Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 290,303 Δ1 bp 2.1% intergenic (‑36/+14) RCAP_rcc00253 ← / ← RCAP_rcc00254 major facilitator superfamily protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034290,3030G→. 2.1% 7.2 ‑1.0 ‑0.2242intergenic (‑36/+14)RCAP_rcc00253/RCAP_rcc00254major facilitator superfamily protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (128/109):  total (132/110)
Fisher's exact test strand distribution p-value = 3.80e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.91e-01

Only 100 of 296 total aligned reads displayed.

AAGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAGCC  >  NC_014034/290203‑290403
                                                                                                    |                                                                                                    
aaGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCg                                                                                                      >  2:2133803/1‑101 (MQ=255)
   ccAAGCATCAAGATGATGGCGCCACCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgggg                                                                                                   >  1:1818480/1‑101 (MQ=255)
     aaGCATTAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgggggg                                                                                                 <  1:905467/101‑1 (MQ=255)
     aaGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgggggg                                                                                                 >  2:4197844/1‑101 (MQ=255)
        cATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggggggggg                                                                                              >  2:3303172/1‑101 (MQ=255)
         aTCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGc                                                                                             >  1:2206240/1‑101 (MQ=255)
          tCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCt                                                                                            >  1:2418436/1‑101 (MQ=255)
          tCAAGATGATGGCGCCGCCCAAAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCt                                                                                            >  1:3281240/1‑101 (MQ=255)
            aaGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGc                                                                                          <  1:413199/101‑1 (MQ=255)
            aaGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGc                                                                                          <  2:2992780/101‑1 (MQ=255)
              gatgatGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGt                                                                                        >  1:4834742/1‑101 (MQ=255)
               atgatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTc                                                                                       <  2:4102365/101‑1 (MQ=255)
                tgatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCa                                                                                      >  1:756594/1‑101 (MQ=255)
                tgatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCa                                                                                      <  1:4678896/101‑1 (MQ=255)
                 gatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCaa                                                                                     <  1:3815156/101‑1 (MQ=255)
                 gatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCaa                                                                                     >  2:5725539/1‑101 (MQ=255)
                 gatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGGCaa                                                                                     >  1:4577639/1‑101 (MQ=255)
                 gagggCCCCGCCCACAGGGAGGGGGGGGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCaa                                                                                     <  2:5496484/98‑1 (MQ=255)
                      cgccgcCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGt                                                                                >  2:4754511/1‑101 (MQ=255)
                       gccgccCACAGGGACGGGGGTGATCAGGCGGGTCTGGGTCATTGGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTc                                                                               <  2:5523817/101‑1 (MQ=255)
                       gccgccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTc                                                                               >  1:3473102/1‑101 (MQ=255)
                       gccgccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTc                                                                               <  1:426879/101‑1 (MQ=255)
                       gccgccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTc                                                                               <  1:2376326/101‑1 (MQ=255)
                         cgccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGGCAATCTGTcca                                                                             >  2:5068186/1‑99 (MQ=255)
                         cgccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCCGCGTCAATCTGTCGa                                                                             >  2:79900/1‑101 (MQ=255)
                          gccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTGCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGAt                                                                            >  2:5000801/1‑101 (MQ=255)
                          gccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGAt                                                                            >  1:2663262/1‑101 (MQ=255)
                           cccACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGAtt                                                                           <  2:4497942/101‑1 (MQ=255)
                           cccACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGAtt                                                                           <  1:1010069/101‑1 (MQ=255)
                            ccAGAAGGCGGGGGTTGCCCAGTCGGGGCCGGGCGATTTTGCCTCCTTCGCAAGGCGGCTAGTTCGCGATGCGGGGGGGGGCTGCGTCAATCTGTCGATtg                                                                          <  2:3517813/101‑1 (MQ=255)
                             cacaAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtgt                                                                         <  2:5649871/101‑1 (MQ=255)
                               caAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGc                                                                       <  2:3723957/101‑1 (MQ=255)
                               caAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGc                                                                       >  2:939179/1‑101 (MQ=255)
                               caAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGc                                                                       >  1:3553388/1‑101 (MQ=255)
                                aaGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGcc                                                                      >  1:3346198/1‑101 (MQ=255)
                                 aGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGccc                                                                     >  1:2167756/1‑101 (MQ=255)
                                 aGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGccc                                                                     <  1:5529805/101‑1 (MQ=255)
                                  ggACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGcccc                                                                    <  1:5669919/101‑1 (MQ=255)
                                   gACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCg                                                                   <  1:5641436/101‑1 (MQ=255)
                                     cGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAAc                                                                >  2:1155646/1‑101 (MQ=255)
                                      gggggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAAc                                                                <  1:1767746/101‑1 (MQ=255)
                                       ggggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACa                                                               >  2:136058/1‑101 (MQ=255)
                                            gATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcgc                                                          <  2:3789875/101‑1 (MQ=255)
                                              tCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCgcgc                                                        <  2:4565032/101‑1 (MQ=255)
                                               cAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgcc                                                       >  2:5560692/1‑101 (MQ=255)
                                               cAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgcc                                                       >  1:1224706/1‑101 (MQ=255)
                                               cAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgcc                                                       <  1:5283944/101‑1 (MQ=255)
                                               cAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgcc                                                       <  1:3891835/101‑1 (MQ=255)
                                               cAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGCCAATCTGTCGATTGTGCCCCGAACAATCGcgcc                                                       >  1:4940027/1‑101 (MQ=255)
                                                   ggggTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgccg                                                   <  2:3614082/100‑1 (MQ=255)
                                                    gggTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgccgc                                                  <  2:1013032/101‑1 (MQ=255)
                                                     ggTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCgccgccgcc                                                 >  1:584200/1‑101 (MQ=255)
                                                     ggTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCgccgccgcc                                                 >  1:1044617/1‑101 (MQ=255)
                                                      gTCTGTGTAATTTGCCCACCCTCGCACGGCGGCGAGTTCCCCATGGGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCt                                                <  1:3785760/101‑1 (MQ=255)
                                                      gTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCt                                                <  2:1952559/101‑1 (MQ=255)
                                                       tCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGACTGCCCCCCGAACAATCGCGCCGCCGCCtc                                               >  2:5226172/1‑100 (MQ=255)
                                                         tGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGcc                                             >  2:557951/1‑101 (MQ=255)
                                                          gggTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGccc                                            >  2:3198919/1‑101 (MQ=255)
                                                          gggTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGccc                                            >  1:1482216/1‑101 (MQ=255)
                                                             tCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCg                                        >  1:5086361/1‑101 (MQ=255)
                                                              cATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCg                                        >  2:3941025/1‑101 (MQ=255)
                                                                tttGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAc                                      <  1:4905381/101‑1 (MQ=255)
                                                                tttGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAc                                      <  1:5145570/101‑1 (MQ=255)
                                                                 ttGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGTGCCGCCGCCTGCCCGGCGAcg                                     >  2:255/1‑101 (MQ=255)
                                                                   ggTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcgcg                                   <  2:4655612/101‑1 (MQ=255)
                                                                   ggTCTCCCCCGAAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcgcg                                   <  2:2206118/101‑1 (MQ=255)
                                                                    gTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCgg                                  <  2:1263552/101‑1 (MQ=255)
                                                                    gTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCgg                                  >  1:2506638/1‑101 (MQ=255)
                                                                     tctcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCTCCGCCGCCTGCCCGGCGACGCGGc                                 >  1:4717775/1‑101 (MQ=255)
                                                                     tctcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCCGCGTCAATCTGTCGATTGTGCCCCGCACACTCGCGCCGCCGCCTCCCCGGCCGcgcgcc                                 >  1:1014098/1‑99 (MQ=255)
                                                                      ctcCCTCGCAAGGGGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGcc                                <  1:424119/101‑1 (MQ=255)
                                                                       tcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGccc                               <  1:3182824/101‑1 (MQ=255)
                                                                       tcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGccc                               >  2:435410/1‑101 (MQ=255)
                                                                         ccTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCgg                             >  1:2119919/1‑100 (MQ=255)
                                                                         ccTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCgc                             >  1:1143809/1‑100 (MQ=255)
                                                                           tCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCg                           <  1:2795302/101‑1 (MQ=255)
                                                                           tCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCg                           >  2:1824016/1‑101 (MQ=255)
                                                                              cggggcggcCGGGGCGGCAGGGGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCa                        <  2:2050468/98‑1 (MQ=255)
                                                                              cAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCa                        >  1:3968959/1‑101 (MQ=255)
                                                                               aaGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGGACAATCGCGCCGCCGCCTGGCCGGCGACGCGGCCCGTCGccgg                       >  2:3419465/1‑99 (MQ=255)
                                                                               aaGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAg                       >  1:1913823/1‑101 (MQ=255)
                                                                                aGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGCCGCGGCCCGTCGCCAGa                      >  1:1320097/1‑101 (MQ=255)
                                                                                aGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGa                      >  2:5328775/1‑101 (MQ=255)
                                                                                 ggcggcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGAc                     <  2:4936845/101‑1 (MQ=255)
                                                                                 ggcggcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGAc                     >  2:3220341/1‑101 (MQ=255)
                                                                                  gcggcCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAg                   <  1:4429902/101‑1 (MQ=255)
                                                                                   cggcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAg                   <  2:4926259/101‑1 (MQ=255)
                                                                                      ccAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACCGCCg                >  2:605238/1‑101 (MQ=255)
                                                                                      ccAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCg                >  1:1712394/1‑101 (MQ=255)
                                                                                       cAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCgg               >  2:1167646/1‑101 (MQ=255)
                                                                                       cAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCgg               <  1:3656496/101‑1 (MQ=255)
                                                                                        aGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGt              >  2:3749782/1‑101 (MQ=255)
                                                                                         gTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTc             <  1:701160/101‑1 (MQ=255)
                                                                                           tCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCCGACAGCCGGTcag           >  2:669527/1‑101 (MQ=255)
                                                                                           tCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACCGCCGGGcag           >  2:1052940/1‑101 (MQ=255)
                                                                                           tCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcag           >  2:3873350/1‑101 (MQ=255)
                                                                                             gCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcagca         >  2:328177/1‑101 (MQ=255)
                                                                                                 ggCGGGGGGGGCCTGCGTCAATCTGTCGATTGGGCCCCGAACAATCCCGCCGCGGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATa     <  2:1730045/100‑1 (MQ=255)
                                                                                                  gCGGGGGGGGACTGCGTCAATCTGTCGATTGTGCCACGAACAATCGCGCCGCAGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAg    <  1:957196/101‑1 (MQ=255)
                                                                                                    gggggggggCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAgcc  <  1:2052966/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
AAGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAGCC  >  NC_014034/290203‑290403

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG