Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,350 +C 100% coding (27/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3501.→C100.0% 891.4 234coding (27/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (118/115):  ref base (1/0):  total (119/115)

Only 100 of 252 total aligned reads displayed.

CATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGG.CCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCG  >  NC_014034/2177257‑2177444
                                                                                                     |                                                                                                
 aTCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                                                                                                 >  1:594642/1‑101 (MQ=255)
  tCTTGACGAAAAGGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                                                                                                >  1:3133628/1‑101 (MQ=255)
  tCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                                                                                                >  2:3370712/1‑101 (MQ=255)
   cTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                                                                                               <  1:2468446/101‑1 (MQ=255)
    ttGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cccc                                                                                              >  2:324520/1‑101 (MQ=255)
              aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.cc                                                                                    <  1:41403/91‑1 (MQ=255)
              aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.cc                                                                                    >  1:1702003/11‑101 (MQ=255)
              aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.cc                                                                                    >  2:3579103/11‑101 (MQ=255)
               ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCa                                                                                   >  1:2073555/10‑101 (MQ=255)
               ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCa                                                                                   <  1:2213477/92‑1 (MQ=255)
                 ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.t                                                                                >  2:1275394/8‑101 (MQ=255)
                  gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tt                                                                               >  2:2701208/7‑101 (MQ=255)
                  gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tt                                                                               <  1:4852411/95‑1 (MQ=255)
                   ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.ttt                                                                              <  1:3223141/96‑2 (MQ=255)
                    tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tttg                                                                             >  1:4481657/5‑99 (MQ=255)
                    tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tttg                                                                             >  2:2398772/5‑99 (MQ=255)
                     ggtgtcggtcTTGGTGATTAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tttgc                                                                            <  2:1909840/98‑4 (MQ=255)
                     ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tttgc                                                                            <  2:649410/98‑4 (MQ=255)
                     ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tttgc                                                                            <  2:3447890/98‑4 (MQ=255)
                     ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tttgc                                                                            >  1:5344879/4‑98 (MQ=255)
                     ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tttgc                                                                            >  2:368603/4‑98 (MQ=255)
                     ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tttgc                                                                            <  2:4188460/98‑4 (MQ=255)
                     ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tttgc                                                                            >  2:3952694/4‑98 (MQ=255)
                      gtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tttgcg                                                                           >  2:3049584/3‑97 (MQ=255)
                      gtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tttgcg                                                                           <  1:3671023/99‑5 (MQ=255)
                       tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tttgcgc                                                                          <  2:1483106/100‑6 (MQ=255)
                       tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAG.tttgcgc                                                                          <  1:1725660/100‑6 (MQ=255)
                        gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGca                                                                          >  1:1279570/1‑101 (MQ=255)
                        gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGca                                                                          >  2:2992303/1‑101 (MQ=255)
                        gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGca                                                                          >  2:2844219/1‑101 (MQ=255)
                         tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGcat                                                                         <  1:915617/101‑1 (MQ=255)
                         tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGcat                                                                         <  2:5316771/101‑1 (MQ=255)
                          cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGcatc                                                                        >  1:1369569/1‑101 (MQ=255)
                             tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCatcatc                                                                     >  2:2262382/11‑101 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCAc                                                                   <  1:3921805/93‑1 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCAc                                                                   <  1:4392804/93‑1 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCAc                                                                   <  1:4703446/93‑1 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCAc                                                                   >  2:3712874/9‑101 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCAc                                                                   <  1:2590915/93‑1 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCAc                                                                   <  1:1936323/93‑1 (MQ=255)
                                 ggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACgg                                                                 <  1:3259828/95‑1 (MQ=255)
                                   tgatcagacagGCCCCTGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGtc                                                               <  2:4822995/97‑1 (MQ=255)
                                    gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGtct                                                              <  2:5416603/98‑1 (MQ=255)
                                     atcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGtctc                                                             >  1:5164820/3‑101 (MQ=255)
                                        agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTctcc                                                          >  2:2960200/1‑101 (MQ=255)
                                        agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTctcc                                                          >  1:3410000/1‑101 (MQ=255)
                                          acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTctcctc                                                        >  1:1037473/1‑101 (MQ=255)
                                           cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCtcctcc                                                       <  1:3709795/101‑1 (MQ=255)
                                           cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCtcctcc                                                       <  2:2601139/101‑1 (MQ=255)
                                           cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCtcctcc                                                       <  1:4645659/101‑1 (MQ=255)
                                           cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCtcctcc                                                       >  2:1754358/1‑101 (MQ=255)
                                            agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTccc                                                      >  2:5433264/1‑101 (MQ=255)
                                            agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTccc                                                      >  1:4764813/1‑101 (MQ=255)
                                             ggCCCGGGGTGCGGCCGCAGCCGCCCCGGCCAGCGCGGAAAGCGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCg                                                     <  1:4296340/101‑1 (MQ=255)
                                              gCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGt                                                    <  2:4687457/101‑1 (MQ=255)
                                                cccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt                                                  >  2:1809489/1‑101 (MQ=255)
                                                 ccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                                 >  1:5100170/1‑101 (MQ=255)
                                                 ccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                                 >  1:2932507/1‑101 (MQ=255)
                                                  cGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                                >  2:4502769/1‑101 (MQ=255)
                                                    gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                              >  2:1564857/1‑101 (MQ=255)
                                                    gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                              <  2:250440/101‑1 (MQ=255)
                                                    gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                              >  1:624817/1‑101 (MQ=255)
                                                    gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                              <  2:3830431/101‑1 (MQ=255)
                                                     tttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg                                             >  2:2136670/1‑101 (MQ=255)
                                                         ggCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg                                         >  2:49115/1‑101 (MQ=255)
                                                         ggCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.CCCCAACCGGGCCCGG.TTGCGCCTCATCCAGGGCTCTCCTCCCGTTTCACTGTCGGc                                        >  2:2661340/1‑101 (MQ=255)
                                                           ccgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcc                                       <  2:4411483/97‑1 (MQ=255)
                                                            cgaaGCCGCCCCGGCCAGCGCGGAGAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                                      <  1:2543797/98‑1 (MQ=255)
                                                            cgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                                      >  1:3634032/4‑101 (MQ=255)
                                                            cgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                                      <  1:3579320/98‑1 (MQ=255)
                                                             gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     >  2:605334/3‑101 (MQ=255)
                                                             gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     >  2:857208/3‑101 (MQ=255)
                                                              aaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                                    <  2:676588/100‑1 (MQ=255)
                                                                  cgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCctcctc                                <  1:3049758/101‑1 (MQ=255)
                                                                   gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                               >  2:307788/1‑101 (MQ=255)
                                                                   gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                               >  2:4565075/1‑101 (MQ=255)
                                                                   gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                               <  1:527058/101‑1 (MQ=255)
                                                                   gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                               >  1:2727838/1‑101 (MQ=255)
                                                                    ccccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACTGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCg                              <  2:551046/101‑1 (MQ=255)
                                                                    ccccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCg                              <  2:4322764/101‑1 (MQ=255)
                                                                    ccccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCg                              <  1:763197/101‑1 (MQ=255)
                                                                        ggCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc                          <  1:1877466/101‑1 (MQ=255)
                                                                           cAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCg                       <  1:4329906/101‑1 (MQ=255)
                                                                           cAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCg                       >  2:5382026/1‑101 (MQ=255)
                                                                           cAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCg                       <  2:4092389/101‑1 (MQ=255)
                                                                              cgcgGAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGAc                    <  2:1093121/101‑1 (MQ=255)
                                                                                 ggAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                 <  2:594584/101‑1 (MQ=255)
                                                                                  gAAAACGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAg                >  2:1896406/1‑101 (MQ=255)
                                                                                   aaaaCGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGt               >  1:3156083/1‑101 (MQ=255)
                                                                                      aCGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg            >  2:323730/1‑101 (MQ=255)
                                                                                      aCGCCAGGGCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg            >  2:1185103/1‑101 (MQ=255)
                                                                                          cagggCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAccc        >  1:3375732/4‑101 (MQ=255)
                                                                                           agggCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCg       >  2:2827825/3‑101 (MQ=255)
                                                                                             ggCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg     <  1:4084764/101‑1 (MQ=255)
                                                                                             ggCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg     <  1:3009175/101‑1 (MQ=255)
                                                                                             ggCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg     >  2:2426566/1‑101 (MQ=255)
                                                                                              gCAGAGGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGt    >  1:1070310/1‑101 (MQ=255)
                                                                                               cAGAGGCCCCCAGCAGGG.CCAGTTTGCGCATCATCACGGTCTCACCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTc   <  2:5164534/101‑1 (MQ=255)
                                                                                                agagGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg  >  1:2019057/1‑101 (MQ=255)
                                                                                                agagGCCCCCAACAGGG.CCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg  >  2:5643662/1‑101 (MQ=255)
                                                                                                     |                                                                                                
CATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGG.CCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCG  >  NC_014034/2177257‑2177444

Base quality scores:  ATCG  < 3 ≤  ATCG  < 26 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG