Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,313 +A 100% coding (64/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3131.→A100.0% 932.2 233coding (64/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (124/109):  ref base (0/0):  total (124/109)

Only 100 of 264 total aligned reads displayed.

CAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..GG.GTTG..GTCGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGC  >  NC_014034/2177218‑2177406
                                                                                                        |                                                                                                
cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCg                                                                                                   >  2:4480119/1‑101 (MQ=255)
cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCg                                                                                                   >  1:5436352/1‑101 (MQ=255)
   ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aag                                                                                               <  1:801130/101‑3 (MQ=255)
     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagcc                                                                                             <  1:5116068/101‑5 (MQ=255)
     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagcc                                                                                             <  1:2574211/101‑5 (MQ=255)
     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGCCTTGGTGAT.CAGACAGGCCCCGTTTTCTTCCG.aagcc                                                                                             <  2:534336/101‑5 (MQ=255)
      cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagccg                                                                                            <  2:223261/101‑6 (MQ=255)
      cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagccc                                                                                            >  2:5720008/1‑96 (MQ=255)
        tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                                                                           >  1:1883047/1‑101 (MQ=255)
        tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                                                                           >  1:1391040/1‑101 (MQ=255)
        tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                                                                           >  2:3222941/1‑101 (MQ=255)
         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                                                          >  2:1477988/1‑101 (MQ=255)
         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                                                          <  2:773897/101‑1 (MQ=255)
          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                                                                         <  2:2020097/101‑1 (MQ=255)
          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                                                                         >  1:2613518/1‑101 (MQ=255)
           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                                                                        >  1:2071670/1‑101 (MQ=255)
           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                                                                        <  2:3479941/101‑1 (MQ=255)
           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                                                                        >  1:311254/1‑101 (MQ=255)
             gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGc                                                                                      <  2:4837739/101‑1 (MQ=255)
               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                                                    >  1:5416893/1‑101 (MQ=255)
               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                                                    <  2:917580/101‑1 (MQ=255)
                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                                                   >  2:763129/1‑101 (MQ=255)
                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGACCCCGGTTTAGGCCGAAGCCGCCCCGGCCAg                                                                                   >  1:649468/1‑101 (MQ=255)
                  cTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGACCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                                                                                 >  1:5569997/1‑101 (MQ=255)
                      gCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                                                                             >  2:5558721/1‑101 (MQ=255)
                        cGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                                                           >  2:2468312/1‑101 (MQ=255)
                        cGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                                                           <  2:3570228/101‑1 (MQ=255)
                        cGAAGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                                                           >  1:459089/1‑101 (MQ=255)
                          aaGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                                                         >  1:4092636/1‑101 (MQ=255)
                           aGCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGTCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACg                                                                        <  2:4016098/101‑1 (MQ=255)
                            gCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                                                       <  2:311221/101‑1 (MQ=255)
                            gCACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                                                       >  1:193790/1‑101 (MQ=255)
                             cACCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                                                                      <  2:1092461/101‑1 (MQ=255)
                              aCCTTCCTTCATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                     >  1:3333928/1‑101 (MQ=255)
                                cttccttcATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                  <  2:5159147/101‑1 (MQ=255)
                                cttccttcATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                  <  2:1114196/101‑1 (MQ=255)
                                cttccttcATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                  <  1:5748221/101‑1 (MQ=255)
                                cttccttcATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                  >  1:2440965/1‑101 (MQ=255)
                                 ttccttcATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                                                 >  1:4793481/1‑100 (MQ=255)
                                  tccttcATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                                <  1:3637731/101‑3 (MQ=255)
                                  tccttcATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                                >  2:5517127/1‑99 (MQ=255)
                                   ccttcATCTTGACGAAAAA..GGCGTTGGTGTCGGTCTT.GGGGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                                               <  1:1633277/101‑4 (MQ=255)
                                    cttcATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                                              <  2:3779468/101‑5 (MQ=255)
                                      tcATCTTGACG.AAAAAGNG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                             <  2:2783131/101‑1 (MQ=255)
                                       cATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                            >  2:1250857/1‑101 (MQ=255)
                                       cATCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                            >  2:2671445/1‑101 (MQ=255)
                                        aTCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                                                          >  2:2590773/1‑101 (MQ=255)
                                         tCTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                                                         >  2:3370712/1‑101 (MQ=255)
                                          cTTGACG.AAAAAGGG.GTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                                                        <  2:4588846/101‑1 (MQ=255)
                                                       aggggttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                                              <  1:41403/91‑1 (MQ=255)
                                                        ggggttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa                                             <  1:2213477/92‑1 (MQ=255)
                                                         gggttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAg                                            >  1:4413946/9‑101 (MQ=255)
                                                           gttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt                                         <  1:4852411/95‑1 (MQ=255)
                                                           gttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt                                         >  1:601785/7‑101 (MQ=255)
                                                            ttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt                                        <  1:3223141/96‑2 (MQ=255)
                                                            tggggttcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt                                        <  2:3064368/95‑2 (MQ=255)
                                                             tggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAATGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                       >  1:4929047/5‑99 (MQ=255)
                                                              ggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                      >  1:2078100/4‑98 (MQ=255)
                                                              ggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                      <  2:4188460/98‑4 (MQ=255)
                                                              ggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                      >  1:5344879/4‑98 (MQ=255)
                                                              ggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                      >  1:5228088/4‑98 (MQ=255)
                                                              ggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                      >  2:368603/4‑98 (MQ=255)
                                                               gtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg                                     <  1:3671023/99‑5 (MQ=255)
                                                               gtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg                                     <  1:3976571/99‑5 (MQ=255)
                                                                tgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc                                    <  1:1725660/100‑6 (MQ=255)
                                                                tgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc                                    <  1:375980/100‑6 (MQ=255)
                                                                 gtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                    >  2:424126/1‑101 (MQ=255)
                                                                 gtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                    >  2:2844219/1‑101 (MQ=255)
                                                                 gtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                    >  1:1279570/1‑101 (MQ=255)
                                                                  tcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcat                                   <  1:915617/101‑1 (MQ=255)
                                                                  tcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgagcat                                  >  1:2804273/1‑94 (MQ=255)
                                                                        cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                              <  2:5422014/92‑1 (MQ=255)
                                                                         ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                             <  2:241180/93‑1 (MQ=255)
                                                                         ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                             <  1:3921805/93‑1 (MQ=255)
                                                                         ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                             >  2:3712874/9‑101 (MQ=255)
                                                                          tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                            >  1:4323030/8‑101 (MQ=255)
                                                                            gtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGt                          >  1:236244/6‑101 (MQ=255)
                                                                               atcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctc                       >  1:5164820/3‑101 (MQ=255)
                                                                                tcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctct                      >  1:1706364/2‑101 (MQ=255)
                                                                                  agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcc                    >  1:4689087/1‑101 (MQ=255)
                                                                                  agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcc                    >  2:2960200/1‑101 (MQ=255)
                                                                                   gagagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcct                   <  2:2628246/98‑1 (MQ=255)
                                                                                    acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcctc                  >  1:1037473/1‑101 (MQ=255)
                                                                                    acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcctc                  >  2:3590806/1‑101 (MQ=255)
                                                                                    acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCGctcctc                  >  2:5275851/1‑101 (MQ=255)
                                                                                     cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                 >  2:1754358/1‑101 (MQ=255)
                                                                                      agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                <  1:324564/101‑1 (MQ=255)
                                                                                      agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                <  1:2498604/101‑1 (MQ=255)
                                                                                        gCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGt              <  2:4687457/101‑1 (MQ=255)
                                                                                        gCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGt              <  1:113793/101‑1 (MQ=255)
                                                                                         ccccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGtt             >  2:3973548/1‑101 (MQ=255)
                                                                                          cccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt            >  2:1809489/1‑101 (MQ=255)
                                                                                          cccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt            <  2:1390952/101‑1 (MQ=255)
                                                                                          cccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt            <  1:4510750/101‑1 (MQ=255)
                                                                                           ccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc           >  2:3031186/1‑101 (MQ=255)
                                                                                              gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt        >  2:1564857/1‑101 (MQ=255)
                                                                                              gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt        <  2:3830431/101‑1 (MQ=255)
                                                                                               tttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg       >  2:2136670/1‑101 (MQ=255)
                                                                                                   ggCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg   >  2:49115/1‑101 (MQ=255)
                                                                                                   ggCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg   >  1:4713726/1‑101 (MQ=255)
                                                                                                        |                                                                                                
CAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..GG.GTTG..GTCGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGC  >  NC_014034/2177218‑2177406

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG