Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,891 +A 1.9% intergenic (‑144/+49) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8911.→A 1.9% 11.6 ‑4.3 ‑0.0268intergenic (‑144/+49)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (109/154):  total (114/154)
Fisher's exact test strand distribution p-value = 1.32e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.76e-01

Only 100 of 295 total aligned reads displayed.

AGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGC  >  NC_014034/1734792‑1734991
                                                                                                                                                                                                           
aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCGCCCGGGATATTTATGGCAAGATGAAAC.TGc                                                                                                       <  1:5149666/101‑1 (MQ=255)
     aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCt                                                                                                  >  2:3421811/1‑101 (MQ=255)
      aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTTTTGCAAGAAGAAACTTGCGGctt                                                                                                  >  2:1086292/1‑99 (MQ=17)
      aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCtt                                                                                                 <  1:5298167/101‑1 (MQ=255)
      aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCtt                                                                                                 <  2:2449335/101‑1 (MQ=255)
      aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCtt                                                                                                 <  2:3024282/101‑1 (MQ=255)
      aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCtt                                                                                                 <  1:1029121/101‑1 (MQ=255)
      aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCtt                                                                                                 >  2:4340228/1‑101 (MQ=255)
       ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTc                                                                                                <  1:5736881/101‑1 (MQ=255)
       ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAA.TGCGGCCTTc                                                                                                >  2:1979906/1‑101 (MQ=255)
         cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCAt                                                                                              >  1:1895732/1‑101 (MQ=255)
          tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATc                                                                                             <  2:842321/101‑1 (MQ=255)
           gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCt                                                                                            <  2:2571326/101‑1 (MQ=255)
           gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCt                                                                                            <  1:2740300/101‑1 (MQ=255)
           gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGACATTTATGGCAAGATGAAAC.TGGGGCCTTCATCt                                                                                            >  1:2215329/1‑101 (MQ=18)
            aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCtt                                                                                           <  2:1484211/101‑1 (MQ=255)
            aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTAATCtt                                                                                           >  2:2081575/1‑101 (MQ=255)
             cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTg                                                                                          >  2:2367474/1‑101 (MQ=255)
              gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGc                                                                                         >  2:2858121/1‑101 (MQ=255)
               cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGcc                                                                                        <  1:1138117/101‑1 (MQ=255)
                   gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATaa                                                                                    <  1:3429563/101‑1 (MQ=255)
                    tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATaaa                                                                                   <  1:656362/101‑1 (MQ=255)
                    tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATaaa                                                                                   <  2:1758141/101‑1 (MQ=255)
                     cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAat                                                                                  <  1:2956350/101‑1 (MQ=255)
                     cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAat                                                                                  >  2:5139625/1‑101 (MQ=255)
                     cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAat                                                                                  <  2:2763945/101‑1 (MQ=255)
                      aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAata                                                                                 >  2:1002499/1‑101 (MQ=255)
                      aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAata                                                                                 <  2:3660253/101‑1 (MQ=255)
                      aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAata                                                                                 >  1:2681179/1‑101 (MQ=255)
                       gCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAata.t                                                                               <  2:4751284/101‑1 (MQ=255)
                         ccaccggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.Tcc                                                                             <  2:4636348/96‑1 (MQ=255)
                         cGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.Tcc                                                                             <  2:3829598/101‑1 (MQ=255)
                           aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCg                                                                           <  2:3562984/101‑1 (MQ=255)
                           aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCg                                                                           <  2:4279709/101‑1 (MQ=255)
                               gccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGGTCTTTATCGCAAGATGGAAC.CGCGGGCCTCATCTTTCCCCAAAAATTCCCgggg                                                                        >  2:4890036/1‑101 (MQ=2)
                                  gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCg                                                                    >  1:912455/1‑101 (MQ=255)
                                    gcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCggg                                                                  <  1:1553536/101‑1 (MQ=255)
                                     gaGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCgggg                                                                 <  2:914900/100‑1 (MQ=255)
                                      aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGc                                                                >  1:5601957/1‑101 (MQ=255)
                                       ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCa                                                               <  1:3563679/101‑1 (MQ=255)
                                       ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCa                                                               <  2:656116/101‑1 (MQ=255)
                                       ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCa                                                               <  1:3409499/101‑1 (MQ=255)
                                       ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCa                                                               <  1:4002891/101‑1 (MQ=255)
                                         ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCaga                                                             <  2:453711/101‑1 (MQ=255)
                                          gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCCTAAATA.TCCCGGGGTGCGGGGCagag                                                            >  2:600081/1‑101 (MQ=255)
                                           ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGc                                                           <  1:1692893/101‑1 (MQ=255)
                                             cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGccc                                                         >  1:251495/1‑101 (MQ=255)
                                              gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGcccc                                                        <  2:1606945/96‑1 (MQ=255)
                                              gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGcccc                                                        >  2:2218998/6‑101 (MQ=255)
                                              cctgacgtgcGTCCTGCTGAAGCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGcccc                                                        <  2:3317340/92‑1 (MQ=21)
                                               ctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCg                                                       >  1:510128/5‑101 (MQ=255)
                                                tgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGc                                                      <  1:3102771/98‑1 (MQ=255)
                                                 gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCt                                                     <  2:4057148/99‑1 (MQ=255)
                                                 gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCt                                                     <  1:2016460/99‑1 (MQ=255)
                                                  ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTc                                                    >  1:2587863/2‑101 (MQ=255)
                                                   cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCa                                                   <  1:1737547/101‑1 (MQ=255)
                                                   cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCa                                                   >  2:4180449/1‑101 (MQ=255)
                                                   cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCa                                                   >  2:4451061/1‑101 (MQ=255)
                                                     cccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAAc                                                 <  1:1234628/101‑1 (MQ=255)
                                                         tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGaa                                             <  1:4340494/101‑1 (MQ=255)
                                                          ccTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAg                                            >  2:4995155/1‑101 (MQ=255)
                                                           cTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                                           >  2:1540280/1‑101 (MQ=255)
                                                           cTGCGGACTCCCCCCGGCATATTTATGGCAAGATGAAAC.TGCGGCCTTTATCTTGCCATAAATA.TCGCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                                           <  1:2425870/101‑1 (MQ=255)
                                                             gCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCGCCGCTCAACCGAACATg                                         >  2:39702/1‑101 (MQ=255)
                                                             gCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATg                                         <  1:2146076/101‑1 (MQ=255)
                                                               ggACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                       >  1:2372214/1‑101 (MQ=255)
                                                                gACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                      <  2:5757828/101‑1 (MQ=255)
                                                                gACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                      >  1:2865827/1‑101 (MQ=255)
                                                                  cTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                    <  2:3216675/101‑1 (MQ=255)
                                                                   tCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGa                                   >  1:87933/1‑101 (MQ=255)
                                                                    ccccccGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                  >  2:2640890/1‑101 (MQ=255)
                                                                     cccccGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                 <  1:3803851/101‑1 (MQ=255)
                                                                      ccccGGGATATTTCTGGCAAGATGAAAC.TGCGGTCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                                <  1:4973020/101‑1 (MQ=255)
                                                                      ccccGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                                <  2:2051655/101‑1 (MQ=255)
                                                                      ccccGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                                <  2:1895623/101‑1 (MQ=255)
                                                                         cGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                             <  1:1373541/101‑1 (MQ=255)
                                                                             atatTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                         >  1:4804160/1‑101 (MQ=255)
                                                                              tatTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                        <  2:3406058/101‑1 (MQ=255)
                                                                              tatTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                        <  2:5344932/101‑1 (MQ=255)
                                                                              tatTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                        >  1:5006912/1‑101 (MQ=255)
                                                                              tatTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                        >  2:2671399/1‑101 (MQ=255)
                                                                                tttATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                      >  2:731538/1‑101 (MQ=255)
                                                                                tttATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                      <  1:764445/101‑1 (MQ=255)
                                                                                tttATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                      <  1:4549970/101‑1 (MQ=255)
                                                                                 ttATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                     >  1:3496040/1‑101 (MQ=255)
                                                                                  tATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                    <  1:2265793/101‑1 (MQ=255)
                                                                                   aTGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                   >  1:5385380/1‑101 (MQ=255)
                                                                                    tGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                  <  2:1318391/101‑1 (MQ=255)
                                                                                    tGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                  >  1:3841626/1‑101 (MQ=255)
                                                                                     ggCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg                 <  1:2824175/101‑1 (MQ=255)
                                                                                         aGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtga             >  1:5481331/1‑101 (MQ=255)
                                                                                           aggAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg           <  1:2081692/99‑1 (MQ=255)
                                                                                             gAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg         >  1:441181/1‑101 (MQ=255)
                                                                                              aaaC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc        <  1:3309226/101‑1 (MQ=255)
                                                                                              aaaC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc        <  2:2528155/101‑1 (MQ=255)
                                                                                               aaC.TGCGGGCTTCACCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa       <  1:4995436/101‑1 (MQ=255)
                                                                                               aaC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa       <  1:474226/101‑1 (MQ=255)
                                                                                                aC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa      <  2:549752/101‑1 (MQ=255)
                                                                                                   tGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc    <  2:4369905/101‑1 (MQ=255)
                                                                                                   tGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc    <  2:1737746/101‑1 (MQ=255)
                                                                                                                                                                                                           
AGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAC.TGCGGCCTTCATCTTGCCATAAATA.TCCCGGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGC  >  NC_014034/1734792‑1734991

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG