTc                        >  2:1052092/1‑101 (MQ=255)
                                                                             aCCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGG.GCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCCCCGGGCGACCAGGCCACCCGCGTCt                       >  1:4914775/1‑101 (MQ=255)
                                                                              ccGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCt                       >  2:4728505/1‑101 (MQ=255)
                                                                                gcgGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTgg                     >  2:1486760/1‑101 (MQ=255)
                                                                                 cgGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTggg                    >  2:3355234/1‑101 (MQ=255)
                                                                                        aattgccacaGC.AAAAAGGGGGGCGGGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGAc             <  2:2675275/95‑1 (MQ=255)
                                                                                         aTTTGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGGCa            >  1:2516715/1‑101 (MQ=255)
                                                                                         aTTTGCACAG.AAAAAGGGGGGGCGCGAA..CCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACa            <  1:3470133/101‑1 (MQ=255)
                                                                                          tttGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACAt           >  1:2393920/1‑101 (MQ=255)
                                                                                          tttGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACAt           >  2:5180662/1‑101 (MQ=255)
                                                                                          tttGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACAt           >  1:1046334/1‑101 (MQ=255)
                                                                                           ttGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATc          >  2:2753259/1‑101 (MQ=255)
                                                                                            tGCACAGC.AAAAAGGGGGGCGCGAAG.CCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATcc         >  1:1071990/1‑101 (MQ=255)
                                                                                                    |                                                                                                      
CGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAAGGGGGGCGCGAAG.CCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCACCG  >  NC_014034/2044170‑2044371

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG