Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,470,253 Δ1 bp 98.2% intergenic (‑186/+31) RCAP_rcc02294 ← / ← proB signal transduction histidine kinase/glutamate 5‑kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,470,2530G→.98.2% 4.8 ‑0.5 ‑0.1223intergenic (‑186/+31)RCAP_rcc02294/proBsignal transduction histidine kinase/glutamate 5‑kinase
Reads supporting (aligned to +/- strand):  new base (97/122):  ref base (1/3):  total (98/125)
Fisher's exact test strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.46e-01

Only 100 of 251 total aligned reads displayed.

CCTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTC  >  NC_014034/2470153‑2470349
                                                                                                    |                                                                                                
ccTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAg                                                                                                  <  2:5532312/101‑1 (MQ=255)
  tGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAACggg                                                                                                <  1:4455791/101‑1 (MQ=255)
   ggCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggg                                                                                               <  1:3756610/101‑1 (MQ=255)
   ggCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGGGTTGATGAAACCAAAgggg                                                                                               >  1:1625642/1‑101 (MQ=255)
   ggCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCAGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggg                                                                                               <  1:2608568/101‑1 (MQ=255)
    gCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAggggg                                                                                              <  1:2789013/101‑1 (MQ=255)
    gCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAggggg                                                                                              <  1:4688241/101‑1 (MQ=255)
     cTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggggg                                                                                             <  1:5281396/101‑1 (MQ=255)
        cGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggggggcc                                                                                          <  1:5554846/101‑3 (MQ=255)
        cGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggggggcc                                                                                          <  1:3580936/101‑3 (MQ=255)
          gcgcGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGGCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGCcct                                                                                        <  1:1707395/101‑2 (MQ=255)
           cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                      <  1:1387094/101‑1 (MQ=255)
            gcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGc                                                                                     >  2:3657214/1‑101 (MQ=255)
             cgaggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcc                                                                                    >  2:2275790/4‑101 (MQ=255)
             cgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcc                                                                                    >  2:3524219/1‑101 (MQ=255)
              ggggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGccc                                                                                   <  1:2956752/101‑1 (MQ=255)
              ggggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGccc                                                                                   <  2:4196129/101‑1 (MQ=255)
              ggggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGccc                                                                                   <  2:2607153/101‑1 (MQ=255)
              ggggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGccc                                                                                   >  1:1748207/1‑101 (MQ=255)
              ggggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcac                                                                                   >  2:1849542/1‑99 (MQ=255)
               gggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAATCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcccc                                                                                  >  2:5730690/1‑101 (MQ=255)
                ggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGccccc                                                                                 <  2:3140511/101‑1 (MQ=255)
                   cGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCctc                                                                              >  1:485703/1‑101 (MQ=255)
                    gTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCctct                                                                             >  1:2316890/1‑101 (MQ=255)
                     ttGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTg                                                                            <  2:4907729/101‑1 (MQ=255)
                     ttGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTg                                                                            <  1:1751869/101‑1 (MQ=255)
                         tgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGcccc                                                                        <  2:2809321/101‑1 (MQ=255)
                          gaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCg                                                                       >  1:4754705/1‑101 (MQ=255)
                          gaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCg                                                                       >  2:3443721/1‑101 (MQ=255)
                               gcgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTc                                                                  <  2:3726536/101‑1 (MQ=255)
                               gcgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTc                                                                  <  1:5730985/101‑1 (MQ=255)
                                cgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCa                                                                 >  1:4019337/1‑101 (MQ=255)
                                 gggCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAg                                                                >  2:1749294/1‑101 (MQ=255)
                                  ggCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGc                                                               >  2:4654618/1‑101 (MQ=255)
                                   gcgcCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGcc                                                              <  2:5021222/101‑1 (MQ=255)
                                    cgcCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCg                                                             <  1:5049004/101‑1 (MQ=255)
                                    cgcCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCg                                                             >  2:1188887/1‑101 (MQ=255)
                                    cgcCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCg                                                             <  1:2346612/101‑1 (MQ=255)
                                     gcCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGa                                                            >  2:1288967/1‑101 (MQ=255)
                                      ccAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGAt                                                           >  1:695324/1‑101 (MQ=255)
                                       cAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATc                                                          >  1:1003595/1‑101 (MQ=255)
                                       cAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATc                                                          >  1:1772497/1‑101 (MQ=255)
                                         aaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCAc                                                        >  2:5143780/1‑101 (MQ=255)
                                          aGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCAcc                                                       >  1:3943350/1‑101 (MQ=255)
                                          aGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCCCTGCCCCGCCCTCAGCCGATCAcc                                                       >  1:2924889/1‑101 (MQ=255)
                                            gAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCAt                                                     <  1:4000441/101‑1 (MQ=255)
                                            gAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCAt                                                     >  2:5271601/1‑101 (MQ=255)
                                            gAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCAt                                                     >  1:5120113/1‑101 (MQ=255)
                                            gAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCAt                                                     <  1:4266957/101‑1 (MQ=255)
                                              aCGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATgt                                                   <  1:590635/101‑1 (MQ=255)
                                                  gcCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATgtcgtc                                               <  1:3095943/101‑1 (MQ=255)
                                                  gcCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATgtcgtc                                               <  2:2112429/101‑1 (MQ=255)
                                                    ccGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTcgc                                             <  1:3298693/101‑1 (MQ=255)
                                                    ccGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTcgc                                             <  1:4300696/101‑1 (MQ=255)
                                                       gATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGAt                                          >  2:5741747/1‑101 (MQ=255)
                                                        aTCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATg                                         <  1:3902519/101‑1 (MQ=255)
                                                          cAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGca                                       <  2:4021817/101‑1 (MQ=255)
                                                          cAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGca                                       <  1:301145/101‑1 (MQ=255)
                                                          cAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGca                                       <  1:849184/101‑1 (MQ=255)
                                                          cAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGca                                       <  2:1151350/101‑1 (MQ=255)
                                                            aCGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcaca                                     <  1:5162457/101‑1 (MQ=255)
                                                              gccgccCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAg                                   <  2:4477826/101‑1 (MQ=255)
                                                              gccgccCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAg                                   <  1:2152041/101‑1 (MQ=255)
                                                              gccgccCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAg                                   >  2:2768480/1‑101 (MQ=255)
                                                              gccgccCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAg                                   <  1:1859161/101‑1 (MQ=255)
                                                               ccgcccgcGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgc                                  <  1:3524436/101‑1 (MQ=255)
                                                                  cccgcGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgcg                               <  2:3002171/101‑1 (MQ=255)
                                                                  cccgcGAGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgcg                               <  1:2661665/101‑1 (MQ=255)
                                                                   ccgcGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgcgg                              >  1:3767119/1‑101 (MQ=255)
                                                                    cgcgcgCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgcggc                             >  1:950381/1‑101 (MQ=255)
                                                                    cgcgcgCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCACTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgcggc                             <  1:4718697/101‑1 (MQ=255)
                                                                     gcgcgcAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgcggcg                            <  2:1697440/101‑1 (MQ=255)
                                                                         gcAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCgcggcgcggc                        >  2:2396911/1‑101 (MQ=255)
                                                                         gcAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCgcggcgcggc                        <  1:36827/101‑1 (MQ=255)
                                                                          cAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGcc                       <  1:2007787/101‑1 (MQ=255)
                                                                          cAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGcc                       >  1:130719/1‑101 (MQ=255)
                                                                           aaaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGccc                      >  1:252326/1‑101 (MQ=255)
                                                                            aaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCg                     >  1:1119606/1‑101 (MQ=255)
                                                                            aaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCg                     >  2:4735409/1‑101 (MQ=255)
                                                                             aCCCGGGTGTTGATGAAACCAAA.GGGGCGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCgg                    <  2:3036780/101‑1 (MQ=255)
                                                                                cGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATa                 <  1:3417866/101‑1 (MQ=255)
                                                                                cGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATa                 <  1:2887596/101‑1 (MQ=255)
                                                                                 gggTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCTCCGGCCTCAGCCGATCACCATTTCGTCCTGGTGCACTTGCGCGCTGCGGCCCGGATAg                >  2:1685647/1‑101 (MQ=255)
                                                                                  ggTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGc               <  1:2628443/101‑1 (MQ=255)
                                                                                   gtgtTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGcc              >  2:1460809/1‑101 (MQ=255)
                                                                                    tgtTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGccc             >  1:2676167/1‑101 (MQ=255)
                                                                                      ttgattgatccAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcag           <  2:441783/92‑1 (MQ=255)
                                                                                      ttGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcag           <  2:695267/101‑1 (MQ=255)
                                                                                      ttGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcag           >  1:2558113/1‑101 (MQ=255)
                                                                                      ttGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcag           <  2:3302078/101‑1 (MQ=255)
                                                                                       tgatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagc          <  2:2038771/101‑1 (MQ=255)
                                                                                        gatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagca         <  2:3916599/101‑1 (MQ=255)
                                                                                         atgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagcag        >  1:2775440/1‑101 (MQ=255)
                                                                                         atgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagcag        <  2:1932495/101‑1 (MQ=255)
                                                                                            aaaCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAgcgc     <  1:4247131/101‑1 (MQ=255)
                                                                                             aaCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGcc    >  2:5329151/1‑101 (MQ=255)
                                                                                             aaCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAACCGCGGCGCGGCCCGCATAGCCCAGCAGCGcc    >  1:1913918/1‑101 (MQ=255)
                                                                                              aCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCCGCGccc   >  1:1794615/1‑100 (MQ=255)
                                                                                              aCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCt   <  2:4331570/101‑1 (MQ=255)
                                                                                               ccAAA.GGGGGGGCCCTCCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTc  <  2:1622273/101‑1 (MQ=255)
                                                                                                    |                                                                                                
CCTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTC  >  NC_014034/2470153‑2470349

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG