Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,057,825 +C 81.8% intergenic (‑591/‑311) RCAP_rcc01900 ← / → RCAP_rcc01901 hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,057,8251.→C81.8% 107.1 ‑0.2 ‑0.3181intergenic (‑591/‑311)RCAP_rcc01900/RCAP_rcc01901hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (67/81):  ref base (16/17):  total (83/98)
Fisher's exact test strand distribution p-value = 8.47e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.76e-01

Only 100 of 214 total aligned reads displayed.

AAAATTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.CGCG.CG.C..A.GC.G.TT.CCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAA  >  NC_014034/2057725‑2057911
                                                                                                     |                                                                                                
aaaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAt                                                                                                   >  1:1480496/1‑101 (MQ=255)
aaaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAt                                                                                                   <  2:5389761/101‑1 (MQ=255)
 aaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.c                                                                                                 >  1:4632077/1‑101 (MQ=255)
 aaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.c                                                                                                 >  1:1988886/1‑101 (MQ=255)
   aTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.c.cc                                                                                              <  1:3816730/101‑2 (MQ=255)
   aTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.c.cc                                                                                              <  2:4635947/101‑2 (MQ=255)
     ttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.c.ccgc                                                                                            <  1:772683/101‑4 (MQ=255)
     ttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.c.ccgc                                                                                            >  1:2858733/1‑98 (MQ=255)
     ttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.c.ccgc                                                                                            <  1:5380863/101‑4 (MQ=255)
     ttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.c.ccgc                                                                                            >  1:2956878/1‑98 (MQ=255)
      tGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.c.ccgcg                                                                                           >  2:1515051/1‑97 (MQ=255)
       gTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.c.cg.cg.c                                                                                       >  1:4727346/1‑101 (MQ=255)
         ccTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.c.cg.cg.ccg                                                                                     <  1:5596783/101‑3 (MQ=255)
          cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gc                                                                                 >  1:658477/1‑101 (MQ=255)
          cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gc                                                                                 <  2:4924707/101‑1 (MQ=255)
           tGTTTACCGCCCATTAGCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gca                                                                                <  1:825080/101‑2 (MQ=255)
           tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gca                                                                                <  2:3612525/101‑2 (MQ=255)
           tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gca                                                                                >  1:3859925/1‑100 (MQ=255)
            gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gcaa                                                                               >  2:4750152/1‑99 (MQ=255)
             tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gcaag                                                                              >  1:5190561/1‑98 (MQ=255)
             tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gcaag                                                                              >  2:2365727/1‑98 (MQ=255)
               tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gcaagcg                                                                            >  1:2294712/1‑96 (MQ=255)
               tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gcaagcg                                                                            <  2:627293/101‑6 (MQ=255)
                  cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gcaagcgttt                                                                         <  2:3079999/101‑9 (MQ=255)
                   gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gcaagcgtttc                                                                        <  2:3218194/101‑10 (MQ=255)
                   gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gcaagcgtttc                                                                        >  1:3004071/1‑92 (MQ=255)
                    cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gcaagcgtttcc                                                                       >  1:46918/1‑91 (MQ=255)
                    cccATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.CG.C..C.Gcaagcgtttcc                                                                       <  1:201301/101‑11 (MQ=255)
                            cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGc                                                                 <  2:4288153/101‑1 (MQ=255)
                            cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGc                                                                 >  2:4443201/1‑101 (MQ=255)
                            cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGc                                                                 <  1:5738032/101‑1 (MQ=255)
                             ccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCa                                                                >  2:352109/1‑101 (MQ=255)
                             ccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCa                                                                <  1:2719473/101‑1 (MQ=255)
                             ccTGCTTCGGCGCGCGGACTCCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCa                                                                >  2:1929864/1‑101 (MQ=255)
                              cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCAt                                                               <  1:2531174/101‑1 (MQ=255)
                                gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCa                                                             <  1:4513587/101‑1 (MQ=255)
                                  ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCatt                                                           <  1:1029305/101‑3 (MQ=255)
                                   tCGGCGCGCCGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTc                                                          <  2:3491940/101‑1 (MQ=255)
                                    cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCt                                                         <  1:5218055/101‑1 (MQ=255)
                                    cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCt                                                         <  2:3859683/101‑1 (MQ=255)
                                    cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCt                                                         <  2:3016835/101‑1 (MQ=255)
                                     ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCtt                                                        >  2:2260781/1‑101 (MQ=255)
                                      gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTg                                                       >  2:5327472/1‑101 (MQ=255)
                                       cgcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTgg                                                      <  2:1569549/101‑1 (MQ=255)
                                       cgcgcgGACTTCGGGGCAGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTgg                                                      <  1:2830365/101‑1 (MQ=255)
                                         cgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAAGCGGCCTCATTCTTGG.cc                                                   >  2:816395/1‑101 (MQ=255)
                                         cgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGG.cc                                                   >  2:1236921/1‑101 (MQ=255)
                                          gcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGG.ccc                                                  >  1:2675772/1‑101 (MQ=255)
                                           ccgACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGG.cccc                                                 <  2:1024579/99‑1 (MQ=255)
                                            ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGG.ccccc                                                <  1:218588/101‑1 (MQ=255)
                                                ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGG.ccccccgcc                                            >  1:1266846/1‑97 (MQ=255)
                                                ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTAGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGG.ccccccgcc                                            <  1:5293479/101‑5 (MQ=255)
                                                  cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCgg                                           >  2:4593275/1‑101 (MQ=255)
                                                  cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCgg                                           <  2:5190275/101‑1 (MQ=255)
                                                   ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGGGACAATCC.CGCGCCG.C..ACGC.G.TTCCCGAAGCGGCATCATTCTTGGCCCCCCGCCggc                                          >  1:492470/1‑100 (MQ=255)
                                                   ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCggg                                          <  2:1408739/101‑1 (MQ=255)
                                                   ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCggg                                          >  1:4182102/1‑101 (MQ=255)
                                                    gggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGa                                         <  1:1527293/101‑1 (MQ=255)
                                                    gggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGa                                         <  2:5455272/101‑1 (MQ=255)
                                                     ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGAc                                        <  2:5239790/101‑1 (MQ=255)
                                                     ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGAc                                        <  1:2148911/101‑1 (MQ=255)
                                                       cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCTCCGGGACaa                                      >  1:3448384/1‑101 (MQ=255)
                                                       cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACaa                                      >  2:1582178/1‑101 (MQ=255)
                                                       cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACaa                                      <  2:1309344/101‑1 (MQ=255)
                                                        ggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAAt                                     <  2:2631554/101‑1 (MQ=255)
                                                         gcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCCCCGGGACAATc                                    >  1:1604680/1‑101 (MQ=255)
                                                          cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                   <  2:2835536/101‑1 (MQ=255)
                                                          cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GCTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                   <  2:2956711/101‑1 (MQ=255)
                                                           ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                  <  2:895399/101‑1 (MQ=255)
                                                           ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                  >  2:272798/1‑101 (MQ=255)
                                                           ggcggAGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                  >  2:4237504/1‑101 (MQ=255)
                                                            gcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACg                                 <  2:3159164/101‑1 (MQ=255)
                                                               gggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgcc                              <  2:3291773/101‑1 (MQ=255)
                                                                ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                             >  2:1286767/1‑101 (MQ=255)
                                                                 gCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccgc                            >  1:2247106/1‑101 (MQ=255)
                                                                  ctctTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccaga                           >  1:3588513/1‑98 (MQ=255)
                                                                    ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                         <  2:668862/101‑1 (MQ=255)
                                                                      tGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                       <  1:2337936/101‑1 (MQ=255)
                                                                      tGCGATCCTGCGTTTCCGCATTGGCGACAAG.CTCGCGCCGCC..A.GC.GTTT.CCTAACCGCCATCGTTCCTGGCCCCCCGCCGGGAGAAATCCGTCCCCCTCAt                       >  2:4059051/1‑101 (MQ=255)
                                                                      tGCGATCCTGCGTTGCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                       <  2:4855519/101‑1 (MQ=255)
                                                                       gCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAtt                      <  2:5703768/101‑1 (MQ=255)
                                                                        cGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                     <  2:724736/101‑1 (MQ=255)
                                                                           tCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                  >  2:3699626/1‑101 (MQ=255)
                                                                            ccTGCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                 <  1:80029/101‑1 (MQ=255)
                                                                               gCGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc              <  2:3744794/101‑1 (MQ=255)
                                                                                cGTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa             >  2:1786367/1‑101 (MQ=255)
                                                                                 gTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt            >  2:1791683/1‑101 (MQ=255)
                                                                                 gTTTCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt            <  1:3765219/101‑1 (MQ=255)
                                                                                  tttCCGGGTTGGCGACAATCC.CGCG.CC.CCAA.GCTT.TT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt           <  1:1739980/101‑1 (MQ=255)
                                                                                  tttCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt           <  1:4750433/101‑1 (MQ=255)
                                                                                   ttCCGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg          <  2:3910792/101‑1 (MQ=255)
                                                                                     ccGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg        >  1:67080/1‑101 (MQ=255)
                                                                                     ccGGATTGGCCACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCTGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg        <  1:4237764/101‑1 (MQ=255)
                                                                                      cGGATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGa       >  1:3907494/1‑101 (MQ=255)
                                                                                       ggATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCgct      >  2:5764902/1‑99 (MQ=255)
                                                                                       ggATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt      >  1:328837/1‑101 (MQ=255)
                                                                                       ggATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt      <  1:1393894/101‑1 (MQ=255)
                                                                                        gATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc     <  1:913131/101‑1 (MQ=255)
                                                                                        gATTGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc     >  2:1155425/1‑101 (MQ=255)
                                                                                           tGGCGACAATCC.CGCGCCG.CA.A.GC.GTTT.CCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa  <  1:1896036/101‑1 (MQ=255)
                                                                                                     |                                                                                                
AAAATTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.CGCG.CG.C..A.GC.G.TT.CCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAA  >  NC_014034/2057725‑2057911

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG