Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,769,370 +G 91.8% coding (442/744 nt) RCAP_rcc01636 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,769,3701.→G91.8% 40.8 ‑0.9 ‑0.1183coding (442/744 nt)RCAP_rcc01636hypothetical protein
Reads supporting (aligned to +/- strand):  new base (79/89):  ref base (5/10):  total (84/99)
Fisher's exact test strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.89e-01

Only 100 of 202 total aligned reads displayed.

TGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTC.GGCA.GGGGCGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCC  >  NC_014034/1769272‑1769461
                                                                                                     |                                                                                             
tgtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTc                                                                                                <  2:1613062/101‑1 (MQ=255)
tgtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTc                                                                                                <  2:2552894/101‑1 (MQ=255)
    cGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTcgcgc                                                                                            <  1:2156765/101‑5 (MQ=255)
        aGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTcgcgcgcgg                                                                                        <  2:3171509/101‑9 (MQ=255)
             cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCttt                                                                                 <  1:351937/101‑1 (MQ=255)
             cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCttt                                                                                 <  1:2453841/101‑1 (MQ=255)
             cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCttt                                                                                 <  1:355587/101‑1 (MQ=255)
             cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCttt                                                                                 <  1:1846145/101‑1 (MQ=255)
                 gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCt                                                                             >  1:3754741/1‑101 (MQ=255)
                 gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCt                                                                             >  1:310880/1‑101 (MQ=255)
                  aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCtt                                                                            <  2:387670/101‑1 (MQ=255)
                  aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCtt                                                                            >  2:38452/1‑101 (MQ=255)
                   tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTg                                                                           <  1:4625480/101‑1 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTgg                                                                          <  2:3572878/101‑1 (MQ=255)
                     aGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTggg                                                                         <  2:3112281/101‑1 (MQ=255)
                     aGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTggg                                                                         >  1:613625/1‑101 (MQ=255)
                     aGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTggg                                                                         >  1:3202485/1‑101 (MQ=255)
                     aGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTggg                                                                         <  2:5443523/101‑1 (MQ=255)
                      ggACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTgggg                                                                        >  2:3046432/1‑101 (MQ=255)
                          cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGa                                                                    <  1:2565536/101‑1 (MQ=255)
                           aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAg                                                                   <  1:5730762/101‑1 (MQ=255)
                            aCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAgg                                                                  <  2:3338549/101‑1 (MQ=255)
                             cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgc                                                                 >  2:653241/1‑101 (MQ=255)
                             cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgc                                                                 <  1:21380/101‑1 (MQ=255)
                              tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                                <  2:1925879/101‑1 (MQ=255)
                               ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcgc                                                               >  2:4563401/1‑101 (MQ=255)
                                 aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                             >  2:1390413/1‑101 (MQ=255)
                                 aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                             >  1:3837939/1‑101 (MQ=255)
                                  tGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTc                                                            >  1:2009645/1‑101 (MQ=255)
                                   gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                           <  1:889135/101‑1 (MQ=255)
                                     ggCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCgg                                                         <  2:1991443/101‑1 (MQ=255)
                                      gCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCggg                                                        >  1:865169/1‑101 (MQ=255)
                                       cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGCGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                                       >  1:347681/1‑101 (MQ=255)
                                         cgcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAc                                                     >  2:4029646/1‑101 (MQ=255)
                                         cgcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAc                                                     <  1:4081623/101‑1 (MQ=255)
                                         cgcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAc                                                     >  2:1499586/1‑101 (MQ=255)
                                         cgcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAc                                                     <  1:3632241/101‑1 (MQ=255)
                                           cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCg                                                   >  2:2448888/1‑101 (MQ=255)
                                            gcggcgGAAATCGTCGCGGCTTTGGGCGCGGTCTATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGa                                                  <  1:4010818/101‑1 (MQ=255)
                                            gcggcgGAAATCGCCGCGGCTTTGGGCGCGGTCTATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGa                                                  <  1:4979727/101‑1 (MQ=255)
                                             cggcgtAAGTCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                 <  2:2645279/101‑1 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                 <  1:2961750/101‑1 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                 <  2:411480/101‑1 (MQ=255)
                                               gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                               >  1:326987/1‑101 (MQ=255)
                                                cggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcg                                              <  1:4446184/101‑1 (MQ=255)
                                                  gAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                            >  2:5138184/1‑101 (MQ=255)
                                                  gAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                            >  2:2341910/1‑101 (MQ=255)
                                                  gAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                            >  1:2622314/1‑101 (MQ=255)
                                                   aaaTCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                           >  2:2143648/1‑101 (MQ=255)
                                                    aaTCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTcc                                          <  2:1152047/101‑1 (MQ=255)
                                                      tcgtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCtt                                        <  1:136621/101‑1 (MQ=255)
                                                          cgcgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                    >  2:4463174/1‑101 (MQ=255)
                                                          cgcgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                    >  2:1285930/1‑101 (MQ=255)
                                                           gcgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                   <  2:221277/101‑1 (MQ=255)
                                                            cgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                  <  1:1331546/101‑1 (MQ=255)
                                                            cgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                  <  1:2205966/101‑1 (MQ=255)
                                                             ggCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                 <  1:5364180/101‑1 (MQ=255)
                                                              gCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgt                                <  1:4369103/101‑1 (MQ=255)
                                                               cTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtc                               <  1:948903/101‑1 (MQ=255)
                                                                tttGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                              >  2:4272642/1‑101 (MQ=255)
                                                                tttGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                              <  2:4231840/101‑1 (MQ=255)
                                                                 ttGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgc                             <  2:2523010/101‑1 (MQ=255)
                                                                  tGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                            >  2:4316544/1‑101 (MQ=255)
                                                                  tGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                            >  2:2418383/1‑101 (MQ=255)
                                                                   gggCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccg                           <  1:1499679/101‑1 (MQ=255)
                                                                   gggCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccg                           <  2:613567/101‑1 (MQ=255)
                                                                    ggCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccgc                          <  1:5634481/101‑1 (MQ=255)
                                                                    ggCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccgc                          <  2:5129551/101‑1 (MQ=255)
                                                                        cgGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                      >  1:5168383/1‑101 (MQ=255)
                                                                        cgGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                      <  1:653299/101‑1 (MQ=255)
                                                                          gTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                    >  1:4232367/1‑101 (MQ=255)
                                                                           tCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                   >  2:170930/1‑101 (MQ=255)
                                                                             gATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg                 <  2:107375/101‑1 (MQ=255)
                                                                               tgtcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGc               <  2:3918867/91‑1 (MQ=255)
                                                                                gtcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCt              <  2:3768167/92‑1 (MQ=255)
                                                                                gtcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTACGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCt              <  2:4996548/92‑1 (MQ=255)
                                                                                 tcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTg             <  2:4720018/93‑1 (MQ=255)
                                                                                 tcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTg             <  1:1017252/93‑1 (MQ=255)
                                                                                   gggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt           <  2:5596662/95‑1 (MQ=255)
                                                                                   gggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt           <  2:5730584/95‑1 (MQ=255)
                                                                                   gggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt           >  1:2686958/7‑101 (MQ=255)
                                                                                   gggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt           <  1:3951720/95‑1 (MQ=255)
                                                                                    ggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtg          >  1:1146441/6‑101 (MQ=255)
                                                                                    ggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtg          >  2:2574840/6‑101 (MQ=255)
                                                                                    ggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtg          >  2:4618173/6‑101 (MQ=255)
                                                                                    ggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtg          >  1:2094252/6‑101 (MQ=255)
                                                                                    ggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtg          <  1:2103101/96‑1 (MQ=255)
                                                                                     gcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc         <  2:2002990/97‑1 (MQ=255)
                                                                                      cagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc        >  1:4826003/4‑101 (MQ=255)
                                                                                       agggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg       >  2:3138847/3‑101 (MQ=255)
                                                                                       agggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg       <  2:4134361/99‑1 (MQ=255)
                                                                                       agggggCGGTGGTCGCG.CG.CGGGCTTGGCCTTGGCGTCGAGGCGCGCCCGGGCACCGATGCGCTCCTTGATTCCGGCGCCGCGCATCGCGGGCTGGtgccg       <  2:5448888/99‑1 (MQ=255)
                                                                                         ggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat     <  2:2148397/101‑1 (MQ=255)
                                                                                         ggggCGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat     >  1:140498/1‑101 (MQ=255)
                                                                                          gggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGAGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatc    >  1:2818652/1‑100 (MQ=255)
                                                                                          gggCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatg    >  2:2708517/1‑101 (MQ=255)
                                                                                           ggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgc   <  1:3084131/101‑1 (MQ=255)
                                                                                           ggCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgc   >  1:1388766/1‑101 (MQ=255)
                                                                                            gCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgcc  <  2:1963907/101‑1 (MQ=255)
                                                                                            gCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgcc  <  2:1367421/101‑1 (MQ=255)
                                                                                                     |                                                                                             
TGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTC.GGCA.GGGGCGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCC  >  NC_014034/1769272‑1769461

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG