Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,646,493 Δ1 bp 2.2% intergenic (+451/+313) proS → / ← RCAP_rct00032 prolyl‑tRNA synthetase/tRNA‑Ala

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,646,4930C→. 2.2% 2.2 ‑1.8 ‑0.0136intergenic (+451/+313)proS/RCAP_rct00032prolyl‑tRNA synthetase/tRNA‑Ala
Reads supporting (aligned to +/- strand):  new base (1/2):  ref base (99/34):  total (100/36)
Fisher's exact test strand distribution p-value = 1.71e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.98e-01

Only 100 of 156 total aligned reads displayed.

GCAATCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCGGGGC  >  NC_014034/2646393‑2646593
                                                                                                      |                                                                                                     
gCAATCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCCCAACCATGAAGCACGGACC.TTG.c                                                                                                       >  1:522805/1‑101 (MQ=255)
   aTCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.cccc                                                                                                    >  2:246001/1‑101 (MQ=255)
     cccGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGGCGCCCGGGCGCAAGGCGGGCGGCGGCGGAACGACCATGAAGCACGGACA.GTG.ccccgc                                                                                                  >  2:4617821/1‑99 (MQ=37)
     cccGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCCGGCGCAAGCCGGGCGGCGGCGGCACCACCCTGAAGCCCCGACC.TTG.cccccc                                                                                                  >  1:288856/1‑101 (MQ=38)
          cccGCGACACCCCCCCGCCGCCCGGCTTGCGCGGGGGACGCCCGGGCCCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCa                                                                                             <  1:4671333/100‑1 (MQ=38)
            cgcgACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCcga                                                                                           >  2:2510168/1‑101 (MQ=255)
            cgcgACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCcga                                                                                           >  2:1728697/1‑101 (MQ=255)
               gACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.ccccccccccccaggg                                                                                        >  1:2469284/1‑95 (MQ=255)
                 cATCCCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCt                                                                                     <  1:4037586/101‑1 (MQ=255)
                  ccccccccGCCCCCCGGGTTGGCGGGGGGGCGCCCGGGCGCAAGCCGGGCGGGGGCGGCCCAACCATGAAGCCCGGGCC.TTG.CCCCCCCCCCAGAG.TGCTc                                                                                    <  2:3011291/98‑1 (MQ=255)
                   tACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCagaggccgcc                                                                                    >  1:420273/1‑95 (MQ=255)
                   tACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAC.CGCTcc                                                                                   >  1:2883719/1‑101 (MQ=255)
                   cccccccGCGGCCCGGCGGGCGGGCGGGAGGCCCGGGATCCCCCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTcc                                                                                   <  1:141082/99‑1 (MQ=255)
                     cccccGCCCCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGCCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCg                                                                                 <  1:4896211/101‑1 (MQ=255)
                       cccGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCCGAG.GGCTCCCGGc                                                                               >  2:4089947/1‑101 (MQ=255)
                           ccgccCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCg                                                                           >  1:4706140/1‑101 (MQ=255)
                             gccCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGGG.TGGCCCCGGCCCCggg                                                                         >  2:2660156/1‑100 (MQ=255)
                             gccCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCggg                                                                         >  2:4111401/1‑100 (MQ=255)
                             gccCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCggg                                                                         >  2:5361117/1‑100 (MQ=255)
                             gccCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGa                                                                         >  2:5289060/1‑101 (MQ=255)
                               ccGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTGCCCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAg                                                                        >  1:5788133/1‑101 (MQ=255)
                               ccGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAgg                                                                       >  2:2610158/1‑101 (MQ=255)
                               ccGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCCGCCCCGGAgg                                                                       >  2:4864484/1‑101 (MQ=255)
                               ccGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.GGCTCCCCGCCCCGGAgg                                                                       >  1:2141398/1‑101 (MQ=255)
                                  gCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAggggg                                                                    >  1:5779302/1‑101 (MQ=255)
                                  gCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAggggg                                                                    >  1:4444581/1‑101 (MQ=255)
                                    ttGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCa                                                                  >  1:4324100/1‑101 (MQ=255)
                                     tGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.GGCTCCCGCCCCCGGAGGGGGCAt                                                                 >  1:696229/1‑101 (MQ=255)
                                      gcgcggGGCCCGCGCGGCCGCATGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TGG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGTGTGCATc                                                                <  2:1773625/101‑1 (MQ=255)
                                      gcgcgcGGGCCGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGGCC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATc                                                                <  1:2803810/101‑1 (MQ=255)
                                         cgcgGGACGCCGGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGc                                                             <  1:3564937/101‑1 (MQ=255)
                                          gcgGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCa                                                            >  2:2495329/1‑101 (MQ=255)
                                            gggACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAAc                                                          >  1:3792450/1‑101 (MQ=255)
                                            gggACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAAc                                                          >  1:1189034/1‑101 (MQ=255)
                                               aCGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGGGACGCCCCCGCCCCCGG.GGGGGCACCCGCAAcccc                                                       >  1:1374459/1‑99 (MQ=255)
                                                cGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACcgcg                                                      >  1:4151942/1‑101 (MQ=255)
                                                 gCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCgcgc                                                     >  2:3339298/1‑101 (MQ=255)
                                                  cccGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGcc                                                    >  1:4547313/1‑101 (MQ=255)
                                                  cccGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGcc                                                    >  1:2398599/1‑101 (MQ=255)
                                                   ccGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGccc                                                   >  2:5103133/1‑100 (MQ=255)
                                                   ccGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCa                                                   <  2:2845984/101‑1 (MQ=255)
                                                    cGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGcccc                                                  >  2:1212460/1‑99 (MQ=255)
                                                    cGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCac                                                  <  1:2997654/101‑1 (MQ=255)
                                                     gggCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCacg                                                 >  1:5142627/1‑101 (MQ=255)
                                                     gggCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCacg                                                 >  1:3945807/1‑101 (MQ=255)
                                                         gcAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACgg                                             >  1:3688202/1‑101 (MQ=255)
                                                         gcAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.GGCCCCCGCCCCCGGAGGGGGCATCGGCAACCCCGCCaccccgg                                             >  2:3918037/1‑96 (MQ=255)
                                                           aaGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCaagatcgga                                           >  1:302253/1‑96 (MQ=255)
                                                               cGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACcg                                       <  2:4007533/101‑1 (MQ=255)
                                                               cGGGCGGCGGCGACACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.CGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACcg                                       >  1:3143598/1‑101 (MQ=255)
                                                                gggCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGGG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCCCACCGCCGGACACcgg                                      >  2:3703112/1‑100 (MQ=255)
                                                                gggCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGCCACcgc                                      >  2:1395830/1‑101 (MQ=255)
                                                                gggCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACcgc                                      >  1:5420881/1‑101 (MQ=255)
                                                                 ggcggcggcggcACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACcgcg                                     >  1:1735231/1‑101 (MQ=255)
                                                                   cggcggcggcACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGGG.GGCCCCCGCCCCCGGGGGGGCCACCGGCAACCGCGCCCCAACGGACACcgcgct                                   >  1:1772700/1‑99 (MQ=255)
                                                                   cggcggcggcACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.GGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCCCGACGGACACCGCGAt                                   >  2:4800241/1‑101 (MQ=255)
                                                                    ggcggcggcACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.GGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCCCGACGGACACCGCGAtt                                  >  2:1596842/1‑101 (MQ=255)
                                                                    ggcggcggcACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.GGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGAtt                                  >  2:4914285/1‑101 (MQ=255)
                                                                      cggcggcACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGa                                >  2:1501816/1‑101 (MQ=255)
                                                                      cggcggcACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCCCGCCCCGACGGACACCGCGATTGa                                >  1:5069098/1‑101 (MQ=255)
                                                                       ggcggcACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGAt                               >  1:3011014/1‑101 (MQ=255)
                                                                       ggcggcACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAAACGCGCCACGACGGACACCGCGATTGAt                               >  1:4488705/1‑101 (MQ=255)
                                                                        gcggcACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCCGGATTGATc                              >  1:2424801/1‑101 (MQ=255)
                                                                        gcggcACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.GGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATc                              >  1:2019056/1‑101 (MQ=255)
                                                                         cggcACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCCCCCGGCCCCGGAGGGGGCATCGGCAACCCGCCCAAGCCGGAAACCCCGATTGATcc                             >  1:2352131/1‑101 (MQ=255)
                                                                           gcACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGc                           <  2:3206086/101‑1 (MQ=255)
                                                                             acaACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGccc                         >  1:3656480/1‑101 (MQ=255)
                                                                             acaACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCCGCCCCGGGGGGGGCATCGGGAACCGCGCCCCCGCGGGCACCCCGGCTTGTCCGccc                         >  2:3533848/1‑101 (MQ=255)
                                                                              caACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCGGcccc                        >  2:644649/1‑101 (MQ=255)
                                                                              caACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGcccc                        >  2:4210400/1‑101 (MQ=255)
                                                                               aaCCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCg                       >  1:2245777/1‑101 (MQ=255)
                                                                               aaCCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCg                       >  1:5654954/1‑101 (MQ=255)
                                                                               aaCCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCg                       <  2:420229/101‑1 (MQ=255)
                                                                                aCCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCCCGACGGACACCGCGATTGATCCGCCCCgg                      >  1:3072028/1‑101 (MQ=255)
                                                                                aCCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCgg                      >  1:215578/1‑101 (MQ=255)
                                                                                  cATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCTCCCCGGAt                    <  2:2157663/101‑1 (MQ=255)
                                                                                  cATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGAt                    >  2:1013924/1‑101 (MQ=255)
                                                                                  cATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGAt                    >  1:3552702/1‑101 (MQ=255)
                                                                                   aTGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGacg                   >  1:4375138/1‑99 (MQ=255)
                                                                                      aaGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACa                <  2:2883558/101‑1 (MQ=255)
                                                                                      aaGCCCGGACC.TTG..CCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAg               <  1:246034/101‑1 (MQ=255)
                                                                                       aGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAg               <  1:1013999/101‑1 (MQ=255)
                                                                                       aGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAg               <  2:5779007/101‑1 (MQ=255)
                                                                                       aGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAg               <  1:4914880/101‑1 (MQ=255)
                                                                                        gCCCGGACCTTTC.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAg               <  2:5254171/101‑1 (MQ=255)
                                                                                        gCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAgg              >  1:888556/1‑101 (MQ=255)
                                                                                         cccGGCCC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAgga             <  1:4047655/101‑1 (MQ=255)
                                                                                          ccGGACC.TTG.CCCCCCCCCCAAAG.GGGCCCCGGGCCCCGGGGGGGCATCGGCAACCCGCCCCCCCCGGACACCCCGGTTTAACCGCCCCGGATGGCAggag            >  2:4289189/1‑100 (MQ=255)
                                                                                          ccGGACC.TTC.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggac            <  2:3945563/101‑1 (MQ=255)
                                                                                           cGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggacg           >  1:3754806/1‑101 (MQ=255)
                                                                                            ggACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggacgg          <  1:2610307/101‑1 (MQ=255)
                                                                                              gcc.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggacggac        <  1:1501909/100‑1 (MQ=255)
                                                                                              aCC.TTG..CCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGAcc       >  2:4380652/1‑101 (MQ=255)
                                                                                               cc.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGAcc       >  1:143685/1‑101 (MQ=255)
                                                                                                c.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCg      >  1:27490/1‑101 (MQ=255)
                                                                                                c.TTC.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCg      <  2:522755/101‑1 (MQ=255)
                                                                                                  ttG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCgg     >  2:227264/1‑101 (MQ=255)
                                                                                                   tG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGGGATTGATCCGCCCCGGAGGACAGGCCGGACCggg    >  2:5662436/1‑101 (MQ=255)
                                                                                                      ccccccccccAGGG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCGGGGc  >  1:700923/1‑101 (MQ=255)
                                                                                                      ccccccccccAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCGGGGc  <  2:1425622/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
GCAATCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACC.TTG.CCCCCCCCCCAGAG.TGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCGGGGC  >  NC_014034/2646393‑2646593

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG