Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,740,785 +C 1.9% intergenic (+22/+113) cxp → / ← RCAP_rcc01610 thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,740,7851.→C 1.9% 7.1 ‑2.8 ‑0.0265intergenic (+22/+113)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/5):  ref base (127/133):  total (127/138)
Fisher's exact test strand distribution p-value = 6.12e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.92e-01

Only 100 of 282 total aligned reads displayed.

GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGGG.CGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740690‑1740880
                                                                                                   |                                                                                               
gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAg                                                                                             <  1:4706737/101‑1 (MQ=255)
 ggggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAgg                                                                                            <  1:1269705/100‑1 (MQ=255)
  gggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGc                                                                                           >  2:3654472/1‑101 (MQ=255)
   ggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCg                                                                                          <  2:617640/101‑1 (MQ=255)
   ggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCg                                                                                          <  1:4571717/101‑1 (MQ=255)
   ggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCCCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCg                                                                                          <  2:1287271/101‑1 (MQ=255)
    gTTGGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGa                                                                                         <  1:1838129/101‑1 (MQ=255)
       cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGc                                                                                      >  2:3945423/1‑101 (MQ=255)
        gAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGcc                                                                                     >  2:3791043/1‑101 (MQ=255)
          gCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTc                                                                                   <  1:3197336/101‑1 (MQ=255)
             gACGGAAGACCCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGc                                                                                <  2:3997948/101‑1 (MQ=255)
             gACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGc                                                                                >  1:5271123/1‑101 (MQ=255)
              aCGGAAGAACCGCTTCTGGACTATCTCGAATGCAAATACGCCGAGATCGACCGAGTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcc                                                                               <  2:4790104/101‑1 (MQ=255)
               cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGccc                                                                              >  2:5585261/1‑101 (MQ=255)
                ggAAGAACCGCTTCTGGACTACCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcccc                                                                             <  1:3675765/101‑1 (MQ=255)
                 gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcccca                                                                            <  2:3238418/101‑2 (MQ=255)
                  aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                           >  1:1353106/1‑101 (MQ=255)
                    gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgg                                                                         >  2:560806/1‑101 (MQ=255)
                     aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                                        <  2:3458231/101‑1 (MQ=255)
                      aCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGa                                                                       <  2:5270833/101‑1 (MQ=255)
                           ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                                  >  2:1756919/1‑101 (MQ=38)
                           ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                                  <  2:1372545/101‑1 (MQ=38)
                               ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAg                                                              <  1:4037712/101‑1 (MQ=38)
                               ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAg                                                              >  2:1111110/1‑101 (MQ=38)
                               ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAg                                                              >  1:1013691/1‑101 (MQ=38)
                               ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGCGC.GAGGGGG.CGCTGCGCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATACTTCgcg                                                              >  1:5751567/1‑99 (MQ=255)
                                gACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                                             <  2:4347536/101‑1 (MQ=38)
                                gACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                                             >  1:5247731/1‑101 (MQ=38)
                                gACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                                             >  2:4890993/1‑101 (MQ=38)
                                  cTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                                           <  2:2568043/101‑1 (MQ=37)
                                  cTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                                           <  1:5320539/101‑1 (MQ=38)
                                  cTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGACGCCTCGGCCCCCCGGGATATTTCGAGCaa                                                           >  1:5582690/1‑101 (MQ=38)
                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                          >  2:3507754/1‑101 (MQ=38)
                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGCATATTTCGAGCCAg                                                          >  1:5623994/1‑101 (MQ=255)
                                    aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                                         <  2:4646178/101‑1 (MQ=38)
                                    aTCTAGAAAGCAAATACGCCGCGATCTACCGTCTTGCGGGGC.GAGGGGG.CTCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                                         <  2:1937319/101‑1 (MQ=21)
                                      ctcGAGAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.GGAGGCGATGCCTCGGCCCCCCGGCCTATTTCGAGCAAGATg                                                       >  2:5752963/1‑101 (MQ=255)
                                       tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                                      <  1:3792785/101‑1 (MQ=37)
                                       tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                                      >  2:3215392/1‑101 (MQ=37)
                                       tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                                      >  2:5119069/1‑101 (MQ=37)
                                        cGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                                     >  1:877391/1‑101 (MQ=37)
                                        cGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGAAATTTCGAGCAAGATGaa                                                     >  1:3466365/1‑101 (MQ=255)
                                         gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    >  2:2221685/1‑101 (MQ=37)
                                         gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    >  2:1177622/1‑101 (MQ=37)
                                          aaaGCAAATCCGCCGCGATCTCCGGGCTTGGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                                   <  2:5051587/101‑1 (MQ=25)
                                           aaGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAgg                                                  >  2:4520616/1‑101 (MQ=37)
                                           aaGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCCGGGTATTTCGAGCAAGATGAAAgg                                                  >  2:3090717/1‑101 (MQ=255)
                                            aGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                                 <  1:905504/101‑1 (MQ=38)
                                                aaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATcc                                             >  1:1090712/1‑101 (MQ=37)
                                                aaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATcc                                             >  1:3408304/1‑101 (MQ=37)
                                                  tACGCCGCGATCTACCGGCTTGGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCgg                                           <  1:937358/101‑1 (MQ=37)
                                                    cgccgcGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGtt                                         <  1:2043482/101‑1 (MQ=37)
                                                     gccgcGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                                        <  2:551941/101‑1 (MQ=37)
                                                      ccgcGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTc                                       >  2:1697353/1‑101 (MQ=37)
                                                          gATCTACCGGCTTTGAGGGC.GAGGGGG.CGGTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCt                                   <  1:2394703/101‑1 (MQ=25)
                                                           tcttACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  <  2:784321/98‑1 (MQ=25)
                                                           aTCTCCCGGCTTTGGGGGCGGG.GGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  <  2:2812813/101‑1 (MQ=21)
                                                           aTCTCCCGGCTTTGCGGGC.GC.GGGGCCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  <  2:2851886/101‑1 (MQ=21)
                                                           aTCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  >  1:2683561/1‑101 (MQ=25)
                                                           aTCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  >  1:4384154/1‑101 (MQ=25)
                                                            tCTCCCGGCTTGGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                                 <  2:2607526/101‑1 (MQ=25)
                                                            tCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                                 <  1:429124/101‑1 (MQ=25)
                                                             cTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                                >  2:5162650/1‑101 (MQ=25)
                                                             cTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                                <  1:213216/101‑1 (MQ=25)
                                                              tACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCttaga                               >  1:784389/1‑98 (MQ=25)
                                                               cccGGCTTTGAGGGC.GA.GGGGCCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt                              <  2:5231489/100‑1 (MQ=25)
                                                               aCCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt                              >  1:4334535/1‑101 (MQ=25)
                                                                 cGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCa                            >  2:1825264/1‑101 (MQ=25)
                                                                 cGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCa                            <  1:664859/101‑1 (MQ=25)
                                                                  ggCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCaa                           >  1:2872226/1‑101 (MQ=25)
                                                                    cTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt                         >  1:1611222/1‑101 (MQ=25)
                                                                     tttGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa                        >  2:2985178/1‑101 (MQ=25)
                                                                     tttGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa                        >  1:3247030/1‑101 (MQ=25)
                                                                       tGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAcc                      >  1:997135/1‑101 (MQ=25)
                                                                        gAGGGC.GG.GGGG.GCCCGCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt                     <  1:2463724/101‑1 (MQ=21)
                                                                         ggggggggggggg.CGGGGCCCCCCC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt                     <  1:3094195/92‑1 (MQ=21)
                                                                         gaggggggggggg.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt                     <  2:3810514/92‑1 (MQ=21)
                                                                         aGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc                    >  1:2794089/1‑101 (MQ=25)
                                                                         aGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc                    >  2:5458939/1‑101 (MQ=25)
                                                                           ggC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgg                  <  2:1501004/101‑1 (MQ=25)
                                                                               ggggggg.CGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg               <  1:2173540/98‑1 (MQ=21)
                                                                               gaggggg.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg               >  1:5516190/4‑101 (MQ=255)
                                                                                gggggg.CGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgggggg              <  1:282599/99‑1 (MQ=255)
                                                                                 ggggg.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGa             <  2:2434611/100‑1 (MQ=255)
                                                                                  gggg.CGCTGCCCCCGC.CGAGGCGATGCCTCTGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAg            <  1:352322/101‑1 (MQ=255)
                                                                                  gggg.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAg            <  1:3068064/101‑1 (MQ=255)
                                                                                       cgcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTccc        <  1:1488836/101‑1 (MQ=255)
                                                                                        gcTGTCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg       <  1:3420546/101‑1 (MQ=255)
                                                                                        gcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg       >  2:1015385/1‑101 (MQ=255)
                                                                                         cTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGc      >  1:834061/1‑101 (MQ=255)
                                                                                          tGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCa     >  1:3637556/1‑101 (MQ=255)
                                                                                          tGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCa     <  1:1011558/101‑1 (MQ=255)
                                                                                          tGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCCTGCTTCAAATACCTCGGGGGGAGTCCCGCa     >  1:396505/1‑101 (MQ=255)
                                                                                           gCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAg    <  1:5056000/101‑1 (MQ=255)
                                                                                           gCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAg    >  1:4184488/1‑101 (MQ=255)
                                                                                           gCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAg    <  1:1740363/101‑1 (MQ=255)
                                                                                            cccccgC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAgg   <  2:2454/101‑1 (MQ=255)
                                                                                            cccccgC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAgg   >  2:1260158/1‑101 (MQ=255)
                                                                                            ccccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAg    <  2:857185/101‑1 (MQ=255)
                                                                                              ccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAggg  <  1:5730950/101‑1 (MQ=255)
                                                                                                   |                                                                                               
GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGGG.CGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740690‑1740880

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG