Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,974,330 +G 100% intergenic (‑23/+32) rpe1 ← / ← cbbM ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,974,3301.→G100.0% 943.8 252intergenic (‑23/+32)rpe1/cbbMribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand):  new base (126/124):  ref base (0/2):  total (126/126)

Only 100 of 286 total aligned reads displayed.

GGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..G.GGC..GGGGTGT.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGG  >  NC_014034/1974232‑1974419
                                                                                                      |                                                                                            
   tttCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGC..gggg                                                                                         <  1:2594166/101‑1 (MQ=255)
   tttCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGC..gggg                                                                                         <  1:651579/101‑1 (MQ=255)
     tCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGC..gggggg                                                                                       >  2:1233478/1‑99 (MQ=255)
        cGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGC..gggggtgtc                                                                                    <  1:5625597/101‑6 (MQ=255)
          gCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGT.c.cc                                                                                 <  2:3400621/101‑1 (MQ=255)
           ccGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGC..gggggggtcccc                                                                                 >  1:2256603/1‑93 (MQ=255)
            cGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGT.c.cccc                                                                               <  1:3942459/101‑1 (MQ=255)
             gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGT.c.ccccc                                                                              <  2:5414217/101‑2 (MQ=255)
             gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGT.c.ccccc                                                                              <  2:5234519/101‑2 (MQ=255)
             gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGT.c.ccccc                                                                              >  2:4283352/1‑100 (MQ=255)
             gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGC..ggggggggaccccc                                                                               >  2:5071234/1‑91 (MQ=255)
              aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGT.c.cccccg                                                                             <  1:1886055/101‑3 (MQ=255)
              aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGT.c.cccccg                                                                             <  2:5557895/101‑3 (MQ=255)
                 gcGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGT.c.cccccgccc                                                                          <  1:2351904/100‑6 (MQ=255)
                  cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.Ccccgcccg                                                                          <  1:919509/101‑1 (MQ=255)
                  cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGT.c.cccccgcgcg                                                                         >  2:4879737/1‑95 (MQ=255)
                     cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGAt                                                                       <  1:2022029/101‑1 (MQ=255)
                     cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTCGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGAt                                                                       <  1:1019920/101‑1 (MQ=255)
                      gAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGGGGGGTTG.G.GGCG.GGGGGGT.CGCCCCGCCCGGATa                                                                      >  1:4969997/1‑101 (MQ=255)
                      gAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATa                                                                      <  2:406254/101‑1 (MQ=255)
                        aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGc                                                                    <  1:4223736/101‑1 (MQ=255)
                        aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGc                                                                    <  2:5135986/101‑1 (MQ=255)
                        aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGc                                                                    <  2:238689/101‑1 (MQ=255)
                        aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGGGTCC.CCCCGCCCGGATAGc                                                                    >  2:2730690/1‑101 (MQ=255)
                         gTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCa                                                                   <  2:4088466/101‑1 (MQ=255)
                         gTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCa                                                                   <  1:3464533/101‑1 (MQ=255)
                          tCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCAc                                                                  <  1:5292656/101‑1 (MQ=255)
                           cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCAcg                                                                 <  2:837211/101‑2 (MQ=255)
                           cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCAcg                                                                 >  1:1839852/1‑100 (MQ=255)
                            ggCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGATCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCAcgg                                                                >  2:1930020/1‑99 (MQ=255)
                             gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCAcggc                                                               >  2:1905876/1‑98 (MQ=255)
                             gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGGGTCC.CCCCGCCCGGATAGCAcggc                                                               >  2:4236895/1‑98 (MQ=255)
                              gaGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCAcggcg                                                              <  2:3590580/100‑5 (MQ=255)
                              cAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGGGTCC.CCCCGCCCGGATAGCAcggcg                                                              >  1:124275/1‑97 (MQ=255)
                              cAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGGGTCC.CCCCGCCCGGATAGCAcggcg                                                              >  1:2946703/1‑97 (MQ=255)
                               aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGGGTCC.CCCCGCCCGGATAGCA.CGGCGt                                                            >  2:1660988/1‑101 (MQ=255)
                                gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGGGTCC.CCCCGCCCGGGTAGCA.CGGCGtt                                                           >  1:5054236/1‑101 (MQ=255)
                                 aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGGGGC.C.CCCCGCCCGGATAGCA.CGGCGTTc                                                          >  2:1124476/1‑101 (MQ=255)
                                  aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                         <  2:2962019/101‑1 (MQ=255)
                                  aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                         <  1:753339/101‑1 (MQ=255)
                                   aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAg                                                        <  1:779642/101‑1 (MQ=255)
                                   aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAg                                                        <  1:2449047/101‑1 (MQ=255)
                                   aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAg                                                        >  1:544333/1‑101 (MQ=255)
                                     gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGG.GGG..GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                      <  1:3004621/101‑1 (MQ=255)
                                     gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                      <  2:1467343/101‑1 (MQ=255)
                                       tCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGttca                                                    <  2:2316021/101‑3 (MQ=255)
                                         gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGGTA.CGACGGCGTTCAGTTCACg                                                  >  2:2640819/1‑101 (MQ=255)
                                         gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                  <  1:2896938/101‑1 (MQ=255)
                                         gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                  <  1:3854885/101‑1 (MQ=255)
                                           gggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGcc                                                <  2:5313494/101‑1 (MQ=255)
                                            ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                               <  2:4157359/101‑1 (MQ=255)
                                             gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                              >  1:1583306/1‑101 (MQ=255)
                                              ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                             >  2:3496405/1‑101 (MQ=255)
                                               gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                            <  2:1839742/101‑1 (MQ=255)
                                                cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                           >  1:979847/1‑101 (MQ=255)
                                                 gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                          <  1:3081771/101‑1 (MQ=255)
                                                 gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                          <  2:1716698/101‑1 (MQ=255)
                                                  aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGGGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                         >  1:5105596/1‑101 (MQ=255)
                                                   tCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGa                                        >  2:770217/1‑101 (MQ=255)
                                                   tCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGa                                        >  1:5265586/1‑101 (MQ=255)
                                                    cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                       >  2:3659921/1‑101 (MQ=255)
                                                    cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                       <  1:1462055/101‑1 (MQ=255)
                                                    cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                       >  1:3387931/1‑101 (MQ=255)
                                                    cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                       <  1:4630528/101‑1 (MQ=255)
                                                     ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                      >  2:5043383/1‑101 (MQ=255)
                                                      tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                     >  1:4527307/1‑101 (MQ=255)
                                                       gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                    >  2:3952579/1‑101 (MQ=255)
                                                         tGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGcc                                  >  2:5749023/1‑101 (MQ=255)
                                                         tGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGGGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGcc                                  >  1:1494085/1‑101 (MQ=255)
                                                          ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                 <  2:4931913/101‑1 (MQ=255)
                                                          ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                 <  1:482225/101‑1 (MQ=255)
                                                               ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                            >  2:4533000/1‑101 (MQ=255)
                                                               ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                            >  2:3345307/1‑101 (MQ=255)
                                                                gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                           >  2:3717048/1‑101 (MQ=255)
                                                                gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                           >  2:4809834/1‑101 (MQ=255)
                                                                   gAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                        <  1:4013658/101‑1 (MQ=255)
                                                                    aaGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                       >  1:3498269/1‑101 (MQ=255)
                                                                    aaGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                       <  2:200342/101‑1 (MQ=255)
                                                                    aaGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                       <  1:3573252/101‑1 (MQ=255)
                                                                       gTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGc                    <  1:3208385/101‑1 (MQ=255)
                                                                       gTCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGc                    >  1:5788059/1‑101 (MQ=255)
                                                                        tCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCCGAGCGACGCGCCAGCCCGGATAGAGCt                   >  2:4713743/1‑101 (MQ=255)
                                                                        tCATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCt                   >  1:4144786/1‑101 (MQ=255)
                                                                         cATGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                  <  1:110402/101‑1 (MQ=255)
                                                                           tGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                >  2:90360/1‑101 (MQ=255)
                                                                           tGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                <  1:3268409/101‑1 (MQ=255)
                                                                           tGTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCAAGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                >  2:3243091/1‑101 (MQ=255)
                                                                            gTCGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTc               <  2:1551641/101‑1 (MQ=255)
                                                                              cGGTCTCCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGGCgg             >  2:1320598/1‑101 (MQ=255)
                                                                              cGGTCTCCTGAGGGGTT..GTGGCGGGGGGGGG.C.CCCCCCCCGGGTAG.AACGGCGGTTAGTTCACCCCCAGAGCGACGCCCCAGCCCGGGTAGAGCTGGTCgg             >  1:4297026/1‑101 (MQ=255)
                                                                                 tctcCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCAt          >  1:2799343/1‑101 (MQ=255)
                                                                                   tcCTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg        >  2:2826399/1‑101 (MQ=255)
                                                                                     cTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt      <  2:2459912/101‑1 (MQ=255)
                                                                                     cTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt      >  1:2199992/1‑101 (MQ=255)
                                                                                     cTGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt      <  1:3717276/101‑1 (MQ=255)
                                                                                      tGAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc     >  2:610669/1‑101 (MQ=255)
                                                                                       gAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg    >  1:2680811/1‑101 (MQ=255)
                                                                                       gAGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg    >  1:4671143/1‑101 (MQ=255)
                                                                                        aGGGGTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg   >  1:1313392/1‑101 (MQ=255)
                                                                                         ggggTTG.G.GGCG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg  <  1:2730839/101‑1 (MQ=255)
                                                                                                      |                                                                                            
GGATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..G.GGC..GGGGTGT.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGG  >  NC_014034/1974232‑1974419

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG