Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 735,234 Δ1 bp 2.7% intergenic (‑140/‑345) bchF ← / → ppaA 2‑vinyl bacteriochlorophyllide hydratase/regulatory protein PpaA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034735,2340C→. 2.7% 13.1 ‑0.3 ‑0.8261intergenic (‑140/‑345)bchF/ppaA2‑vinyl bacteriochlorophyllide hydratase/regulatory protein PpaA
Reads supporting (aligned to +/- strand):  new base (3/4):  ref base (129/125):  total (132/129)
Fisher's exact test strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.44e-01

Only 100 of 308 total aligned reads displayed.

AAAACTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCG  >  NC_014034/735134‑735333
                                                                                                     |                                                                                                   
aaaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.c                                                                                                     >  1:3687988/1‑101 (MQ=255)
aaaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.c                                                                                                     <  2:5252357/101‑1 (MQ=255)
aaaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.c                                                                                                     <  2:3381238/101‑1 (MQ=255)
 aaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.cc                                                                                                    >  1:265827/1‑101 (MQ=255)
   aCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.cccc                                                                                                  >  1:3497602/1‑101 (MQ=255)
    cTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.ccccc                                                                                                 <  1:1926792/101‑1 (MQ=255)
    cTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.ccccc                                                                                                 >  2:2088908/1‑101 (MQ=255)
    cTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.ccccc                                                                                                 >  2:5097045/1‑101 (MQ=255)
    cTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.ccccc                                                                                                 <  1:3548846/101‑1 (MQ=255)
      ggTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.ccccccc                                                                                               >  2:3709373/1‑101 (MQ=255)
        tCTTGTGACGTGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCCTGCGCGCAAGGCCGCCTTGATGCAGCGG.ccccccccc                                                                                             >  2:173294/1‑101 (MQ=255)
          ttGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCgg                                                                                           >  1:945986/1‑101 (MQ=255)
           tGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGc                                                                                          >  2:122877/1‑101 (MQ=255)
            gTCACGAGAAAGTGTCAATGAAAACTTACCCTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCt                                                                                         <  2:4248069/101‑1 (MQ=255)
             tCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTa                                                                                        >  2:679143/1‑101 (MQ=255)
             tCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTa                                                                                        <  2:1709094/101‑1 (MQ=255)
               aCGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTaaa                                                                                      <  2:2590761/101‑1 (MQ=255)
                cGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAAc                                                                                     <  2:3011136/101‑1 (MQ=255)
                 gagaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAAcc                                                                                    >  1:2281729/1‑101 (MQ=255)
                 gagaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAAcc                                                                                    <  1:4659772/101‑1 (MQ=255)
                  agaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACcg                                                                                   >  1:2964395/1‑101 (MQ=255)
                  agaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACcg                                                                                   <  2:4445859/101‑1 (MQ=255)
                  agaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACcg                                                                                   >  1:4484859/1‑101 (MQ=255)
                   gaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACCCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACcgc                                                                                  >  1:4779277/1‑101 (MQ=255)
                      aGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGt                                                                               <  2:3797398/101‑1 (MQ=255)
                         gtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGGCAc                                                                            >  2:2541191/1‑101 (MQ=255)
                          tCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACg                                                                           >  1:3000288/1‑101 (MQ=255)
                           cAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGa                                                                          <  2:3497385/101‑1 (MQ=255)
                           cAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGa                                                                          >  1:5647437/1‑101 (MQ=255)
                           cAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGa                                                                          >  1:111575/1‑101 (MQ=255)
                            aaTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGAc                                                                         <  1:173314/101‑1 (MQ=255)
                            aaTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGAc                                                                         >  1:3804824/1‑101 (MQ=255)
                            aaTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGAc                                                                         >  1:3595151/1‑101 (MQ=255)
                             aTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACt                                                                        >  1:4372889/1‑101 (MQ=255)
                             aTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACt                                                                        <  2:272116/101‑1 (MQ=255)
                               gAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCg                                                                      >  1:2373237/1‑101 (MQ=255)
                               gAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCAGGCTAAAACGCCGCCACCACGCg                                                                      >  2:789789/1‑101 (MQ=255)
                                  aaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTcc                                                                   >  2:3261109/1‑101 (MQ=255)
                                   tcTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCg                                                                  <  1:5514178/100‑1 (MQ=255)
                                   aCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCg                                                                  <  1:3199198/101‑1 (MQ=255)
                                   aCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGGCCg                                                                  >  1:1518163/1‑101 (MQ=255)
                                    cTTACACTCAGGGGCGGGACGAAACGCCGCTTCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCgg                                                                 <  1:4189640/101‑1 (MQ=255)
                                    cTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCCCCCgg                                                                 >  1:3934750/1‑101 (MQ=255)
                                      tACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCgaga                                                               >  2:5513886/1‑98 (MQ=255)
                                        cacTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAg                                                             <  1:5100608/101‑1 (MQ=255)
                                          ctgaGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCCAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaa                                                           <  2:1428053/98‑1 (MQ=255)
                                          cTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaa                                                           <  2:1538583/101‑1 (MQ=255)
                                          cTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaa                                                           <  1:5425245/101‑1 (MQ=255)
                                           tCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaaa                                                          <  1:408085/101‑1 (MQ=255)
                                           tCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaaa                                                          >  2:5368681/1‑101 (MQ=255)
                                           tCAGGGGCGGGACCAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCAGGGCAGaaa                                                          <  1:3868604/101‑1 (MQ=255)
                                            cAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAc                                                         >  2:4702495/1‑101 (MQ=255)
                                            cAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCCCGGCCACGCCTCGGCCGGGGGGAAAc                                                         >  1:3812352/1‑101 (MQ=255)
                                             aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAcc                                                        <  2:4630041/101‑1 (MQ=255)
                                             aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAcc                                                        <  2:2413365/101‑1 (MQ=255)
                                             aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCCGGCAGaaaac                                                        >  1:4914341/1‑99 (MQ=255)
                                             aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGGCCGGGCAGaaaac                                                        >  2:1339895/1‑99 (MQ=255)
                                              ggggCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGCCCGGGCAAAAAAcg                                                       >  2:2339161/1‑101 (MQ=255)
                                               gggCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACTACTCGTCCGGGCAGAAACcgg                                                      <  1:3276448/101‑1 (MQ=255)
                                               gggCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgg                                                      >  1:5034781/1‑101 (MQ=255)
                                                 gCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggc                                                    <  2:1295803/101‑1 (MQ=255)
                                                  cGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcg                                                   <  2:2027517/101‑1 (MQ=255)
                                                    ggACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcggg                                                 >  1:453708/1‑101 (MQ=255)
                                                      aCGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCCGGTTAACCCCCGGTAACGACCCGCCCGGCCGGAACCCggcggggg                                                >  1:1006037/1‑101 (MQ=255)
                                                        gAACCGCCGCTGCCCTTGCGCGGAAGGGCGCCTTGATGCAGGGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGggcggggcgg                                             <  2:487737/101‑1 (MQ=255)
                                                        gAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGggcggggcgg                                             >  2:4102531/1‑101 (MQ=255)
                                                        gAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGggcggggcgg                                             >  2:4066399/1‑101 (MQ=255)
                                                        gAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG..CCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGgcggggcggg                                            >  1:1476253/1‑101 (MQ=255)
                                                         aaaCGCCGCGGCCCTGGCGCGCAAGGGCGCNTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGgcggggcggg                                            <  1:863505/101‑1 (MQ=255)
                                                          aaCGCCTCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGa                                           <  2:1055340/101‑1 (MQ=255)
                                                          aaCGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGgcggggcgggg                                           >  1:5329301/1‑100 (MQ=255)
                                                           aCGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCCCGACTCGTCCGGGCAGAAACCGGgcggggcggggg                                          >  1:1808003/1‑99 (MQ=255)
                                                            cgccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATg                                         <  2:4412927/101‑1 (MQ=255)
                                                               cgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGgcggggcggggggggc                                      >  2:3121882/1‑95 (MQ=255)
                                                                gcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGcc                                     <  1:5348258/101‑1 (MQ=255)
                                                                 cTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAAACGCGGTCACGAATCGTCCGGGCAGAAACCGGGAGGGGGGGGAGGGGCCt                                    >  2:2946443/1‑101 (MQ=255)
                                                                     ccTTGGGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAGCCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtc                                <  2:888610/101‑1 (MQ=255)
                                                                     ccTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtc                                <  1:1431294/101‑1 (MQ=255)
                                                                      cTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGGGCCTTTtct                               >  1:1315977/1‑101 (MQ=255)
                                                                       ttGCGGGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtctc                              <  2:5647145/101‑1 (MQ=255)
                                                                       ttGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtctc                              <  2:5451423/101‑1 (MQ=255)
                                                                          cgcgcAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAAc                           <  1:736738/101‑1 (MQ=255)
                                                                           gcgcAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGGGCCTTTTCTCAAcc                          >  2:5692273/1‑101 (MQ=255)
                                                                           gcgcAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAAcc                          <  2:400578/101‑1 (MQ=255)
                                                                             gcAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTg                        <  2:3602980/101‑1 (MQ=255)
                                                                             ccAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTg                        <  2:5760703/100‑1 (MQ=255)
                                                                               aaGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGc                      >  2:2195158/1‑101 (MQ=255)
                                                                               aaGGGCGCCTTGATGCAGCGG..CCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGcc                     <  2:1875051/101‑1 (MQ=255)
                                                                                 gggCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGccc                    <  1:1707869/101‑1 (MQ=255)
                                                                                   gcgcCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGt                  >  2:4206025/1‑101 (MQ=255)
                                                                                    cgcCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTa                 <  1:3615137/101‑1 (MQ=255)
                                                                                     gcCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTAt                <  1:2815973/101‑1 (MQ=255)
                                                                                     gcCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTAt                <  2:245169/101‑1 (MQ=255)
                                                                                        ttGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCtt             <  1:127585/101‑1 (MQ=255)
                                                                                        ttGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCtt             <  1:1219674/101‑1 (MQ=255)
                                                                                            tGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgt         <  2:383555/101‑1 (MQ=255)
                                                                                              cAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtcg       <  1:2541333/101‑1 (MQ=255)
                                                                                                gCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTg     <  1:2013563/101‑1 (MQ=255)
                                                                                                  gg.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTggg   >  2:200292/1‑100 (MQ=255)
                                                                                                   g.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGCAACTTGCGTCGTGGCg  >  2:777194/1‑101 (MQ=255)
                                                                                                     |                                                                                                   
AAAACTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCG  >  NC_014034/735134‑735333

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG