Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,135,154 +C 100% intergenic (‑148/+63) RCAP_rcc01974 ← / ← RCAP_rcc01975 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,135,1541.→C100.0% 1004.7 267intergenic (‑148/+63)RCAP_rcc01974/RCAP_rcc01975hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (119/147):  ref base (1/0):  total (120/147)

Only 100 of 300 total aligned reads displayed.

ACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.GG.GCCAAAGTTCAAAGG.CCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGACAC  >  NC_014034/2135055‑2135249
                                                                                                      |                                                                                               
aCTTATGGTCAATCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAgg                                                                                                  <  2:2562317/101‑1 (MQ=255)
   tATGTTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGG.ccc                                                                                              <  2:3328472/101‑1 (MQ=255)
   tATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGG.ccc                                                                                              >  2:1403480/1‑101 (MQ=255)
    ttGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGG.cccc                                                                                             <  2:3736731/100‑2 (MQ=255)
    agggTAAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGG.cccc                                                                                             <  1:2166899/99‑2 (MQ=255)
    aTGGTAAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGG.cccc                                                                                             <  2:5496792/101‑2 (MQ=255)
     tGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGG.ccccg                                                                                            <  1:1647487/101‑3 (MQ=255)
      ggTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGG.ccccgc                                                                                           >  1:1261973/1‑98 (MQ=255)
      ggTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGG.ccccgc                                                                                           <  2:2310638/101‑4 (MQ=255)
          aaGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTc                                                                                        >  1:1307155/1‑101 (MQ=255)
          aaGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTc                                                                                        >  2:2579347/1‑101 (MQ=255)
            gCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCa                                                                                      <  2:1488558/101‑1 (MQ=255)
            gCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCa                                                                                      <  1:2736882/101‑1 (MQ=255)
            gCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCa                                                                                      <  2:2850726/101‑1 (MQ=255)
            gCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCa                                                                                      >  1:2103799/1‑101 (MQ=255)
             ccATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAg                                                                                     >  1:2907355/1‑101 (MQ=255)
             ccATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAg                                                                                     <  2:529108/101‑1 (MQ=255)
             ccATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAg                                                                                     <  1:1090274/101‑1 (MQ=255)
                ttAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCgg                                                                                  <  1:2784290/101‑1 (MQ=255)
                  aaaTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCgggg                                                                                >  2:3035971/1‑101 (MQ=255)
                  aaaTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCgggg                                                                                >  1:1753950/1‑101 (MQ=255)
                  aaaTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCgggg                                                                                >  2:5736776/1‑101 (MQ=255)
                  aaaTTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCgggg                                                                                >  1:540881/1‑101 (MQ=255)
                     ttGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCa                                                                             <  1:1475077/101‑1 (MQ=255)
                      tGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCaa                                                                            <  1:2242704/101‑1 (MQ=255)
                      tGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCaa                                                                            >  2:3005853/1‑101 (MQ=255)
                        cGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAgg                                                                          <  1:1540065/101‑1 (MQ=255)
                         gTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAggg                                                                         >  2:4633997/1‑101 (MQ=255)
                         gTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAggg                                                                         >  2:934951/1‑101 (MQ=255)
                           ttATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGc                                                                       <  1:5653772/101‑1 (MQ=255)
                            tATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGcg                                                                      >  1:5105110/1‑101 (MQ=255)
                             aTCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGcga                                                                     >  1:5274373/1‑101 (MQ=255)
                             aTCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGcga                                                                     >  1:4531758/1‑101 (MQ=255)
                              tCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGcgac                                                                    <  1:5195801/101‑1 (MQ=255)
                                gCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGcgacga                                                                  >  2:1539915/1‑101 (MQ=255)
                                 cAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCgacgac                                                                 >  2:4563046/1‑101 (MQ=255)
                                   aCTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACgg                                                               <  2:3190470/101‑1 (MQ=255)
                                    cTTTTCTTCCGCGTCGATTCGTGGAAGCTTTGACACGCGGGCTTTGAGGG.GCCAAAGTCCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGt                                                              <  1:235143/101‑1 (MQ=255)
                                     tttGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTg                                                             >  2:2325470/1‑101 (MQ=255)
                                     tttGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTg                                                             <  1:4228223/101‑1 (MQ=255)
                                      ttGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGa                                                            >  2:4183693/1‑101 (MQ=255)
                                       tGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGaa                                                           >  1:762232/1‑101 (MQ=255)
                                       tGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGaa                                                           >  2:3481190/1‑101 (MQ=255)
                                        gCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAg                                                          >  2:1842033/1‑101 (MQ=255)
                                        gCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAg                                                          >  2:2267510/1‑101 (MQ=255)
                                        gCTTCCGCGTCCATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAg                                                          <  2:2416662/101‑1 (MQ=255)
                                        gCTTCCGCGTAGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAg                                                          <  2:1320992/101‑1 (MQ=255)
                                         cTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGGGAAgg                                                         >  1:1354211/1‑101 (MQ=255)
                                          ttCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGc                                                        <  1:246632/101‑1 (MQ=255)
                                          ttCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGc                                                        <  1:913326/101‑1 (MQ=255)
                                          ttCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGc                                                        >  2:5000461/1‑101 (MQ=255)
                                          ttCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGc                                                        >  1:2532591/1‑101 (MQ=255)
                                           tCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCa                                                       <  2:1111642/101‑1 (MQ=255)
                                           tCCGCGGCGATTCGTGTAAGCTTCGACACGCGGTCTTTGA.GGTGCCAAAGTCCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCa                                                       <  2:5665491/101‑1 (MQ=255)
                                            ccGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCaa                                                      <  1:3481407/101‑1 (MQ=255)
                                             cgcgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCaaa                                                     >  1:167660/1‑101 (MQ=255)
                                             cgcgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCaaa                                                     <  1:3700195/101‑1 (MQ=255)
                                              gcgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAg                                                    <  2:3109860/101‑1 (MQ=255)
                                               cgTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGCGAAGGCAAAGc                                                   >  1:2043663/1‑101 (MQ=255)
                                                gTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGGGAAGGCAAAGCa                                                  >  1:1910461/1‑101 (MQ=255)
                                                  cGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAAc                                                >  1:594007/1‑101 (MQ=255)
                                                  cGATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAAc                                                <  1:3520245/101‑1 (MQ=255)
                                                   gATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACt                                               >  2:4052727/1‑101 (MQ=255)
                                                   gATTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACt                                               >  1:391402/1‑101 (MQ=255)
                                                    aTTCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTg                                              >  2:3644613/1‑101 (MQ=255)
                                                      tCGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTc                                            <  1:3036145/101‑1 (MQ=255)
                                                       cGTGGAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCg                                           <  1:1789365/101‑1 (MQ=255)
                                                          ggAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTa                                        <  2:4103892/101‑1 (MQ=255)
                                                          ggAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTa                                        <  2:2087345/101‑1 (MQ=255)
                                                          ggAAGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTa                                        >  2:2598176/1‑101 (MQ=255)
                                                            aaGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACt                                      <  1:5012414/101‑1 (MQ=255)
                                                             aGCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTg                                     <  1:4718205/101‑1 (MQ=255)
                                                              gCTTCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGa                                    <  2:2180065/101‑1 (MQ=255)
                                                                ttCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATc                                  <  2:540831/101‑1 (MQ=255)
                                                                 tCGACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCt                                 <  1:3391710/101‑1 (MQ=255)
                                                                   gACACGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAg                               >  1:4375808/1‑101 (MQ=255)
                                                                    acacGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGa                              <  1:2674469/101‑1 (MQ=255)
                                                                      acGCGGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGata                            >  2:5194252/1‑101 (MQ=255)
                                                                       cgcgGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGatat                           <  1:2598318/101‑1 (MQ=255)
                                                                        gcgGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGAtata                          <  2:4822019/101‑1 (MQ=255)
                                                                         cgGGCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATaa                         <  2:601219/101‑1 (MQ=255)
                                                                          gggCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAAt                        >  1:624554/1‑101 (MQ=255)
                                                                          gggCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAAt                        <  1:3006859/101‑1 (MQ=255)
                                                                            gCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTc                      >  2:3544317/1‑101 (MQ=255)
                                                                            gCTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTc                      <  1:4495587/101‑1 (MQ=255)
                                                                             cTTTGAGGG.GCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTcc                     >  2:1695089/1‑101 (MQ=255)
                                                                                tgaggg.gCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGcc                  <  1:5768126/97‑1 (MQ=255)
                                                                                tgaggg.gCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGcc                  >  1:3801629/5‑101 (MQ=255)
                                                                                 gaggg.gCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCa                 >  2:4359006/4‑101 (MQ=255)
                                                                                 gaggg.gCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCa                 <  1:4577963/98‑1 (MQ=255)
                                                                                  aggg.gCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCaa                >  1:1173541/3‑101 (MQ=255)
                                                                                   ggg.gCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAAt               >  2:4432206/2‑101 (MQ=255)
                                                                                    gg.gCCAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATg              <  2:1188817/101‑1 (MQ=255)
                                                                                        ccAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTg           <  2:380379/101‑1 (MQ=255)
                                                                                        ccAAAGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTg           <  2:1653332/101‑1 (MQ=255)
                                                                                           aaGTTCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTc        <  1:5500485/101‑1 (MQ=255)
                                                                                              ttCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGa     >  1:4439758/1‑101 (MQ=255)
                                                                                               tCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGac    >  2:4701777/1‑101 (MQ=255)
                                                                                               tCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGac    <  2:4318046/101‑1 (MQ=255)
                                                                                               tCAAAGGCCCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGac    <  1:4124857/101‑1 (MQ=255)
                                                                                                      |                                                                                               
ACTTATGGTCAAGCCATTAAATTGCGTTTATCGCAACTTTGCTTCCGCGTCGATTCGTGGAAGCTTCGACACGCGGGCTTTGA.GG.GCCAAAGTTCAAAGG.CCCGCTTTCCAGCGGGGCCAAGGGGCGACGACGGTGAAGGCAAAGCAACTGTCGCTACTGATCTAGATATAATTCCGCCAATGTTGCTCGGACAC  >  NC_014034/2135055‑2135249

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG