Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 257,088 +G 1.8% intergenic (+22/‑177) secA → / → RCAP_rcc00221 preprotein translocase subunit SecA/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034257,0881.→G 1.8% 4.4 ‑3.4 ‑0.3224intergenic (+22/‑177)secA/RCAP_rcc00221preprotein translocase subunit SecA/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (92/128):  total (96/128)
Fisher's exact test strand distribution p-value = 3.25e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.47e-01

Only 100 of 249 total aligned reads displayed.

GGGCGAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GCCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCG  >  NC_014034/256988‑257186
                                                                                                     |                                                                                                     
 ggCGAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCCCGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGgc.g                                                                                                      >  1:1415270/1‑101 (MQ=255)
   cGAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGgc.ggc                                                                                                    <  1:823089/101‑3 (MQ=255)
     aGCCCTCGCGCAATGCCCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCgg                                                                                                  <  2:3920915/101‑1 (MQ=255)
      gCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGt                                                                                                 <  1:4771700/101‑1 (MQ=255)
        ccTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGG.cc                                                                                              >  2:4081380/1‑101 (MQ=255)
         cTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCg                                                                                             <  1:3785243/101‑1 (MQ=255)
           cgcgCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCggg                                                                                           <  2:4921948/101‑1 (MQ=255)
             cgcAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGt                                                                                         <  2:803377/101‑1 (MQ=255)
             cgcAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGt                                                                                         <  2:4308110/101‑1 (MQ=255)
             cgcAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGt                                                                                         <  2:4166471/101‑1 (MQ=255)
             cgcAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGt                                                                                         <  1:4096512/101‑1 (MQ=255)
              gcAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTg                                                                                        <  1:4081627/101‑1 (MQ=255)
                aaggACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGG.CCggggggga                                                                                      >  1:3303858/4‑97 (MQ=255)
                aaTGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGG.CCcgggggga                                                                                      >  2:935861/1‑97 (MQ=255)
                 aTGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGG.CCgggggggac                                                                                     >  1:5448372/1‑96 (MQ=255)
                   gACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGGAccc                                                                                   >  2:1619275/1‑101 (MQ=255)
                    aCCCCTCCCCCTGCGGTTGGGGCGAGAAGTTCAAGCACTGCCACGGACGTCTGGCCTGAAACAAAGGCGGTGAAATATGGC.GGCCGT.CCGGGGTGAACCCg                                                                                  <  2:3726852/101‑1 (MQ=255)
                      ccctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCggg                                                                                <  1:3752560/101‑1 (MQ=255)
                       cctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGGGGGG.CGGGGGGGAACCCggc                                                                                >  2:2222595/1‑100 (MQ=255)
                       cctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGGGGGG.CCGGGGGGAACCCggc                                                                                >  1:2717792/1‑100 (MQ=255)
                       cctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGT.CGGGGGGGACCCCggg                                                                                >  1:1032791/1‑101 (MQ=255)
                       cctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGgc                                                                               <  1:1619369/101‑1 (MQ=255)
                        ctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGgcc                                                                              <  1:2609598/101‑1 (MQ=255)
                        cnnccnctgcGGCTAGGNCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGgcc                                                                              <  2:1497403/101‑1 (MQ=255)
                          gcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGG.GCGGGGGGGACCCCGggcg.c                                                                           >  2:3175399/1‑101 (MQ=255)
                           cccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGgccg.cc                                                                          <  1:5660888/101‑1 (MQ=255)
                            ccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGccg.ccg                                                                         <  2:3357304/101‑1 (MQ=255)
                             cctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGT.CCGGGGGGGACCCGGGgcg.ccgg                                                                        >  2:863804/1‑100 (MQ=255)
                              ctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGGGGGT.CGGGGGGGAACCCCGgcgccgct                                                                         >  2:841717/1‑95 (MQ=255)
                              ctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGG.GCGGGGGGGGACCGGGgcg.ccggt                                                                       >  1:2125953/1‑99 (MQ=255)
                               tgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGttt                                                                      <  2:4472563/101‑1 (MQ=255)
                               tgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGttt                                                                      <  1:2570336/101‑1 (MQ=255)
                               tgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGttt                                                                      <  2:1817540/101‑1 (MQ=255)
                                 cGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGttttt                                                                    <  2:306215/101‑1 (MQ=255)
                                  ggCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTc                                                                   <  1:1237121/101‑1 (MQ=255)
                                   gCTCGGGCGAGAAGGTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCa                                                                  <  2:1858550/101‑1 (MQ=255)
                                      cGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGG.CCGGGGGGGACCCCGGGCG.CCGGTTTTCCttt                                                               >  2:2923099/1‑100 (MQ=255)
                                       gggCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGG.CCGGGGGGAACCCGGGCCG.CCGGTTTTTATTAt                                                              >  2:2942277/1‑101 (MQ=255)
                                        ggCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGAAACCCGGCCG.CCGGTTTTCATTATc                                                             >  2:4389021/1‑101 (MQ=255)
                                         gCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGGGGGG.CGGGGGGGAACCGGGGCCG.CCGTTTTTCTTTTTc                                                             >  2:2028654/1‑101 (MQ=255)
                                         gCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGG.CCGGGGGGAACCCCGGCCG.CCGGTTTTTATTATcc                                                            >  1:5275775/1‑101 (MQ=255)
                                          cGAGAAGTTCAAGCACTGCCACGGCGCTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCt                                                           <  1:4858672/101‑1 (MQ=255)
                                          cGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGAC.GGCGGG.CCCGGGGGGAACCGGGGCG.CCGTTTTTTATTATccc                                                           >  1:359801/1‑100 (MQ=255)
                                           gagaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCGGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTg                                                          <  1:2995354/101‑1 (MQ=255)
                                            agaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGt                                                         <  2:3008833/101‑1 (MQ=255)
                                             gaAGTTCAAGCGCTGCCCCGGCCGTCTGGCCTGAAACAAAGTCGCTCAAATAGGGC.GGGGGT.CCGGGGTGCACCCGGGCCG.CCGTTTTTCATTATCCTGtt                                                        <  1:3648204/101‑1 (MQ=255)
                                             gaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGG.CCGGGGGGAACCCGGGGCGCCCGTTTTTCTTTTTctttt                                                         >  2:5077579/1‑97 (MQ=255)
                                              aaGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGG.CCGGGGGGAACCCGGGCCG.CCGGTTTTTCTTATCCTTTTg                                                       >  1:601156/1‑101 (MQ=255)
                                               aGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGa                                                      <  1:863880/101‑1 (MQ=255)
                                                   cAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTg                                                  <  1:2015138/101‑1 (MQ=255)
                                                      gCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGat                                               <  2:189068/101‑1 (MQ=255)
                                                      gCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGat                                               <  2:4503720/101‑1 (MQ=255)
                                                       cACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGat.a                                             <  1:4438176/101‑1 (MQ=255)
                                                       cACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGat.a                                             <  1:1501106/101‑1 (MQ=255)
                                                       cACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGat.a                                             <  1:3514037/101‑1 (MQ=255)
                                                       cACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGG.CCGGGGGGAACCCCGGCCG.GCGGTTTTTATTATTCTTtttttttgggtt                                              >  2:5478604/1‑91 (MQ=255)
                                                        aCTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGAACCCGGGCCG.CCGGTTTTTATTATCCTGTTGATCTGGat.at                                            >  1:5129305/1‑101 (MQ=255)
                                                         cTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATg                                           <  1:3128201/101‑1 (MQ=255)
                                                          tGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGGGGTCCCGGGGGGAAACCGGGCGG.CGGTTTTT.ATTTTTCTGTTTTACTGGat.attt                                          >  2:1739713/1‑99 (MQ=255)
                                                            ccACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGttt                                        <  2:2588655/101‑1 (MQ=255)
                                                             cACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGtttt                                       <  2:4017755/101‑1 (MQ=255)
                                                              aCGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGAAACCGGGCCG.CCGCTTTTTATTTTCCTGTTGATCTGGAT.AAGttttt                                      >  2:4989866/1‑101 (MQ=255)
                                                                ggCCGTCTGGATTCAATCAAAGTCGCAGAAATAGGGC.ATAAGC.TCGCGGTGCGCCCGGGCCG.CCGTTTTTCATTATCCTGTTGAACTTGAT.ATGTTTTtgt                                    <  2:1334073/101‑1 (MQ=255)
                                                                ggCCGTCGGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTtgt                                    <  2:323450/101‑1 (MQ=255)
                                                                 gCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTtgtg                                   <  1:5231209/101‑1 (MQ=255)
                                                                  ccGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTgg                                  <  1:2909732/101‑1 (MQ=255)
                                                                   cGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGa                                 <  2:2717643/101‑1 (MQ=255)
                                                                   cGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGa                                 <  1:1158424/101‑1 (MQ=255)
                                                                   cGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGAACCCGGGCCG.CCGTTTTTTCTTATCCTGTTGATCTGGAT.ATGTTTTTTTGGa                                 >  2:3683544/1‑101 (MQ=255)
                                                                    gTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGG.CCGGGGGGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTTTggga                                >  2:1038963/1‑99 (MQ=255)
                                                                     tCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGAACCCGGGCCG.CCGGTTTTTATTATCCTGTTTATCTGGAT.ATGTTTTTGTGGaaa                               >  1:1143676/1‑101 (MQ=255)
                                                                     tCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGG.CCGGGGGGAACCCGGGCCG.CCGGTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTTTGGaaa                               >  1:390460/1‑101 (MQ=255)
                                                                       tGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAAtt                             <  2:4627969/101‑1 (MQ=255)
                                                                       tGGCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGAACCCGGGCCG.CCGGTTTTCATTATCCTGTTGATCTGGGT.ATGTTTTTTTGGAAAtt                             >  2:5822/1‑101 (MQ=255)
                                                                        ggCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAAttt                            <  1:2317033/101‑1 (MQ=255)
                                                                        ggCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAAttt                            <  1:5754416/101‑1 (MQ=255)
                                                                        ggCCGGAAACAAAGGCGCTGAAATATGGC.GGCGTT.CGGGGGTGAACCCGGCCCG.CCGTTTTTTATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAAttt                            <  2:2554975/101‑1 (MQ=255)
                                                                         gCCTGAAACAAAGGCGCTGAAATATGGC.GGTGGG.CCGGGGGGAACCCGGGCCG.CCCGTTTTTATTTTTCTGTTGTTTTGGGTGTTGTTTTGGTGGAATttc                            >  1:2562043/1‑100 (MQ=255)
                                                                         gCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTc                           <  1:3257022/101‑1 (MQ=255)
                                                                         gCCTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGAACCCCGGGCG.CCGTTTTTTATTATCCTGTTTATCTGGGT.ATGTTTTTTTGGAAAtttt                           >  1:90298/1‑100 (MQ=255)
                                                                          ccTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTcc                          <  1:4239291/101‑1 (MQ=255)
                                                                          ccTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTcc                          <  1:4370706/101‑1 (MQ=255)
                                                                          ccTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTTTGGAAATTTcc                          >  1:5510748/1‑101 (MQ=255)
                                                                          ccTGAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTcc                          >  2:3548466/1‑101 (MQ=255)
                                                                          ccTGAAACAAAGGCGCTGAAATATGGC.GGCGGG.CCGGGGGGAACCCCGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTcc                          >  1:1779916/1‑101 (MQ=255)
                                                                             gAAACAAAGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTTTGGAAATTTccggc                       >  1:3629026/1‑101 (MQ=255)
                                                                                  aaaGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTTTGGAAATTTCCCGCCGGGc                  >  1:1464458/1‑101 (MQ=255)
                                                                                  aaaGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGAACCCCGGGCG.CCGGTTTTCATTATCCTGTTGATCCGGGT.ATGTTTTTTTGGAAATTTCCGGCCGGGc                  >  1:4573800/1‑101 (MQ=255)
                                                                                    aGGCGCTGAAATATGGC.GGCGGT.CCGGGGGGAACCCGGGCCG.CCGTTTTTCATTATCCCGTTGATCTGGAT.ATGTTTTTTTGGAAATTTCCGGCCGGGCGa                >  2:4663120/1‑101 (MQ=255)
                                                                                      gcgcTGAAATAGGGC.GGGGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTCCGGCCGGGCGAAg              <  2:4880592/101‑1 (MQ=255)
                                                                                       cgcTGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTCCGGCCGGGCGAAgg             <  2:1456549/101‑1 (MQ=255)
                                                                                       cgcTGAAATATGGC.GGCGGT.CCGGGGGGAACCCGGGGCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTTTGGAAATTTCCGGCCGGGCGAAgg             >  1:5029668/1‑101 (MQ=255)
                                                                                       cgcTGAAATATGGC.GGCGGT.CCGGGGGGAACCCGGGCCG.CCGGTTTTTATTATTCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTTCGGCCGGGCGGAgg             >  2:614061/1‑101 (MQ=255)
                                                                                          tGAAATATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGttt          <  1:3388107/101‑1 (MQ=255)
                                                                                          tGAAATATGGC.GGCGGT.CCGGGGGGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGttt          >  2:5336986/1‑101 (MQ=255)
                                                                                            aaaTATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTcc        <  2:4398866/101‑1 (MQ=255)
                                                                                             aaTATGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTTTCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCg       >  1:3961707/1‑101 (MQ=255)
                                                                                                 tGGC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTc   >  2:2443380/1‑101 (MQ=255)
                                                                                                  ggC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCg  >  2:3949264/1‑101 (MQ=255)
                                                                                                  ggC.GGCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCg  >  1:2235468/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
GGGCGAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGC.GCCGGT.CCGGGGTGAACCCGGGCCG.CCGTTTTTCATTATCCTGTTGATCTGGAT.ATGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCG  >  NC_014034/256988‑257186

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG