Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,342 +G 100% coding (35/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3421.→G100.0% 843.2 220coding (35/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (110/110):  ref base (0/0):  total (110/110)

Only 100 of 253 total aligned reads displayed.

ACCTTCCTTCATCTTGACGAAAA..GGGTTG..G..TCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGA  >  NC_014034/2177248‑2177435
                                                                                                       |                                                                                               
  cttccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                                               <  2:5159147/101‑1 (MQ=255)
   ttccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                                                                              >  1:2384526/1‑100 (MQ=255)
    tccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGGTCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                                                             >  2:2951746/1‑99 (MQ=255)
    tccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                                                             >  2:5517127/1‑99 (MQ=255)
    tccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                                                             >  2:1461054/1‑99 (MQ=255)
    tccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                                                             <  1:3637731/101‑3 (MQ=255)
      cttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                                                                           <  2:3779468/101‑5 (MQ=255)
       ttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                                                          >  2:4013419/1‑96 (MQ=255)
        tcATCTTGACGAAAAAGNGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                                                          <  2:2783131/101‑1 (MQ=255)
        tcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                                                          <  2:2021450/101‑1 (MQ=255)
         cATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCTGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                                                         >  1:4823276/1‑101 (MQ=255)
         cATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                                                         >  2:2671445/1‑101 (MQ=255)
          aTCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                                                                                       >  2:2590773/1‑101 (MQ=255)
          aTCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                                                                                       >  1:594642/1‑101 (MQ=255)
           tCTTGACGAAAAGGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                                                                                      >  1:3133628/1‑101 (MQ=255)
                  aaaag..GGGTTG..GTTTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAgg                                                                              <  2:2632205/100‑1 (MQ=255)
                       aggggttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                                                                           <  1:41403/91‑1 (MQ=255)
                       aggggttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                                                                           >  1:1702003/11‑101 (MQ=255)
                       aggggttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                                                                           >  2:3579103/11‑101 (MQ=255)
                        ggggttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa                                                                          <  1:2213477/92‑1 (MQ=255)
                        ggggttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa                                                                          >  1:2073555/10‑101 (MQ=255)
                          ggttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t                                                                       >  2:1275394/8‑101 (MQ=255)
                             tggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAATGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                                                    >  1:4929047/5‑99 (MQ=255)
                             tggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                                                    >  2:2398772/5‑99 (MQ=255)
                             tggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                                                    >  1:4481657/5‑99 (MQ=255)
                              ggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                                                   <  2:4188460/98‑4 (MQ=255)
                              ggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                                                   >  2:3952694/4‑98 (MQ=255)
                              ggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                                                   >  1:5344879/4‑98 (MQ=255)
                              ggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                                                   >  1:5228088/4‑98 (MQ=255)
                              ggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                                                   <  2:3447890/98‑4 (MQ=255)
                              ggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                                                   >  1:2078100/4‑98 (MQ=255)
                              ggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                                                   <  2:649410/98‑4 (MQ=255)
                               gtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg                                                                  <  1:3671023/99‑5 (MQ=255)
                                tg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc                                                                 <  1:1725660/100‑6 (MQ=255)
                                 g..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                                                 >  2:424126/1‑101 (MQ=255)
                                 g..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                                                 >  2:2992303/1‑101 (MQ=255)
                                 g..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                                                 >  1:1279570/1‑101 (MQ=255)
                                 g..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                                                 >  2:2844219/1‑101 (MQ=255)
                                    tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcat                                                                >  2:367022/1‑101 (MQ=255)
                                    tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcat                                                                >  2:550471/1‑101 (MQ=255)
                                     cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                                               >  1:1369569/1‑101 (MQ=255)
                                        tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                                                            <  1:2978316/91‑1 (MQ=255)
                                         cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                           <  1:2598183/92‑1 (MQ=255)
                                          ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                          <  1:3588660/93‑1 (MQ=255)
                                          ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                          <  1:4703446/93‑1 (MQ=255)
                                          ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                          >  2:3712874/9‑101 (MQ=255)
                                          ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                          <  1:1936323/93‑1 (MQ=255)
                                          ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                          <  1:3921805/93‑1 (MQ=255)
                                           tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                                                         >  1:4323030/8‑101 (MQ=255)
                                               gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                     <  2:5416603/98‑1 (MQ=255)
                                                 tcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctct                                                   <  2:3133439/100‑1 (MQ=255)
                                                     acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGTGCCAGTTTGCGCATCATCACGGTCTctcctc                                               <  1:5169767/101‑1 (MQ=255)
                                                     acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcctc                                               >  2:4877728/1‑101 (MQ=255)
                                                     acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcctc                                               >  1:1037473/1‑101 (MQ=255)
                                                     acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCGctcctc                                               >  2:5275851/1‑101 (MQ=255)
                                                      cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                                              >  2:1754358/1‑101 (MQ=255)
                                                      cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                                              <  1:4645659/101‑1 (MQ=255)
                                                      cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                                              <  1:3709795/101‑1 (MQ=255)
                                                       agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                             >  1:3210544/1‑101 (MQ=255)
                                                       agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                             <  1:324564/101‑1 (MQ=255)
                                                       agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                             >  1:4764813/1‑101 (MQ=255)
                                                       agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                             <  1:2498604/101‑1 (MQ=255)
                                                         gCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGt                                           <  2:4687457/101‑1 (MQ=255)
                                                          ccccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGtt                                          <  1:4013666/101‑1 (MQ=255)
                                                            ccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                        >  2:3031186/1‑101 (MQ=255)
                                                             cGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                       >  2:4502769/1‑101 (MQ=255)
                                                               gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                     >  2:1564857/1‑101 (MQ=255)
                                                               gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                     >  1:624817/1‑101 (MQ=255)
                                                                tttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg                                    >  2:2136670/1‑101 (MQ=255)
                                                                   cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                 <  1:897021/101‑1 (MQ=255)
                                                                      ccgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcc                              <  2:4411483/97‑1 (MQ=255)
                                                                       cgaaGCCGCCCCGGCCAGCGCGGAGAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                             <  1:2543797/98‑1 (MQ=255)
                                                                       cgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                             >  2:246489/4‑101 (MQ=255)
                                                                       cgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                             <  1:5517419/98‑1 (MQ=255)
                                                                        gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                            >  2:605334/3‑101 (MQ=255)
                                                                        gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                            >  2:857208/3‑101 (MQ=255)
                                                                         aaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCACCAGGGCCAGCTTCCGCATCATTGCGGTCTCTCCTCCCGTTTCACTGGCGGCCcct                           <  2:1048968/100‑1 (MQ=255)
                                                                         aaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                           <  2:676588/100‑1 (MQ=255)
                                                                          aGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctc                          <  2:2319529/101‑1 (MQ=255)
                                                                           gccgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcc                         >  1:1093196/1‑101 (MQ=255)
                                                                             cgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCctcctc                       <  1:3049758/101‑1 (MQ=255)
                                                                              gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                      >  2:307788/1‑101 (MQ=255)
                                                                              gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                      >  1:2727838/1‑101 (MQ=255)
                                                                              gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                      >  2:4565075/1‑101 (MQ=255)
                                                                               ccccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCg                     <  1:763197/101‑1 (MQ=255)
                                                                                 ccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                   <  2:3238760/101‑1 (MQ=255)
                                                                                 ccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                   <  1:4360800/101‑1 (MQ=255)
                                                                                  cGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgggg                  >  1:3575406/1‑101 (MQ=255)
                                                                                   ggCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc                 <  1:1877466/101‑1 (MQ=255)
                                                                                    gCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                <  2:327960/101‑1 (MQ=255)
                                                                                     ccAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc               <  1:5150977/101‑1 (MQ=255)
                                                                                      cAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCg              >  2:5382026/1‑101 (MQ=255)
                                                                                       aGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCgg             >  2:338097/1‑101 (MQ=255)
                                                                                         cgcgGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGAc           <  2:1093121/101‑1 (MQ=255)
                                                                                            ggAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa        >  1:3396036/1‑101 (MQ=255)
                                                                                            ggAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa        <  2:594584/101‑1 (MQ=255)
                                                                                              aaaaCGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCGCCTCCTCCGGGGCTCGGACAAAGt      >  2:1899332/1‑101 (MQ=255)
                                                                                                 aCGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg   <  1:3299197/101‑1 (MQ=255)
                                                                                                 aCGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg   >  2:1185103/1‑101 (MQ=255)
                                                                                                 aCGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg   <  2:1882937/101‑1 (MQ=255)
                                                                                                       |                                                                                               
ACCTTCCTTCATCTTGACGAAAA..GGGTTG..G..TCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGA  >  NC_014034/2177248‑2177435

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG