Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,047,133 +G 100% intergenic (‑24/+144) potH1 ← / ← RCAP_rcc01896 polyamine ABC transporter permease PotH/GntR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,047,1331.→G100.0% 1137.1 295intergenic (‑24/+144)potH1/RCAP_rcc01896polyamine ABC transporter permease PotH/GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (144/151):  ref base (0/0):  total (144/151)

Only 100 of 316 total aligned reads displayed.

CAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CC.A.CCG.CGGG.TGGAGATGCCGACATACTCTATTCT.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTCCCGCGATTGCC  >  NC_014034/2047038‑2047228
                                                                                                       |                                                                                               
cggCGTGGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCa                                                                                                  <  2:4445970/99‑1 (MQ=255)
 aGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.g                                                                                                <  2:4629654/101‑1 (MQ=255)
    gTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcg                                                                                             >  2:1286472/1‑98 (MQ=255)
     ttGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcgc                                                                                            <  2:5328285/101‑5 (MQ=255)
      tGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcgcg                                                                                           <  2:5705395/101‑6 (MQ=255)
        ggCTGCCCAGGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGtt                                                                                          <  2:2377278/101‑1 (MQ=255)
         tcTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                                                                         <  2:2650517/100‑1 (MQ=255)
         gcaacccATAAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                                                                         <  1:1670055/96‑1 (MQ=255)
         gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                                                                         <  2:3309932/101‑1 (MQ=255)
         gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                                                                         >  2:5424903/1‑101 (MQ=255)
          ctggacATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTg                                                                                        >  2:4902545/6‑101 (MQ=255)
          cTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTg                                                                                        >  2:294091/1‑101 (MQ=255)
           tgcccATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTgg                                                                                       <  2:3131519/98‑1 (MQ=255)
            gACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTggg                                                                                      <  1:3311780/101‑1 (MQ=255)
              ccATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGcc                                                                                    >  1:602241/1‑101 (MQ=255)
               cATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCa                                                                                   >  1:5173739/1‑101 (MQ=255)
               cATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCa                                                                                   >  2:2836393/1‑101 (MQ=255)
                 tGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACCCC.CGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaaa                                                                                 >  2:3594752/1‑101 (MQ=255)
                 tGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaaa                                                                                 >  1:5273107/1‑101 (MQ=255)
                 tGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaaa                                                                                 >  2:4739318/1‑101 (MQ=255)
                   acccAGCCGCTGGCCGCCTCCGAGCGGG.CCNA.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAAtt                                                                               <  1:3929570/99‑1 (MQ=255)
                    aCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCGAAAttt                                                                              >  1:963350/1‑101 (MQ=255)
                     ccAGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTg                                                                             <  1:5394433/101‑1 (MQ=255)
                       aGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGca                                                                           >  2:2332936/1‑101 (MQ=255)
                       aGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGca                                                                           <  2:3221365/101‑1 (MQ=255)
                       aGCCGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGca                                                                           >  2:3892135/1‑101 (MQ=255)
                         ccGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGcaca                                                                         >  1:1995772/1‑101 (MQ=255)
                          cGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCGCaa                                                                        <  2:616800/101‑1 (MQ=255)
                          cGCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACaa                                                                        >  2:562004/1‑101 (MQ=255)
                           gCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                                                                       <  2:2705760/101‑1 (MQ=255)
                           gCTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                                                                       <  1:1206247/101‑1 (MQ=255)
                            cTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAgg                                                                      <  2:4959448/101‑1 (MQ=255)
                            cTGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAgg                                                                      <  1:1613476/101‑1 (MQ=255)
                             tGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                                                                     >  1:1755287/1‑101 (MQ=255)
                             tGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                                                                     <  1:5058872/101‑1 (MQ=255)
                             tGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                                                                     >  1:2056849/1‑101 (MQ=255)
                             tGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                                                                     <  1:3611336/101‑1 (MQ=255)
                             tGGCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                                                                     <  1:225080/101‑1 (MQ=255)
                              ggCCGCCTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                                                                    >  1:3546741/1‑101 (MQ=255)
                               gccgccTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGttt                                                                   >  1:4370999/1‑101 (MQ=255)
                                ccgccTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                                                                  <  2:3979808/101‑1 (MQ=255)
                                   ccTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGc                                                               <  1:1535564/101‑1 (MQ=255)
                                    cTCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCa                                                              >  2:3078220/1‑101 (MQ=255)
                                     tCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCag                                                             <  1:2991528/101‑1 (MQ=255)
                                     tCCGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCag                                                             <  1:2836554/101‑1 (MQ=255)
                                      ccGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga                                                            <  2:3577611/101‑1 (MQ=255)
                                      ccGAGCGCGCCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga                                                            <  1:1286555/101‑1 (MQ=255)
                                          gcgcgcCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaa                                                        <  2:4346876/101‑1 (MQ=255)
                                          gcgcgcCC.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaa                                                        <  1:2284636/101‑1 (MQ=255)
                                            gcgccc.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAg                                                      <  1:2509206/97‑1 (MQ=255)
                                             cgccc.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgg                                                     >  1:257661/4‑101 (MQ=255)
                                                cc.A.CCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg                                                  <  1:1384121/101‑1 (MQ=255)
                                                    accgccgggttGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGAc                                                <  2:2200652/91‑1 (MQ=255)
                                                       gccgggttGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGAt                                             <  1:4457269/94‑1 (MQ=255)
                                                        ccgggttGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATg                                            <  2:2516134/95‑1 (MQ=255)
                                                        ccgggttGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATg                                            >  1:3511503/7‑101 (MQ=255)
                                                         cgggttGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGc                                           >  2:519499/6‑101 (MQ=255)
                                                          gggttGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATgag                                          >  2:1601566/5‑99 (MQ=255)
                                                            gttGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGcccc                                        <  1:5585571/99‑1 (MQ=255)
                                                            gttGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGcccc                                        >  1:4205181/3‑101 (MQ=255)
                                                            gttGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGcccc                                        <  1:2144634/99‑1 (MQ=255)
                                                              tGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCgg                                      <  2:1356567/101‑1 (MQ=255)
                                                              tGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCgg                                      >  1:4379212/1‑101 (MQ=255)
                                                              tGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCgg                                      >  1:4548311/1‑101 (MQ=255)
                                                               ggAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGc                                     <  2:2125931/101‑1 (MQ=255)
                                                               ggAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGc                                     <  1:2123740/101‑1 (MQ=255)
                                                                gagaTGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCt                                    >  2:1209425/1‑101 (MQ=255)
                                                                gagaTGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCt                                    >  1:914421/1‑101 (MQ=255)
                                                                  gaTGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTg                                  >  2:5148106/1‑101 (MQ=255)
                                                                  gaTGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTg                                  <  2:914345/101‑1 (MQ=255)
                                                                   aTGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGc                                 >  1:3185315/1‑101 (MQ=255)
                                                                   aTGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGc                                 <  1:4099187/101‑1 (MQ=255)
                                                                    tGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCg                                <  1:2185671/101‑1 (MQ=255)
                                                                    tGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCg                                >  1:781667/1‑101 (MQ=255)
                                                                        gACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCt                            >  1:5686348/1‑101 (MQ=255)
                                                                        gACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCt                            >  1:373264/1‑101 (MQ=255)
                                                                          cATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGt                          <  1:2688470/101‑1 (MQ=255)
                                                                           aTACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGtt                         >  2:2273939/1‑101 (MQ=255)
                                                                           aTACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGtt                         >  2:3465696/1‑101 (MQ=255)
                                                                           aTACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGtt                         <  1:3783550/101‑1 (MQ=255)
                                                                            tACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGttt                        >  1:170982/1‑101 (MQ=255)
                                                                              ctctATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCg                      <  2:2046739/101‑1 (MQ=255)
                                                                                ctATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCggg                    <  1:179380/101‑1 (MQ=255)
                                                                                ctATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCggg                    >  2:4263870/1‑101 (MQ=255)
                                                                                 tATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGa                   >  1:4852233/1‑101 (MQ=255)
                                                                                 ctttctccctcAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGa                   <  1:4001430/94‑1 (MQ=255)
                                                                                  aTTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGaa                  <  2:3753678/101‑1 (MQ=255)
                                                                                   ttctccctcAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGaaa                 >  1:4303979/6‑101 (MQ=255)
                                                                                   ttctccctcAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGaaa                 >  1:2900519/6‑101 (MQ=255)
                                                                                      tccctcAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAAttt              >  1:1567889/3‑101 (MQ=255)
                                                                                      tccctcAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAAttt              <  2:716610/99‑1 (MQ=255)
                                                                                         ctcAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTccc           <  1:3078394/101‑1 (MQ=255)
                                                                                          tcAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTCCcg          >  1:1014896/1‑101 (MQ=255)
                                                                                          tcAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTCCcg          <  1:2404945/101‑1 (MQ=255)
                                                                                          tcAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTCCcg          >  1:4888215/1‑101 (MQ=255)
                                                                                          tcAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTCCcg          >  2:2622314/1‑101 (MQ=255)
                                                                                            agcgcgtctcAGGCGCGTTTGGGCCAAATTGGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTCCcgcg        <  2:1755188/95‑1 (MQ=255)
                                                                                               gcgtctcAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTCCCGCGAtt     <  1:2367936/98‑1 (MQ=255)
                                                                                               gcgtctcAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTCCCGCGAtt     <  1:651372/98‑1 (MQ=255)
                                                                                                 gtctcAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTCCCGCGCTTGc   >  1:1124470/2‑101 (MQ=255)
                                                                                                       |                                                                                               
CAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CC.A.CCG.CGGG.TGGAGATGCCGACATACTCTATTCT.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTCCCGCGATTGCC  >  NC_014034/2047038‑2047228

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG