Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,551,310 Δ1 bp 100% intergenic (‑45/+124) RCAP_rcc02379 ← / ← ftsI CobW/HypB/UreG family cobalamin biosynthesis protein/peptidoglycan synthetase FtsI

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,551,3100A→.100.0% 1002.5 227intergenic (‑45/+124)RCAP_rcc02379/ftsICobW/HypB/UreG family cobalamin biosynthesis protein/peptidoglycan synthetase FtsI
Reads supporting (aligned to +/- strand):  new base (123/103):  ref base (0/0):  total (124/103)

Only 100 of 259 total aligned reads displayed.

AGAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGAAAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGCCGG  >  NC_014034/2551210‑2551406
                                                                                                    |                                                                                                 
aGAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGa                                                                                                   >  1:248017/1‑101 (MQ=255)
 gAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaa                                                                                                  >  1:4312829/1‑101 (MQ=255)
 gAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaa                                                                                                  >  1:2198788/1‑101 (MQ=255)
 gAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaa                                                                                                  <  2:475923/101‑1 (MQ=255)
 gAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaa                                                                                                  <  2:4613663/101‑1 (MQ=255)
  aaaGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaag                                                                                                 >  2:162312/1‑100 (MQ=255)
  aaaGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaag                                                                                                 >  2:5188614/1‑100 (MQ=255)
  aaaGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaag                                                                                                 <  2:4856247/101‑2 (MQ=255)
   aaGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaagc                                                                                                <  1:673803/101‑3 (MQ=255)
    aGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaagcc                                                                                               <  1:4984075/101‑4 (MQ=255)
    aGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaagcc                                                                                               <  1:3193767/101‑4 (MQ=255)
    aGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGaagcc                                                                                               >  2:1737054/1‑98 (MQ=255)
         gAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAgcccgccc                                                                                         >  1:2937704/1‑101 (MQ=255)
          aaaGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGcccc                                                                                        >  1:764467/1‑101 (MQ=255)
          aaaGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGcccc                                                                                        >  1:2389256/1‑101 (MQ=255)
           aaGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGccccc                                                                                       <  1:488433/101‑1 (MQ=255)
            aGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGcccccc                                                                                      <  2:885755/101‑1 (MQ=255)
             gCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGccccccc                                                                                     <  1:3411196/101‑1 (MQ=255)
                 gCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCaa                                                                                 <  1:540974/101‑1 (MQ=255)
                    gACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGcc                                                                              >  2:2093390/1‑101 (MQ=255)
                     aCCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGccc                                                                             <  2:3308623/101‑1 (MQ=255)
                       cGGAAGGCGGGGATCAGTGGGAGAAAGGGCCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGc                                                                           <  2:5001144/101‑1 (MQ=255)
                        ggAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGcc                                                                          >  2:4465217/1‑101 (MQ=255)
                        ggAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGcc                                                                          >  2:2841596/1‑101 (MQ=255)
                          aaGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCcaa                                                                        >  1:3168818/1‑101 (MQ=255)
                               ggggATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCcaaagcaa                                                                   >  2:2138612/1‑101 (MQ=255)
                               ggggATCAGTGGGAGAAAGGGTCATGGACGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGACCGCCCCCCCGCAAGCCCGCcaaagcaa                                                                   >  2:1490681/1‑101 (MQ=255)
                                 ggATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCcaaagcaaag                                                                 <  1:2174660/101‑1 (MQ=255)
                                  gATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAgg                                                                <  1:5065585/101‑1 (MQ=255)
                                   aTCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTACCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAggg                                                               >  2:4379637/1‑101 (MQ=255)
                                      aGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCg                                                            >  1:5096381/1‑101 (MQ=255)
                                      aGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCACCCCGCAAGCCCGCCAAAGCAAAGTGCCg                                                            >  2:2651515/1‑101 (MQ=255)
                                       gTGGGAGAAAGGGTCATGGTCGTCCATCTGTGTTATGTTATACAATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCgg                                                           <  1:362008/101‑1 (MQ=255)
                                       gTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCgg                                                           <  2:1710072/101‑1 (MQ=255)
                                       gTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCgg                                                           <  1:1363603/101‑1 (MQ=255)
                                       gTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCgg                                                           >  1:5778273/1‑101 (MQ=255)
                                       gTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCgg                                                           >  2:1381624/1‑101 (MQ=255)
                                        tGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGa                                                          >  1:4273472/1‑101 (MQ=255)
                                        tGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGa                                                          >  1:76749/1‑101 (MQ=255)
                                         gggAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGAc                                                         <  1:5196741/101‑1 (MQ=255)
                                          ggAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGAcc                                                        <  2:5761597/101‑1 (MQ=255)
                                           gagaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGAccc                                                       >  1:2773153/1‑101 (MQ=255)
                                            agaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCt                                                      <  2:2389122/101‑1 (MQ=255)
                                            agaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCt                                                      >  2:2807184/1‑101 (MQ=255)
                                            agaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCt                                                      <  1:3059816/101‑1 (MQ=255)
                                            agaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCt                                                      <  2:1567620/101‑1 (MQ=255)
                                            agaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCt                                                      >  2:5269527/1‑101 (MQ=255)
                                             gaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTg                                                     <  2:1150964/101‑1 (MQ=255)
                                             gaAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTg                                                     >  2:4088729/1‑101 (MQ=255)
                                              aaaGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGc                                                    >  2:3115097/1‑101 (MQ=255)
                                              aaaGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGc                                                    <  2:3184228/101‑1 (MQ=255)
                                              aaaGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGc                                                    >  1:813161/1‑101 (MQ=255)
                                               aaGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCg                                                   >  2:2552758/1‑101 (MQ=255)
                                                aGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCgg                                                  <  1:1577988/101‑1 (MQ=255)
                                                  ggTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGcc                                                >  2:1171685/1‑101 (MQ=255)
                                                  ggTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGcc                                                >  1:5668835/1‑101 (MQ=255)
                                                   gTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGccc                                               >  1:2336139/1‑101 (MQ=255)
                                                    tCATGGCCGTCCATCTGAGTTATGTTATACTATCCCCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCg                                              <  2:5603366/101‑1 (MQ=255)
                                                    tCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCg                                              <  2:3285760/101‑1 (MQ=255)
                                                     cATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGa                                             >  2:4051666/1‑101 (MQ=255)
                                                     cATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGa                                             >  1:3382929/1‑101 (MQ=255)
                                                        ggCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.ccc                                         <  2:3495985/101‑1 (MQ=255)
                                                         gCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.cccc                                        <  1:69113/101‑1 (MQ=255)
                                                            gcccATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.ccccgtg                                     <  2:1960235/99‑1 (MQ=255)
                                                            gTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.ccccgtg                                     >  1:3257370/1‑101 (MQ=255)
                                                              ccATCTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.ccccgtgtt                                   <  1:3501046/101‑1 (MQ=255)
                                                                  cTGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCC.AAGCAAAGGGCCGGACCCTGCGGCCCCACccccgggttttcc                               >  1:5503211/1‑101 (MQ=255)
                                                                   tGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.ccccgtgttttccc                              >  2:5237036/1‑101 (MQ=255)
                                                                   tGAGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.ccccgcgttttccc                              >  2:2419442/1‑101 (MQ=255)
                                                                     aGTTATGTTATACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.ccccgtgttttccccg                            >  1:3976538/1‑101 (MQ=255)
                                                                       ttatgttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCACCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.ccccgtgttttccccgtg                          >  1:47551/1‑101 (MQ=255)
                                                                         atgttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.ccccgtgttttccccgtgtt                        >  2:3766495/1‑101 (MQ=255)
                                                                          tgttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.ccccgtgttttccccgtgttt                       <  1:162332/101‑1 (MQ=255)
                                                                          tgttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.ccccgtgttttccccgtgttt                       >  2:642133/1‑101 (MQ=255)
                                                                           gttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.ccccgtgttttccccgtgtttt                      >  1:5508259/1‑101 (MQ=255)
                                                                           gttatACTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.ccccgtgttttccccgtgtttt                      <  1:4558706/101‑1 (MQ=255)
                                                                              ataCTATCACCCAAATAG.CCGGAGGCCCGCCCCCCTGCAGGCCCGCCAAAGCAAAGGGCCGGTCCCTGCGGCCCGC.CCCCGTGTTTTCCCCGTGTTTTTcc                   <  2:1817197/101‑1 (MQ=255)
                                                                               taCTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCt                  <  2:5687573/101‑1 (MQ=255)
                                                                                aCTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTg                 <  2:4311321/101‑1 (MQ=255)
                                                                                aCTATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTg                 >  2:2274952/1‑101 (MQ=255)
                                                                                  tATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGt               >  2:5535062/1‑101 (MQ=255)
                                                                                  tATCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGt               >  1:5591861/1‑101 (MQ=255)
                                                                                   aTCACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTg              <  1:4432893/101‑1 (MQ=255)
                                                                                     cACCCAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCa            >  1:1225649/1‑101 (MQ=255)
                                                                                      aCCCAAATAG.CCGGAAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCaa           >  1:3130798/1‑101 (MQ=255)
                                                                                        cgaaaTAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAAt         <  1:1339595/99‑1 (MQ=255)
                                                                                        ccAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAAt         >  1:5307768/1‑101 (MQ=255)
                                                                                        ccAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAAt         >  1:4142550/1‑101 (MQ=255)
                                                                                         cAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATa        <  2:666341/101‑1 (MQ=255)
                                                                                         cAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATa        <  1:4379904/101‑1 (MQ=255)
                                                                                         cAAATAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATa        >  2:3510106/1‑101 (MQ=255)
                                                                                          aaaTAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATAc       <  1:1171766/101‑1 (MQ=255)
                                                                                           aaTAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACg      >  1:3888785/1‑101 (MQ=255)
                                                                                            aTAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATAcag     >  2:4295689/1‑99 (MQ=255)
                                                                                            aTAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGc     >  1:1882664/1‑101 (MQ=255)
                                                                                             tAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGcc    >  2:5526352/1‑101 (MQ=255)
                                                                                             tAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGcc    <  1:2635069/101‑1 (MQ=255)
                                                                                             tAGCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGcc    >  2:2290464/1‑101 (MQ=255)
                                                                                               gCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGCCgg  <  2:5370769/101‑1 (MQ=255)
                                                                                               gCCCG.AAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGCCgg  >  1:2890234/1‑101 (MQ=255)
                                                                                                    |                                                                                                 
AGAAAGCCCGAAAGCACGCTGACCGGAAGGCGGGGATCAGTGGGAGAAAGGGTCATGGCCGTCCATCTGAGTTATGTTATACTATCACCCAAATAGCCCGAAAGCCCGCCCCCCCGCAAGCCCGCCAAAGCAAAGGGCCGGACCCTGCGGCCCGA.CCCCGTGTTTTCCCCGTGTTTTTCCTGGTGCAAATACGCCGG  >  NC_014034/2551210‑2551406

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG