Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,725,840 Δ1 bp 5.8% intergenic (‑112/+85) cysT ← / ← cysK1 sulfate ABC transporter permease CysT/cysteine synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,725,8400C→. 5.8% 33.2 ‑0.3 ‑0.1224intergenic (‑112/+85)cysT/cysK1sulfate ABC transporter permease CysT/cysteine synthase
Reads supporting (aligned to +/- strand):  new base (9/4):  ref base (130/81):  total (139/85)
Fisher's exact test strand distribution p-value = 7.71e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.69e-01

Only 100 of 275 total aligned reads displayed.

TCCTTCCGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGGCGCCG  >  NC_014034/2725740‑2725940
                                                                                                      |                                                                                                    
tccttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.c                                                                                                      >  1:262351/1‑101 (MQ=255)
 ccttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.cc                                                                                                     <  2:3042239/101‑1 (MQ=255)
 ccttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.cc                                                                                                     <  2:3168674/101‑1 (MQ=255)
   ttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.cccc                                                                                                   <  1:1196237/101‑1 (MQ=255)
   ttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.cccc                                                                                                   >  1:2364698/1‑101 (MQ=255)
      cGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.ccccccc                                                                                                <  1:1560734/101‑1 (MQ=255)
       gTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.cccccccc                                                                                               <  1:1137448/101‑1 (MQ=255)
        tGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.ccccccccc                                                                                              <  1:2737218/101‑1 (MQ=255)
        tGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.ccccccccc                                                                                              <  2:3357896/101‑1 (MQ=255)
         gAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.cccccccccc                                                                                             <  2:3444569/101‑1 (MQ=255)
          aGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.ccccccccccc                                                                                            >  2:1669391/1‑100 (MQ=255)
          aGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCa                                                                                            <  1:608619/101‑1 (MQ=255)
          aGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCa                                                                                            <  2:4914137/101‑1 (MQ=255)
          aGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCa                                                                                            <  2:2797406/101‑1 (MQ=255)
          aGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCa                                                                                            <  1:1623299/101‑1 (MQ=255)
             ttCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTc                                                                                         >  2:2969771/1‑101 (MQ=255)
               cGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTccc                                                                                       >  2:2286110/1‑101 (MQ=255)
               cGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTccc                                                                                       <  2:1235975/101‑1 (MQ=255)
               cGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCC.CTcccc                                                                                      >  1:2587937/1‑101 (MQ=255)
                gTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTcccc                                                                                      >  2:3001965/1‑101 (MQ=255)
                 tCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTccccc                                                                                     <  2:5519923/101‑1 (MQ=255)
                    tttCCAAAATATCCACTGTGTCAGGCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTg                                                                                  <  1:1240607/101‑1 (MQ=255)
                     ttCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATTCCCCCCCCCCCCCTCCCCCggc                                                                                  >  2:3552001/1‑99 (MQ=255)
                       ccAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCg                                                                               >  1:781864/1‑101 (MQ=255)
                       ccAAAATACCCCCTGGGCAAGTCAAAATTAAAAGGGATAAATC.CCCCCTTTTTTGTTGTGAAATAAAAATTTTTTTT.CCCCCCCCCCACTCCCCCGTGCCg                                                                               <  2:3140737/101‑1 (MQ=255)
                        cAAAATTTCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCgg                                                                              <  2:1065896/101‑1 (MQ=255)
                        cAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACCCCCCCGTCCCgg                                                                              >  1:3882020/1‑101 (MQ=255)
                          aaaTATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCgggg                                                                            <  1:5464719/101‑1 (MQ=255)
                          aaaTATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCgggg                                                                            >  2:5023920/1‑101 (MQ=255)
                           aaTATACACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGgc                                                                           >  2:1597306/1‑101 (MQ=255)
                             tatCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGGGCCGGGgcgc                                                                         >  1:4757929/1‑101 (MQ=255)
                              atCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGcc                                                                        >  1:2560134/1‑101 (MQ=255)
                                ccccTGTGTTTGTCCGGATTTAAAGGGATAAAAGTCCCCATTTTTGGTCGTG.AAAGTAGAATTTTATT.CCCCCCCCCCACCCCCCCGTGCCGGGGCGCCAg                                                                      <  2:399897/98‑1 (MQ=255)
                                  aCTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGAc                                                                    >  2:4196241/1‑101 (MQ=255)
                                    tgtgTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGAcca                                                                  >  2:5685273/1‑99 (MQ=255)
                                    tgtgTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGAGCCAGACGa                                                                  >  1:3643742/1‑101 (MQ=255)
                                     gtgtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATTCCCCCCCCCCCACCCCCCCGTGCCGGGGCGCCAAACGa                                                                  >  2:2864787/1‑101 (MQ=255)
                                     gtgtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACCCCCCCGCCCCGGGGCGCCAGACGaa                                                                 >  1:5643678/1‑101 (MQ=255)
                                      tgtCAGTCGAGATTGAAAGGGATAAGTCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCCCCCCCCCCGGCCCGGGCCcccagacaaa                                                                >  1:717913/1‑93 (MQ=255)
                                      tgtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAAACGaaa                                                                >  2:2491456/1‑101 (MQ=255)
                                        tCAGTCGAGATTTAAAGGGATAAATCTCGCCATTCTTTGTCGGT.AATATAAAATTTTTTTCCCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGaaaa                                                               <  1:1669490/101‑1 (MQ=255)
                                        tCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTCCCGGGGCGCCAGACGAAAAg                                                              >  1:3262854/1‑101 (MQ=255)
                                          aGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGc                                                            >  1:4476213/1‑101 (MQ=255)
                                          aGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGc                                                            >  2:2067501/1‑101 (MQ=255)
                                          aGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCCGGCCCGGGCCCCCGGCGAAAAGGc                                                            >  2:477548/1‑101 (MQ=255)
                                           gTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGcc                                                           <  1:4529201/101‑1 (MQ=255)
                                           gTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGccc                                                          <  2:676286/101‑1 (MQ=255)
                                              gagaTTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCTCCCCCAACCCCCCCGTGCCGGGGCGCCAAACGAAAATGcccgc                                                        >  1:2819929/1‑99 (MQ=255)
                                              gagaTTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCccg                                                        >  1:3777723/1‑101 (MQ=255)
                                               agaTTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTCCCGGGGCGCCAGACGAAAAGGCCccgc                                                       >  2:4524383/1‑101 (MQ=255)
                                                 aTTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCccgccg                                                     <  1:1881443/101‑1 (MQ=255)
                                                   tGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGGGCCGGGGCCCCAGACGAAAAGGCCCCGCCggg                                                   >  1:2260207/1‑101 (MQ=255)
                                                   tGAAAGGGATAAATCCCGCCATTCTTGGTCGGT.AATAAAGAATTTTATT.CCCCCCCCCCCCTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCggg                                                   <  1:1784929/101‑1 (MQ=255)
                                                    gAATGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCgggg                                                  >  1:4197419/1‑101 (MQ=255)
                                                      aaGGGATAAATCCCGCCATTCTTGTTCGTG.AATATAAAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGggggc                                               <  2:2587795/101‑1 (MQ=255)
                                                       aGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGggggc                                               >  2:5239057/1‑101 (MQ=255)
                                                        gggATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCCCTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGggggcg                                              <  2:4122953/101‑1 (MQ=255)
                                                         ggATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGggggcgg                                             <  1:2101875/101‑1 (MQ=255)
                                                          gATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGggggcggg                                            <  1:3517445/101‑1 (MQ=255)
                                                             aaaTCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGcc                                         >  1:2302171/1‑101 (MQ=255)
                                                              aaccccGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCt                                        <  2:3582193/96‑1 (MQ=255)
                                                               aTCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCtt                                       >  2:3486598/1‑101 (MQ=255)
                                                               aTCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCtt                                       <  1:1597400/101‑1 (MQ=255)
                                                               aTCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCtt                                       <  1:3002139/101‑1 (MQ=255)
                                                                tctcGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCttt                                      >  1:946612/1‑101 (MQ=255)
                                                                tcccGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCttt                                      <  2:213236/98‑1 (MQ=255)
                                                                 ctcGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCCCTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTg                                     >  1:2914615/1‑101 (MQ=255)
                                                                  tcGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCCCTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGa                                    >  1:3483292/1‑101 (MQ=255)
                                                                  tcGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGa                                    >  1:138052/1‑101 (MQ=255)
                                                                  tcGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCCCCGGGGGGCGGGGCCTTTGa                                    >  1:357697/1‑101 (MQ=255)
                                                                     ccATTCTTGGTCGTG.AATATAGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGa                                <  1:5287480/101‑1 (MQ=255)
                                                                       aTTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAc                               >  1:4147972/1‑101 (MQ=255)
                                                                         tCTTGGTCGTG.AATATAGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAcgcg                            >  1:5172288/1‑101 (MQ=255)
                                                                          cTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAcgcg                            >  1:3080283/1‑101 (MQ=255)
                                                                             ggTCTTA.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCgggg                         <  2:1984551/101‑1 (MQ=255)
                                                                              gTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGa                        >  1:1836912/1‑101 (MQ=255)
                                                                               ttgTG.AATATAGAATTTTGTG.CCCCCCCCCCACCCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGaa                       <  1:4606839/99‑1 (MQ=255)
                                                                                cGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGaaa                      >  1:5048917/1‑101 (MQ=255)
                                                                                 gTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAAc                     <  1:5727633/101‑1 (MQ=255)
                                                                                  tG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAAcc                    >  2:3686323/1‑101 (MQ=255)
                                                                                  gg.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAAcc                    <  2:4250827/100‑1 (MQ=255)
                                                                                   g.AATATAGAATTTTCTT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCg                   <  2:3820913/101‑1 (MQ=255)
                                                                                   g.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCg                   >  2:1959643/1‑101 (MQ=255)
                                                                                   g.AATATAGAATTTTATT.CCCCCCCCCCACCCCCCCGTCCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCg                   >  2:211341/1‑101 (MQ=255)
                                                                                   g.AATATAGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGa                  >  2:4626410/1‑101 (MQ=255)
                                                                                      atatAGAATTTTATC.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGAt                 <  1:4066824/101‑1 (MQ=255)
                                                                                       tataGAATTTTATC.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATc                <  1:2067618/101‑1 (MQ=255)
                                                                                        ataGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATcc               >  2:2719485/1‑100 (MQ=255)
                                                                                          aGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAgg             <  2:5672680/101‑1 (MQ=255)
                                                                                           aatttttTTC.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGc            <  1:3552218/98‑1 (MQ=255)
                                                                                           aaaTTTTTTT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGc            <  2:1337141/100‑1 (MQ=255)
                                                                                               tttATT.CCCCCCCCCCCCTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCggg        <  1:2209098/101‑1 (MQ=255)
                                                                                               tttATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCggg        >  2:3252714/1‑101 (MQ=255)
                                                                                               tttATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGACCCGCCGGGGGGGGGGGCCTTTCAAAGACGCGGGGAAACCGATCAGGCCggg        >  2:921804/1‑101 (MQ=255)
                                                                                                 tATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGGGGGGCCTTTGAAAGAGGGGGGGAAACCGATCAGGCCggggg      >  2:4671045/1‑100 (MQ=255)
                                                                                                 tATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGgc      <  2:5126056/101‑1 (MQ=255)
                                                                                                  aTT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGgcg     >  1:4848741/1‑101 (MQ=255)
                                                                                                  aTT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCCCCGGGGGGGGGGGCCTTTGAAAGACGCGGGGGAACCGATCAGGCCgggggg     >  1:2004540/1‑99 (MQ=255)
                                                                                                  aTT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCCCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATAAGGCCGGGgcc     >  1:251114/1‑100 (MQ=255)
                                                                                                   tt.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGCCGAAAAAGCACCGCCGGGGGGCGGGGCCTTTGAACGACGCGGGGGAGGCGGACAGGCCGGGgcgg    >  2:5144242/1‑100 (MQ=255)
                                                                                                      |                                                                                                    
TCCTTCCGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTG.AATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGACGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGGCGCCG  >  NC_014034/2725740‑2725940

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG