Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,617,778 Δ1 bp 100% intergenic (‑152/‑462) RCAP_rcc02439 ← / → RCAP_rcc02440 hypothetical protein/AsnC/Lrp family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,617,7780C→.100.0% 20.4 208intergenic (‑152/‑462)RCAP_rcc02439/RCAP_rcc02440hypothetical protein/AsnC/Lrp family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (113/85):  ref base (3/6):  total (116/92)
Fisher's exact test strand distribution p-value = 1.85e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.64e-05

Only 100 of 261 total aligned reads displayed.

GCGGGGATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTCCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAGA  >  NC_014034/2617678‑2617878
                                                                                                    |                                                                                                    
gcggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTc                                                                                                      <  2:5240600/101‑1 (MQ=255)
 cggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcc                                                                                                     >  2:5478594/1‑101 (MQ=255)
 cggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcc                                                                                                     <  1:4724219/101‑1 (MQ=255)
    ggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccc                                                                                                  >  1:3381496/1‑101 (MQ=255)
    ggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccc                                                                                                  >  1:3435779/1‑101 (MQ=255)
      aTCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccc                                                                                                <  2:2911445/101‑1 (MQ=255)
       tCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccccc                                                                                               <  2:5311943/101‑1 (MQ=255)
        cAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccg                                                                                              >  1:1002206/1‑100 (MQ=255)
        cAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccg                                                                                              >  1:5496036/1‑100 (MQ=255)
        cAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCCGCTccccccccg                                                                                              <  1:3143150/101‑2 (MQ=255)
        cAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTCGACAGCGACAGCGGCCTCCCGGCTccccccccg                                                                                              >  1:4971954/1‑100 (MQ=255)
           ggCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccggcc                                                                                           >  2:237060/1‑97 (MQ=255)
             cggaaaTCCGGGCCGCCGCCACAGCCCAGCTGCCGGCCGCCGACGAGGTCGAAATGCAGCGGGGACAGGGCCAGCGGCCCCCCGGC.CCCCCCCCCGGCCaa                                                                                        <  2:3381291/97‑1 (MQ=255)
             ccGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaa                                                                                        <  1:1024231/101‑1 (MQ=255)
              cGGAATTCGGGCATCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaaa                                                                                       <  2:615935/101‑1 (MQ=255)
               ggAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAg                                                                                      >  2:1985307/1‑101 (MQ=255)
                gAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGc                                                                                     >  2:2214182/1‑101 (MQ=255)
                  atttcggCAGCCGAAACAGCGCAGCCTACAGGCGCCCACGATGGCGAAAAGCAGGGGGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGccc                                                                                   <  2:2569079/96‑1 (MQ=255)
                  aTTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGccc                                                                                   >  1:1142309/1‑101 (MQ=255)
                   ttCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGcccc                                                                                  <  1:1118111/101‑1 (MQ=255)
                    tCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCg                                                                                 <  1:540679/101‑1 (MQ=255)
                       ggCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGcc                                                                              <  2:3435574/101‑1 (MQ=255)
                          aGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGa                                                                           >  2:3149855/1‑101 (MQ=255)
                             cGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTc                                                                        >  1:3829635/1‑101 (MQ=255)
                              gACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT..CCCCCCCGGCCAAAGCCCCGGCCCGACTCGt                                                                      >  2:5386106/1‑101 (MQ=255)
                               acacAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGccccccggga                                                                      >  2:3584363/1‑94 (MQ=255)
                               acacAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGt                                                                      <  1:5286993/101‑1 (MQ=255)
                                cacaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTc                                                                     >  1:3171089/1‑101 (MQ=255)
                                cacaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTc                                                                     >  1:5268811/1‑101 (MQ=255)
                                  caGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCNCCGGCCCGACTCGTCTg                                                                   >  2:428662/1‑101 (MQ=255)
                                  aagcggtgCTGACAGCCGCTGTCGATGTCGAAATGCAGGGTGGACAGCGACAGCGGCCCCCCGGCT.CCCCCCCCGGCCAAAGCCCCGTCCCGACTCGTCTg                                                                   <  2:4765298/94‑1 (MQ=255)
                                   aGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGGCAAAGCCCCGGCCCGACTCGTCTGt                                                                  <  2:4580227/101‑1 (MQ=255)
                                   aGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGt                                                                  >  2:2546632/1‑101 (MQ=255)
                                    gcgcAGCTCACAGCCGCCGACGATGTCAAAATGCAGCGTGGACAGCGACCGCGGCCCCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTc                                                                 <  2:4844791/101‑1 (MQ=255)
                                     cgcAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTggcc                                                                >  1:132829/1‑98 (MQ=255)
                                     cgcAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTcg                                                                <  2:2066247/101‑1 (MQ=255)
                                        aGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCgcgg                                                             <  1:1592461/101‑1 (MQ=255)
                                         gCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCgcggc                                                            <  1:2214304/101‑1 (MQ=255)
                                           tGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCACCGGCCCGACTCGTCTGTCGCGGCGa                                                          >  1:4860599/1‑101 (MQ=255)
                                            gACAGCCGCCGGCGATTTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTTGCGGCGAc                                                         <  2:1146945/101‑1 (MQ=255)
                                            gACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGGGAc                                                         >  1:1897312/1‑101 (MQ=255)
                                            gACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGAc                                                         <  2:223573/101‑1 (MQ=255)
                                            gACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGAc                                                         >  2:3790609/1‑101 (MQ=255)
                                            gACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGAc                                                         <  2:3136950/101‑1 (MQ=255)
                                             aCAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACg                                                        >  1:562035/1‑101 (MQ=255)
                                                gccgccGACGAGGTCGAAATGCAGCGTGGACGGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAt                                                     <  1:2688078/101‑1 (MQ=255)
                                                   gccgACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAtgtg                                                  <  1:2458109/101‑1 (MQ=255)
                                                   cccgACGATGTCGAAACGCAGCGTGGACAGCGACAGCGCCCTCCCGGC.CCCCCCCCCGGCCAAACCCCCGGCCCGCCTCGTCTGTCGCGGCGACGGAtgtg                                                  <  2:3396640/100‑1 (MQ=255)
                                                    ccgACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGGCCGACTCGACTGTCGCGGCGCCGGATgggt                                                 >  2:2273590/1‑101 (MQ=255)
                                                     cgacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTc                                                >  2:3725472/1‑101 (MQ=255)
                                                     cgacgaGGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTc                                                <  1:4238973/101‑1 (MQ=255)
                                                      gacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCa                                               >  2:2678386/1‑101 (MQ=255)
                                                       acgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCAt                                              >  2:1513978/1‑101 (MQ=255)
                                                       acgaTGTCGAAAGGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCAt                                              <  2:1386615/101‑1 (MQ=255)
                                                        cgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATg                                             <  1:5478884/101‑1 (MQ=255)
                                                         gaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATgg                                            >  1:3244558/1‑101 (MQ=255)
                                                         gaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATgg                                            >  1:4467836/1‑101 (MQ=255)
                                                          aTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATggg                                           <  2:4650898/101‑1 (MQ=255)
                                                           tGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGc                                          >  1:4440838/1‑101 (MQ=255)
                                                            gTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCt                                         >  1:3720802/1‑101 (MQ=255)
                                                              cGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCttt                                       <  1:3919896/101‑1 (MQ=255)
                                                               gAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCACGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTc                                      <  1:4389125/101‑1 (MQ=255)
                                                                 aaTGCAGCGTGGACAGCGAGAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGc                                    >  2:73784/1‑101 (MQ=255)
                                                                  aTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCa                                   >  1:3659540/1‑101 (MQ=255)
                                                                  aTGCAGCGGGGACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCa                                   <  1:3005135/101‑1 (MQ=255)
                                                                     cAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGcc                                >  1:2096231/1‑101 (MQ=255)
                                                                       gCGTGCACAGCGACAGCGGCCCCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTc                              <  2:4022763/101‑1 (MQ=255)
                                                                         gTGGCCAGCGGCAGCGGCCGCCCGGCT.CCCCCCCCGGCAGAACCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTccc                            <  1:1436065/101‑1 (MQ=255)
                                                                          tGGCCAGCGCCAGCGGCCCCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCg                           <  1:2238691/101‑1 (MQ=255)
                                                                          tGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCg                           <  2:2739977/101‑1 (MQ=255)
                                                                          tGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCg                           <  2:740244/101‑1 (MQ=255)
                                                                          tGGACAGCGACAGCGCCCCCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCg                           <  1:2150808/101‑1 (MQ=255)
                                                                            gccagcgccagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGCCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGaa                         <  1:4037538/99‑1 (MQ=255)
                                                                            gacagcgacagcGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGaa                         <  1:3621997/101‑1 (MQ=255)
                                                                            gacagcgacagcGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGaa                         <  2:5163075/101‑1 (MQ=255)
                                                                             acacggcaaccgGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAg                        <  2:1785507/91‑1 (MQ=255)
                                                                               agcgacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGtt                      <  1:2042088/101‑1 (MQ=255)
                                                                                gcgacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTg                     <  1:173096/101‑1 (MQ=255)
                                                                                 cgacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgc                    <  2:5725241/101‑1 (MQ=255)
                                                                                  gacagcgGCCTCCCGGCT.CCCGCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAGGTGTCATGGGCTTTCGCCGCCCCCCCCAGTTgcg                   >  1:3273269/1‑101 (MQ=255)
                                                                                  gacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcg                   >  1:2664954/1‑101 (MQ=255)
                                                                                   acagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcgc                  >  2:1825679/1‑101 (MQ=255)
                                                                                   acagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcgc                  <  2:3244358/101‑1 (MQ=255)
                                                                                   acagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcgc                  <  1:2519752/101‑1 (MQ=255)
                                                                                   acagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCCCCCGAAGTTgcgc                  >  1:1575563/1‑101 (MQ=255)
                                                                                    cagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCa                 >  1:5751810/1‑101 (MQ=255)
                                                                                     agcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCaa                >  2:129357/1‑101 (MQ=255)
                                                                                     agcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCAGatcggaagagc                >  2:4226686/1‑91 (MQ=255)
                                                                                      gcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGGGACGGATGGGTAATGGGCTTTCGCAGCCTCCCGAAGTTGGGCAAc               >  1:10655/1‑101 (MQ=255)
                                                                                      gcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAc               >  1:992367/1‑101 (MQ=255)
                                                                                       cgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAcc              >  2:5528578/1‑101 (MQ=255)
                                                                                        ggCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAccc             <  2:577879/101‑1 (MQ=255)
                                                                                           cTCCCGGCT.CCCCCCCCGGCCAAAGCCACGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCcgcg          <  2:511602/101‑1 (MQ=255)
                                                                                             cccGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGc        >  2:4969175/1‑101 (MQ=255)
                                                                                             cccGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGc        <  1:4270457/101‑1 (MQ=255)
                                                                                              ccGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCt       <  1:1914467/101‑1 (MQ=255)
                                                                                               ccggccccccccccGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCt       <  1:751566/96‑1 (MQ=255)
                                                                                               cGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTg      <  1:237093/101‑1 (MQ=255)
                                                                                               cGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTg      <  2:952604/101‑1 (MQ=255)
                                                                                                    cccccccccGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAGa  <  2:4418062/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GCGGGGATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTCCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAGA  >  NC_014034/2617678‑2617878

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG