BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,740,769	+G	97.8%	intergenic (+6/+129)	cxp → / ← RCAP_rcc01610	thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,740,769	1	.→G	97.8%	7.0 ‑4.2 ‑0.2	224	intergenic (+6/+129)	cxp/RCAP_rcc01610	thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand): new base (127/92): ref base (0/5): total (127/97)
Fisher's exact test strand distribution p-value = 1.43e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.62e-01

Only 100 of 279 total aligned reads displayed.


GCGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.GGGG.CGCTGCCCCCCGCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGG  >  NC_014034/1740669‑1740864


gggagaCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.a                                                                                                   <  1:1539632/99‑1 (MQ=255)


  gagaCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.gg                                                                                                >  2:5730655/1‑101 (MQ=255)


   agaCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.ggg                                                                                               >  1:4646454/1‑101 (MQ=255)


     gcGATCGTGCCCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.ggggg                                                                                             <  2:2409796/100‑2 (MQ=255)


     gcGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.ggggg                                                                                             <  2:813428/100‑2 (MQ=255)


     aCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.ggggg                                                                                             <  1:4237103/101‑2 (MQ=255)


     aCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.ggggg                                                                                             >  2:1488743/1‑100 (MQ=255)


        aTCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.gggggcgc                                                                                          >  1:3256954/1‑97 (MQ=255)


         tCGGGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.gggggcgct                                                                                         <  1:5551368/101‑6 (MQ=255)


           gTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGc                                                                                       >  2:4024979/1‑101 (MQ=255)


           gTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.gggggggcggc                                                                                       >  1:5051871/1‑94 (MQ=255)


            tGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGcc                                                                                      >  1:2213705/1‑101 (MQ=255)


             gccccccggCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGccc                                                                                     <  1:1028243/93‑1 (MQ=255)


             gACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGccc                                                                                     >  2:5334200/1‑101 (MQ=255)


               ccGCCTGGGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTAACTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGccccc                                                                                   <  2:4689852/101‑1 (MQ=255)


               ccGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGccccc                                                                                   <  1:5352493/101‑1 (MQ=255)


               ccGCCGGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGccccc                                                                                   <  2:4962608/101‑1 (MQ=255)


                cGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGcccccg                                                                                  >  1:3080468/1‑100 (MQ=255)


                   cTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.GGCTGcccccgccg                                                                               >  1:3160383/1‑97 (MQ=255)


                    tGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGcccacgccga                                                                              >  2:1078877/1‑94 (MQ=255)


                     gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAg                                                                             <  1:4706737/101‑1 (MQ=255)


                     gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAg                                                                             >  1:1723362/1‑101 (MQ=255)


                       gggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGc                                                                           >  2:3792543/1‑101 (MQ=255)


                        ggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCg                                                                          <  2:617640/101‑1 (MQ=255)


                        ggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCg                                                                          <  1:4571717/101‑1 (MQ=255)


                         gTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGGTGCCCCCGCCGAGGCGa                                                                         >  2:3902201/1‑101 (MQ=255)


                         gTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGa                                                                         <  2:8409/101‑1 (MQ=255)


                          ttCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGAt                                                                        >  1:2053441/1‑101 (MQ=255)


                           tCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATg                                                                       >  1:3246489/1‑101 (MQ=255)


                            cGAGCCGACGGAAGAACCGTTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGc                                                                      <  1:3418281/101‑1 (MQ=255)


                            cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGc                                                                      >  2:3945423/1‑101 (MQ=255)


                            cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGc                                                                      <  1:3605297/101‑1 (MQ=255)


                                     ggAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGcacc                                                             >  2:5553025/1‑101 (MQ=255)


                                     ggAAGAACCGCTTCTGGACTACCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGcccc                                                             <  1:3675765/101‑1 (MQ=255)


                                      gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGcccca                                                            <  2:3238418/101‑2 (MQ=255)


                                       aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGcccccc                                                           <  2:5079368/101‑1 (MQ=255)


                                       aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGcccccc                                                           >  1:608370/1‑101 (MQ=255)


                                        agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCg                                                          <  1:882560/101‑1 (MQ=255)


                                        agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCg                                                          <  1:5604384/101‑1 (MQ=255)


                                         gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCgg                                                         >  2:560806/1‑101 (MQ=255)


                                          aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCggg                                                        >  2:352278/1‑101 (MQ=255)


                                           aCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGa                                                       >  1:733613/1‑101 (MQ=255)


                                             cGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACAGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGata                                                     >  2:1011483/1‑101 (MQ=255)


                                              gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGatat                                                    <  1:923906/101‑1 (MQ=38)


                                               cgctcttccgaTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                   <  2:5258115/91‑1 (MQ=37)


                                               cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                   >  2:2986533/1‑101 (MQ=38)


                                               cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                   >  2:4836457/1‑101 (MQ=38)


                                                ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGCGGCGATGCCTCGGCCCCCCGGGATAttt                                                  >  1:2706127/1‑101 (MQ=38)


                                                ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCggcacatgt                                                  >  1:2852047/1‑94 (MQ=255)


                                                ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                  <  2:1372545/101‑1 (MQ=38)


                                                 tCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                 <  1:4315895/101‑1 (MQ=38)


                                                 tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGCGG.G.GGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                 <  1:835967/101‑1 (MQ=38)


                                                 tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                 >  1:188732/1‑101 (MQ=38)


                                                    ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAg                                              <  2:126310/101‑1 (MQ=38)


                                                     gACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                             <  2:4347536/101‑1 (MQ=38)


                                                     gACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                             >  2:4890993/1‑101 (MQ=38)


                                                     gACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                             >  1:5247731/1‑101 (MQ=38)


                                                       cTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                           <  2:2568043/101‑1 (MQ=37)


                                                         aTCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                         <  1:1444111/101‑1 (MQ=37)


                                                         aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                         >  2:5222222/1‑101 (MQ=38)


                                                         aTCTAGAAAGCAAATACGCCGCGATCTACCGTCTTGCGGGGC.G.AGGGGG.CTCTCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                         <  2:1937319/101‑1 (MQ=21)


                                                           ctcGAGAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCGGAGGCGATGCCTCGGCCCCCCGGCCTATTTCGAGCAAGATg                                       >  2:5752963/1‑101 (MQ=255)


                                                            tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                      >  2:3215392/1‑101 (MQ=37)


                                                            tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                      <  1:3792785/101‑1 (MQ=37)


                                                             cGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                     <  2:3438460/101‑1 (MQ=37)


                                                             cGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                     <  2:2022503/101‑1 (MQ=37)


                                                             cGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGAAATTTCGAGCAAGATGaa                                     >  1:3466365/1‑101 (MQ=255)


                                                              gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                    >  2:2221685/1‑101 (MQ=37)


                                                              gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                    <  2:4568028/101‑1 (MQ=38)


                                                              gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                    <  1:959064/101‑1 (MQ=38)


                                                               aacgCAAATGCCCCGCGATCCACCGGCGTTGGGGGC.GTG.GGGG.CCCGGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                   <  2:4480002/98‑1 (MQ=16)


                                                               aaaGCAAATCCGCCGCGATCTCCGGGCTTGGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                   <  2:5051587/101‑1 (MQ=25)


                                                                aaGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCCGGGTATTTCGAGCAAGATGAAAgg                                  >  2:3090717/1‑101 (MQ=255)


                                                                  gCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                                >  1:101253/1‑101 (MQ=37)


                                                                     aaTACGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATcc                             >  1:1090712/1‑101 (MQ=37)


                                                                        aCGCCGCGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGt                          >  1:4010385/1‑101 (MQ=37)


                                                                          gccgcGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                        <  2:551941/101‑1 (MQ=37)


                                                                          gccgcGATCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                        <  2:1167464/101‑1 (MQ=37)


                                                                                aTCTCCCGGCTTTGCGGGC.G.C.GGGGCCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                  <  2:2851886/101‑1 (MQ=21)


                                                                                aTCTCCCGGCTTTGAGGGCGG.G.GGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                  <  1:564364/101‑1 (MQ=25)


                                                                                aTCTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                  >  2:1666303/1‑101 (MQ=25)


                                                                                 tCTCCCGGCTTGGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                 <  2:2607526/101‑1 (MQ=25)


                                                                                  cTACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                <  1:213216/101‑1 (MQ=25)


                                                                                   tACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCttaga               >  1:784389/1‑98 (MQ=25)


                                                                                   tACCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCt               <  2:2437615/101‑1 (MQ=25)


                                                                                    aCCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt              >  2:1728068/1‑101 (MQ=25)


                                                                                    aCCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt              >  1:4334535/1‑101 (MQ=25)


                                                                                    aCCGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt              >  2:2814278/1‑101 (MQ=25)


                                                                                      cGGCTTTGGGGGCGG.G.GGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCa            <  1:2419012/101‑1 (MQ=21)


                                                                                      cGGCTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCa            >  2:1825264/1‑101 (MQ=25)


                                                                                         gtttGAGGGC.G.AGGGGG.CGTTGCCCCCGCCGAGGCGATGCCTCGGCGCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt         <  1:2538158/100‑1 (MQ=21)


                                                                                         cTTTGAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt         >  1:1828392/1‑101 (MQ=25)


                                                                                          ttggggggC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa        <  1:1319708/96‑1 (MQ=21)


                                                                                             gAGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt     >  2:3658834/1‑101 (MQ=25)


                                                                                              ggggC.G.AGGGGG.CGCTCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc    <  1:4446878/100‑1 (MQ=21)


                                                                                              aGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc    >  1:2794089/1‑101 (MQ=25)


                                                                                              aGGGC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc    >  2:5458939/1‑101 (MQ=25)


                                                                                               gggC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCg   >  1:5295024/1‑101 (MQ=25)


                                                                                                ggC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgg  <  2:1317999/101‑1 (MQ=25)


                                                                                                ggC.G.AGGGGG.CGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgg  >  2:2744198/1‑101 (MQ=25)


GCGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.G.A.GGGG.CGCTGCCCCCCGCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGG  >  NC_014034/1740669‑1740864

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG