Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,398,031 +C 1.5% intergenic (+335/+1563) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,398,0312.→C 1.5% 3.9 0.0 ‑0.6270intergenic (+335/+1563)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (130/136):  total (132/138)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.29e-01

Only 100 of 297 total aligned reads displayed.

AAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATT  >  NC_014034/1397931‑1398131
                                                                                                                                                                                                         
    tctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGcg                                                                                                  >  1:3980761/1‑100 (MQ=255)
    tctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCa                                                                                                  >  2:4736212/1‑101 (MQ=255)
     ctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCaa                                                                                                 >  2:1977353/1‑101 (MQ=255)
      tATGGGAGCCTAGGGAAGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAg                                                                                                <  2:4437200/101‑1 (MQ=255)
       aTGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGt                                                                                               <  1:1799828/101‑1 (MQ=255)
       aTGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGt                                                                                               <  2:5669236/101‑1 (MQ=255)
        tGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTc                                                                                              >  2:5587591/1‑101 (MQ=255)
        tGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTc                                                                                              <  1:3147309/101‑1 (MQ=255)
        tGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTc                                                                                              >  1:2029551/1‑101 (MQ=255)
         gggaggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCg                                                                                             >  1:3647061/1‑101 (MQ=255)
         gggaggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCg                                                                                             <  2:5559986/101‑1 (MQ=255)
             ggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTcc                                                                                         <  1:3890589/101‑1 (MQ=255)
              gctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTccc                                                                                        >  2:157567/1‑101 (MQ=255)
              gctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTccc                                                                                        <  2:1939131/101‑1 (MQ=255)
                 agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTcccccc                                                                                     <  2:402247/101‑1 (MQ=255)
                 agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTcccccc                                                                                     >  1:2310153/1‑101 (MQ=255)
                   ggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCcgc                                                                                   >  2:4655791/1‑101 (MQ=255)
                     gggctaCGCCCCTTCAAAAGTCGCTCCCCCCGCGGGGCCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCgg                                                                                 <  2:1367250/100‑1 (MQ=255)
                     aggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCgg                                                                                 <  1:2172995/101‑1 (MQ=255)
                      ggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCggg                                                                                <  1:1511433/101‑1 (MQ=255)
                        ctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCggggg                                                                              >  2:439287/1‑101 (MQ=255)
                         taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGc                                                                             >  1:5700832/1‑101 (MQ=255)
                         taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGc                                                                             <  1:1583655/101‑1 (MQ=255)
                         taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGc                                                                             >  1:1100677/1‑101 (MQ=255)
                            gccccTTCAAAAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCGCCGCGTAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCCGGGGAGTg                                                                          >  2:3477933/4‑101 (MQ=37)
                            gCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTg                                                                          <  1:4736493/101‑1 (MQ=255)
                             cACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  1:4677303/101‑1 (MQ=255)
                              cccTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                        <  2:5361984/100‑1 (MQ=255)
                               ccTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  1:4828444/101‑1 (MQ=255)
                               ccTTCAAAAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  1:4159940/101‑1 (MQ=255)
                                cTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  2:3429610/101‑1 (MQ=255)
                                cTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      >  2:3743021/1‑101 (MQ=255)
                                cTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  2:5650296/101‑1 (MQ=255)
                                 ttCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     >  2:2746258/1‑101 (MQ=255)
                                    aaaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTaa                                                                  <  2:5767564/101‑1 (MQ=255)
                                     aaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAg                                                                 <  1:4383458/101‑1 (MQ=255)
                                      aaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGc                                                                <  1:4656067/101‑1 (MQ=255)
                                      aaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGc                                                                >  2:3512476/1‑101 (MQ=255)
                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCggg                                                             >  2:1026179/1‑100 (MQ=255)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGATCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTg                                                            >  1:2579662/1‑101 (MQ=255)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGGGc                                                           >  1:2446368/1‑101 (MQ=255)
                                            cTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCa                                                          >  1:3250909/1‑101 (MQ=255)
                                             cccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCAt                                                         <  2:2114564/100‑1 (MQ=255)
                                             cccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCAt                                                         <  2:5493871/100‑1 (MQ=255)
                                               cccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCg                                                       <  1:5557874/101‑1 (MQ=255)
                                                ccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCgg                                                      >  1:2646138/1‑101 (MQ=255)
                                                 cccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGc                                                     >  2:5083289/1‑101 (MQ=255)
                                                  ccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGcc                                                    >  2:2147088/1‑101 (MQ=255)
                                                   cgcgGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGccc                                                   <  1:5136228/101‑1 (MQ=255)
                                                    gcgGGGGCGTGGATCGAACCCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCg                                                  <  1:1024253/101‑1 (MQ=255)
                                                    gcgGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCg                                                  >  1:1745807/1‑101 (MQ=255)
                                                      gggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGAt                                                >  1:76050/1‑101 (MQ=255)
                                                      gggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGAt                                                <  1:5403303/101‑1 (MQ=255)
                                                      gggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGAt                                                <  1:5173747/101‑1 (MQ=255)
                                                        gggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGGGGAGCAAGTCGCTCCCCCCGCGGGGGCGAGGATCGTAAGCGGTGCCTCGGCCCGAGaa                                              >  2:3059849/1‑101 (MQ=39)
                                                        gggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATaa                                              >  2:482868/1‑101 (MQ=255)
                                                          gCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAc                                            <  1:5297224/101‑1 (MQ=255)
                                                           cGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAcc                                           <  2:5256289/101‑1 (MQ=255)
                                                             tGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAcccc                                         <  2:599890/101‑1 (MQ=255)
                                                              ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg                                        <  2:2972194/101‑1 (MQ=255)
                                                              ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg                                        <  1:1815706/101‑1 (MQ=255)
                                                              ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGGGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg                                        >  2:1845163/1‑101 (MQ=255)
                                                               gATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGa                                       >  2:1274834/1‑101 (MQ=255)
                                                                  cGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAttt                                    <  2:116135/101‑1 (MQ=255)
                                                                   gAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAtttt                                   <  2:221605/101‑1 (MQ=255)
                                                                   gAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAtttt                                   >  1:881922/1‑101 (MQ=255)
                                                                    aaaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGCTTTTc                                  >  2:3320016/1‑101 (MQ=255)
                                                                     aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAATTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAttttag                                 <  2:2962563/101‑3 (MQ=255)
                                                                     aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg                                 >  1:698187/1‑101 (MQ=255)
                                                                     aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg                                 <  2:4691261/101‑1 (MQ=255)
                                                                       ccgccgCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGc                               >  1:2115626/1‑101 (MQ=255)
                                                                            gcgcAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTg                          <  2:854930/101‑1 (MQ=255)
                                                                            gcgcAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTg                          <  2:3967236/101‑1 (MQ=255)
                                                                            gcgcAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTg                          <  2:3577853/101‑1 (MQ=255)
                                                                               cAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgg                       <  2:4156830/101‑1 (MQ=255)
                                                                               cAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgg                       <  1:5505232/101‑1 (MQ=255)
                                                                               cAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgg                       >  2:5186830/1‑101 (MQ=255)
                                                                                caaaaGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAggg                      <  2:2560171/100‑1 (MQ=255)
                                                                                aaaaaGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAATCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAggg                      >  1:4308650/1‑101 (MQ=255)
                                                                                aaaaaGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGCggg                      >  2:2585579/1‑101 (MQ=255)
                                                                                aaaaaGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAggg                      <  1:1046921/101‑1 (MQ=255)
                                                                                  aaaGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGa                    >  1:1199097/1‑101 (MQ=255)
                                                                                     gCCCGCCAAGAAGCGGAGCGAGTCGCCCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAg                 <  1:3478150/101‑1 (MQ=255)
                                                                                      cccGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGa                <  2:2463380/101‑1 (MQ=255)
                                                                                      cccGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGa                <  1:2921120/101‑1 (MQ=255)
                                                                                        cGCCAAGAAGCGGAGCAAGTCGCCCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGAtt              <  1:796389/101‑1 (MQ=255)
                                                                                         gCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTc             <  1:2456549/101‑1 (MQ=255)
                                                                                          ccAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCt            >  1:3010605/1‑101 (MQ=255)
                                                                                          ccAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCt            <  2:1462561/101‑1 (MQ=255)
                                                                                           cAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCtt           <  2:3250709/101‑1 (MQ=255)
                                                                                           cAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCtt           <  1:1129853/101‑1 (MQ=255)
                                                                                             agaagCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTgg         <  1:1274917/101‑1 (MQ=255)
                                                                                             agaagCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTgg         <  1:3840792/101‑1 (MQ=255)
                                                                                               aagCGGAGCCAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGca       <  2:4286140/101‑1 (MQ=255)
                                                                                                agCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcac      <  1:279655/101‑1 (MQ=255)
                                                                                                agCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcac      >  2:3940353/1‑101 (MQ=255)
                                                                                                  cGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACaa    <  1:3934621/101‑1 (MQ=255)
                                                                                                   ggAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATGGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAt   <  1:4962230/101‑1 (MQ=255)
                                                                                                   ggAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAt   >  1:5601843/1‑101 (MQ=255)
                                                                                                   ggAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAt   <  2:790027/101‑1 (MQ=255)
                                                                                                                                                                                                         
AAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATT  >  NC_014034/1397931‑1398131

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG