Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,791 Δ1 bp 2.6% intergenic (‑94/+69) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7910G→. 2.6% 3.5 0.0 0.0155intergenic (‑94/+69)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (59/90):  total (63/92)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

GGCTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGC  >  NC_014034/3439691‑3439892
                                                                                                        |                                                                                                          
ggcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGC.GAG.A.TAAAg                                                                                                            >  2:22007/1‑101 (MQ=255)
 gcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGC.AAG.A.TAAAgg                                                                                                           >  1:222298/1‑101 (MQ=255)
 gcTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAgg                                                                                                           <  1:3884961/101‑1 (MQ=255)
  cTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAggg                                                                                                          <  1:3764367/101‑1 (MQ=255)
   tCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAgggg                                                                                                         <  2:2363332/101‑1 (MQ=255)
     aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGTTGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGgc.t                                                                                                      <  2:2409794/101‑1 (MQ=255)
     aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGgc.t                                                                                                      <  1:1188462/101‑1 (MQ=255)
     aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCAGC.GGGGGTATTTGGGGG.CAG.A.AAAAgggggt                                                                                                       >  2:811677/1‑99 (MQ=255)
       ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGgc.ttt                                                                                                    <  2:4301826/101‑1 (MQ=255)
       ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGgc.ttt                                                                                                    <  2:4450803/101‑1 (MQ=255)
          ggcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGAATTTGGGG.GAG.A.TAAAAGGgg.ttttc.t                                                                                                >  1:2309552/1‑101 (MQ=255)
           gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGgc.tttgc.tt                                                                                               <  2:3177364/101‑1 (MQ=255)
            cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGgc.tttgc.ttt                                                                                              <  2:4663039/101‑1 (MQ=255)
            cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGgc.tttgc.ttt                                                                                              <  1:312679/101‑1 (MQ=255)
            cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGAGAGTTGGGGC.GAG.AGTAAAGGgg..gttgc.ttt                                                                                              >  2:4113304/1‑101 (MQ=255)
             gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGG.AAGAA.AAAAGGGgg.tttgt.ttt                                                                                              >  1:5289046/1‑101 (MQ=255)
             gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.tttt                                                                                             <  1:2465738/101‑1 (MQ=255)
              aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGG.CAG.A.GAAAAGGGG.TTTGG.ttttt                                                                                            >  1:1005856/1‑100 (MQ=255)
              aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTa                                                                                            <  1:4188290/101‑1 (MQ=255)
               cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTAt                                                                                           <  1:2045162/101‑1 (MQ=255)
                ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATc                                                                                          <  1:2284241/101‑1 (MQ=255)
                 cTAGACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.t                                                                                        <  2:210976/101‑1 (MQ=255)
                  tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGG.AAG.A.TAAAGGGGG.TTTGC.TTTTTTC.tt                                                                                       >  2:5563040/1‑101 (MQ=255)
                   acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.ttt                                                                                      <  1:5484030/101‑1 (MQ=255)
                     acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTcc                                                                                    <  2:2278769/101‑1 (MQ=255)
                     acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTCGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTcc                                                                                    <  1:4266590/101‑1 (MQ=255)
                      cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTccc                                                                                   <  2:2084486/101‑1 (MQ=255)
                         cgcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTGGGGG.GAG.A.TAAAAGGGG.GTTGT.TTTTTTT.TTTtcccca                                                                                >  2:67392/1‑99 (MQ=255)
                         cgcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCaa                                                                                <  2:49186/101‑1 (MQ=255)
                           cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.GAG.A.AAAAAGGGC.TTTGC.TTTTTTCTTTTCCCCaaa                                                                               >  1:2781781/1‑101 (MQ=38)
                           cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAat                                                                              <  1:1099154/101‑1 (MQ=255)
                           cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAat                                                                              <  1:2356317/101‑1 (MQ=255)
                            gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGG.CAG.A.GAAAAGGGG.GTTGG.TTTTTTT.TTTCCCCaaaaa                                                                             >  1:5719132/1‑99 (MQ=255)
                            gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGAAATTTTGGGG.AAG.A.TAAAGGGGG.TTTGG.TTTTTTT.TTTTCCCCAata                                                                             >  1:578270/1‑101 (MQ=255)
                             aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGG.AAG.A.TAAAAGGGG.TTTGC.TTTTTTT.TTTTCCCAAatat                                                                            >  2:3319628/1‑101 (MQ=255)
                              ccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATc                                                                           <  1:55675/101‑1 (MQ=255)
                               cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGCAAAA.A.AAAAGGGGT.TTTGT.TTTTTTT.TTTCCCCaatattc                                                                           >  1:5154811/1‑96 (MQ=255)
                               cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGG.GAG.A.AAAAAGGGG.TTTGCTTTTTTTT.TTTCCCCaaaattc                                                                           >  1:4289374/1‑97 (MQ=255)
                               cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATcc                                                                          <  2:4434519/101‑1 (MQ=255)
                               cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATcc                                                                          <  1:3478639/101‑1 (MQ=255)
                                aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATccc                                                                         <  1:4507855/101‑1 (MQ=255)
                                 gCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCg                                                                        <  1:1341562/101‑1 (MQ=255)
                                  cTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGG.AAG.A.TAAAGGGGG.TTTGC.TTTTATT.TTTTCCCCAAAATCCCgg                                                                       >  2:619414/1‑101 (MQ=255)
                                   tttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGG.AAA.A.AAAAAGGGC.TTTTT.TTTTTTT.TTTTTCCCAATATCCCcgg                                                                      >  1:919418/1‑101 (MQ=255)
                                     tCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGG.GAG.A.TAAAAGGGCTTTTGT.TTTTTTT.TTTCCCCAAA.ATCCCcgggg                                                                    >  2:3116321/1‑101 (MQ=255)
                                      cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTGGGGC.AAG.A.TAAAAGGGC.TTTGC.TTTTTTC.TTTTCCCCAATATCCCgggggg                                                                   >  2:4414719/1‑100 (MQ=37)
                                      cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGt                                                                   <  1:5525741/101‑1 (MQ=255)
                                      cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGAGATTTTGGGG.AAG.A.AAAAAGGGG.TTTGC.TTTTTTT.TTTTCCCCAATATTCCgggggg                                                                   >  1:685721/1‑100 (MQ=255)
                                       gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGC.AAG.A.TAAAAGGGC.CTTGC.TTTTTTT.TTTCCCCCAATATACCggggggg                                                                  >  2:4579958/1‑99 (MQ=255)
                                       gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTg                                                                  <  2:2518283/101‑1 (MQ=255)
                                       gAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTg                                                                  <  2:4463786/101‑1 (MQ=255)
                                        aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGc                                                                 <  2:5637183/101‑1 (MQ=255)
                                         aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGGATTTTGGGC.GAG.A.AAAAAGGGC.TTTGCTTTTTTTT.TTTCCCCAAAATTCCCgggggggg                                                                 >  1:381717/1‑98 (MQ=255)
                                         aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..g                                                              <  1:5790582/101‑1 (MQ=255)
                                          aGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGAAATTTGGGG..AAG.A.TAAAAGGGC.TTTGC.TTTTTTT.TTTTCCCCAATATCCCGGGGGGGC..gg                                                             >  1:2850441/1‑101 (MQ=255)
                                            aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..gggg                                                           <  2:5122719/101‑1 (MQ=255)
                                               ggAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGGTATTTTGGGG.AAG.A.AAAAAGGGG.GTTGC.TTTTTTT.TTTTCCCAAATATCCCCGGGGGGG..ggggggg                                                        >  1:3975066/1‑99 (MQ=255)
                                               ggAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTTGGGG.GAG.A.AAAAAAGGG.GTTGA.TTTTTTT.TTTTTCCCACTAACCCCGGGGGGG..GGGGGcg                                                        >  1:1218659/1‑100 (MQ=255)
                                               ggAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCa                                                        <  2:2635160/101‑1 (MQ=255)
                                                gAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCag                                                       <  1:180563/101‑1 (MQ=255)
                                                 aaTTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCaga                                                      <  1:263735/101‑1 (MQ=255)
                                                   ttCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTTGGGC.AAG.A.TAAAGGGGC.TTTTC.TTTTTTC.TTTTCCCCAATATCCCCGGGGTGG..ggggggcgagg                                                    >  2:408253/1‑95 (MQ=25)
                                                    tCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGcc                                                   <  2:1735106/101‑1 (MQ=255)
                                                    gcgcccTGGGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAGGGGGT.TTTGT.TTTTTTC.TTCCCCAAAATATCCCGGGGGTGC..GGGGGCAGAGcc                                                   <  2:1966955/97‑1 (MQ=21)
                                                      tgCCTGTGGCCGAGGCCTCCGGG.GTGGATATTCGGTGC.AAC.A.TCAAGGGTC.TTGCC.TTTTATC.GTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGCGcccc                                                 <  2:746515/100‑1 (MQ=16)
                                                      ggCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGG.AAG.A.TAAAAGGGG.TTTGC.TTTTTTC.TTTCCCCAAATATCCCGGGGGGGC..GGGGGGAGGGgccc                                                 >  1:3526358/1‑101 (MQ=25)
                                                      ggCCTGCGGCCGATGCCTCCGGC.AGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGcccc                                                 <  2:5699008/101‑1 (MQ=255)
                                                         cTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGG.AAG.A.TAAAAGGGG.TTTGC.TTTTTTC.TTTCCCCCAATATCCCCGGGGGGC..GGGGGCAGAGccccccc                                              >  2:5355924/1‑99 (MQ=25)
                                                         cTGCGGCCGATCCCTCCGGC.GGGTATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGACCCCCcgc                                              <  2:5404681/101‑1 (MQ=255)
                                                            ctttcGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TGAA.GCCC.ATCCC.TTTAATC.TTGCCCCAAATATCCCGGGGGACCCGGGGGGCAGCGccccccgcc                                            <  2:1873158/97‑5 (MQ=21)
                                                                cGATGCCTCCGGC.GGGGATATTTTGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGGGC..GGGGGGAGAGCCCCcgccgccccg                                       >  1:2529079/1‑94 (MQ=21)
                                                                cGATGCCTCCGGC.GGGGAAATTTTGGGC.AAG.A.TAAAGGGGG.TTTTG.TTTTATC.TTTCCCCAAATATCCCGGGGGGGC..GGGGGCAGAGCCCCcgccgccacg                                       >  2:5531468/1‑94 (MQ=21)
                                                                      cTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAc                               >  2:1537611/1‑101 (MQ=255)
                                                                        ccGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCC.C.CCGCCCCGGGTCAcgc                             >  2:3526761/1‑101 (MQ=18)
                                                                           gc.ggGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgccgc                          >  1:4692927/1‑101 (MQ=255)
                                                                            c.ggGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg                         >  2:3913489/1‑101 (MQ=255)
                                                                            c.ggGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg                         >  2:1659115/1‑101 (MQ=255)
                                                                            c.ggGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg                         >  1:328963/1‑101 (MQ=255)
                                                                              ggggATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                        >  1:1369681/1‑101 (MQ=255)
                                                                              ggggATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                        >  2:4969395/1‑101 (MQ=255)
                                                                               gggATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                       >  1:5213294/1‑101 (MQ=255)
                                                                               gggATATTTGGGGC.AAG.A.TAAAGGGGC.TTTCC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                       >  1:5144517/1‑101 (MQ=255)
                                                                                ggATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGCACACGCGGCGAcc                      >  2:1440716/1‑101 (MQ=17)
                                                                                 gATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCg                     >  1:164790/1‑101 (MQ=255)
                                                                                  atatTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGt                    >  2:431255/1‑101 (MQ=255)
                                                                                   tatTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtc                   >  2:1689208/1‑101 (MQ=255)
                                                                                    atTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                  >  2:2759915/1‑101 (MQ=255)
                                                                                     tttGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtctc                 >  2:5059598/1‑101 (MQ=255)
                                                                                     tttGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtctc                 >  1:48469/1‑101 (MQ=255)
                                                                                        ggggC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg              >  2:3468972/1‑101 (MQ=255)
                                                                                             gggaaaaaaaaGGGGT.CTTGT.TTTTTTT.TTTCCCCAAA.ATCCCCGGGGGGT..GGGGGGGAAGACCCCCAGCCGCCACGGTTCACGCCGCGACCGTCTCCggg             <  1:1939306/93‑1 (MQ=255)
                                                                                              aaG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg         >  2:101627/1‑101 (MQ=255)
                                                                                               aG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc        >  2:1158776/1‑101 (MQ=255)
                                                                                                 aaaatgaGGGGT.TTTTC.TTTTTTA.TCTCCCCAAAATTCGGGGGGGGGT..GGGGGGCCGCCCCCCCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc          <  2:1503082/95‑1 (MQ=255)
                                                                                                  a.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa      >  1:5511108/1‑101 (MQ=255)
                                                                                                  a.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa      >  1:5598530/1‑101 (MQ=255)
                                                                                                    tAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCGCGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg     >  1:4965843/1‑101 (MQ=255)
                                                                                                    tAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg     >  2:5709579/1‑101 (MQ=255)
                                                                                                     aaaGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAgc    >  1:4486715/1‑101 (MQ=255)
                                                                                                       aGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc  >  2:587516/1‑101 (MQ=255)
                                                                                                        |                                                                                                          
GGCTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGC.GGGGATATTTGGGGC.AAG.A.TAAAGGGGC.TTTGC.TTTTATC.TTTCCCCAAATATCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGC  >  NC_014034/3439691‑3439892

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG