BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	826,038	Δ1 bp	2.6%	coding (410/672 nt)	thiN →	thiamine pyrophosphokinase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	826,038	0	C→.	2.6%	7.0 ‑0.9 ‑0.0	191	coding (410/672 nt)	thiN	thiamine pyrophosphokinase
Reads supporting (aligned to +/- strand): new base (2/3): ref base (112/74): total (114/77)
Fisher's exact test strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.65e-01

Only 100 of 248 total aligned reads displayed.


GGGCTGCGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTGCGCTGGCCGATC  >  NC_014034/825938‑826138


  gCTGCGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGccc                                                                                                        <  2:2398166/101‑1 (MQ=255)


    tGCGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGccccc                                                                                                      <  1:3033498/101‑1 (MQ=255)


     gCGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGcccccc                                                                                                     <  2:4899472/101‑1 (MQ=255)


      cGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGccccccc                                                                                                    <  2:824282/101‑1 (MQ=255)


      cGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGccccccc                                                                                                    >  1:2401658/1‑101 (MQ=255)


       ggCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGcccccccc                                                                                                   <  1:3374378/101‑1 (MQ=255)


       ggCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGcccccccc                                                                                                   >  1:3394072/1‑101 (MQ=255)


       ggCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGcccccccc                                                                                                   <  1:2263912/101‑1 (MQ=255)


         gttGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCg                                                                                                 <  2:4256857/100‑1 (MQ=255)


          ttGAGCAAACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCAGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTAATCTTTCGCGCCCCCCCCCgg                                                                                                <  2:2865346/101‑1 (MQ=255)


           tGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGGTCCTGAACGCCGCGGACGAGGAGATCTTCCGCGGCCCCACCCgtc                                                                                               >  2:2032888/1‑99 (MQ=255)


           tGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGCGATCTTTCGCGCCCCCCCCCGGc                                                                                               >  1:929029/1‑101 (MQ=255)


             aTCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTg                                                                                             <  1:3562556/101‑1 (MQ=255)


               cacaCGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGccccccccggccggcc                                                                                           >  1:1967455/1‑93 (MQ=255)


               cacaCGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCCGgc                                                                                           >  1:5490704/1‑101 (MQ=255)


               cacaCGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGGGATCTTTCGCGCCCCCCCCCGGCTGgc                                                                                           >  1:5264010/1‑101 (MQ=255)


                acacGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGgcg                                                                                          >  1:3316901/1‑101 (MQ=255)


                acacGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGgcg                                                                                          >  2:747529/1‑101 (MQ=255)


                    gCTGGCGGCGCTGACCGAACGGCCCCCGCGGCCCGGGCAGACCGTGTCCCTAATCGCGGGGGACGGGTTATTTTTTCGCGCCCCCCCCCGGCTGGCGCTgg                                                                                      <  2:1061408/101‑1 (MQ=255)


                     cTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGa                                                                                     <  1:4911076/101‑1 (MQ=255)


                     cTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGAT.CTGATCGCCGAGGACGAGGGGAACTTTCGCGCCCCCCCCCCGGCGGCGCttggt                                                                                    >  2:1946216/1‑97 (MQ=255)


                         cggcgCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCGGGCGCTGGGTCTg                                                                                 >  1:4428018/1‑101 (MQ=255)


                           gcgcTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGaccggct                                                                               <  2:5501399/101‑7 (MQ=255)


                            cgcTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGccc                                                                              <  2:2618489/101‑1 (MQ=255)


                               tGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTg                                                                           <  1:2879596/101‑1 (MQ=255)


                               tGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTg                                                                           <  1:1397915/101‑1 (MQ=255)


                                gactcaaCTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTGGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTgg                                                                          >  2:2173342/6‑101 (MQ=255)


                                gACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTgg                                                                          >  1:396444/1‑101 (MQ=255)


                                 aCCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCGGGATCTGCCCTTggg                                                                         >  2:741687/1‑101 (MQ=255)


                                    gAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCAc                                                                      <  2:35606/101‑1 (MQ=255)


                                     aaCTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCAcg                                                                     <  2:1152238/101‑1 (MQ=255)


                                     aaCTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCAcg                                                                     <  2:1395206/101‑1 (MQ=255)


                                      aCTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGGGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCAcgc                                                                    >  1:1641647/1‑101 (MQ=255)


                                      aCTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCCGCTGGCGCGGGATCTGCCCTTGGGCAagc                                                                    >  2:3748548/1‑101 (MQ=255)


                                           ccACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTg                                                               >  1:448834/1‑101 (MQ=255)


                                            cACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGt                                                              >  2:4647974/1‑101 (MQ=255)


                                             aCGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCCCGCGGCTgcc                                                             >  1:1721083/1‑99 (MQ=255)


                                             aCGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTc                                                             >  2:4394650/1‑101 (MQ=255)


                                             aCGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTc                                                             <  1:144989/101‑1 (MQ=255)


                                                cgGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCt                                                          <  1:3484691/101‑1 (MQ=255)


                                                cgGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCt                                                          <  2:5776/101‑1 (MQ=255)


                                                 ggCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTg                                                         >  1:4807356/1‑101 (MQ=255)


                                                 ggCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGCGATCTTTCGCGCCCCCCCCCGGCTGGCGCCGGATCTGCCCTTGGGCCCGCGGCTGTCGCTg                                                         >  2:902588/1‑101 (MQ=255)


                                                   cccGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCa                                                       <  1:1154553/101‑1 (MQ=255)


                                                    ccGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCAt                                                      >  2:2939437/1‑101 (MQ=255)


                                                     cGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGAATc                                                     >  2:3346948/1‑101 (MQ=255)


                                                      gggCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATcc                                                    <  1:743714/101‑1 (MQ=255)


                                                      gggCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATcc                                                    >  2:467332/1‑101 (MQ=255)


                                                      gggCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCG.CCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCg                                                   <  2:1967344/101‑1 (MQ=255)


                                                       ggCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCg                                                   <  2:5465073/101‑1 (MQ=255)


                                                        gCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGGACGCGGCTGTCGCTGCATCCG.a                                                 >  2:3684594/1‑101 (MQ=255)


                                                         cAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.At                                                >  1:774830/1‑101 (MQ=255)


                                                         cAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.At                                                >  1:3102398/1‑101 (MQ=255)


                                                         cAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.At                                                <  2:2219185/101‑1 (MQ=255)


                                                          aGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATg                                               >  2:4865879/1‑101 (MQ=255)


                                                          aGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATg                                               <  2:1549242/101‑1 (MQ=255)


                                                            aTCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATggg                                             <  2:5618075/101‑1 (MQ=255)


                                                             tCGTGATCCTGATCGCCGAGGACGAGGTTATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGa                                            <  1:2463121/101‑1 (MQ=255)


                                                             tCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGa                                            >  1:5733659/1‑101 (MQ=255)


                                                               gTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGAcc                                          <  1:3853076/101‑1 (MQ=255)


                                                               gTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGAcc                                          >  2:4289670/1‑101 (MQ=255)


                                                                tGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCg                                         <  1:2224738/101‑1 (MQ=255)


                                                                tGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGCCGCTTGATCCG.ATGGGACCg                                         >  2:3767606/1‑101 (MQ=255)


                                                                    ccTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCtt                                     >  1:4715825/1‑101 (MQ=255)


                                                                    ccTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCtt                                     <  1:1774951/101‑1 (MQ=255)


                                                                      tGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCtttc                                   >  2:3075591/1‑99 (MQ=255)


                                                                      tGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTcc                                   >  1:1219865/1‑101 (MQ=255)


                                                                       gATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCg                                  <  1:1178817/101‑1 (MQ=255)


                                                                        aTCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCGGGCGCTGGATCTGCCCTTGGGGACGCGGCTGTCGCTGCGTCCGCACGGAACCGCCTTCCg                                  >  2:4207257/1‑101 (MQ=255)


                                                                             cGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGggg.cc                           >  1:1322058/1‑100 (MQ=255)


                                                                             cGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.Ct                           <  2:1265994/101‑1 (MQ=255)


                                                                              gAGGACGAGGGGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGCTCGGGGCTTGGGCCGGCGGCTCTCGCTTCCTCCG.ATCGGGCCCGCTTCCGGGCT.CTc                          >  2:2252643/1‑101 (MQ=255)


                                                                               ggggcggggTGATCTTTCGCCCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCg                         <  2:4515982/94‑1 (MQ=255)


                                                                               aGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGCTCTGCCCTTGGGCCCGCGGCTGTCGCTCCAACCT.ATGGGACCGGCTTccccgcccccc                          >  2:4096155/1‑92 (MQ=255)


                                                                               aGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTcc                         >  1:4314435/1‑100 (MQ=255)


                                                                                ggACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGGACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.a                       <  1:3684822/101‑1 (MQ=255)


                                                                                  aCGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.Acc                     <  2:4306431/101‑1 (MQ=255)


                                                                                   cGAGGTGATCTTGCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.g                   <  2:2437715/101‑1 (MQ=255)


                                                                                       gTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCt               <  2:396400/101‑1 (MQ=255)


                                                                                       gTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCCGGCG.CTCG.GCC.ggcgt               >  1:1783808/1‑98 (MQ=255)


                                                                                          aTCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTgcg            <  1:2493192/101‑1 (MQ=255)


                                                                                          aTCTTTCGCG.CCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCC.GTCGCACC.gggggcgc            >  1:1020090/1‑96 (MQ=255)


                                                                                           tCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTgcgc           >  2:439625/1‑101 (MQ=255)


                                                                                           tCTTTCGCCCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTgcgc           <  2:1090252/101‑1 (MQ=255)


                                                                                            tttccgccCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTGCGCt          <  2:5161481/97‑1 (MQ=255)


                                                                                            ccttggcgcCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGGCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTGCGCt          <  1:902664/96‑1 (MQ=255)


                                                                                             tttCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTGCGCTg         <  1:457237/101‑1 (MQ=255)


                                                                                             tttCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.CGGCTgcgccg         >  2:1823222/1‑99 (MQ=255)


                                                                                              ttCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGTCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTGCGCTgg        <  2:4563320/101‑1 (MQ=255)


                                                                                              ttCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGGG.CTCG.ACC.GGGCTggggcgg        >  2:3948843/1‑95 (MQ=255)


                                                                                               tCGCGGCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTGCGCTGGc       <  2:1720984/101‑1 (MQ=255)


                                                                                                cgcgCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGAGCGGCTTCCGGGCG.CTCG.GCC.GGGGCGCGCTgggc      >  2:3243503/1‑99 (MQ=255)


                                                                                                 gcgcCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTGCGCTGGCCg     <  2:1485173/101‑1 (MQ=255)


                                                                                                 gcgcCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGAACGGCTTTCGGGCGCCTGG.ACC..GGATGCGCTGGCCg     >  1:2144662/1‑101 (MQ=255)


                                                                                                   gcCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTTCCGGCG.CCCG.GCC.GGGGGGCGCCGGGCggt   >  2:5148394/1‑99 (MQ=255)


                                                                                                   gcCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGGG.CTCG.ACC.CGGCTGCGCTGGCCGAt   >  1:2329643/1‑101 (MQ=255)


                                                                                                   gcCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTGCGCCCGCCGAt   >  1:2676259/1‑101 (MQ=255)


                                                                                                    cccccccccGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTTCGGGCG.CTCG.CCCAGGGCT.CGCCGGcccagc  >  2:2475763/1‑97 (MQ=255)


                                                                                                    cccccccccGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTGCGCTGGCCGATc  <  1:1845510/101‑1 (MQ=255)


                                                                                                    cccccccccGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTGCGCTGGCCGATc  <  2:3322315/101‑1 (MQ=255)


GGGCTGCGGCTTGATCACACGCTGGCGGCGCTGACCGAACTGACCACGCGGCCCGGGCAGATCGTGATCCTGATCGCCGAGGACGAGGTGATCTTTCGCGCCCCCCCCCGGCTGGCGCTGGATCTGCCCTTGGGCACGCGGCTGTCGCTGCATCCG.ATGGGACCGGCTTCCGGGCG.CTCG.ACC.GGGCTGCGCTGGCCGATC  >  NC_014034/825938‑826138

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG