Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,471 Δ1 bp 3.5% intergenic (+74/+74) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4710C→. 3.5% 16.6 ‑1.9 ‑0.5230intergenic (+74/+74)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/2):  ref base (100/122):  total (106/124)
Fisher's exact test strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.00e-01

Only 100 of 260 total aligned reads displayed.

TTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCG  >  NC_014034/432372‑432571
                                                                                                     |                                                                                                     
tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATcc                                                                                                       <  2:2640957/101‑1 (MQ=255)
  tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCgg                                                                                                     <  2:3747752/101‑1 (MQ=255)
   cggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCcgg                                                                                                    >  1:4454289/1‑101 (MQ=255)
     gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCt                                                                                                  >  1:245205/1‑101 (MQ=255)
      cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCtt                                                                                                 <  1:995372/101‑1 (MQ=255)
       ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTc                                                                                                <  1:3723534/101‑1 (MQ=255)
        gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCg                                                                                               >  1:1690151/1‑101 (MQ=255)
         cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGATGCAATATGAAAGCCG.AACCGGGCTTCGt                                                                                              >  2:421536/1‑101 (MQ=21)
         cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGt                                                                                              <  2:4833880/101‑1 (MQ=255)
         cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGt                                                                                              <  1:5505020/101‑1 (MQ=255)
           gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGttt                                                                                            <  1:4640541/101‑1 (MQ=255)
            cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTc                                                                                           <  2:2890496/101‑1 (MQ=255)
            cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTc                                                                                           <  2:5083619/101‑1 (MQ=255)
             gggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCa                                                                                          <  1:3521478/101‑1 (MQ=255)
              ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCAt                                                                                         <  2:59371/101‑1 (MQ=255)
               gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGACG.ATCCGGGCTTCGTTTCATc                                                                                        >  2:1342194/1‑101 (MQ=255)
                ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCt                                                                                       <  1:3978382/101‑1 (MQ=255)
                ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCt                                                                                       <  1:1428640/101‑1 (MQ=255)
                 cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCA.ATCCGGGCTTCGTTTCATCtt                                                                                      <  1:4265961/101‑1 (MQ=255)
                    gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGcc                                                                                   >  2:2341191/1‑101 (MQ=255)
                     ccTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGccc                                                                                  <  1:5473219/101‑1 (MQ=255)
                      cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGcccc                                                                                 >  1:3313420/1‑101 (MQ=255)
                       tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCa                                                                                >  1:2663333/1‑101 (MQ=255)
                            gcGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGTGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                                                           <  2:3785604/100‑1 (MQ=255)
                            ccGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                                                           <  2:4427269/101‑1 (MQ=255)
                             cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.c                                                                         <  2:14140/101‑1 (MQ=255)
                             cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.c                                                                         <  2:4545255/101‑1 (MQ=255)
                              gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGAATTTGGGTCAAGATGAAAGCCG.ATGCGGGCTTCGGTTCATCTTGCCCCCAATAA.cc                                                                        >  1:3983753/1‑101 (MQ=18)
                              gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.cc                                                                        <  2:735686/101‑1 (MQ=255)
                               ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATAT.CCCAGGGGTGAAtt                                                            >  2:4111636/5‑101 (MQ=2)
                               ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.ccc                                                                       <  1:2231118/101‑1 (MQ=255)
                                ncNNGNNGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.cccn                                                                      <  1:2310865/100‑2 (MQ=25)
                                 gcggggggCTCTGCCCCCCGG.CTTC..TCTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATAT.CCCAGGGGTGAAttgg                                                          <  1:2690237/99‑3 (MQ=2)
                                 cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCgg                                                                     <  2:2820545/101‑1 (MQ=255)
                                  cgggggaCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G.....GGGCATGATTTCATCTTGCCCGAAATAT.CCCAGGGGTGAAttggc                                                         <  1:923282/100‑4 (MQ=2)
                                    gggggCGCTCCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCggggg                                                                  <  2:1586684/101‑1 (MQ=255)
                                      gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAAGCG.ATCCGGGCTTCGTTTCATCTTGCCCCCAATAT.CCCcgggggg                                                                >  1:1046620/1‑99 (MQ=21)
                                       ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCCAGATGAAAGCCG.ATCCGGGCTNCGNTNCNTCTTGCCCCCANTAT.CCCGGGGGGGa                                                               >  1:4984814/1‑101 (MQ=21)
                                          gcTCCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAAtt                                                            <  2:151496/101‑1 (MQ=255)
                                            tGCCCCCCGTCCTGCGGACTCCCCCCGGGGTATTTTAGGGAAAATGAAAACCG.GACCCGGGTTCGGTTTCTCTTTCCCCCCATATCCCCGGGGGGGAAttt                                                           >  1:45685/1‑101 (MQ=2)
                                            tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGACG.CTCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCggggggcaataga                                                          >  1:601237/1‑93 (MQ=21)
                                            tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAAtttt                                                          <  1:5046345/101‑1 (MQ=255)
                                            tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAAtttt                                                          >  2:2471759/1‑101 (MQ=255)
                                            tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAAtttt                                                          <  2:5474506/101‑1 (MQ=255)
                                            tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAAtttt                                                          <  2:1723904/101‑1 (MQ=255)
                                             gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTg                                                         <  1:4698144/101‑1 (MQ=255)
                                             gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTg                                                         <  2:177168/101‑1 (MQ=255)
                                              ccccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTgg                                                        <  1:3177913/101‑1 (MQ=255)
                                              ccccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTgg                                                        <  1:3696895/101‑1 (MQ=255)
                                                ccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGgcg                                                      <  2:1991723/101‑1 (MQ=255)
                                                ccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGgcg                                                      <  2:5673966/101‑1 (MQ=255)
                                                 cccGTCCTGCGGACTCCCCCCGGGGAATTTTAGGGAAAAAGAAAACCGAACCCGGGCTTCGTTTCTTTTTCCCCCAAAATC.CCCGGGGGGGATttttttgc                                                      >  1:16629/1‑97 (MQ=255)
                                                   cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAg                                                   <  1:1249312/97‑1 (MQ=255)
                                                   cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAg                                                   >  1:3345161/5‑101 (MQ=255)
                                                    gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGc                                                  <  2:692784/98‑1 (MQ=255)
                                                      ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCa                                                >  2:4313518/2‑101 (MQ=255)
                                                       cTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCaa                                               <  2:4873870/101‑1 (MQ=255)
                                                        tGCGGCCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCaaa                                              <  2:3983509/101‑1 (MQ=255)
                                                          cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAag                                            <  2:3530530/101‑1 (MQ=255)
                                                          cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGGGAATTTTGGCGCAGCCAAAag                                            >  1:5604183/1‑101 (MQ=255)
                                                             aCTCCCCCCGGGATATTTTAGGCAAGATGAAAAGCG.AACCGGGGTTCGTTTTATCTTGCCCCCAAAAT.TCCGGGGGGGAATTTTTGCGCCGCCCAAAGAgg                                         >  2:1077342/1‑101 (MQ=16)
                                                             aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                         <  1:1342282/101‑1 (MQ=255)
                                                             aCTCCCCCCGGGATATTTCAGGCAAGATGAAAGGCG.ATCCGGGCTTGGTTTCATCCTGCCCCAAATAT.CCCGGGGGGGAATTTTGGCGCAGCCAAAAGAgg                                         >  2:5183687/1‑101 (MQ=18)
                                                               tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                       >  1:557325/1‑101 (MQ=255)
                                                                  ccccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                    <  2:3313220/101‑1 (MQ=255)
                                                                  ccccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                    >  2:2404996/1‑101 (MQ=255)
                                                                   cccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                   <  2:5216750/101‑1 (MQ=255)
                                                                   cccGGGATATCTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                   <  2:120476/101‑1 (MQ=255)
                                                                    ccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                  <  2:213266/101‑1 (MQ=255)
                                                                    ccGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                  <  2:563656/101‑1 (MQ=255)
                                                                       gtatatTTGAGGCAACAGCAAAGGCG.ATCCGGGCCTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                               <  1:2509068/99‑1 (MQ=21)
                                                                       ggATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                               <  1:3037461/101‑1 (MQ=255)
                                                                        gATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                              <  1:1827960/101‑1 (MQ=255)
                                                                         atatTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                             >  1:669364/1‑101 (MQ=255)
                                                                         atatTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                             >  1:2048893/1‑101 (MQ=255)
                                                                          tatTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                            <  2:4556665/101‑1 (MQ=255)
                                                                           atTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                           >  1:447670/1‑101 (MQ=255)
                                                                           atTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                           >  1:2022021/1‑101 (MQ=255)
                                                                            tttGAGGCAAGATGCAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                          <  1:1591690/101‑1 (MQ=255)
                                                                             ttGAGGCAAGATGAAAGCCG.GTCCGGGCTTCGTTTCATCTTGCCCCAAATTT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                         <  1:2412119/101‑1 (MQ=255)
                                                                             tggAGGAAAGATGAAGGCCG.ATCCGGGCTGCGTTTCATCTTGCGCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCTCCCCCTAt                         <  2:159711/99‑1 (MQ=21)
                                                                              tGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                        >  1:4325051/1‑101 (MQ=255)
                                                                               gAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcc                       >  1:538964/1‑101 (MQ=255)
                                                                                 ggCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                     >  1:3183576/1‑101 (MQ=255)
                                                                                  gCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                    <  1:1560460/101‑1 (MQ=255)
                                                                                  gCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                    >  2:4994585/1‑101 (MQ=255)
                                                                                    aaGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                  >  1:842958/1‑101 (MQ=255)
                                                                                     aGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg                 >  2:2697940/1‑101 (MQ=255)
                                                                                       aTGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAACGAGGGGGCACCGCCCCCTATCCCcccgccg               >  2:1887502/1‑101 (MQ=25)
                                                                                         gAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg             >  2:2991101/1‑101 (MQ=255)
                                                                                           aaGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggccgcgc           >  1:1027197/1‑96 (MQ=255)
                                                                                           aaGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc           >  1:5553717/1‑101 (MQ=255)
                                                                                            aGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg          >  2:4679193/1‑101 (MQ=255)
                                                                                            aGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg          <  2:3137237/101‑1 (MQ=255)
                                                                                               cG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcg       >  2:3427831/1‑101 (MQ=255)
                                                                                                g.ATCCGGGCTTCGTTTCATCTTGCCCCAAATGT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc      >  1:5125998/1‑101 (MQ=255)
                                                                                                g.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc      >  1:4496320/1‑101 (MQ=255)
                                                                                                  aTCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg     >  2:4028998/1‑101 (MQ=255)
                                                                                                  aTCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg     >  2:3605901/1‑101 (MQ=255)
                                                                                                     cGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGgcgcggcggcgcggcg  >  1:4175124/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
TTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCG.ATCCGGGCTTCGTTTCATCTTGCCCCAAATAT.CCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCG  >  NC_014034/432372‑432571

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG