Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,577,283 +G 100% intergenic (+205/‑224) fdx → / → purU ferredoxin VI/formyltetrahydrofolate deformylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,577,2831.→G100.0% 12.7 86intergenic (+205/‑224)fdx/purUferredoxin VI/formyltetrahydrofolate deformylase
Reads supporting (aligned to +/- strand):  new base (69/11):  ref base (1/5):  total (70/16)
Fisher's exact test strand distribution p-value = 6.67e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.18e-01

Only 100 of 116 total aligned reads displayed.

CGGCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.GG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGAGCA  >  NC_014034/2577183‑2577377
                                                                                                       |                                                                                                  
cGGCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTc.c                                                                                                     <  2:3736752/101‑1 (MQ=255)
cGGCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTc.c                                                                                                     <  2:668906/101‑1 (MQ=255)
cGGCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTc.c                                                                                                     <  1:540951/101‑1 (MQ=255)
cGGCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTc.c                                                                                                     <  1:2332068/101‑1 (MQ=255)
 ggCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.g                                                                                                   <  2:330385/101‑1 (MQ=255)
 ggCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.g                                                                                                   <  2:4637875/101‑1 (MQ=255)
 ggCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.g                                                                                                   <  2:5137251/101‑1 (MQ=255)
  gCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.gg                                                                                                  >  1:5308080/1‑101 (MQ=255)
  gCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.gg                                                                                                  <  2:4572706/101‑1 (MQ=255)
  gCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.gg                                                                                                  >  2:1044967/1‑101 (MQ=255)
  gCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.gg                                                                                                  >  2:2523777/1‑101 (MQ=255)
   cGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.gg.g                                                                                                >  2:2077434/1‑101 (MQ=255)
    gTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATACC.GGGGGTC.C.gg.g.g                                                                                              >  2:376272/1‑101 (MQ=255)
     tATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGTCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.gg.g.gg                                                                                             <  1:4962032/101‑2 (MQ=255)
      aTTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.gg.g.ggc                                                                                            >  1:1549076/1‑99 (MQ=255)
        ttGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.gg.g.ggcgg                                                                                          <  1:4856566/101‑5 (MQ=255)
        ttGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGGC.C.gg.g.ggcgg                                                                                          <  1:1251226/101‑5 (MQ=255)
          gCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCCTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAg                                                                                         <  1:4518755/101‑1 (MQ=255)
           cACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGc                                                                                        <  2:1561528/101‑1 (MQ=255)
            aCCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGcc                                                                                       >  2:1824115/1‑101 (MQ=255)
              cAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGcccc                                                                                     >  1:712384/1‑101 (MQ=255)
               aGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGccccc                                                                                    >  1:4480316/1‑101 (MQ=255)
                ggAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCc..g                                                                                 >  2:1604160/1‑101 (MQ=255)
                 gAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGGC.CGGG.G.GCGGAGGCCCc..gg                                                                                >  1:2252461/1‑101 (MQ=38)
                   aaagccaaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCc..ggcg                                                                              >  1:3131576/1‑101 (MQ=255)
                   aaagccaaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCc..ggcg                                                                              >  2:5549145/1‑101 (MQ=255)
                    aagccaaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCc..ggcgg                                                                             >  2:4770386/1‑101 (MQ=255)
                     agccaaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCggg                                                                            <  2:2053776/101‑1 (MQ=255)
                      gccaaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCgggg                                                                           >  1:4084637/1‑100 (MQ=255)
                      gccaaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGt                                                                           >  2:4810098/1‑101 (MQ=255)
                      gccaaagccGCCCCCGGCAGGACCGAAGGCCTCAGGCCCCCGGGCCCCTTTTATCTTGCCAAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGt                                                                           <  2:4186108/101‑1 (MQ=25)
                        caaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCg                                                                         >  2:4308225/1‑101 (MQ=255)
                        caaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCg                                                                         >  1:2995260/1‑101 (MQ=255)
                         aaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATGTCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGGCGc                                                                        >  1:4943267/1‑101 (MQ=25)
                          aagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCggc                                                                       >  2:5752462/1‑99 (MQ=255)
                             ccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGACCCCCC..GGCGGGCCGCCggg                                                                    >  1:1366669/1‑101 (MQ=25)
                              cGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGGCGCCgggg                                                                   >  1:4489644/1‑101 (MQ=255)
                               gTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGGGGGCggggg                                                                  >  2:2399115/1‑100 (MQ=25)
                               gTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTATATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGGCGCCGGGGc                                                                  >  2:5316710/1‑101 (MQ=25)
                                 ccccGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGa                                                                >  2:5000958/1‑101 (MQ=255)
                                  cccGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCA..GGCGGGCCGCCGGGGCGaa                                                               >  2:2748547/1‑101 (MQ=25)
                                  cccGGCAGGACCGAAGCCCTCAGCACCCGGGCCCCTTTTTATCTTGCCAAAAATACCCG.GGGGGTCGG.GG.G.GGGGACCCCCC..GGCGGGTCGCCGGGGCGaa                                                               <  2:2895878/101‑1 (MQ=16)
                                       cAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCcgc                                                          >  2:5022854/1‑101 (MQ=255)
                                        cggACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCcgcg                                                         <  1:1696561/100‑1 (MQ=255)
                                        aGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGGGAAGCcgcg                                                         >  1:2719029/1‑101 (MQ=255)
                                        aGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGCCGCCCGCGGGCAGCcgcc                                                         >  2:2339451/1‑100 (MQ=18)
                                             cGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGaa                                                    >  2:116375/1‑101 (MQ=255)
                                               aaGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGGCGCGGCGAAAt                                                  >  2:17253/1‑101 (MQ=255)
                                                 gcccTCAGGCCCCCGGCCCCCTTTTACCTTGCCCAAAATCCCCC.GGGGGTC.T.GGTG.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATcc                                                <  1:1488800/99‑1 (MQ=21)
                                                    cTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATcccgg                                             >  1:2292614/1‑101 (MQ=255)
                                                      cAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATcccggcc                                           >  1:4114803/1‑101 (MQ=255)
                                                      cAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATcccggcc                                           >  2:3908269/1‑101 (MQ=255)
                                                      cAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCCGCGAAATcccggcc                                           >  2:3080911/1‑101 (MQ=255)
                                                       aGGCCCCCGGGCCCTTTTTATCTTCCCAAAAATATCCG.GGGGGTCGG.GG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATcccggccc                                          <  2:2293947/101‑1 (MQ=18)
                                                       aGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGGGAAATcccggccc                                          >  2:1836525/1‑101 (MQ=255)
                                                       aGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATcccggccc                                          >  1:925560/1‑101 (MQ=255)
                                                         gACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.GG.GCGCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGCAATcccggccgcg                                        >  2:940983/1‑101 (MQ=25)
                                                          aCCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATcccggcccccg                                       >  1:4463402/1‑99 (MQ=255)
                                                            cccGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCa                                     >  2:650033/1‑101 (MQ=255)
                                                              cGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAg                                   <  2:4664068/101‑1 (MQ=255)
                                                               ggcccccTTTTATCTTGCCCAAAATATCCC.GGGGGGCGG.GG.G.GCGGGGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAgc                                  <  2:4237037/98‑1 (MQ=21)
                                                                ggCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAgcg                                 >  2:2376876/1‑101 (MQ=255)
                                                                ggCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAgcg                                 >  1:1479959/1‑101 (MQ=255)
                                                                ggCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAgcg                                 >  1:352538/1‑101 (MQ=255)
                                                                ggCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGGCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAgcg                                 >  2:1232589/1‑101 (MQ=255)
                                                                  ccccTTTTATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGcc                               >  2:4541435/1‑101 (MQ=255)
                                                                    ccTTTTATCTGCCCCAAAATACCCG.GGGGGTC.CGGG.G.GCGGCGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTg                             <  2:1046708/101‑1 (MQ=21)
                                                                      ttttATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCCAAAACGCGGCGAAATCCCCGGCCCCGCAAGCGCCTggg                           >  1:3800386/1‑101 (MQ=18)
                                                                       tttATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGGGCCTGGGc                          >  1:4732039/1‑101 (MQ=255)
                                                                       tttATCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCCGGGCGAAGGCGCGGCGAAATCCCCGCCCCGGCAAGGGCCTgggg                          >  2:2227247/1‑100 (MQ=18)
                                                                          acctgccccAAAATACCCC.GGGGGGCGG.GG.G.GCGGGGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCggg                       <  1:2399249/95‑1 (MQ=21)
                                                                          aTCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGGCGCCGGGGCGAATCCGCGCCGAAATCCCGGCCCCGGCAAGCGCCTGGGCggg                       >  2:3807788/1‑101 (MQ=25)
                                                                           tCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGa                      >  1:4195819/1‑101 (MQ=255)
                                                                           tCTTGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGGCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCCGGGCGGGa                      >  2:2224820/1‑101 (MQ=21)
                                                                            ttctcgccaaaAATCCCGGGGGGTC.C.GG.G.GGGGGGCCCCCCGGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCggg                       <  2:1548982/93‑1 (MQ=18)
                                                                             ttGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGGCGCCGGGGCGAAGCCGCGGCGAAATCCCCGCCCCGGCAAGCGCCTGGGCGGGAAg                    >  2:2715540/1‑101 (MQ=25)
                                                                              tGCCCAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGGCGCCGGGGCGAAGCCGCGGAGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGa                   >  1:5119265/1‑101 (MQ=25)
                                                                                cccAAAATTCCCC.GGGGGCCGG.GG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCgcgcaaggc                 <  2:1631812/101‑9 (MQ=21)
                                                                                 ccAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATcg                <  2:3848269/101‑1 (MQ=255)
                                                                                 ccAAAATATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCAGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCACCTGGGCGGGAAGATcg                >  2:5198774/1‑101 (MQ=25)
                                                                                   aaaaTATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATcgcg              >  2:3392170/1‑101 (MQ=255)
                                                                                   aaaaTATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGGCGCCGGGGGGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATcgcg              >  1:3501751/1‑101 (MQ=25)
                                                                                    aaaTATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATcgcgg             >  2:4086550/1‑100 (MQ=255)
                                                                                    aaaTATCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATcgcgc             >  2:813888/1‑101 (MQ=255)
                                                                                      atatCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGGAGATCgcgcgc           >  2:2939853/1‑101 (MQ=255)
                                                                                      atatCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCCGCCCCGGCAAGCGCCTGGGCGGGAAGATCgcgcgc           >  2:507130/1‑101 (MQ=255)
                                                                                        atCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGGAGATCGCGCGccc         >  1:5554942/1‑101 (MQ=255)
                                                                                         tCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGcccc        >  1:1152485/1‑101 (MQ=255)
                                                                                         tCCC.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGGCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGGAGCGCGCGCCcccg        >  1:2925836/1‑100 (MQ=21)
                                                                                           cc.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCgg      >  2:3611719/1‑101 (MQ=255)
                                                                                           cc.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCCAAGCCGCGGCGAAATCCCCGCCCCGCCGAGCGCCCGGGCGGGGAGCGCACGCGCCCCgg      >  1:5198476/1‑101 (MQ=18)
                                                                                           cc.GGGGCTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCgg      <  1:941059/101‑1 (MQ=255)
                                                                                            c.GGGGGTC.CGGG.G.GCGGAGCCCCC..GGCGGGGCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCCGGGCGGGAAGATCGCGCGCCCCggg     >  1:1117423/1‑100 (MQ=37)
                                                                                             ggggggCC.G.GG.G.GGGGACCCCCG..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGAg    <  1:5752757/100‑1 (MQ=38)
                                                                                              gggggTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGAg    <  2:3450922/101‑1 (MQ=255)
                                                                                              gggggTC.CGGG.G.GCGGAGCCCCC..GGCGGGCCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGGAGATCGCGCGCCCCGGAg    >  1:3010559/1‑101 (MQ=37)
                                                                                               ggggTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGGGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGAGc   >  1:5733399/1‑101 (MQ=255)
                                                                                               ggggTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGAGc   >  1:39501/1‑101 (MQ=255)
                                                                                               ggggTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGAGc   >  1:3991763/1‑101 (MQ=255)
                                                                                                gggTC.CGGG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGAGCa  >  1:3891305/1‑101 (MQ=255)
                                                                                                       |                                                                                                  
CGGCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCCTTTTATCTTGCCCAAAATATCCC.GGGGGTC.C.GG.G.GCGGAGCCCCC..GGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGAGCA  >  NC_014034/2577183‑2577377

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG