Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,201 Δ1 bp 81.7% intergenic (‑177/+99) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2010A→.81.7% 130.2 ‑1.2 ‑1.4169intergenic (‑177/+99)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (80/58):  ref base (14/16):  total (95/74)
Fisher's exact test strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.63e-02

Only 100 of 183 total aligned reads displayed.

AGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640102‑2640297
                                                                                                     |                                                                                                
 ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                                 <  2:2036113/101‑1 (MQ=25)
    gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                              <  1:154107/101‑3 (MQ=25)
         aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                        <  1:4438297/101‑1 (MQ=21)
         aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                        <  1:4009758/101‑1 (MQ=25)
           ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                      <  2:4538823/101‑1 (MQ=25)
           ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                      <  2:3086863/101‑1 (MQ=25)
              tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                   <  2:4230573/101‑1 (MQ=25)
               ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                                                                  <  2:535389/101‑1 (MQ=25)
                  gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCt                                                                               <  1:1050008/101‑1 (MQ=25)
                       tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCt                                                                          <  2:2800121/101‑1 (MQ=25)
                        gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                         <  2:4110343/101‑1 (MQ=25)
                        gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                         <  2:4815659/101‑1 (MQ=25)
                             ggCAAAAGGGGGGCGCTGCCCCCCTCTTTGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.GAAGACCATGGGTTCATTTTGCCTCAAata                                                                    >  2:4396765/1‑101 (MQ=21)
                              gCAAAAGGGGCGCGCCTCCCCCCTC.TTGGCCTTCGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAatat                                                                   <  2:2183357/101‑1 (MQ=25)
                               tgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCTGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                                 <  1:4925300/99‑1 (MQ=17)
                                gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                <  1:3834882/100‑1 (MQ=17)
                                gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCCGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCCAATATccc                                                                >  1:2075324/2‑101 (MQ=9)
                                 aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               >  2:4791669/1‑101 (MQ=17)
                                 aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               <  1:4119471/101‑1 (MQ=17)
                                 aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               <  1:5639432/101‑1 (MQ=17)
                                 aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               <  2:24664/101‑1 (MQ=17)
                                  aaagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                              >  1:1855996/4‑101 (MQ=17)
                                  aaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                               <  2:4554574/101‑1 (MQ=25)
                                   aagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                             <  1:2114397/99‑1 (MQ=18)
                                   aagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                             >  1:1167496/3‑101 (MQ=18)
                                   aagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                             >  1:1867500/3‑101 (MQ=18)
                                   aagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGACCCAAATATCCCggg                                                             >  1:3353817/3‑101 (MQ=17)
                                    ggggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                             >  1:5782600/1‑101 (MQ=21)
                                    agggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                            <  2:5208637/100‑1 (MQ=18)
                                     gggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                           <  1:2358007/101‑1 (MQ=18)
                                     gggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                            >  2:4815310/1‑101 (MQ=25)
                                       gggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                         <  1:3393296/101‑3 (MQ=18)
                                       gggCCCCGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                          <  1:3942700/101‑1 (MQ=21)
                                        ggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                         <  2:1184804/101‑1 (MQ=25)
                                           gcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                      >  1:3601608/1‑101 (MQ=25)
                                           gcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                      >  2:2350610/1‑101 (MQ=25)
                                            cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                                    >  2:3327273/1‑94 (MQ=25)
                                             tGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggggaggcga                                                   >  1:1368990/1‑94 (MQ=21)
                                             tGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                    >  2:684030/1‑101 (MQ=25)
                                               ccccccTC.TTGGCCTTTGGCCAATTCACCCCCCGAGGTATTTGGAGCCAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCtta                                                  >  1:1424258/1‑99 (MQ=25)
                                                cccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGGCCTGaa                                                 >  2:3938958/1‑101 (MQ=25)
                                                 ccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                >  2:597142/1‑101 (MQ=37)
                                                  cccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                               >  2:4566733/1‑101 (MQ=37)
                                                    ctc.tTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                             <  1:5235355/101‑1 (MQ=37)
                                                    ctc.tTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                             >  2:4410668/1‑101 (MQ=37)
                                                      c.tTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                           <  2:2122091/101‑1 (MQ=37)
                                                        ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                          <  1:1039159/96‑1 (MQ=37)
                                                         tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                         >  1:1488014/5‑101 (MQ=37)
                                                         tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                         >  2:85464/5‑101 (MQ=37)
                                                         tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                         >  1:1281741/5‑101 (MQ=37)
                                                           gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                       >  2:1477467/3‑101 (MQ=37)
                                                            ccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                      <  2:5782305/100‑1 (MQ=37)
                                                             cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                     >  2:1790986/1‑101 (MQ=37)
                                                               tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                   >  2:112307/1‑101 (MQ=255)
                                                                cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGTGAGTCCTGAAAGGACGGGGGGCaga                                  >  1:3523901/1‑101 (MQ=37)
                                                                cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                  >  1:83945/1‑101 (MQ=255)
                                                                   ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                               >  1:3694064/1‑101 (MQ=37)
                                                                    cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                              >  1:29023/1‑101 (MQ=37)
                                                                    cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                              >  1:3734828/1‑101 (MQ=37)
                                                                    cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                              >  1:617288/1‑101 (MQ=255)
                                                                     aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                             >  1:4386708/1‑101 (MQ=37)
                                                                      aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAATGACGGGGGGCAGAGccccc                            >  1:3248765/1‑101 (MQ=37)
                                                                      aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                            >  2:3229352/1‑101 (MQ=37)
                                                                       ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                           >  2:1132129/1‑101 (MQ=25)
                                                                       ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                           >  1:4694849/1‑101 (MQ=25)
                                                                       ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                           >  1:4570900/1‑101 (MQ=25)
                                                                        tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                          >  1:2473088/4‑101 (MQ=255)
                                                                          accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                        >  2:5034044/2‑101 (MQ=255)
                                                                          accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                        >  2:2696365/2‑101 (MQ=255)
                                                                          accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                        >  2:5580576/2‑101 (MQ=255)
                                                                           ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                       <  1:176/101‑1 (MQ=255)
                                                                            cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                      <  2:952536/101‑1 (MQ=255)
                                                                             ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                     >  2:3234337/1‑101 (MQ=255)
                                                                               ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCCCCCgggc                   >  1:2370244/1‑100 (MQ=25)
                                                                                cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                  >  2:1638340/1‑101 (MQ=255)
                                                                                 gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                 <  1:1950310/101‑1 (MQ=255)
                                                                                 gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                 >  2:1697365/1‑101 (MQ=255)
                                                                                  aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                >  1:160650/1‑101 (MQ=255)
                                                                                    gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc              >  1:1453986/1‑101 (MQ=255)
                                                                                    gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc              >  2:4066177/1‑101 (MQ=255)
                                                                                       tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           <  1:4713131/101‑1 (MQ=255)
                                                                                       tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           <  1:4170348/101‑1 (MQ=255)
                                                                                       tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           <  2:3717429/101‑1 (MQ=255)
                                                                                        ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa          >  1:4063216/1‑101 (MQ=255)
                                                                                         tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg         >  1:5784336/1‑101 (MQ=255)
                                                                                          ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        >  1:2249088/1‑101 (MQ=255)
                                                                                          ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        >  1:4494020/1‑101 (MQ=255)
                                                                                           gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg       <  2:881500/98‑1 (MQ=255)
                                                                                           gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg       >  2:3889789/4‑101 (MQ=255)
                                                                                           gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg       >  2:3360556/4‑101 (MQ=255)
                                                                                              cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc    >  2:3863652/1‑101 (MQ=255)
                                                                                               aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCgagat   >  2:3414713/1‑97 (MQ=255)
                                                                                               aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc   >  1:3851618/1‑101 (MQ=255)
                                                                                               aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc   >  1:4236108/1‑101 (MQ=255)
                                                                                               aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc   >  2:3592181/1‑101 (MQ=255)
                                                                                               aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc   >  1:5689722/1‑101 (MQ=255)
                                                                                               aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCTTTCTCAAGCGTTcc   >  2:1409480/1‑101 (MQ=255)
                                                                                                aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  <  1:2994544/101‑1 (MQ=255)
                                                                                                aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  >  2:315776/1‑101 (MQ=255)
                                                                                                aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  <  1:910326/101‑1 (MQ=255)
                                                                                                     |                                                                                                
AGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640102‑2640297

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG