Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,065,845 +C 98.5% intergenic (‑17/+117) RCAP_rcc00969 ← / ← RCAP_rcc00970 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,065,8451.→C98.5% 3.0 ‑0.5 ‑0.6272intergenic (‑17/+117)RCAP_rcc00969/RCAP_rcc00970hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (128/140):  ref base (1/3):  total (129/143)
Fisher's exact test strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.34e-01

Only 100 of 311 total aligned reads displayed.

GTGCACCTCGGTCACCCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGG.CTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCAGCCGCGCGCAGATC  >  NC_014034/1065745‑1065942
                                                                                                       |                                                                                                 
gTGCACCTCGGTCACCCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.gg.ggg                                                                                                    >  1:3254113/1‑101 (MQ=255)
gTGCACCTCGGTCACCCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.gg.ggg                                                                                                    >  1:2769531/1‑101 (MQ=255)
 tGCACCTCGGTCACCCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGG.c                                                                                                  >  2:4664215/1‑101 (MQ=255)
   cccgtgggTCCCCCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGG.cct                                                                                                <  2:2577261/95‑3 (MQ=255)
    cccTGGGTCCGCCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGG.cctc                                                                                               <  1:5608677/100‑4 (MQ=255)
    cccTCGGTCACCCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGG.cctc                                                                                               <  2:2749281/100‑4 (MQ=255)
     ccTGGGCCACCCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGG.cctcg                                                                                              <  2:1936101/101‑5 (MQ=255)
       tCGGTCACCCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGAt                                                                                             >  2:1043450/1‑101 (MQ=255)
       tCGGTCAACCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGAt                                                                                             >  1:1883603/1‑101 (MQ=255)
       ccggccccccGCCACTGGGGCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGAt                                                                                             <  1:1269497/94‑1 (MQ=255)
       ccGGTCCCCCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGAt                                                                                             <  2:3824691/100‑1 (MQ=255)
         ggccccccGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GGTGGC.CTCGAtct                                                                                           <  2:3613265/96‑1 (MQ=255)
         ggTCACCCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGAtct                                                                                           >  2:3058986/1‑101 (MQ=255)
         ggTCACCCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGAtct                                                                                           >  2:5256762/1‑101 (MQ=255)
          gccccccGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGAtctc                                                                                          <  1:2616705/97‑1 (MQ=255)
                  ccACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAAATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCa                                                                                  <  2:4197069/101‑1 (MQ=255)
                   cACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCAt                                                                                 <  1:5373810/101‑1 (MQ=255)
                    aCTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATc                                                                                <  1:2037292/101‑1 (MQ=255)
                     cTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCg                                                                               >  2:1570279/1‑101 (MQ=255)
                      tGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCgc                                                                              >  2:542564/1‑101 (MQ=255)
                       gggTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCgca                                                                             <  2:4074240/101‑1 (MQ=255)
                       gggTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCgca                                                                             >  2:145228/1‑101 (MQ=255)
                        ggTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCgcag                                                                            <  1:4877796/101‑1 (MQ=255)
                        ggTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCgcag                                                                            <  1:924036/101‑1 (MQ=255)
                        ggTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCgcag                                                                            <  2:591611/101‑1 (MQ=255)
                          tCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCgcagca                                                                          <  2:1498335/101‑1 (MQ=255)
                            tGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCaaa                                                                        >  1:3015070/1‑101 (MQ=255)
                             ggATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAAc                                                                       <  1:3859813/101‑1 (MQ=255)
                              gATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACt                                                                      >  1:2238947/1‑101 (MQ=255)
                               aTGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTg                                                                     <  1:601288/101‑1 (MQ=255)
                               aTGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTg                                                                     >  1:1417020/1‑101 (MQ=255)
                                tGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTgc                                                                    >  1:5079487/1‑101 (MQ=255)
                                 gTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTgcg                                                                   <  1:885953/101‑1 (MQ=255)
                                  tCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTgcgc                                                                  >  2:4828007/1‑101 (MQ=255)
                                   cGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGcgcg                                                                 <  1:4394368/101‑1 (MQ=255)
                                    gAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCgcgg                                                                >  1:3820258/1‑101 (MQ=255)
                                    gAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCgcgg                                                                >  2:5611277/1‑101 (MQ=255)
                                      aaaaGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCgcggcg                                                              <  2:2390358/101‑1 (MQ=255)
                                        aaGGCCGCGGCAGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGAt                                                            <  2:1341558/101‑1 (MQ=255)
                                         aGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATg                                                           <  2:3547957/101‑1 (MQ=255)
                                         aGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATg                                                           >  2:1182431/1‑101 (MQ=255)
                                          ggCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGt                                                          <  1:3117020/101‑1 (MQ=255)
                                           gCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTc                                                         >  2:3672644/1‑101 (MQ=255)
                                           gCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTc                                                         <  2:4440185/101‑1 (MQ=255)
                                             cgcgACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCgg                                                       >  2:80507/1‑101 (MQ=255)
                                             cgcgACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCgg                                                       >  1:3351497/1‑101 (MQ=255)
                                              gcgACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCggg                                                      >  1:233033/1‑101 (MQ=255)
                                              gcgACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCggg                                                      >  2:3853887/1‑101 (MQ=255)
                                                gACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGgtg                                                    >  1:2368198/1‑101 (MQ=255)
                                                 aCCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGgtgt                                                   <  2:3764036/101‑1 (MQ=255)
                                                 aCCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCGCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGgtgt                                                   <  1:1043525/101‑1 (MQ=255)
                                                  ccGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGtt                                                  <  2:5029237/101‑1 (MQ=255)
                                                    gTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGc                                                >  2:277121/1‑101 (MQ=255)
                                                    gTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGc                                                >  1:2009753/1‑101 (MQ=255)
                                                     cccGCGCGTGGCACTGCCAGATCTCGATCACCTCCTGGGTGGC.GG.GGGCCTCGATCTCGCCCCCCATCGCAGCAAACTGCGCGGCGATGTGGGGTGTTGcc                                               <  1:145248/100‑1 (MQ=255)
                                                      ccGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCa                                              <  1:1784190/101‑1 (MQ=255)
                                                       cgcgcgTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCAc                                             <  1:4664491/101‑1 (MQ=255)
                                                           cgTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACa                                         <  1:3935901/101‑1 (MQ=255)
                                                           cgTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACa                                         >  2:5467980/1‑101 (MQ=255)
                                                            gTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAg                                        <  1:4515323/101‑1 (MQ=255)
                                                            gTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAg                                        >  2:4917568/1‑101 (MQ=255)
                                                            gTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAg                                        >  2:2009472/1‑101 (MQ=255)
                                                            gTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAg                                        >  1:2830136/1‑101 (MQ=255)
                                                             tGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAgg                                       <  1:5317587/101‑1 (MQ=255)
                                                              ggCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGt                                      <  1:5188369/101‑1 (MQ=255)
                                                               gCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTc                                     <  2:3177742/101‑1 (MQ=255)
                                                                cACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCg                                    >  1:2414419/1‑101 (MQ=255)
                                                                cACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCg                                    <  1:1169205/101‑1 (MQ=255)
                                                                cACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTAGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCg                                    <  1:2158559/101‑1 (MQ=255)
                                                                   tGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCt                                 <  2:2238821/101‑1 (MQ=255)
                                                                   tGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCt                                 <  1:2534137/101‑1 (MQ=255)
                                                                    gCCAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCtt                                <  2:897824/101‑1 (MQ=255)
                                                                     ccAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTg                               <  2:3253913/101‑1 (MQ=255)
                                                                     ccAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTg                               >  1:3644023/1‑101 (MQ=255)
                                                                      cAGATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTgg                              >  2:3956226/1‑101 (MQ=255)
                                                                       aGATCTCGATCACATCCTCGGTGACAGG.GGC.CTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGa                             >  2:501771/1‑101 (MQ=255)
                                                                        gATCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGAt                            <  2:2958420/101‑1 (MQ=255)
                                                                         aTCTCGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATc                           <  2:2045510/101‑1 (MQ=255)
                                                                           ctcGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGa                         >  1:4202713/1‑101 (MQ=255)
                                                                           ctcGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGa                         >  2:4318055/1‑101 (MQ=255)
                                                                             cGATCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGAcc                       >  1:3983052/1‑101 (MQ=255)
                                                                               aTCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCg                     <  1:1521207/101‑1 (MQ=255)
                                                                               aTCACATCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCg                     <  1:574289/101‑1 (MQ=255)
                                                                                 cacaTCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGc                   >  1:3232652/1‑101 (MQ=255)
                                                                                 cacaTCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGc                   >  2:5315329/1‑101 (MQ=255)
                                                                                  ccaTCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGcc                  <  2:1733014/100‑1 (MQ=255)
                                                                                    aTCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCTCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCa                <  1:4036437/101‑1 (MQ=255)
                                                                                    aTCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCa                <  1:3421182/101‑1 (MQ=255)
                                                                                    aTCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCa                >  1:1691094/1‑101 (MQ=255)
                                                                                     tCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCAg               <  1:1570373/101‑1 (MQ=255)
                                                                                     tCCTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCAg               <  2:3973710/101‑1 (MQ=255)
                                                                                      ccTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCAGc              <  2:3351292/101‑1 (MQ=255)
                                                                                      ccTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCAGc              <  1:504830/101‑1 (MQ=255)
                                                                                       cTCGGTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCAGcc             >  1:4776008/1‑101 (MQ=255)
                                                                                           gTGAC.GG.GGGCCTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCAGCcgcgc         <  2:1612069/101‑1 (MQ=255)
                                                                                            tgccggg.ggc.CTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCAGCcgcgcg        >  1:4274053/6‑101 (MQ=255)
                                                                                               cggg.ggc.CTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCAGCCGCGCGCAg     >  1:5456045/3‑101 (MQ=255)
                                                                                                ggg.ggc.CTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCAGCCGCGCGCAGa    >  2:2303361/2‑101 (MQ=255)
                                                                                                 gg.ggc.CTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCAGCCGCGCGCAGAt   >  2:5116715/1‑101 (MQ=255)
                                                                                                  g.ggc.CTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCAGCCGCGCGCAGATc  <  1:1496042/101‑1 (MQ=255)
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GTGCACCTCGGTCACCCGCCACTGGGTCTGGATGTCGAAAAAGGCCGCGACCGTCCGCGCGTGGCACTGCCAGATCTCGATCACATCCTCGGTGAC.GG.GGG.CTCGATCTCGGCCCCCATCGCAGCAAACTGCGCGGCGATGTCGGGTGTTGCCACGACAGGTCGGCTTGGATCGACCCGGCCCAGCCGCGCGCAGATC  >  NC_014034/1065745‑1065942

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG