BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,486,428	+G	98.4%	intergenic (‑56/+138)	RCAP_rcc02312 ← / ← soxG	hypothetical protein/sarcosine oxidase subunit gamma

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,486,428	1	.→G	98.4%	3.8 ‑1.1 ‑1.3	248	intergenic (‑56/+138)	RCAP_rcc02312/soxG	hypothetical protein/sarcosine oxidase subunit gamma
Reads supporting (aligned to +/- strand): new base (109/135): ref base (3/1): total (112/136)
Fisher's exact test strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.91e-02

Only 100 of 293 total aligned reads displayed.


ATTGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.A.GGGG.CGCTGCCCCTCTCTTG.CC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGG  >  NC_014034/2486328‑2486525


aTTGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.aaa.a                                                                                                       >  2:5083056/1‑101 (MQ=255)


 ttGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.A.g                                                                                                     <  1:3423515/101‑1 (MQ=255)


  tGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.A.gg                                                                                                    >  1:387079/1‑101 (MQ=255)


  tGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.A.gg                                                                                                    >  1:896287/1‑101 (MQ=255)


    ttCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.A.gggg                                                                                                  <  2:4829588/101‑1 (MQ=255)


     tCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.A.ggggg                                                                                                 >  2:4896363/1‑100 (MQ=255)


      cTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.A.gggggc                                                                                                <  2:397062/101‑3 (MQ=255)


      cTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.A.gggggc                                                                                                <  1:4736275/101‑3 (MQ=255)


       ttGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.A.gggggcg                                                                                               >  2:3634614/1‑98 (MQ=255)


         gTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.A.gggggcgct                                                                                             >  1:3647950/1‑96 (MQ=255)


            gggCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGcc                                                                                          >  2:2957371/1‑101 (MQ=255)


            gggCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGcc                                                                                          >  2:4142055/1‑101 (MQ=255)


             ggCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGccc                                                                                         >  1:5297116/1‑101 (MQ=255)


             ggCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGccc                                                                                         >  1:674080/1‑101 (MQ=255)


              gCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGcccc                                                                                        >  2:82228/1‑101 (MQ=255)


                cgcgcgCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGccccct                                                                                      <  2:2160770/101‑3 (MQ=255)


                 gcgcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGccccctc                                                                                     >  2:4581983/1‑98 (MQ=255)


                 gcgcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGccccctc                                                                                     <  1:2610392/101‑4 (MQ=255)


                  cgcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAACG.GGGG.CGCTGccccctct                                                                                    <  2:1732219/101‑5 (MQ=255)


                   gcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCtt                                                                                  <  2:4666719/101‑1 (MQ=255)


                     gcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTtg.c                                                                               >  2:5591539/1‑101 (MQ=255)


                     gcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTtg.c                                                                               <  2:682299/101‑1 (MQ=255)


                     gcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTtg.c                                                                               >  1:38155/1‑101 (MQ=255)


                      cccccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTtg.cc                                                                              <  1:4616433/101‑1 (MQ=255)


                       ccccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTtg.ccc                                                                             >  1:2453959/1‑100 (MQ=255)


                       ccccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.A.GGGG.GGCGCGCCCCCCCTtg.ccc                                                                             >  2:1976311/1‑100 (MQ=255)


                        cccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTtg.ccct                                                                            <  2:876627/101‑3 (MQ=255)


                        cccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTtg.ccct                                                                            <  2:3460238/101‑3 (MQ=255)


                         ccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGTCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTtg.ccctg                                                                           <  2:2432122/101‑4 (MQ=255)


                         ccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTtg.ccctg                                                                           <  1:3266266/101‑4 (MQ=255)


                           ggATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTtg.ccctgcc                                                                         >  2:2496716/1‑96 (MQ=255)


                           ggATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTtg.ccctgcc                                                                         >  1:102602/1‑96 (MQ=255)


                            gATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCg                                                                        >  1:1751941/1‑101 (MQ=255)


                             aTTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCgg                                                                       >  1:2750889/1‑101 (MQ=255)


                              tttCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGCAAA.G.GGGG.GGCTGCCCC.CTCTtg.ccctgcccgg                                                                      >  1:2284375/1‑93 (MQ=255)


                              tttCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCggg                                                                      >  2:3820248/1‑101 (MQ=255)


                               ttCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGc                                                                     <  1:407671/101‑1 (MQ=38)


                               ttCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGc                                                                     <  2:3928349/101‑1 (MQ=38)


                                tCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCa                                                                    >  1:3087004/1‑101 (MQ=38)


                                tCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCa                                                                    >  1:1873242/1‑101 (MQ=38)


                                  gAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCATGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAg                                                                  >  2:5002743/1‑101 (MQ=37)


                                    aTAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTc                                                                <  2:3149755/101‑1 (MQ=38)


                                    aTAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTc                                                                >  1:964701/1‑101 (MQ=38)


                                      aGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCAc                                                              >  2:2852455/1‑101 (MQ=38)


                                      aGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCAc                                                              >  2:1118060/1‑101 (MQ=38)


                                        tCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCAccc                                                            >  1:2871834/1‑101 (MQ=37)


                                        tCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCAccc                                                            >  1:1055575/1‑101 (MQ=37)


                                         ccATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCAcccc                                                           >  1:2662702/1‑101 (MQ=37)


                                          cATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCAccccc                                                          >  1:1643430/1‑101 (MQ=37)


                                             gCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCggg                                                       <  2:375947/101‑1 (MQ=37)


                                              cTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGa                                                      <  1:2187106/101‑1 (MQ=37)


                                                 gATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGatat                                                   >  1:601403/1‑101 (MQ=37)


                                                   tctcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTc                                                 <  1:4190317/101‑1 (MQ=37)


                                                   tctcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTc                                                 <  2:3902453/101‑1 (MQ=37)


                                                   tctcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTc                                                 >  2:5509644/1‑101 (MQ=37)


                                                     tcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCAt                                               <  2:2383755/101‑1 (MQ=37)


                                                     tcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCGGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCAt                                               <  1:5188820/101‑1 (MQ=38)


                                                      ccTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATg                                              <  1:4522493/101‑1 (MQ=37)


                                                      ccTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGACATCCAtc                                              >  1:5797961/1‑100 (MQ=25)


                                                       cTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATgg                                             >  2:4470391/1‑101 (MQ=37)


                                                       cTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATgg                                             >  1:2994452/1‑101 (MQ=37)


                                                       cTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATgg                                             >  2:910978/1‑101 (MQ=37)


                                                        tttGCCTTCGATCTGGGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGCCCCCCCCGGGATATTCATGGc                                            <  1:4258251/101‑1 (MQ=25)


                                                         ttGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCa                                           <  1:3506684/101‑1 (MQ=37)


                                                         ttGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCa                                           <  2:3409461/101‑1 (MQ=37)


                                                         ttGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCa                                           <  1:2360003/101‑1 (MQ=37)


                                                          tGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCaa                                          >  2:1615125/1‑101 (MQ=37)


                                                             cTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGaa                                       >  1:564210/1‑100 (MQ=37)


                                                             cTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGAt                                       >  1:45516/1‑101 (MQ=37)


                                                             cTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGAt                                       <  2:5711169/101‑1 (MQ=37)


                                                               tCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCCCCCCCCGGATATTCATGGCACGATGa                                     >  1:1082875/1‑101 (MQ=25)


                                                               tCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGa                                     >  1:4387033/1‑101 (MQ=37)


                                                                 gATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAag                                   >  1:1410468/1‑101 (MQ=37)


                                                                  aTCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAagg                                  >  1:5483593/1‑101 (MQ=37)


                                                                   tgtGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAagga                                 <  2:2284249/99‑1 (MQ=25)


                                                                    cTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAaggag                                >  1:4333171/1‑101 (MQ=25)


                                                                     tGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAaggagg                               <  2:1304570/101‑1 (MQ=37)


                                                                     tGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAaggagg                               >  1:3159744/1‑101 (MQ=25)


                                                                            aGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGttt                        <  2:4367863/101‑1 (MQ=37)


                                                                            aGCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGttt                        >  1:4132557/1‑101 (MQ=25)


                                                                             gCCCGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTc                       <  1:496489/101‑1 (MQ=25)


                                                                              cccGGCCGCAAGGCAAGCG.AAA.A.GGGGTCTCTGCCCC.CTCTTGCCC.TGCCGTGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCa                      <  2:2769744/101‑1 (MQ=255)


                                                                                cGGCCGCAAGGCAAGCG.AAA.AGGGGG.CTCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATc                    <  2:582235/101‑1 (MQ=25)


                                                                                cGGCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATc                    <  2:3311503/101‑1 (MQ=25)


                                                                                 ggCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCt                   >  1:1197034/1‑101 (MQ=25)


                                                                                 ggCCGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCt                   <  1:3444410/101‑1 (MQ=37)


                                                                                   ccGCAAGGCAAGCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTg                 <  2:2662553/101‑1 (MQ=25)


                                                                                     gcaaggcaagCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGcc               <  2:5392270/101‑1 (MQ=25)


                                                                                      caaggcaagCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCa              <  1:5157075/101‑1 (MQ=21)


                                                                                       aaggcaagCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCAt             >  2:1059505/1‑101 (MQ=21)


                                                                                        aggcaagCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATa            >  2:4209701/1‑101 (MQ=21)


                                                                                         ggcaagCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATaa           >  1:3727199/1‑101 (MQ=21)


                                                                                          gcaagCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATaaa          >  2:3080264/1‑101 (MQ=21)


                                                                                           caagCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAat         <  1:812779/101‑1 (MQ=25)


                                                                                            aagCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAata        >  2:2315278/1‑101 (MQ=21)


                                                                                             agCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAatat       <  1:2133788/101‑1 (MQ=21)


                                                                                              gCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATc      <  2:2871673/101‑1 (MQ=21)


                                                                                              gCG.AAA.AGGGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATc      >  2:3768830/1‑101 (MQ=21)


                                                                                                 aaaa.g.GGGG.CGCTGCCCC.CTCTTG.CCTTGCCGGGCAAGTCACCCCCGGGGTATTCATGGCAAGATGAAGGAGGCGTTTTTCATCTTGCCATCAAAATCCCg   >  1:1326126/2‑101 (MQ=25)


                                                                                                  aaa.g.GGGG.CGCTGCCCC.CTCTTGCCC.TGCCGGGCAAGTCACCCCCGGGATATTCATTGCAACATGAAGGCGGCGTGTTTACTCTTGCCATAAATGTCCCgg  >  2:1402455/1‑101 (MQ=21)


ATTGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCG.AAA.A.GGGG.CGCTGCCCCTCTCTTG.CC.TGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGG  >  NC_014034/2486328‑2486525

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG