Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,469 Δ1 bp 3.5% intergenic (+72/+76) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4690T→. 3.5% 15.0 ‑1.9 ‑0.9231intergenic (+72/+76)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/2):  ref base (100/123):  total (106/125)
Fisher's exact test strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.24e-01

Only 100 of 257 total aligned reads displayed.

GCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCG  >  NC_014034/432370‑432568
                                                                                                     |                                                                                                    
gCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.At                                                                                                      <  2:1792117/101‑1 (MQ=255)
  tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATcc                                                                                                    <  2:2640957/101‑1 (MQ=255)
   ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGGCCCCCGTCCTGCGGACTCCACCCGGGATATTTGACGCAAGATGAAAGCCG.ATCCg                                                                                                   >  2:1435540/1‑101 (MQ=21)
    tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCgg                                                                                                  <  2:3724479/101‑1 (MQ=255)
    tCGGCGGCGGAGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCgg                                                                                                  <  1:5488654/101‑1 (MQ=255)
     cggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCcgg                                                                                                 >  1:4454289/1‑101 (MQ=255)
       gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCt                                                                                               >  1:245205/1‑101 (MQ=255)
        cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCtt                                                                                              >  1:1616403/1‑101 (MQ=255)
         ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTc                                                                                             <  1:3723534/101‑1 (MQ=255)
         ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTc                                                                                             <  2:5337111/101‑1 (MQ=255)
         ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTc                                                                                             <  2:4410641/101‑1 (MQ=255)
           cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGt                                                                                           <  2:4833880/101‑1 (MQ=255)
             gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGttt                                                                                         <  1:4640541/101‑1 (MQ=255)
              cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTc                                                                                        <  1:331890/101‑1 (MQ=255)
              cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTc                                                                                        <  2:5083619/101‑1 (MQ=255)
               gggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCa                                                                                       <  1:3521478/101‑1 (MQ=255)
               gggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCa                                                                                       >  1:2702692/1‑101 (MQ=255)
                ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCAt                                                                                      <  2:59371/101‑1 (MQ=255)
                  ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCt                                                                                    <  1:5462761/101‑1 (MQ=255)
                   cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCtt                                                                                   >  1:4331278/1‑101 (MQ=255)
                   cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCA.ATCCGGGCTTCGTTTCATCtt                                                                                   <  1:4265961/101‑1 (MQ=255)
                      gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGcc                                                                                >  2:2341191/1‑101 (MQ=255)
                         tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCa                                                                             >  1:2663333/1‑101 (MQ=255)
                          gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCaa                                                                            >  1:1304300/1‑101 (MQ=255)
                          gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGGTATTTGAGGCAAGATGAAAACCG.ATCCGGGGTTCGTTTCTTTTTTccccca                                                                            >  1:3946796/1‑99 (MQ=17)
                           aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCaaa                                                                           <  2:1015197/101‑1 (MQ=255)
                            ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                          <  1:91761/101‑1 (MQ=255)
                            ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                          <  2:4677033/101‑1 (MQ=255)
                             gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAata                                                                         <  1:964165/101‑1 (MQ=255)
                              gcGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGTGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                                                        <  2:3785604/100‑1 (MQ=255)
                                gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGAATTTGGGTCAAGATGAAAGCCG.ATGCGGGCTTCGGTTCATCTTGCCCCCAATAA.cc                                                                     >  1:3983753/1‑101 (MQ=18)
                                gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.cc                                                                     <  2:735686/101‑1 (MQ=255)
                                gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATAGTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTTATCTTGGCCCGAATAT.cc                                                                     >  2:1591596/1‑101 (MQ=18)
                                 ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATAT.CCCAGGGGTGAAtt                                                         >  2:4111636/5‑101 (MQ=2)
                                 ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.ccc                                                                    <  1:1872491/101‑1 (MQ=255)
                                 ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.ccc                                                                    <  1:2231118/101‑1 (MQ=255)
                                   cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCgg                                                                  >  2:4528731/1‑101 (MQ=255)
                                   cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCgg                                                                  <  2:2820545/101‑1 (MQ=255)
                                   cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCgg                                                                  >  1:1485863/1‑101 (MQ=255)
                                   acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATAT.CCCAGGGGTGAAttgg                                                       >  1:5550287/3‑99 (MQ=2)
                                    cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATAT.CCCAGGGGTGAAttggc                                                      >  2:135129/2‑98 (MQ=2)
                                     ggggggGCCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCgggg                                                                <  1:767491/101‑1 (MQ=25)
                                     ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCCAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCACATAT.CCCgggg                                                                >  1:466918/1‑101 (MQ=25)
                                     ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCgggg                                                                >  1:2562656/1‑101 (MQ=255)
                                      gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCggggg                                                               >  2:4166106/1‑101 (MQ=255)
                                        gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAAGCG.ATCCGGGCTTCGTTTCATCTTGCCCCCAATAT.CCCcgggggg                                                             >  1:1046620/1‑99 (MQ=21)
                                         ggCTCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGa                                                            <  2:1327564/101‑1 (MQ=255)
                                         ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCCAGATGAAAGCCG.ATCCGGGCTNCGNTNCNTCTTGCCCCCANTAT.CCCGGGGGGGa                                                            >  1:4984814/1‑101 (MQ=21)
                                              tGCCCCCCGTCCTGCGGACTCCCCCCGGGGTATTTTAGGGAAAATGAAAACCG.GACCCGGGTTCGGTTTCTCTTTCCCCCCATATCCCCGGGGGGGAAttt                                                        >  1:45685/1‑101 (MQ=2)
                                              tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAAtttt                                                       <  2:5474506/101‑1 (MQ=255)
                                              tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAAtttt                                                       <  1:5011224/101‑1 (MQ=255)
                                              tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAAtttt                                                       >  2:2471759/1‑101 (MQ=255)
                                                ccccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTgg                                                     <  1:3177913/101‑1 (MQ=255)
                                                ccccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTgg                                                     >  1:1761281/1‑101 (MQ=255)
                                                  ccccGTCCTGCGGACTCCCCCCGGGGTATTTTAGGGAAGATGAAAGACG.ATCCCGGCTTCGTTTTATCTTTCCCCCAACAT.CCCCGGGGGGAATTTTgggc                                                   >  2:1136278/1‑99 (MQ=16)
                                                  ccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGgcg                                                   <  2:1991723/101‑1 (MQ=255)
                                                  ccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGgcg                                                   <  2:5673966/101‑1 (MQ=255)
                                                   cccGTCCTGCGGACTCCCCCCGGGGAATTTTAGGGAAAAAGAAAACCGAACCCGGGCTTCGTTTCTTTTTCCCCCAAAATC.CCCGGGGGGGATttttttgc                                                   >  1:16629/1‑97 (MQ=255)
                                                   cccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGgcgc                                                  <  2:4025565/101‑1 (MQ=255)
                                                     cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAg                                                >  1:3652063/5‑101 (MQ=255)
                                                      gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGc                                               <  2:2874959/98‑1 (MQ=255)
                                                      gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGc                                               <  2:692784/98‑1 (MQ=255)
                                                        ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCa                                             >  2:4313518/2‑101 (MQ=255)
                                                        ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGAGGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCa                                             <  2:3523564/100‑1 (MQ=255)
                                                         cTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCaa                                            <  2:4873870/101‑1 (MQ=255)
                                                          tGCGGCCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCaaa                                           <  2:3983509/101‑1 (MQ=255)
                                                               aCTCCCCCCGGGATATTTCAGGCAAGATGAAAGGCG.ATCCGGGCTTGGTTTCATCCTGCCCCAAATAT.CCCGGGGGGGAATTTTGGCGCAGCCAAAAGAgg                                      >  2:5183687/1‑101 (MQ=18)
                                                                  ccccccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggggg                                   <  1:4166356/101‑1 (MQ=255)
                                                                    ccccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                 <  2:3313220/101‑1 (MQ=255)
                                                                    ccccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                 >  2:2404996/1‑101 (MQ=255)
                                                                    ccccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                 <  1:656074/101‑1 (MQ=255)
                                                                     cccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGTCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGTGGCAg                                <  2:1540510/101‑1 (MQ=255)
                                                                      ccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                               <  2:563656/101‑1 (MQ=255)
                                                                      ccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                               <  2:213266/101‑1 (MQ=255)
                                                                        gggATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                             >  1:2400090/1‑101 (MQ=255)
                                                                         ggATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                            >  1:5280629/1‑101 (MQ=255)
                                                                         ggATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                            <  1:3037461/101‑1 (MQ=255)
                                                                           atatTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                          <  2:1761182/101‑1 (MQ=255)
                                                                           atatTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCAGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                          >  1:2095129/1‑101 (MQ=255)
                                                                            tatTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                         <  2:4556665/101‑1 (MQ=255)
                                                                             atTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                        >  1:2022021/1‑101 (MQ=255)
                                                                               ttGAGGCAAGATGAAAGCCG.GTCCGGGCTTCGTTTCATCTTGCCCCAAATTT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                      <  1:2412119/101‑1 (MQ=255)
                                                                                  aGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTTAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                   <  2:1690052/101‑1 (MQ=255)
                                                                                   ggCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                  <  1:4493856/101‑1 (MQ=255)
                                                                                   ggCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                  >  1:3183576/1‑101 (MQ=255)
                                                                                    gCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                 <  1:1560460/101‑1 (MQ=255)
                                                                                    gCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                 >  2:4994585/1‑101 (MQ=255)
                                                                                      aaGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg               <  1:5561719/101‑1 (MQ=255)
                                                                                       aGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg              >  2:2697940/1‑101 (MQ=255)
                                                                                         aTGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAACGAGGGGGCACCGCCCCCTATCCCcccgccg            >  2:1887502/1‑101 (MQ=25)
                                                                                           gAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg          >  1:5083847/1‑101 (MQ=255)
                                                                                           gAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg          >  2:2991101/1‑101 (MQ=255)
                                                                                             aaGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggccgcgc        >  1:1027197/1‑96 (MQ=255)
                                                                                             aaGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc        >  1:5770408/1‑101 (MQ=255)
                                                                                             aaGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGGGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc        >  1:5579080/1‑101 (MQ=255)
                                                                                              aGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg       <  2:3137237/101‑1 (MQ=255)
                                                                                                 cG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcg    >  2:3427831/1‑101 (MQ=255)
                                                                                                  g.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc   >  1:1369795/1‑101 (MQ=255)
                                                                                                  g.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc   >  1:4496320/1‑101 (MQ=255)
                                                                                                    aTCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg  >  2:3605901/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCG  >  NC_014034/432370‑432568

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG