Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,398,035 +C 1.5% intergenic (+339/+1559) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,398,0351.→C 1.5% 3.9 0.0 ‑0.6271intergenic (+339/+1559)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (126/141):  total (128/143)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.62e-01

Only 100 of 293 total aligned reads displayed.

TCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATTCCGC  >  NC_014034/1397935‑1398135
                                                                                                       |                                                                                                    
tctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCa                                                                                                       >  2:2542963/1‑101 (MQ=255)
tctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCa                                                                                                       >  1:4329449/1‑101 (MQ=255)
tctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCa                                                                                                       >  2:4736212/1‑101 (MQ=255)
 ctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCa.a                                                                                                     <  2:3772136/101‑1 (MQ=255)
 ctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCa.a                                                                                                     >  2:1977353/1‑101 (MQ=255)
  tATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.Ag                                                                                                    <  2:2691083/101‑1 (MQ=255)
  tATGGGAGCCTAGGGAAGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.Ag                                                                                                    <  2:4437200/101‑1 (MQ=255)
   aTGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGt                                                                                                   >  1:854999/1‑101 (MQ=255)
   aTGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGt                                                                                                   <  2:5669236/101‑1 (MQ=255)
    tGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTc                                                                                                  >  1:5667980/1‑101 (MQ=255)
    tGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTc                                                                                                  >  1:2029551/1‑101 (MQ=255)
    tGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTc                                                                                                  >  2:5587591/1‑101 (MQ=255)
     gggaggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCg                                                                                                 <  2:5559986/101‑1 (MQ=255)
         ggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcc                                                                                             <  1:3890589/101‑1 (MQ=255)
          gctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTccc                                                                                            <  2:1939131/101‑1 (MQ=255)
             agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcccccc                                                                                         >  1:2310153/1‑101 (MQ=255)
             agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcccccc                                                                                         <  2:402247/101‑1 (MQ=255)
               ggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCcgc                                                                                       >  2:4655791/1‑101 (MQ=255)
                gaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCcgcg                                                                                      <  2:343126/101‑1 (MQ=255)
                 aggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCgg                                                                                     <  1:2172995/101‑1 (MQ=255)
                   gctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCgggg                                                                                   >  2:51726/1‑101 (MQ=255)
                    ctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCggggg                                                                                  >  2:439287/1‑101 (MQ=255)
                     taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGc                                                                                 <  1:1583655/101‑1 (MQ=255)
                     taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGc                                                                                 >  1:751215/1‑101 (MQ=255)
                     taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGc                                                                                 >  1:1100677/1‑101 (MQ=255)
                        gCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTg                                                                              <  1:4736493/101‑1 (MQ=255)
                         cACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTgg                                                                             <  1:4677303/101‑1 (MQ=255)
                          cccTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGa                                                                            <  2:5361984/100‑1 (MQ=255)
                           ccTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                           <  1:4828444/101‑1 (MQ=255)
                            cTTCAAATGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTTGATc                                                                          <  2:3734870/101‑1 (MQ=38)
                            cTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                          <  2:5650296/101‑1 (MQ=255)
                              tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.CGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                          <  1:2512478/95‑1 (MQ=2)
                               cAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTa                                                                       <  2:5221852/101‑1 (MQ=255)
                               cAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGGGGATCGTa                                                                       >  1:2599025/1‑101 (MQ=255)
                                aaaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTaa                                                                      <  2:5767564/101‑1 (MQ=255)
                                 aaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAg                                                                     >  1:785133/1‑101 (MQ=255)
                                   aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGTGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCg                                                                   >  1:5597775/1‑101 (MQ=255)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGGGc                                                               >  1:2446368/1‑101 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCa                                                              >  2:2920953/1‑101 (MQ=255)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCAt                                                             >  1:3967480/1‑101 (MQ=255)
                                         cccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCAt                                                             <  2:5493871/100‑1 (MQ=255)
                                         cccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCAt                                                             <  2:2114564/100‑1 (MQ=255)
                                          ccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATc                                                            >  2:2456415/1‑101 (MQ=255)
                                           cccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCg                                                           <  1:5557874/101‑1 (MQ=255)
                                              ccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGcc                                                        >  2:2147088/1‑101 (MQ=255)
                                                gcgGGGGCGTGGATCGAACCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCg                                                      <  1:1024253/101‑1 (MQ=255)
                                                gcgGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCg                                                      >  1:1745807/1‑101 (MQ=255)
                                                  gggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGAt                                                    >  2:3511591/1‑101 (MQ=255)
                                                   ggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATa                                                   >  2:1772302/1‑101 (MQ=255)
                                                   ctggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATa                                                   <  1:1240763/99‑1 (MQ=255)
                                                    gggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGGGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGAGGATCGTAAGCGGTGCCTCGGCCCGAGaa                                                  >  2:3059849/1‑101 (MQ=39)
                                                    gggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATaa                                                  <  1:4423123/101‑1 (MQ=255)
                                                     ggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATaag                                                 >  2:1240680/1‑100 (MQ=255)
                                                         tGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAcccc                                             <  2:599890/101‑1 (MQ=255)
                                                          ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg                                            <  2:2972194/101‑1 (MQ=255)
                                                          ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg                                            <  1:3512693/101‑1 (MQ=255)
                                                          ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg                                            >  1:67425/1‑101 (MQ=255)
                                                          ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGGGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg                                            >  2:1845163/1‑101 (MQ=255)
                                                           gATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGa                                           >  1:3171510/1‑101 (MQ=255)
                                                             tCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAtt                                         <  1:2970235/101‑1 (MQ=255)
                                                               gAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACACCGAtttt                                       <  1:4218316/101‑1 (MQ=255)
                                                                aaaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGCTTTTc                                      >  2:3320016/1‑101 (MQ=255)
                                                                 acccgccgCCCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg                                     <  1:2902967/99‑1 (MQ=255)
                                                                 aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg                                     <  2:4691261/101‑1 (MQ=255)
                                                                 aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg                                     >  1:5224609/1‑101 (MQ=255)
                                                                   ccgccgCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGc                                   >  1:2115626/1‑101 (MQ=255)
                                                                    cgccgcGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGcc                                  >  2:1427812/1‑101 (MQ=255)
                                                                       cgcgCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCt                               >  2:357529/1‑101 (MQ=255)
                                                                        gcgcAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTg                              <  2:854930/101‑1 (MQ=255)
                                                                         cgcAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGa                             >  1:3738749/1‑101 (MQ=255)
                                                                           cAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgg                           <  2:504374/101‑1 (MQ=255)
                                                                           cAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgg                           <  2:4156830/101‑1 (MQ=255)
                                                                           cAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgg                           <  1:5505232/101‑1 (MQ=255)
                                                                            aaaaaGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCTGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAggg                          <  2:3962217/101‑1 (MQ=255)
                                                                            aaaaaGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAggg                          <  1:1046921/101‑1 (MQ=255)
                                                                             aaaaGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgggg                         <  1:51737/101‑1 (MQ=255)
                                                                              aaaGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGa                        <  1:539023/101‑1 (MQ=255)
                                                                              aaaGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGa                        <  1:2785032/101‑1 (MQ=255)
                                                                               aaGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGaa                       <  1:2453068/101‑1 (MQ=255)
                                                                                aGCCCGCCAAGAAGCGG..AGCA.GGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGaaa                      >  1:3281344/1‑101 (MQ=255)
                                                                                aGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGaaa                      >  2:1571752/1‑101 (MQ=255)
                                                                                aGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGaaa                      <  2:1122667/101‑1 (MQ=255)
                                                                                 gCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAg                     <  2:1351735/101‑1 (MQ=255)
                                                                                     gCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTc                 <  1:936842/101‑1 (MQ=255)
                                                                                     gCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTc                 >  1:1318197/1‑101 (MQ=255)
                                                                                       cAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCtt               >  1:1526009/1‑101 (MQ=255)
                                                                                       cAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCtt               <  1:1129853/101‑1 (MQ=255)
                                                                                       cAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCtt               <  2:3250709/101‑1 (MQ=255)
                                                                                         agaagCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTgg             <  1:3840792/101‑1 (MQ=255)
                                                                                           aagCGG..AGCC.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGca           <  2:4286140/101‑1 (MQ=255)
                                                                                            agCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcac          >  1:3097347/1‑101 (MQ=255)
                                                                                             gCGG..ATCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcaca         <  2:2579520/101‑1 (MQ=255)
                                                                                             gCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcaca         <  1:1802972/101‑1 (MQ=255)
                                                                                              cGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACaa        <  1:3934621/101‑1 (MQ=255)
                                                                                              cGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACACCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACaa        >  2:4811490/1‑101 (MQ=255)
                                                                                               gg..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATGGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAt       <  1:4962230/101‑1 (MQ=255)
                                                                                               gg..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAt       <  2:790027/101‑1 (MQ=255)
                                                                                                   aGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATTc     <  1:5083574/101‑1 (MQ=255)
                                                                                                     cA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATTCcg   <  2:2199458/101‑1 (MQ=255)
                                                                                                      a.aGTCGTTCCCCCCGCGGGGGCGCGGATCGAAAGCGGCGCATCGGCACCATAAACCCCGATTTTCGGCTCCTGAGGGGAAAGTTTCTTGAGAATATTCcgc  >  2:3471656/1‑101 (MQ=21)
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TCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATTCCGC  >  NC_014034/1397935‑1398135

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG